Xanthones from Garcinia paucinervis with in vitro anti-proliferative activity against HL-60 cells

Da-Hong Li,Chen-Xi Li,Cui-Cui Jia,Ya-Ting Sun,Chun-Mei Xue,Jiao Bai,Hui-Ming Hua,Xiao-Qiu Liu,Zhan-Lin Li 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.2

Three new xanthones, paucinervins H–J (1–3), as well as eleven known compounds (4–14), were isolated from the leaves of Garcinia paucinervis. The structures of the new compounds (1–3) were elucidated by 1D, 2D NMR spectra and HR ESIMS. In vitro antiproliferative activity against human promyelocytic leukemia HL-60 cells was tested, among which, compounds 2, 5, 6 and 7 exhibited strong growth inhibitory effects with GI50 values ranging from 1.30 to 9.08 lM, respectively. Preliminary SARs were also discussed.

Molecular Cloning and Expression of Grass Carp MyoD in Yeast Pichia pastoris

( Li Xin Wang ),( Jun Jie Bai ),( Jian Ren Luo ),( Hong Chen ),( Xing Ye ),( Qing Jian ),( Hai Hua Lao ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.1

The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2

Li, Yuxia,Bai, Hua,Zhang, Zhongzu,li, Weihua,Dong, Lei,Wei, Xueju,Ma, Yanni,Zhang, Junwu,Yu, Jia,Sun, Guotao,Wang, Fang Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.3

MicroRNAs (miRNAs) represent a class of small non-coding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

Effect of bamboo shoot dietary fiber on gel properties, microstructure and water distribution of pork meat batters

Li Ke,Liu Jun-Ya,Fu Lei,Zhao Ying-Ying,Zhu He,Zhang Yan-Yan,Zhang Hua,Bai Yan-Hong 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.7

Objective: To develop healthier comminuted meat products to meet consumer demand, the gel properties, rheological properties, microstructure and water distribution of pork meat batters formulated with various amounts of bamboo shoot dietary fiber (BSDF) were investigated. Methods: Different levels of BSDF (0% to 4%) were added to pork batters, and the pH, color, water-holding capacity, texture and rheological properties of pork batters were determined. Then, pork batters were analyzed for their microstructure and water distribution using scanning electron microscopy (SEM) and low-field nuclear magnetic resonance (LF-NMR). Results: Compared with the control, BSDF addition into meat batters showed a significant reduction in L*-value and a significant increase in b*-value (p<0.05). BSDF addition of up to 4% reduced the pH value of pork batters by approximately 0.15 units; however, the cooking loss and expressible water loss decreased significantly (p<0.05) with the increased addition of BSDF. The hardness and gel strength were noticeably enhanced (p<0.05) as the content of BSDF increased. The rheological results showed that BSDF added into pork batters produced higher storage modulus (G′) and loss modulus (G″) values. The SEM images suggested that the addition of BSDF could promote pork batters to form a more uniform and compact microstructure. The proportion of immobilized water increased significantly (p<0.05), while the population of free water was decreased (p<0.05), indicating that BSDF improved the water-holding capability of pork batters by decreasing the fraction of free water. Conclusion: BSDF could improve the gel properties, rheological properties and water distribution of pork meat batters and decrease the proportion of free water, suggesting that BSDF has great potential as an effective binder in comminuted meat products.

Compressive Deformation Behavior of AZ31Mg Alloy Containing {10–12} Extension Twins at Different Temperature

Hua Zhang,Xiaoqing Bai,Minjian Hou,Lifei Wang,Qiang Zhang,Jianfeng Fan,Weiguo Li,Hongbiao Dong,Bingshe Xu 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.5

Influence of pre-introducing {10–12} extension twins on compressive deformation behavior of AZ31Mg alloy at differenttemperature was investigated. The compression tests were conducted along the normal direction of AZ31Mg alloy at roomtemperature, 100 °C, 200 °C and 300 °C with a strain rate of 1 × 10−3 s−1. The results indicated that the pre-introducing{10–12} extension twins strongly affected the yield strength, the peak strength and the strain hardening rate at middle-lowtemperature (≤ 200 °C). The twinned samples containing pre-introducing {10–12} extension twins exhibited smaller yieldstrength and larger peak strength than the as-received samples without {10–12} extension twins at temperature ≤ 200 °C. Forthe as-received samples, the strain hardening rate decreased gradually at different temperature. While for the twinned samples,the strain hardening behavior exhibited three distinct stages at temperature ≤ 200 °C. When compressing at 300 °C, the asreceivedand twinned samples exhibited similar compression flow curves and strain hardening rate curves. The continuousdynamic recrystallization (CDRX) was the main dynamic recrystallization (DRX) mechanism in the as-received sample whencompressing at 200 °C. The twin assisted DRX besides CDRX was also initiated in the twinned sample when compressingat 200 °C. While the DRX mechanism was transformed into the discontinuous dynamic recrystallization (DDRX) in boththe as-received and twinned samples when compressing at 300 °C.

Separation of arenols from a low-temperature coal tar by liquid-liquid extraction

Hua-Shuai Gao,Zhi-Min Zong,Zheng Yang,Dao-Guang Teng,Xiu-Hua Sun,Li Yan,Xian-Yong Wei,Qing-Jie Guo,Tian-Sheng Zhao,Hong-Cun Bai 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5

Low-temperature coal tar (LTCT) is a sticky liquid mixture produced mainly from coal pyrolysis, which contains various value-added chemicals (VACs). Liquid-liquid extraction is considered as one of the green and effective ways to explore the organic composition and separate the VACs from LTCT. Herein, petroleum ether, methanol, and carbon disulfide were used to extract arenols from a LTCT. As a result, the relative content and absolute content of arenols extracted from the LTCT are ca. 96.3% and 85.9%, respectively. Among them, p-cresol is predominant, accounting for 22.2%. The isolated contents of arenols are up to 84.6%. Moreover, a kilogram-scale operation was carried out under the same conditions, which offers a potential application in industrial production.

Physiological responses of yellow‑horn seedlings to high temperatures under drought condition

Hua Jin,Jixiang Zou,Linlin Li,Xinlei Bai,Tong Zhu,Junbao Li,Bing-Cheng Xu,Zhi Wang 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Drought and hot stresses are the primary limiting factors for the growth and seed production of yellow-horn (Xanthoceras sorbifolia Bunge), especially in Xinjiang Province of China. A better understanding of its physiological and biochemical characteristics under drought–hot stress is requisite for its efficient cultivation in the arid and semi-arid areas. In this study, the physiological and biochemical responses of 3-month-old yellow-horn seedlings were evaluated after 7 days of treatments of five temperatures (25 °C/20 °C, 30 °C/25 °C, 35 °C/25 °C, 40 °C/25 °C and 45 °C/25 °C) under two watering conditions [adequate water supply (HW): 80 ± 5% FC (field capacity); low water supply (LW): 40 ± 5% FC] by a pot experiment. The increased temperature (above to 35 °C) markedly aggravated the damage on biomass accumulation and membrane integrity of yellow-horn seedlings under the LW. The accumulation of soluble sugars and proteins exhibited a growing trend as the temperature increased from 25 to 40 °C, which declined when the temperature was higher than 40 °C except for the soluble protein accumulation under HW. In addition, captured responsive characteristics of the gas-exchange parameters and chlorophyll fluorescence have indicated that the combinations of high temperature (above to 35 °C) and LW induced significant decrease in photosynthetic activities of yellow-horn seedlings. All these results showed that the hot stress significantly aggravated the drought damage on yellow-horn plant growth, especially when the temperature increased above 35 °C under drought stress.

(2019년 국제학술대회)桂林三戏”戏曲品牌文化旅游开发路径研究

Jin-hua Jiang(蒋,金桦,),Xiu-bai Li(李秀白) 융합관광콘텐츠학회 2019 융합관광콘텐츠학회 학술대회 발표집 Vol.2019 No.-

The purpose of the study to analyzes the problems existing in the development of opera cultural tourism resources on Three Operas in Guilin . First of all, the development model of opera culture resources is relatively simple and lacks brand image. Secondly, the interaction between the opera performance and the tourists is low, the performers rarely communicate with the tourists, the tourists have a weak understanding of the three local operas.Thirdly, the theater company has difficulties in operating and lacks professional drama talents. In addition, the older generation of artists is aging, which leads to the serious loss of traditional plays and weakens the attraction of local opera tourism development. Finally, the lack of propaganda and the lack of channels for the promotion of opera culture. Based on the above problems, this study explores the development path of the brand culture tourism of Three operas in Guilin . First, integrating opera cultural resources and regional tourism resources. Second, innovating drama culture tourism development model, using the art museum, theme park, opera experience hall and festival activities to create a Three operas in Guilin opera brand cultural tourism, enriching the Guilin local opera culture tourism model structure. Third, shaping the brand image of “Three operas in Guilin” and forming a cultural tourism project with Guilin characteristics. Fourth, improving the inheritance of the Three operas in Guilin and the system of training talents for opera, strengthening the interaction between opera performers and tourists, and increasing the attractiveness of tourists. Finally, broadening the publicity channels of traditional Chinese opera culture, strengthening the publicity, and improving tourists perception of traditional Chinese opera.

The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2

Yuxia Li,Hua Bai,Zhongzu Zhang,Weihua li,Lei Dong,Xueju Wei,Yanni Ma,Junwu Zhang,Jia Yu,Guotao Sun,Fang Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.3

MicroRNAs (miRNAs) represent a class of small non-co-ding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

Expression and Purification of Intact and Functional Soybean (Glycine max) Seed Ferritin Complex in Escherichia coli

( Xiang Bai Dong ),( Bo Tang ),( Jie Li ),( Qian Xu ),( Shen Tong Fang ),( Zi Chun Hua ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.2