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Differentially Expressed Genes under Cold Acclimation in Physcomitrella patens
Sun, Ming-Ming,Li, Lin-Hui,Xie, Hua,Ma, Rong-Cai,He, Yi-Kun Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6
Cold acclimation improves freezing tolerance in plants. In higher plants, many advances have been made toward identifying the signaling and regulatory pathways that direct the low-temperature stress response; however, similar insights have not yet been gained for simple nonvascular plants, such as bryophytes. To elucidate the pathways that regulate cold acclimation in bryophytes, we used two PCR-based differential screening techniques, cDNA amplified fragment length polymorphism (cDNA-AFLP) and suppression subtractive hybridization (SSH), to isolate 510 ESTs that are differentially expressed during cold acclimation in Physcomitrella patens. We used realtime RT-PCR to further analyze expression of 29 of these transcripts during cold acclimation. Our results show that cold acclimation in the bryophyte Physcomitrella patens is not only largely similar to higher plants but also displays distinct differences, suggests significant alteration during the evolution of land plants.
Hua Gu,Zhen-Hai Fei,Yi-Qi Wang,Jian-Guo Yang,Chao-Hui Zhao,Yong Cai,Xing-Ming Zhong 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.4
Purpose: Microvascular endothelial integrity is important for maintaining the blood-brain barrier (BBB). However, subarachnoid hemorrhage (SAH) disrupts this integrity, making the BBB dysfunctional—an important pathophysiological change after SAH. Angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) regulate microvascular permeability by balancing each other’s expression. Methods: This study investigated the dynamics of Ang-1 and Ang-2 expression after SAH and the protective effect of Ang-1 on BBB functioning using an endovascular puncture model of rat SAH. The Ang-1 and Ang-2 expression in brain tissue was determined by immunohistochemistry. In addition, Western blotting was used to estimate Ang-1 and Ang-2 concentration and to compare them at 6–72 hours post-SAH cortex and hippocampus. Evans blue viability assay was used to evaluate BBB permeability, and neurological testing was implemented to evaluate neurological impairment during SAH. Results: It was found that following SAH, Ang-1 expression decreases and Ang-2 expression increases in the cortex, hippocampus, and microvessels. The Ang-1/Ang-2 ratio decreased as quickly as 6 hours after SAH and reached its lowest 1 day after SAH. Finally, it was found that exogenous Ang-1 reduces SAH-associated BBB leakage and improves neurological function in post-SAH rats. Conclusions: Our findings suggest that the equilibrium between Ang-1 and Ang-2 is broken in a period shortly after SAH, and the treatment of exogenous Ang-1 injection alleviates neurological dysfunctions through decreasing BBB destruction.
Research on the Cutting Performances and Wear Mechanisms of TiAlCrN Coated Tools during Dry Turning
Hui-Bo He,Hua-Ying Li,Xian-Ying Zhang,Qi-Bin Yue,Jun Zhang,Lu Ma,Yuan-ming Li 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.2
Physical vapour deposition technique was used to deposit TiAlCrN coatings on the YT15 tungsten carbide inserts. The dry turning tests of 20CrMo steel were carried out to evaluate performances of TiAlCrN coated and uncoated tools on the CA6140A lathe. The effect of the two kinds of tools on cutting forces, cutting temperature, surface roughness and tool wear had been investigated to assess the performance of TiAlCrN coated tools. The results showed that the cutting force and cutting temperature obtained by TiAlCrN coated tools were decreased and the TiACrN coated tools produced a better surface finish in comparison with the uncoated tools. The TiAlCrN coated tools yield working life about 45 min, which was two times of that for uncoated tools. The wear mechanisms of the TiAlCrN coated tools were mainly oxidation and boundary wear, accompanied with diffusion wear.
A Study on Cutting and Tribology Performances of TiN and TiAlN Coated Tools
Hua-Ying Li,Hui-Bo He,Wen-Qiang Han,Jun Yang,Tao Gu,Yuan-ming Li,류성기 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.
TiN and TiAlN coatings were deposited on the surface of AISI 5140 steel and cemented carbide YT15 by magnetron sputteringtechnique (MSP). The reciprocating sliding tests of TiN and TiAIN coatings on the surface of AISI 5140 steel were performed toinvestigate the friction coefficients of coatings affected by various normal loads with friction pair of 304 stainless steel balls. Drymachining tests on AISI 5140 hardened steel were carried out with the TiN and TiAlN coated tools on a CA6140A lathe. The effectsof cutting speed on cutting forces and surface roughness of TiN and TiAlN coated tools were obtained and analyzed to assess thecutting performance of coated tools. The microcosmic micrographs of wear areas of coated tools were observed and investigated byscanning electron microscope and energy dispersive spectrum. The results show that the friction coefficients of TiN coatings are lowerthan that of TiAlN coatings. The cutting force of TiAlN coated tool decreases and flank wear resistance enhances in comparison withTiN coated tool. The wear form and mechanisms of TiN coated tool are mainly crater wear on the rake face and adhesive wear andabrasive wear on the flank face. The wear form and mechanisms of TiAlN coated tool are mainly adhesive wear, the breakage ofcutting edge and the damage of tip, accompanied with diffusion and oxidation wear.
( Hua Qi Pan ),( Su Ya Yu ),( Chun Feng Song ),( Nan Wang ),( Hui Ming Hua ),( Jiang Chun Hu ),( Shu Jin Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3
A new actinomycete strain NA4 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antifungal activities against soilborne fungal pathogens. It was identified as Streptomyces cavourensis by morphological, physiological, and phylogenetic analyses based on its 16S rRNA gene sequence. The main antifungal components were isolated and identified from the fermentation culture as bafilomycins B1 and C1. These compounds exhibited significant antifungal activities and a broad antifungal spectrum. The results suggest that the Streptomyces cavourensis NA4 and bafilomycins B1 and C1 could be used as potential biocontrol agents for soilborne fungal diseases of plants.
Shang-Hui Yang,Jian-Shian Lin,Fuh-Shyang Juang,Ding-Chin Chou,Ming-Hua Chung,Chen-Ming Chen,Lung-Chang Liu 한국물리학회 2013 Current Applied Physics Vol.13 No.5
In this paper, white light emitting diodes (LEDs) with good color rendering indices (CRI) and high luminous efficiencies have been fabricated by the encapsulation of mixed and double-deck phosphors. Experimental results revealed that white LEDs with the encapsulation of double-deck phosphors exhibited better CRI and higher luminous efficiencies than those with the encapsulation of mixed phosphors because no secondary excitation took place. The hue, CRI, and luminous efficiencies of white LEDs with double-deck phosphors under 200 mA were CIEx,y =(0.357, 0.348), 90, and 62.3 lm/W,respectively while the hue, CRI, and luminous efficiencies of white LEDs with mixed phosphors under 200 mA were CIEx,y = (0.366, 0.354), 89, and 56.5 lm/W, respectively.
Prognostic Significance of α5β1-integrin Expression in Cervical Cancer
Wang, Hua-Yi,Chen, Zhe,Wang, Zhu-Hui,Wang, Hong,Huang, Li-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
The purpose of this study was to evaluate the association of expression of ${\alpha}5{\beta}1$-integrin with clinicopathologic features and prognosis in cervical cancer. Levels of ${\alpha}5{\beta}1$-integrin in normal cervical mucosa and cervical cancer tissue were detected with immunohistochemistry. Survival analysis by the Kaplan-Meier method was performed to assess prognostic significance. ${\alpha}5{\beta}1$-integrin expression was detected in 84.6% (143/169) cervical cancer samples, significantly different from that in normal cervical mucosa (P < 0.05). Positive expression rates of ${\alpha}5{\beta}1$-integrin in patients with poor histologic differentiation, lymph node metastasis, and recurrence were elevated. Using Kaplan-Meier analysis, a comparison of survival curves of low versus high expression of ${\alpha}5{\beta}1$-integrin revealed a highly significant difference in human cervical cancer cases (P < 0.05), suggesting that overexpression of ${\alpha}5{\beta}1$-integrin is associated with a worse prognosis.The ${\alpha}5{\beta}1$-integrin promotes angiogenesis and associates with lymph node metastasis, vascular invasion and poor prognosis of cervical cancer. The current study indicated that ${\alpha}5{\beta}1$-integrin may be an independent prognostic factor for cervical cancer patients.
Dynamic Strain-Induced Ferrite Transformation during Hot Compression of Low Carbon Si-Mn Steels
Cai, Ming-Hui,Ding, Hua,Lee, Young-Kook The Japan Institute of Metals 2011 Materials transactions Vol.52 No.9
<P>The dynamic strain-induced transformation (DSIT) of austenite to ferrite was investigated under different undercooling conditions using three low carbon Si-Mn steels. The undercooling of austenite (Δ<I>T</I>) was controlled by varying the cooling rate between austenitization and deformation temperatures. Uniform DSIT ferrite grains (∼2.3 μm) were produced at a relatively high deformation temperature above 840°C using a low carbon high Si steel (0.077C-0.97Mn-1.35Si, mass%) in connection with a larger Δ<I>T</I>. The critical conditions for DSIT were determined based on the flow stress-strain curves measured during hot compression tests. Influence of deformation temperature on DSIT of low carbon Si-added steel was also discussed.</P>