소독음(消毒飮)에탄올 추출물이 사람각질형성세포 HaCaT 세포주의 자외선 손상에 미치는 영향

전규상 ( Kyu Sang Jun ),김일현 ( Il Hyun Kim ),임광묵 ( Gwang Mook Lim ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2012 한방재활의학과학회지 Vol.22 No.3

Objectives :Sodok-eum(xiaodu-yˇln, SDU) exhibits potent anti-inflammatory activity with an unknown mechanism. The purpose of this study is to elucidate the molecular mechanisms of SDU on pharmacological and biochemical actions in inflammation. Methods :We examined the effect of SDU on pro-inflammatory mediators in lipopolysaccharide(UVB)-stimulated HaCaT. The investigation focused on whether SDU inhibited cyclooxygenase-2(COX-2), interleukin-6(IL-6), interleukin-8(IL-8) and mitogen-activated protein kinases(MAPKs) in UVB-stimulated HaCaT cells. Results :SDU inhibited UVB-induced IL-6, IL-8 productions as well as the expressions of COX-2. Furthermore, SDU suppressed the UVB-induced phosphorylation of extracellular signal-regulated kinase 1/2(ERK1/2), c-JUN N-terminal kinase 1/2(JNK 1/2), p38. Conclusions :These results suggest that SDU has inhibitory effects on UVB-induced IL-6, IL-8 production, as well as the expressions of COX-2 in the HaCaT. The inhibitory effects occur through blockades on the phosphorylation of MAPKs following activation.

아토피 피부염 모델에 대한 β-1,3/1,6-glucan과 Lactobacillus plantarum LM1004의 면역 조절 효과

김인성(In Sung Kim),김성학(Sung Hak Kim),김정아(Jeong A Kim),유다윤(Da Yoon Yu),김광일(Gwang Il Kim),박동찬(Dong-Chan Park),임종민(Jong Min Lim),이상석(Sang Suk Lee),최인순(In Soon Choi),조광근(Kwang Keun Cho) 한국생명과학회 2018 생명과학회지 Vol.28 No.1

본 연구에서는 아토피 피부염 동물 모델에 대한 β-1,3/1,6-glucan과 L. plantarum LM1004의 면역조절 효과를 확인하고자 하였다. 가려움증의 횟수와 유출된 evans blue, 그리고 혈청 IgE와 histamine의 농도는 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹에서 아토피 피부염 유발그룹에 비해 유의적으로 감소하는 결과를 나타내었다. 아토피 피부염이 유발되면 전사 수준에서 Th2 및 Th17 세포의 전사인자 및 cytokine은 과발현되며, β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취하였을 때 이를 유의적으로 감소되었다. 또한 β-1,3/1,6-glucan과 L. plantarum LM1004는 Th1 및 Treg 세포의 전사인자(T-bet, GATA-3, RORγT, Foxp3) 및 cytokine (INF-γ, IL-4, IL-17, TGF-β)의 발현을 증가시킴으로써 면역 균형을 조절하는 것으로 나타났다. Galectin-9과 filaggrin은 아토피 피부염 유발 처리군에서 유의적으로 가장 낮았으며, β-1,3/1,6-glucan 처리군에서 유의적으로 가장 높게 나타났다. 이와 반대로 TSLP는 아토피 피부염 유발그룹에서 유의적으로 가장 높았으며 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹은 대조군과 유사한 수준이었다. 이러한 결과를 통해 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염 동물 모델에서 면역조절 작용 및 아토피 피부염의 개선 효과를 가짐을 알 수 있었다. 따라서 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염에 유용한 천연소재로서 사용될 것으로 기대된다. In this study, we examined the efficacy of the immune regulation of β-1,3/1,6-glucan and Lactobacillus plantarum LM1004 on atopic dermatitis models. The oral administration of β-1,3/1,6-glucan and L. plantarum LM1004 on mice significantly decreased the amount of scratching, leakage to evans blue, and concentrations of serum immunoglobulin E (IgE) and histamine compared with the atopic dermatitis–induced group. When atopic dermatitis was induced, the transcription factors (GATA-3, retinoic acid-related orphan receptor γ T [RORγT]) and cytokines (interleukin-4 [IL-4], IL-17) of Th2 and Th17 cells were overexpressed at the transcriptional level, and they significantly decreased with oral administration of β-1,3/1,6-glucan and L. plantarum LM1004. In addition, β-1,3/1,6-glucan and L. plantarum LM1004 were shown to modulate the immune balance by increasing the expression of Th1 and Treg transcription (T-bet, forkhead box p3 [Foxp3]) and cytokines (interferon-γ [IFN-γ], transforming growth factor-β [TGF-β]). Galectin-9 and filaggrin were significantly lower in the atopic dermatitis–induced group and significantly higher in the β-1,3/1,6-glucan-treated group. In contrast, thymic stromal lymphopoietin (TSLP) was highest in the atopic dermatitis–induced group, while mice that were orally administered β-1,3/1,6-glucan and L. plantarum LM1004 showed similar TSLP levels to the control group. These results indicate that β-1,3/1,6-glucan and L. plantarum LM1004 have immunomodulatory effects and atopic dermatitis improvement effects in an animal model of atopic dermatitis. Therefore, it is expected that β-1,3/1,6-glucan and L. plantarum LM1004 can be used as natural materials in the treatment of atopic dermatitis.

Rapid Determining for Subtypes and Pandemic Type of Swine Influenza Virus by Diagnostic One-step RT-PCR

Gwang Il Kim(김광일),Jee In Kim(김지인),Jin-Hyeap Kwon(권진협),Yoo Hong Min(민유홍),Joo Il Kang(강주일),Chang-Ho Lee(이창호),Sung-Hee Kim(김성희),Jae-Hwan Lim(임재환) 한국생명과학회 2018 생명과학회지 Vol.28 No.5

Swine influenza virus (SIV)는 돼지 개체군에서 가장 흔한 질병을 일으키는 바이러스 중 하나이며 그 subtype은 hemagglutinin (HA)와 neuraminidase (NA)에 의해 결정됩니다. 최근 SIV subtype 진단 방법이 개발되고 있으나 SIV의 리보뉴클레오타이드 서열의 많은 변이로 인해 PCR 보다는 항원-항체 반응을 이용하는 방법이 주로 사용되고 있다. 본 연구에서는 SIV 하위 유형의 신속한 결정을 위하여 2008년 이후 국내에서 발생한 SIV의 다중염기서열 정렬을 통하여 10개의 subtype 진단 프라이머 세트를 개발하고 이를 이용한 one-step RT-PCR 반응을 최적화하였다. 또한 감염력이 높고 독성이 있는 인플루엔자 H1N1 (pH1N1)의 아형에서 확인된 독특한 M 유전자 서열을 검출함으로써 pandemic SIV를 조기에 결정하도록 특이적 프라이머를 설계하였다. 2008년부터 2014년까지 한국에서 발생한 9종의 SIV RNA를 활용하여 SIV의 아형 및 pandemic 가능성을 결정하기 위해 시험 분석한 결과 모든 진단 프라이머 세트는 SIV 아형을 정확하게 결정하였으며 pandemic SIV를 검출할 수 있는 것으로 확인되었다. 결과적으로 이들 프라이머 세트를 이용한 최적화된 one-step RT-PCR 분석이 SIV 아형의 신속한 진단에 유용하다는 것이 확인하였다. 이러한 결과는 SIV 하위 유형 및 pandemic SIV가 확산되기 전에 조기 발견을 위한 키트로 개발될 수 있음을 시사한다. Swine influenza virus (SIV) causes one of the most common diseases of the pig population, and its subtypes are determined by hemagglutinin (HA) and neuraminidase (NA). Recently, the SIV subtype diagnosis has been developed. The method using antigen-antibody reaction rather than PCR was mainly used because of the large change in the ribonucleotide sequences of SIV. Here, we have developed 10 diagnostic primer sets through multi-nucleotide sequences alignment of spreaded SIV since 2008 in Korea and then optimized the reaction of the one-step RT-PCR for rapid determination of SIV subtype. In addition, specific primers were designed to early determine the pandemic SIV by detecting unique M sequences proven in highly infectious and virulent subtypes of the influenza H1N1 (pH1N1). Here, some of the SIVs spread in Korea from 2008 to 2014 have been tested to determine the subtypes and pandemic potential of SIV. All diagnostic primer sets were found to be able to accurately determine the SIV subtype and to detect the pandemic SIV. In conclusion, it was confirmed that the optimized one-step RT-PCR analysis using these primer sets is useful for rapid diagnosis of SIV subtypes. These results can be used for development of SIV subtype diagnostic kit to early detect before virulent SIV spreads do.

Influence of Bradykinin on Catecholamine Release from the Rat Adrenal Medulla

Lim, Dong-Yoon,Kim, Il-Hwan,Na, Gwang-Moon,Kang, Moo-Jin,Kim, Ok-Min,Choi, Deok-Ho,Ki, Young-Woo The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.4

The present study was undertaken to investigate the effect of bradykinin on secretion of catecholamines (CA) evoked by stimulation of cholinergic receptors and membrane depolarization from the isolated perfused model of the rat adrenal glands, and to elucidate its mechanism of action. Bradykinin $(3{\times}10^{-8}M)$ alone produced a weak secretory response of the CA. however, the perfusion with bradykinin $(3{\times}10^{-8}M)$ into an adrenal vein of the rat adrenal gland for 90 min enhanced markedly the secretory responses of CA evoked by ACh $(5.32{\times}10^{-3}M)$, excess $K^+$ ($5.6{\times}10^{-2}M$, a membrane depolarizer), DMPP ($10^{-4}$ M, a selective neuronal nicotinic agonist) and McN-A-343 ($10^{-4}$ M, a selective M1-muscarinic agonist). Moreover, bradykinin ($3{\times}10^{-8}$ M) in to an adrenal vein for 90 min also augmented the CA release evoked by BAY-K-8644, an activator of the dihydropyridine L-type $Ca^{2+}$ channels. However, in the presence of $(N-Methyl-D-Phe^7)$-bradykinin trifluoroacetate salt $(3{\times}10^{-8}M)$, an antagonist of $BK_2$-bradykinin receptor, bradykinin no longer enhanced the CA secretion evoked by Ach and high potassium whereas the pretreatment with Lys-$(des-Arg^9,\;Leu^9)$-bradykinin trifluoroacetate salt $(3{\times}10^{-8}M)$, an antagonist of $BK_1$-bradykinin receptor did fail to affect them. Furthermore, the perfusion with bradykinin $(3{\times}10^{-6}M)$ into an adrenal vein of the rabbit adrenal gland for 90 min enhanced markedly the secretory responses of CA evoked by excess $K^+$ $(5.6{\times}10^{-2}M)$. Collectively, these experimental results suggest that bradykinin enhances the CA secretion from the rat adrenal medulla evoked by cholinergic stimulation (both nicotininc and muscarinic receptors) and membrane depolarization through the activation of $B_2$-bradykinin receptors, not through $B_1$-bradykinin receptors. This facilitatory effect of bradykinin seems to be associated to the increased $Ca^{2+}$ influx through the activation of the dihydropyridine L-type $Ca^{2+}$ channels.

Amorphous Tin Oxide Nanohelix Structure Based Electrode for Highly Reversible Na-Ion Batteries

Choi, Il Yong,Jo, Changshin,Lim, Won-Gwang,Han, Jong-Chan,Chae, Byeong-Gyu,Park, Chan Gyung,Lee, Jinwoo,Kim, Jong Kyu American Chemical Society 2019 ACS NANO Vol.13 No.6

<P>An array of amorphous tin oxide (a-SnO<SUB>x</SUB>) nanohelixes (NHs) was fabricated on copper foil as an electrode for Na-ion batteries <I>via</I> the oblique angle deposition method, a solution- and surfactant-free process. The combination of the amorphous phase SnO<SUB>x</SUB> with a low oxidation number and its vertically aligned NH geometry with a large surface area and high porosity, which facilitate Na-ion dynamics and accommodate the volume changes, enabled a reversible capacity of up to 915 mA h g<SUP>?1</SUP> after 50 cycles, fast rate capability with 48.1% retention at 2 A g<SUP>?1</SUP>, and high stability, which are superior to those of crystalline nanoparticle-based electrodes.</P> [FIG OMISSION]</BR>

Reappraisal of the Accuracy of Diagnostic Criteria for Spontaneous Bacterial Peritonitis in Cirrhotic Patients with or without Hepatocellular Carcinoma: A Preliminary Result

( Ha Il Kim ),( Ju Hyun Shim ),( Gwang Hyeon Choi ),( Danbi Lee ),( Kang Mo Kim ),( Young-suk Lim ),( Han Chu Lee ),( Young-hwa Chung ),( Yung Sang Lee ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: We aimed to reevaluate whether the traditional cutoff count of polymorphonuclear-leucocyte(PMN,≥250/mm3) would be still optimal to diagnose spontaneous bacterial peritonitis(SBP) in cirrhotics having a hepatocellular carcinoma(HCC) or not. Methods: This preliminary study included 136 consecutive patients having cirrhosis with(n=60) or without HCC(n=76) and a positive ascitic-fluid(AF) culture at the first exploratory paracentesis between 2007 and 2016 as the SBP group; and 202 cirrhotics with neither bacterascites on the first AF specimen nor any typical symptoms suggestive of peritoneal infection as the non-SBP control group. Among the SBP group, none had clinical suspicion of secondary or tertiary peritonitis and malignant ascites, and history of non-HCC cancers. Results: Fever(28%) and E. coli(22%) were the most common symptom and isolated pathogen, respectively in the SBP group. There was no patient with ascitic-PMN≥250/mm3 in the control group. Ascitic white-blood-cell and PMN counts, and lactate-dehydrogenase were significantly higher in the SBP group, whereas glucose and serum-ascites-albumin-gradient were higher in the control group Ps<0.05). Total-protein and pH in the AF were similar in both groups. For the entire SBP patients, receiver-operating-characteristic analysis to determine the diagnostic performance of ascitic-PMN≥250/mm3 showed a sensitivity of 87% and a specificity of 95%, comparably between the LC and HCC subsets. There were no differences in both AF results and symptoms between SBP patients with cirrhosis and HCC. The 90th-percentile limits for the ascitic-PMN counts were 246 and 466/mm3, respectively for the LC and HCC subsets with SBP. The number of SBP patients with ascitic-PMN<250/ mm3 was 11(8%), consisting of 6 cirrhosis and 5 HCCs. Conclusions: Based on our results, an update or revision of the old threshold count for ascetic-PMN may be taken into consideration to be timely treated with antibiotics in not only HCC patients but cirrhotics who have established or suspected SBP, leading to prevention of undertreatment of serious infection and overtreatment of sterile ascites.

Concentration of Tumor Necrosis Factor-alpha (TNF-α) in Induced Sputum and TNF-α Gene Polymorphism in COPD

( Yu Il Kim ),( Jong Pil Jeong ),( Jun Gwang Son ),( Soo Ok Kim ),( In Jae Oh ),( Mi Ran Kim ),( Ju Yeon Jeong ),( Kyu Sik Kim ),( Sung Chul Lim ),( Young Chul Kim ),( Kyung Ok Park ) 대한결핵 및 호흡기학회 2006 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.103 No.-

Possible Role of Surgery in the Treatment of Patients with Intermediate Stage Hepatocellular Carcinoma and Preserved Hepatic Function

( Ha Il Kim ),( Jihyun An ),( Ju Hyun Shim ),( Seheon Chang ),( Sangyoung Yi ),( Jonggi Choi ),( Gwang Hyeon Choi ),( Danbi Lee ),( Kang Mo Kim ),( Young-suk Lim ),( Han Chu Lee ),( Young-hwa Chung ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Transarterial chemoembolization (TACE) is a current standard therapy based on scientific evidences for patients with intermediate stage hepatocellular carcinoma (HCC). A large number of these patients are usually incurable by TACE alone, and moreover repetition of TACE can increase the risk of liver deterioration. Multiplicity of lesions is not a contraindication for radical hepatectomy, although it is a potential risk factor of recurrence. Based on these backgrounds, we aimed to identify survival benefits of surgical resection compared with TACE, a standard-of-care, for patients with intermediate HCC. Methods: A total of 548 patients with BCLC B stage HCC and Child-Pugh A hepatic function underwent surgical resection (n=163) or TACE (n=385) as a primary therapy between 2007 and 2011 at our hospital. Their median age was 57 years (range 51-65), and most of them were male (85.9%) and positive for HBV (73.7%). All-cause and cancer-specific mortalities were measured and compared in the two groups (i.e, surgery and TACE). Results: The mean number of tumors (2.3±0.8 vs. 3.4±1.5) and the proportion of portal hypertension (8.6% vs. 26.0%) were greater in the TACE group than in the surgery group (Ps<0.05). During the median follow-up of 3.6 years, resection provided a survival benefit over TACE at 5 years (63.2% vs. 32.2%; P<0.05). Cancer-specific mortality rate at 5 years was also significantly lower in the surgery group (9.6% vs. 36.0%; P<0.05). In the adjusted Cox model, resection was identified as an independent predictor of better all-cause and cancer-specific mortality outcomes (adjusted HRs [95% CI], 0.46 [0.36-0.58] and 0.38 [0.28-0.51], respectively; Ps<0.05). Conclusions: Our data indicate that patients with resectable BCLC B stage HCC and good liver function may benefit from surgical resection as another first-line option more curable than TACE.

사회공학적 이메일 공격 대비 모의훈련 시스템 설계

임일권 ( Il-kwon Lim ),김영혁 ( Young-hyuk Kim ),이재필 ( Jae-gwang Lee ),이재광 ( Jae-pil Lee ),남궁현 ( Hyun Nam-gung ),이재광 ( Jae-kwang Lee ) 한국정보처리학회 2014 한국정보처리학회 학술대회논문집 Vol.21 No.1

사회공학적 공격이란 인간의 심리를 이용하여 보안 위협 상황을 갖게 하는 공격을 말한다. 그렇기 때문에 사회공학적 공격을 막기 위한 보안 솔루션은 그 한계가 있기 마련이다. 그리하여 본 논문에서는 사회공학적 공격에 대비하는 보안훈련시스템을 제안한다. 스팸 및 피싱 이메일을 수집하여, 시그니처기반 필터링을 이용하여, 최신의 사회공학적 공격 이메일을 분석한 후, 가상으로 사회공학적 이메일 공격을 실시하여 훈련대상자들이 최신의 사회공학적 공격에 대비하는 능력을 갖추게 하는 보안 훈련 시스템을 설계하였다.

초음파 센서와 Kinect를 결합한 키 측정방식에 관한 연구

이재광(Jae-Gwang Lee),김영혁(Young-Hyuk Kim),임일권(Il-Kwon Lim),이재필(Jae-Pil Lee),이재광(Jae-Kwang Lee) 한국통신학회 2012 한국통신학회 학술대회논문집 Vol.2012 No.11