Family History of Cancer and Head and Neck Cancer Risk in a Chinese Population

Huang, Yu-Hui Jenny,Lee, Yuan-Chin Amy,Li, Qian,Chen, Chien-Jen,Hsu, Wan-Lun,Lou, Pen-Jen,Zhu, Cairong,Pan, Jian,Shen, Hongbing,Ma, Hongxia,Cai, Lin,He, Baochang,Wang, Yu,Zhou, Xiaoyan,Ji, Qinghai,Zho Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: The aim of this study was to investigate whether family history of cancer is associated with head and neck cancer risk in a Chinese population. Materials and Methods: This case-control study included 921 cases and 806 controls. Recruitment was from December 2010 to January 2015 in eight centers in East Asia. Controls were matched to cases with reference to sex, 5-year age group, ethnicity, and residence area at each of the centers. Results: We observed an increased risk of head and neck cancer due to first degree family history of head and neck cancer, but after adjustment for tobacco smoking, alcohol drinking and betel quid chewing the association was no longer apparent. The adjusted OR were 1.10 (95% CI=0.80-1.50) for family history of tobacco-related cancer and 0.96 (95%CI=0.75-1.24) for family history of any cancer with adjustment for tobacco, betel quid and alcohol habits. The ORs for having a first-degree relative with HNC were higher in all tobacco/alcohol subgroups. Conclusions: We did not observe a strong association between family history of head and neck cancer and head and neck cancer risk after taking into account lifestyle factors. Our study suggests that an increased risk due to family history of head and neck cancer may be due to shared risk factors. Further studies may be needed to assess the lifestyle factors of the relatives.

A three-dimensional in vitro culture model for primary neonatal rat ventricular myocytes

Hongxia Zheng,Shanshan Liu,Wei-ming Tian,Hong-ji Yan,Yao Zhang,Yu Li 한국물리학회 2012 Current Applied Physics Vol.12 No.3

To overcome bad prognosis of patients with heart failure and the lack of organ donors, cardiac tissue engineering has developed as a biomimetic approach to repair, replace, and regenerate the damaged cardiac tissue. During the past decade years, researchers are devoted to find different natural and/or synthetic materials that can build appropriate physical structures to contain and organize implanted cells. In this study, we present a new method for primary neonatal rat cardiomyocytes culture in vitro using alginate/collagen/chitosan hydrogel. To investigate the feasibility of this material as scaffold for cardiac myocytes, neonatal rat ventricular myocytes were isolated and encapsulated in alginate-based beads cross-linked with calcium ion. The growth of cells was evaluated by staining with a-Sarcomeric actin (a-SCA) and Troponin T type 2 (TNNT2), and the viability of cardiomyocytes was studied in vitro by assessing the expression levels of several cardiac ion channels, including CACNL1A1, Connexin 43 and SCN5A. The results showed a significant increase in cardiac myocytes number, and the expression levels of CACNL1A1, Connexin 43 (Cx43) were up-regulated significantly except SCN5A, as compared with twodimensional cultures. Moreover, extracellular matrix produced by the seeded cells themselves was observed by staining with fibronectin. Taken together, these findings indicate that this alginate/collagen/chitosan hydrogel bead is suitable for supporting the growth and retaining the morphologic and electrophysiologic characteristics of primary cultured rat cardiac muscle cells.

The Feasibility of Using Biomarkers Derived from Circulating Tumor DNA Sequencing as Predictive Classifiers in Patients with Small-Cell Lung Cancer

Yu Feng,Yutao Liu,Mingming Yuan,Guilan Dong,Hongxia Zhang,Tongmei Zhang,Lianpeng Chang,Xuefeng Xia,Lifeng Li,Haohua Zhu,Puyuan Xing,Hongyu Wang,Yuankai Shi,Zhijie Wang,Xingsheng Hu 대한암학회 2022 Cancer Research and Treatment Vol.54 No.3

Purpose To investigate the feasibility of biomarkers based on dynamic circulating tumor DNA (ctDNA) to classify small cell lung cancer (SCLC) into different subtypes. Materials and Methods Tumor and longitudinal plasma ctDNA samples were analyzed by next-generation sequencing of 1,021 genes. PyClone was used to infer the molecular tumor burden index (mTBI). Pre-treatment tumor tissues [T1] and serial plasma samples were collected (pre-treatment [B1], after two [B2], six [B3] cycles of chemotherapy and at progression [B4]). Results Overall concordance between T1 and B1 sequencing (n=30) was 66.5%, and 89.5% in the gene of <i>RB1</i>. A classification method was designed according to the changes of <i>RB1</i> mutation, named as subtype Ⅰ (both positive at B1 and B2), subtype Ⅱ (positive at B1 but negative at B2), and subtype Ⅲ (both negative at B1 and B2). The median progressive-free survival for subtype Ⅰ patients (4.5 months [95%CI: 2.6-5.8]) was inferior to subtype Ⅱ (not reached, p<0.0001) and subtype Ⅲ (10.8 months [95%CI: 6.0-14.4], p=0.002). The median overall survival for subtype Ⅰ patients (16.3 months [95%CI: 5.3-22.9]) was inferior to subtype Ⅱ (not reached, p=0.01) and subtype Ⅲ (not reached, p=0.02). Patients with a mTBI dropped to zero at B2 had longer median overall survival (not reached vs. 19.5 months, p=0.01). The changes of mTBI from B4 to B1 were sensitive to predict new metastases, with a sensitivity of 100% and a specificity of 85.7%. Conclusion Monitoring ctDNA based <i>RB1</i> mutation and mTBI provided a feasible tool to predict the prognosis of SCLC.

Environmental Issues on End-of-Life Battery from Next-Generation Vehicles in Japan

( Jeongsoo Yu ),( Xiaoyue Liu ),( Hongxia Chen ) 한국폐기물자원순환학회 2022 ISSE 초록집 Vol.2022 No.-

Manufacture and recycling of vehicle batteries in China, Japan and Korea have drawn more and more attention, the types of batteries in different countries are not uniform, so it is difficult to ensure the recovery of rare metals. To meet the increasing manufacturing demand of Next-Generation Vehicles (NGVs), the importance of reuse and recycling of vehicle batteries is also increasing. This research gives an overview of the popularization of NGVs, as well as the status and topics of reuse and recycling of vehicle batteries in Japan. Japanese government and auto makers have already established a battery recycling system; however, NiMH batteries and LIBs are usually exported along with massive secondhand vehicles. In NiMH batteries LCA case, the reuse and/or recycling of EoL NiMH batteries will bring more environmental burdens than the nonrecycling scenario. However, by reusing or recycling EoL NiMH batteries, we can avoid manufacturing new NiMH batteries or recycle certain metal resources. The reuse policy for EoL NiMH batteries should be implemented and the EoL NiMH batteries treated, as soon as possible in Japan. In addition, there appear to be a vast number of low-capacity waste NiMH battery exports from Japan to developing countries. These batteries will lose their function completely soon after being exported and may eventually be inappropriately treated.

A Novel Control Method for Drum Level and Pressure Basing on Dynamic Surface

Yuanwei Jing,Hongxia Yu,Xiaoyu Sun 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.6

A novel drum level and pressure simultaneously control method is developed using the dynamic surface control method in this paper which takes account of the output power of units and load change .Certain first-order low-pass filters are introduced into the designing process to avoid the occurrence of high-order derivatives of elements in the system which makes it easy to implement in practical applications. The proposed control method is effective in compensating for the disturbance of load and fuel. Simulation results show that the dynamic surface control method still ensures an accurate result, even if the loads change in a great and parameters of the controlled plant change significantly.

Carnosic acid protects against acetaminophen-induced hepatotoxicity by potentiating Nrf2-mediated antioxidant capacity in mice

Qi Guo,Zhiyang Shen,Hongxia Yu,Gaofeng Lu,Yong Yu,Xia Liu,Pengyuan Zheng 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Acetaminophen (APAP) overdose is one of the most common causes of acute liver failure. The study aimed to investigate the protective effect of carnosic acid (CA) on APAP-induced acute hepatotoxicity and its underlying mechanism in mice. To induce hepatotoxicity, APAP solution (400 mg/kg) was administered into mice by intraperitoneal injection. Histological analysis revealed that CA treatment significantly ameliorated APAP-induced hepatic necrosis. The levels of both alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum were reduced by CA treatment. Moreover, CA treatment significantly inhibited APAP-induced hepatocytes necrosis and lactate dehydrogenase (LDH) releasing. Western blot analysis showed that CA abrogated APAP-induced cleaved caspase-3, Bax and phosphorylated JNK protein expression. Further results showed that CA treatment markedly inhibited APAP-induced pro-inflammatory cytokines TNF-a, IL-1b, IL-6 and MCP-1 mRNA expression and the levels of phosphorylated IkBa and p65 protein in the liver. In addition, CA treatment reduced APAP- induced hepatic malondialdehyde (MDA) contents and reactive oxygen species (ROS) accumulation. Conversely, hepatic glutathione (GSH) level was increased by administration of CA in APAP-treated mice. Mechanistically, CA facilitated Nrf2 translocation into nuclear through blocking the interaction between Nrf2 and Keap1, which, in turn, upregulated anti-oxidant genes mRNA expression. Taken together, our results indicate that CA facilitates Nrf2 nuclear translocation, causing induction of Nrf2-dependent genes, which contributes to protection from acetaminophen hepatotoxicity.

Fusion Detection Algorithm for Infrared Dim Target based on Temporal-Spatial Domain Accumulation and Difference

Jianfeng Wu,Shucai Huang,Hongxia Kang,Yu Zhong 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.11

Aiming at the problem of the dim target real-time detection in Infrared search and track (IRST) systems, a fusion detection algorithm for infrared dim target based on temporal-spatial domain accumulation and difference is proposed. The proposed algorithm suppresses background and enhances targets by template filtering and difference processing in space domain, and the image sequences are processed by multi-frame energy accumulation and frame difference methods according to the target’s moving characteristics in time domain. After the fusion segmentation of the temporal and spatial processing results with definite rule, the target can be detected by the relation of the possible target’s position in adjoining frames according to target’s moving continuity and regularity. Experimental results show that the proposed algorithm enormously increases the target's SNR after the temporal-spatial fusion enhancement, and it has high detection probability and high detecting speed. In the meantime, the algorithm is easy to realize in hardware and can be applied effectively in the real-time target detection of the IRST systems.

Carnosic acid protects against acetaminophen-induced hepatotoxicity by potentiating Nrf2-mediated antioxidant capacity in mice

Guo, Qi,Shen, Zhiyang,Yu, Hongxia,Lu, Gaofeng,Yu, Yong,Liu, Xia,Zheng, Pengyuan The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Acetaminophen (APAP) overdose is one of the most common causes of acute liver failure. The study aimed to investigate the protective effect of carnosic acid (CA) on APAP-induced acute hepatotoxicity and its underlying mechanism in mice. To induce hepatotoxicity, APAP solution (400 mg/kg) was administered into mice by intraperitoneal injection. Histological analysis revealed that CA treatment significantly ameliorated APAP-induced hepatic necrosis. The levels of both alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum were reduced by CA treatment. Moreover, CA treatment significantly inhibited APAP-induced hepatocytes necrosis and lactate dehydrogenase (LDH) releasing. Western blot analysis showed that CA abrogated APAP-induced cleaved caspase-3, Bax and phosphorylated JNK protein expression. Further results showed that CA treatment markedly inhibited APAP-induced pro-inflammatory cytokines TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and MCP-1 mRNA expression and the levels of phosphorylated $I{\kappa}B{\alpha}$ and p65 protein in the liver. In addition, CA treatment reduced APAP- induced hepatic malondialdehyde (MDA) contents and reactive oxygen species (ROS) accumulation. Conversely, hepatic glutathione (GSH) level was increased by administration of CA in APAP-treated mice. Mechanistically, CA facilitated Nrf2 translocation into nuclear through blocking the interaction between Nrf2 and Keap1, which, in turn, upregulated anti-oxidant genes mRNA expression. Taken together, our results indicate that CA facilitates Nrf2 nuclear translocation, causing induction of Nrf2-dependent genes, which contributes to protection from acetaminophen hepatotoxicity.

Long non-coding RNA TTC28-AS1 attenuates high glucose-induced damage in HK-2 cells depending on the regulation of miR-320a/CD2AP axis

Zhang Ji,Ding Juan,Yu Ming,Li Fang,Zhou Xue,Shuai Hongxia 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Diabetic nephropathy (DN) is the leading cause of end-stage renal disease (ESRD) worldwide. Emerging evidence suggests that long non-coding RNAs (lncRNAs) play crucial roles in DN pathogenesis. Objective The purpose of the present study was to explore the role and mechanism of lncRNA tetratricopeptide repeat domain 2B antisense RNA 1 (TTC28-AS1) in DN. Methods Cell viability and apoptosis were assessed by the Cell Counting-8 Kit (CCK-8) assay and fow cytometry, respectively. The levels of TTC28-AS1, miR-320a and CD2-associated protein (CD2AP) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNFα), and IL-8 were gauged by enzyme-linked immunosorbent assay (ELISA). Targeted relationship between miR-320a and TTC28-AS1 or CD2AP was evaluated by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Results Our data indicated that high glucose (HG) induced HK-2 cell damage by the repression of cell viability and autophagy and the enhancement of cell apoptosis, fbrosis and pro-infammatory cytokines production. TTC28-AS1 was down-regulated and miR-320a was up-regulated in HG-induced HK-2 cells. TTC28-AS1 overexpression or miR-320a knockdown alleviated HG-induced damage in HK-2 cells. MiR-320 was a molecular mediator of TTC28-AS1 in regulating HG-induced HK-2 cell damage. Moreover, TTC28-AS1 functioned as a post-transcriptional regulator of CD2AP expression by miR-320a. MiR-320a knockdown relieved HG-induced damage in HK-2 cells by up-regulating CD2AP. Conclusions Our fndings suggest that TTC28-AS1 attenuates HG-induced damage in HK-2 cells at least partially by targeting the miR-320a/CD2AP axis, highlighting its role as a promising therapeutic approach for DN treatment.

SPWM Implementation Using DSP Model Programming Based on Simulink

ChangyongY in,Xiaoyu Sun,Shuo Huang,Hongxia Yu 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.8

SPWM is an essential modulation algorithm in power electronic devices, but it is not easy for unskilled programmer or junior engineers to build the code effectively. Model programming with embedded target supporting package in Matlab/Simulink is a new method to replace the traditional code writing without complicacy. Comparing to the manual method, it is more effective with lower cost, and the most importantly, it is more reliable. This paper offers a popular asymmetric sampling SPWM prototype with algorithm deduction, codes and models from Matlab RTW-EC. Finally, a practical result which can prove the method to be functional is also provided. This may accelerate the power electronics developing project with reliability in projects.