배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

A composite crack model for concrete based on meshless method

Lu, Xin-Zheng,Jiang, Jian-Jing,Ye, Lie-Ping Techno-Press 2006 Structural Engineering and Mechanics, An Int'l Jou Vol.23 No.3

A crack model for the fracture in concrete based on meshless method is proposed in this paper. The cracks in concrete are classified into micro-cracks or macro-cracks respectively according to their widths, and different numerical approaches are adopted for them. The micro-cracks are represented with smeared crack approach whilst the macro-cracks are represented with discrete cracks that are made up with additional nodes and boundaries. The widely used meshless method, Element-free Galerkin method, is adopted instead of finite element method to model the concrete, so that the discrete crack approach is easier to be implemented with the convenience of arranging node distribution in the meshless method. Rotating-Crack-Model is proved to be preferred over Fixed-Crack-Model for the smeared cracks of this composite crack model due to its better performance on mesh bias. Numerical examples show that this composite crack model can take advantage of the positive characteristics in the smeared and discrete approaches, and overcome some of their disadvantages.

Integrating Granger Causality and Vector Auto-Regression for Traffic Prediction of Large-Scale WLANs

( Zheng Lu ),( Chen Zhou ),( Jing Wu ),( Hao Jiang ),( Songyue Cui ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.1

Flexible large-scale WLANs are now widely deployed in crowded and highly mobile places such as campus, airport, shopping mall and company etc. But network management is hard for large-scale WLANs due to highly uneven interference and throughput among links. So the traffic is difficult to predict accurately. In the paper, through analysis of traffic in two real large-scale WLANs, Granger Causality is found in both scenarios. In combination with information entropy, it shows that the traffic prediction of target AP considering Granger Causality can be more predictable than that utilizing target AP alone, or that of considering irrelevant APs. So We develops new method - Granger Causality and Vector Auto-Regression (GCVAR), which takes APs series sharing Granger Causality based on Vector Auto-regression (VAR) into account, to predict the traffic flow in two real scenarios, thus redundant and noise introduced by multivariate time series could be removed. Experiments show that GCVAR is much more effective compared to that of traditional univariate time series (e.g. ARIMA, WARIMA). In particular, GCVAR consumes two orders of magnitude less than that caused by ARIMA/WARIMA.

Investigation on Psychological Distress of the Dialytic Patients with End-stage Failure

Lu-Zheng,Ji Jiang-Ling,Zhou An-Qi,Xu jun-Mian 대한스트레스학회 1998 스트레스硏究 Vol.6 No.2

An experimental study of vibration control of wind-excited high-rise buildings using particle tuned mass dampers

Zheng Lu,Dianchao Wang,Sami F. Masri,Xilin Lu 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.18 No.1

A particle tuned mass damper (PTMD) system is the combination of a traditional tuned mass damper (TMD) and a particle damper (PD). This paper presents the results of an experimental and analytical study of the damping performance of a PTMD attached to the top of a benchmark model under wind load excitation. The length ratio of the test model is 1:200. The vibration reduction laws of the system were explored by changing some system parameters (including the particle material, total auxiliary mass ratio, the mass ratio between container and particles, the suspending length, and wind velocity). An appropriate analytical solution based on the concept of an equivalent single-unit impact damper is presented. Comparison between the experimental and analytical results shows that, with the proper use of the equivalent method, reasonably accurate estimates of the dynamic response of a primary system under wind load excitation can be obtained. The experimental and simulation results show the robustness of the new damper and indicate that the damping performance can be improved by controlling the particle density, increasing the amount of particles, and aggravating the impact of particles etc.

Over-expression of Chinese cabbage calreticulin 1, BrCRT1, enhances shoot and root regeneration, but retards plant growth in transgenic tobacco

Zheng-Lu Jin,Hong, Joon-Ki,Yang, Kyung-Ae,Koo, Ja-Choon,Choi, Young-Ju,Chung, Woo-Sik,Yun, Dae-Jin,Lee, Sang-Yeol,Cho, Moo-Je,Lim, Chae-Oh Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Calreticulin (CRT) is a ubiquitously expressed, high capacity Ca^(2+)-binding protein that is involved in intracellular Ca^(2+) homeostasis and molecular chaperoning in the endoplasmic reticulum (ER). A cDNA encoding a calreticulin, BrCRT1 (Brassica rapa Calreticulin 1), has been isolated from Chinese cabbage (B. rapa subsp. pekinensis) flower bud. Constitutive over-expression of the BrCRT1 gene promotes robust shoot production and root formation at sub-optimal concentrations of BA/NAA, which are important factors controlling plant regeneration in tissue culture. In contrast, the suppressed BrCRT1 line exhibited a slight reduction of shoot and root regeneration. In spite of enhanced regeneration in tissue culture, the seedling and plant growth rate was inhibited in soil. The steady state level of BrCRT1 transcripts was sensitive to exogenous auxins and cytokinins, and rapidly accumulated within 30 min, and this induction required de novo protein synthesis. Together with the results of transgenic tobacco plants and mRNA analysis in Chinese cabbage, our data suggest that BrCRT1 genes may up-regulate the competency of vegetative tissue to respond to hormonal signals involved in shoot and root regeneration processes.

Optimization of Medium Components for Plasmid Production by Recombinant E. coli $DH5{\alpha}$ $pUK21CMV{\beta}1.2$

Zheng, Shuzhen,Friehs, Karl,He, Ning,Deng, Xu,Li, Qingbiao,He, Zheng,Xu, Chenyun,Lu, Yinghua Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3

In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by $pUK21CMV{\beta}1.2$ harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80mg/L and a plasmid selectivity of 23.49mg/g were achieved after 24h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.

Vibration control performance of particle tuned mass inerter system

Zheng Lu,Deyu Yan,Chaojie Zhou,Ruifu Zhang 국제구조공학회 2024 Structural Engineering and Mechanics, An Int'l Jou Vol.89 No.4

To improve the vibration control performance and applicability of traditional particle tuned mass damper (PTMD) and realize the significant characteristic of lightweight design, this study proposes a novel particle tuned mass inerter system (PTMIS) by introducing inerter system (IS) to the PTMD. In the study, the motion equation of single degree of freedom (SDOF) structure attached with PTMIS is established first, then the variation law of the system’s vibration reduction performance (VRP) is discussed through parameter analysis, and it is compared with the PTMD to analyze its VRP advantages. Finally, its vibration reduction (VR) mechanism from the perspective of core control force and energy analysis is explored, and its cavity relative displacement from the application perspective is analyzed. The results show that the PTMIS can remarkably improve the vibration control effectiveness of the PTMD. The reason is that the inerter can store energy and transfer the energy to the cavity and particles, which further stimulates the interaction between the two parts, thereby improving the nonlinear energy consumption effectiveness. Also, the IS can amplify the damping element’s energy dissipation efficiency. In addition, the PTMIS can effectively reduce the working stroke of the PTMD, and through the analysis of the lightweight characteristics of the PTMIS, it is found that its lightweight advantage can reach nearly 100%.

Optimization and application of multiple tuned mass dampers in the vibration control of pedestrian bridges

Zheng Lu,Xiaoyi Chen,Xiaowei Li,Peizhen Li 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.62 No.1

An effective design approach for Multiple Tuned Mass Dampers (MTMDs) in pedestrian bridges was proposed by utilizing the transfer function to obtain each TMD’s optimum stiffness and damping. A systematic simulation of pedestrian excitations was described. The motion equation of a typical MTMD system attached to a Multi-degree-of-freedom (MDOF) system was presented, and the transfer function from the input pedestrian excitations to the output acceleration responses was defined. By solving the minimum norm of the transfer function, the parameters of the MTMD which resulted in the minimum overall responses can be obtained. Two applications of lightly damped pedestrian bridges attached with MTMD showed that MTMDs designed through this method can significantly reduce the structural responses when subjected to pedestrian excitations, and the vibration control effects were better than the MTMD when it was considered as being composed of equal number and mass ratios of TMDs designed by classical Den Hartog method.

The Impact of Brand Experience on User Satisfaction in Mobile Short Video Applications

Lu Zheng,김영길(Yeong-Gil Kim) 한국무역연구원 2021 무역연구 Vol.17 No.4

Purpose - How to successfully retain short video users and enhance the market competitiveness of short video companies has become an urgent problem in the short video industry. Design/Methodology/Approach - This study selects users on mobile short video platforms as the research object, conducts research from the new perspective and field of brand experience of short video applications, this study adopts the method of combining qualitative and quantitative measurement., studies the relationship between brand experience, perceived value, brand personality and satisfaction, tests the mediating role of perceived value, and explains the role of brand personality in the process of brand experience’s influence on user satisfaction. Findings - Emotional experience, thinking experience, and behavioral experience all positively affect perceived value; Perceived value positively affects user satisfaction; Brand experience positively affects user satisfaction; Perceived value plays an intermediary role in the relationship between emotional experience, thinking experience, and behavioral experience on user satisfaction; Brand personality plays a positive moderating role in the influence of sensory experience, thinking experience, behavioral experience and associative experience on user satisfaction. Research Implications - The theoretical development of brand experience is expanded to provide reference for short video industry to formulate corresponding marketing strategies for different customer groups