In situ growth of hollow Cu2O spheres using anionic vesicles as soft templates

Xiaolin Luo,Zhe Pan,Fei Pei,Zhipeng Jin,Kangkang Miao,Pengfei Yang,Huaming Qian,Qiang Chen,Guodong Feng 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.59 No.-

Geometrically optimizing anionic vesicles were fabricated using two types of anionic surfactants with antipodal molecular configuration. The influence of counterions on the anionic vesicles was systematically investigated to maintain electrostatic interaction between the anionic vesicles and the precursors, as well as to ensure the structural integrity of the vesicle templates. The transcription from vesicles to hollow Cu2O spheres was achieved through an in situ reduction approach. The obtained hollow Cu2O spheres were assembled by abundant nanoparticles around the vesicle interface and showed preferable adsorption capacity for methyl orange in the dark compared with the solid Cu2O spheres synthesized without any surfactants.

Considerations on Security and Trust Measurement for Virtualized Environment

Xiaolin Wang,Yan Sang,Yi Liu,Yingwei Luo 한국정보기술융합학회 2011 JoC Vol.2 No.2

3D-QSAR and Molecular Docking Studies on Benzotriazoles as Antiproliferative Agents and Histone Deacetylase Inhibitors

Xiaolin Li,Jie Fu,Wei Shi,Yin Luo,Xiaowei Zhang,Hailiang Zhu,Hongxia Yu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

Benzotriazole is an important synthetic auxiliary for potential clinical applications. A series of benzotriazoles as potential antiproliferative agents by inhibiting histone deacetylase (HDAC) were recently reported. Threedimensional quantitative structure-activity relationship (3D-QSAR), including comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA), were performed to elucidate the 3D structural features required for the antiproliferative activity. The results of both ligand-based CoMFA model (q2 = 0.647, r2 = 0.968, r2 pred = 0.687) and CoMSIA model (q2 = 0.685, r2 = 0.928, r2 pred = 0.555) demonstrated the highly statistical significance and good predictive ability. The results generated from CoMFA and CoMSIA provided important information about the structural characteristics influence inhibitory potency. In addition, docking analysis was applied to clarify the binding modes between the ligands and the receptor HDAC. The information obtained from this study could provide some instructions for the further development of potent antiproliferative agents and HDAC inhibitors.

Transplantation of Gelatin Microspheres Loaded with Wharton's Jelly Derived Mesenchymal Stem Cells Facilitates Cartilage Repair in Mice

Chen Xiaolin,Huang Sunxing,Niu Yongxia,Luo Mingxun,Liu Haiying,Jiao Yiren,Huang Junjiu 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

Background: Knee osteoarthritis (KOA) is a prevalent chronic joint disease caused by various factors. Mesenchymal stem cells (MSCs) therapy is an increasingly promising therapeutic option for osteoarthritis. However, the chronic inflammation of knee joint can severely impede the therapeutic effects of transplanted cells. Gelatin microspheres (GMs) are degradable biomaterial that have various porosities for cell adhesion and cell–cell interaction. Excellent elasticity and deformability of GMs make it an excellent injectable vehicle for cell delivery. Methods: We created Wharton’s jelly derived mesenchymal stem cells (WJMSCs)-GMs complexes and assessed the effects of GMs on cell activity, proliferation and chondrogenesis. Then, WJMSCs loaded in GMs were transplanted in the joint of osteoarthritis mice. After four weeks, joint tissue was collected for histological analysis. Overexpressing-luciferase WJMSCs were performed to explore cell retention in mice. Results: In vitro experiments demonstrated that WJMSCs loaded with GMs maintained cell viability and proliferative potential. Moreover, GMs enhanced the chondrogenesis differentiation of WJMSCs while alleviated cell hypertrophy. In KOA mice model, transplantation of WJMSCs-GMs complexes promoted cartilage regeneration and cartilage matrix formation, contributing to the treatment of KOA. Compared with other groups, in WJMSCs+GMs group, there were fewer cartilage defects and with a more integrated tibia structure. Tracking results of stable-overexpressing luciferase WJMSCs demonstrated that GMs significantly extended the retention time of WJMSCs in knee joint cavity. Conclusion: Our results indicated that GMs facilitate WJMSCs mediated knee osteoarthritis healing in mice by promoting cartilage regeneration and prolonging cell retention. It might potentially provide an optimal strategy for the biomaterial-stem cell based therapy for knee osteoarthritis. Background: Knee osteoarthritis (KOA) is a prevalent chronic joint disease caused by various factors. Mesenchymal stem cells (MSCs) therapy is an increasingly promising therapeutic option for osteoarthritis. However, the chronic inflammation of knee joint can severely impede the therapeutic effects of transplanted cells. Gelatin microspheres (GMs) are degradable biomaterial that have various porosities for cell adhesion and cell–cell interaction. Excellent elasticity and deformability of GMs make it an excellent injectable vehicle for cell delivery. Methods: We created Wharton’s jelly derived mesenchymal stem cells (WJMSCs)-GMs complexes and assessed the effects of GMs on cell activity, proliferation and chondrogenesis. Then, WJMSCs loaded in GMs were transplanted in the joint of osteoarthritis mice. After four weeks, joint tissue was collected for histological analysis. Overexpressing-luciferase WJMSCs were performed to explore cell retention in mice. Results: In vitro experiments demonstrated that WJMSCs loaded with GMs maintained cell viability and proliferative potential. Moreover, GMs enhanced the chondrogenesis differentiation of WJMSCs while alleviated cell hypertrophy. In KOA mice model, transplantation of WJMSCs-GMs complexes promoted cartilage regeneration and cartilage matrix formation, contributing to the treatment of KOA. Compared with other groups, in WJMSCs+GMs group, there were fewer cartilage defects and with a more integrated tibia structure. Tracking results of stable-overexpressing luciferase WJMSCs demonstrated that GMs significantly extended the retention time of WJMSCs in knee joint cavity. Conclusion: Our results indicated that GMs facilitate WJMSCs mediated knee osteoarthritis healing in mice by promoting cartilage regeneration and prolonging cell retention. It might potentially provide an optimal strategy for the biomaterial-stem cell based therapy for knee osteoarthritis.

A New Analytical Method for Link Slab Analysis

Yufan Luo,Quansheng Yan,Xiaogang Yue,Buyu Jia,Xiaolin Yu,Yuxuan Chen 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

Link slabs have been widely used to reduce the number of expansion joints. However, traditional reinforced concrete link slabs are prone to cracking issues, making it crucial to accurately calculate their internal forces. Currently, the most advanced link slab analysis methods treat the link slab and adjacent span as a rotational spring to simulate their restriction on the girder end rotation. However, this method does not comprehensively consider the link slab's influence, leading to conservative results. To address this issue, this study further considers the influence of girder end deformations on adjacent span, resulting in more accurate calculations of the link slab's internal forces. Additionally, the concept of the girder end rotation reduction coefficient is put forward for the first time, which provides a clearer and more quantified understanding of the link slab's influence. The proposed method is verified by a model test and a site test, which shows a significant improvement in accuracy compared to existing analytical methods. On this basis, a parametric study is conducted to investigate the influence of various parameters on the girder end rotation reduction coefficient, which indicates that span length, girder spacing, and support configuration have the greatest influence.

3D-QSAR and Molecular Docking Studies on Benzotriazoles as Antiproliferative Agents and Histone Deacetylase Inhibitors

Li, Xiaolin,Fu, Jie,Shi, Wei,Luo, Yin,Zhang, Xiaowei,Zhu, Hailiang,Yu, Hongxia Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

Benzotriazole is an important synthetic auxiliary for potential clinical applications. A series of benzotriazoles as potential antiproliferative agents by inhibiting histone deacetylase (HDAC) were recently reported. Three-dimensional quantitative structure-activity relationship (3D-QSAR), including comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA), were performed to elucidate the 3D structural features required for the antiproliferative activity. The results of both ligand-based CoMFA model ($q^2=0.647$, $r^2=0.968$, ${r^2}_{pred}=0.687$) and CoMSIA model ($q^2=0.685$, $r^2=0.928$, ${r^2}_{pred}=0.555$) demonstrated the highly statistical significance and good predictive ability. The results generated from CoMFA and CoMSIA provided important information about the structural characteristics influence inhibitory potency. In addition, docking analysis was applied to clarify the binding modes between the ligands and the receptor HDAC. The information obtained from this study could provide some instructions for the further development of potent antiproliferative agents and HDAC inhibitors.

Hypoxia-Inducible Factor 1α Regulates the Transforming Growth Factor β1/ SMAD Family Member 3 Pathway to Promote Breast Cancer Progression

Jianheng Peng,Xiaolin Wang,Liang Ran,Junlong Song,Rong Luo,Yonghong Wang 한국유방암학회 2018 Journal of breast cancer Vol.21 No.3

Purpose: The transforming growth factor β1 (TGF-β1)/SMAD family member 3 (SMAD3) pathway, and hypoxia-inducible factor 1α (HIF-1α) are two key players in various types of malignancies including breast cancer. The TGF-β1/SMAD3 pathway can interact with HIF-1α in some diseases; however, their interaction in breast cancer is still unknown. Therefore, our study aimed to investigate the interactions between the TGF-β1/SMAD3 pathway and HIF-1α in breast cancer. Methods: Expression of HIF-1α in serum of breast cancer patients and healthy controls was detected by quantitative reverse transcription polymerase chain reaction, and the diagnostic value of HIF-1α for breast cancer was evaluated by receiver operating characteristic curve analysis. Breast cancer cell lines overexpressing SMAD3 and HIF-1α were established. Cell apoptosis and proliferation following different treatments were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide, and cell counting kit-8, respectively. Expression of related proteins was detected by western blot. Results: Serum levels of HIF-1α were higher in breast cancer patients than in normal controls. Both SMAD3 and HIF-1α overexpression inhibited cell apoptosis and promoted cell proliferation. Treatment with inhibitors of HIF-1α and SMAD3 promoted apoptosis in breast cancer cells and inhibited their proliferation. Overexpression of HIF-1α promoted the expression of TGF-β1 and SMAD3, while SMAD3 overexpression did not significantly affect expression of HIF-1α or TGF-β1. Conclusion: HIF-1α serves as an upstream regulator of the TGF-β1/SMAD3 pathway and promotes the growth of breast cancer.

Effects of Starvation on Lipid Metabolism and Gluconeogenesis in Yak

Yu, Xiaoqiang,Peng, Quanhui,Luo, Xiaolin,An, Tianwu,Guan, Jiuqiang,Wang, Zhisheng Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.11

This research was conducted to investigate the physiological consequences of undernourished yak. Twelve Maiwa yak ($110.3{\pm}5.85kg$) were randomly divided into two groups (baseline and starvation group). The yak of baseline group were slaughtered at day 0, while the other group of yak were kept in shed without feed but allowed free access to water, salt and free movement for 9 days. Blood samples of the starvation group were collected on day 0, 1, 2, 3, 5, 7, 9 and the starved yak were slaughtered after the final blood sample collection. The liver and muscle glycogen of the starvation group decreased (p<0.01), and the lipid content also decreased while the content of moisture and ash increased (p<0.05) both in Longissimus dorsi and liver compared with the baseline group. The plasma insulin and glucose of the starved yak decreased at first and then kept stable but at a relatively lower level during the following days (p<0.01). On the contrary, the non-esterified fatty acids was increased (p<0.01). Beyond our expectation, the ketone bodies of ${\beta}$-hydroxybutyric acid and acetoacetic acid decreased with prolonged starvation (p<0.01). Furthermore, the mRNA expression of lipogenetic enzyme fatty acid synthase and lipoprotein lipase in subcutaneous adipose tissue of starved yak were down-regulated (p<0.01), whereas the mRNA expression of lipolytic enzyme carnitine palmitoyltransferase-1 and hormone sensitive lipase were up-regulated (p<0.01) after 9 days of starvation. The phosphoenolpyruvate carboxykinase and pyruvate carboxylase, responsible for hepatic gluconeogenesis were up-regulated (p<0.01). It was concluded that yak derive energy by gluconeogenesis promotion and fat storage mobilization during starvation but without ketone body accumulation in the plasma.

Microbial diversity in the rumen, reticulum, omasum, and abomasum of yak on a rapid fattening regime in an agro-pastoral transition zone

Dan Xue,Huai Chen,Xiaolin Luo,Jiuqiang Guan,Yixin He,Xinquan Zhao 한국미생물학회 2018 The journal of microbiology Vol.56 No.10

The ruminant digestive system harbors a complex gut microbiome, which is poorly understood in the case of the four stomach compartments of yak. High-throughput sequencing and quantitative PCR were used to analyse microbial communities in the rumen, reticulum, omasum, and abomasum of six domesticated yak. The diversity of prokaryotes was higher in reticulum and omasum than in rumen and abomasum. Bacteroidetes predominated in the four stomach compartments, with abundance gradually decreasing in the trend rumen > reticulum > omasum > abomasum. Microorganism composition was different among the four compartments, all of which contained high levels of bacteria, methanogens, protozoa and anaerobic fungi. Some prokaryotic genera were associated with volatile fatty acids and pH. This study provides the first insights into the microorganism composition of four stomach compartments in yak, and may provide a foundation for future studies in this area.

Experimental and Numerical Research on Uplift-Restricted and Slip-Permitted Screw-Shaped Connectors

Yuxuan Chen,Quansheng Yan,Xiaolin Yu,Buyu Jia,Yu Wu,Yufan Luo 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.1

Improving mechanical performance of the negative moment regions of continuous composite beams is essential for composite beam bridges. Uplift-restricted and slip-permitted (URSP) screw-shaped connectors can enhance the crack resistance of a concrete slab through free slip between the concrete slab and the steel beam. Furthermore, URSP screw-shaped connectors are more adaptable to the requirements of rapid construction than URSP T-shaped connectors. To investigate the slip and mechanical behavior of URSP screw-shaped connectors, nine push-out tests with various parameters were conducted. In these tests, the thickness of the foam sleeve wrapped outside the screw exerted a highly signifi cant eff ect on free slip performance. A refi ned 3D nonlinear fi nite element model with the foam’s constitutive model was established using ABAQUS. The stress mechanism and failure modes of the FE models were analyzed. Lastly, to estimate the slip performance of URSP screw-shaped connectors, an equation with a simple form was proposed via mechanism analysis and a regression method.