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고영민,이내우,최재욱,우희철,신학기 한국산업안전학회 2002 한국안전학회지 Vol.17 No.1
Among the disposal techniques of inorganic wastewater sludges from industries, most of sludges were dealed with buring underground inefficiently. But we have tried to recycle the resources that exhausted inorganic sludges were properly blended to certain ratioes to make lightweight aggregate, that means manufacturing of it can be possible through blending of various components, drying, sintering, cooling and crushing to certain sizes that are needed by consumers. As disposal method of inorganic wastewater sludge is changed to environmentally fraternative method, this method could be very useful to make lightweight aggregates for decoration of building and the other so many purposes of those usages. That could be economically useful due to expected income for buring costs of ordinary disposal method from industries and sales income after manufacturing it. The second important profit is saving foreign currency from purchasing self-developed lightweight aggregate instead of importing expensive foreign product. This product will be also very impressive to user because of the fraternative lightweight to be composed of apatite structure that is important part of human body.
入力電流의 高調波 低減을 위한 새로운 다이리스터 整流回路의 개발
柳喆魯,金聖中,李公熙,李星龍,高榮浩 全北大學校 1987 論文集 Vol.29 No.-
The harmonics involved in input AC line currents of thyristor rectifier apparatus have recently been a serious problem in the cause of burning power condenser, mis-action of UFR(protective relay), and trouble of the automatic voltage controller, etc, in the sending or distribution electric power system. As an effective counter-measure for this, the method of having switching taps on the conventional interphase reactor and its artificial utilization have been adapted, as we know. The conventional 3 tap method, however, is not achieved an effect of reducing harmonics under firing angle 15˚. In this paper, a new method of reducing harmonics involved in input AC line currents or output DC voltage of rectifier circuit is presented, which is due mainly to the equipment of the 2-3 switching taps on interphase reactor. Thereofre, main contents of this study are the following three items; 1) optimal number of taps 2) optimal turn ratio of interphase reactor 3) optimal control angle of interphase reactor.
Effect of human endogenous retrovirus-K env gene knockout on proliferation of ovarian cancer cells
Ko Eun-Ji,Kim Eun Taeg,Kim Heungyeol,Lee Chul Min,Koh Suk Bong,Eo Wan Kyu,Kim Hongbae,Oh Young Lim,Ock Mee Sun,Kim Ki Hyung,Cha Hee-Jae 한국유전학회 2022 Genes & Genomics Vol.44 No.9
Background: Among various human endogenous retroviruses (HERVs), the HERV-K (HML-2) group has been reported to be highly related to cancer. In pancreatic cancer cells, shRNA-mediated downregulation of HERV-K env RNA decreases cell proliferation and tumor growth through the RAS-ERK-RSK pathway; in colorectal cancer, CRISPR-Cas9 knockout (KO) of the HERV-K env gene affects tumorigenic characteristics through the nupr-1 gene. Objective: The effect of HERV-K env KO has not been studied in ovarian cancer cell lines. In this study, we analyzed the tumorigenic characteristics of ovarian cancer cell lines, including cell proliferation, migration, and invasion, and the expression patterns of related proteins after CRISPR-Cas9 KO of the HERV-K env gene. Methods: The HERV-K env gene KO was achieved using the CRISPR-Cas9 system in ovarian cancer cell lines SKOV3 and OVCAR3. Tumorigenic characteristics including cell proliferation, migration, and invasion were analyzed, and related protein expression was investigated by western blot analysis. Results: The expression of the HERV-K env gene in KO cells was significantly reduced at RNA and protein levels, and tumorigenic characteristics including cell proliferation, migration, and invasion were significantly reduced. In HERV-K env KO SKOV3 cells, the expression of the RB protein was significantly up-regulated and the cyclin B1 protein level was significantly reduced. In contrast, in HERV-K env KO OVCAR3 cells, the level of phospho-RB protein was significantly reduced, but other protein levels were not changed. Conclusion: The results of this study showed that HERV-K env gene KO affects cell proliferation, invasion, and migration of ovarian cells through RB and Cyclin B1 proteins, but the specific regulation pattern can differ by cell line.
Jung-Hee Ko,Hee-Young Jang,Kun Cho,Seong-Woo Cho,Jong-Soon Choi,Toshiaki Mitsui,Chul-Won Lee,Yong-Gu Cho,Sun-Hee Woo 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
High temperature impediment in developing stages of crops has been occurred due to the impact of global warming. Rice production is notable to be sensitive to increasing environmental temperature and grain filling temperatures are already approaching threatening levels in many countries with rice cultivation. Recent proteomic analyses exposed impulsive changes of metabolisms during rice grain development. Interestingly, proteins involved in glycolysis, citric acid cycle, lipid metabolism, and proteolysis were accumulated at higher levels in mature grain than those of developing stages. High temperature (HT) stress in rice ripening period enhances damaged (chalky) grains which have loosely compacted shape starch granules. We carried out two-dimensional gel electrophoresis to analyze protein profiles during grain filling and different developmental stages of rice seed maturation. Proteins were separated from the fertilized seeds (seeds from 7 days and 21 days after fertilization) and seed maturation stage using IEF in the first-dimension and SDS-PAGE in the second dimension along with MALDI-TOF mass spectrometry. More than 1,000 protein spots were detected on a two-dimensional gel electrophoresis. A total of 120 different protein spots out of 140 protein spots were identified by MALDI-TOF and nano LCQ-TOF mass spectrometer. The identified proteins were categorized into six (6) different groups according to their expression patterns during grain filling and seed maturation. Some proteins were confirmed during seed development stages such as cytoplasmic malate dehydrogenase, whereas others were appeared at a specific stage like putative subtilisin-like protease, germin-like, seed allergenic proteins. Furthermore, the chalking mechanism of rice grain under the HT stress could be discussed in terms of grain starch glycome, transcriptome, and proteome.
고희철(Hee-Chul Ko) 동아시아식생활학회 2010 동아시아식생활학회지 Vol.20 No.6
This study examined the effects of Cornus fructus addition to suger snap cookies on WRC (water rentention capacity) and AWRC (alkaline water retention capacity). WRC and AWRC increased as more Cornus fructus powder was added, whereas the sedimentation value and Pelshenke value decreased. Regarding cookie color, L value decreased, whereas the a and b values increased. As the cookie diameter became smaller, thickness increased and spread factor decreased. Hardness of the cookies decreased, and preference was highest for cookies with 5% added Cornus fructus. The preferences for cookies were in the following order: 7%<3%<1%<0%<5% addition. In conclusion, addition of less than 5% Cornus fructus powder was the most desirable. To complement cookie appearance, it seems necessary to adjust the water content of Cornus fructus powder or add an emulsifier.