수종의 피부종양에서 Proliferating Cell Nuclear Antigen과 Ki - 67 의 표현에 관한 비교 연구

황선욱(Sun Wook Hwang),원영호(Young Ho Won),전인기(Inn Ki Chun),박창수(Chang Soo Park) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.3

Background : Both PCNA and Ki-67 have been used as marker for cellular proliferation. The drawback of Ki-67 antibody in immunohistochemistry was that it can be labelled only on fresh tissue, however, MIB1 is a newly developed Ki-67 antiboc which can be labelled on formalin-fixed tissue. Objective : The purpase of the present study is to compir the stainability of the Ki-67 antibody with that of the ICNA antibody on formalin-fixed, para fin embedded tissues. Methods : Using MIE1, the newly developed Ki-67 antibody and PC10(PCNA antibody), speci mens of squamous cell carcinoma(SCC), Bowens disease(BL), actinic keratosis(AK) and basal cell epithelioma(BCE) were stained by one hour immunocytial, mistry using a Microprobe immuno/DNA stainer. Results : The labelling indices (LI) of MIB1 were 82.6%, 37.4%, 38.3% & 81.1% respectively in SCC, BD, AK & BC, while the LI of PC10 were 77.69%, 26.6% & 64.4%. The labelled cells of both antibodies differed in distribution patterns on turmor tissues. Conclusion : MIBI cain be used to be an alternative m.rl r for proliferating cells. MIBI PC10, when used together, will be mutually compensatory the study of proliferating cell kinetics. (Kor J Dermatol 1995;33(3): 453-458)

Ki-67 Expression Gives Additional Prognostic Information on St. Gallen 2007 and Adjuvant! Online Risk Categories in Early Breast Cancer

Jung, So-Youn,Han, Wonshik,Lee, Jong Won,Ko, Eunyoung,Kim, Eunkyu,Yu, Jong-Han,Moon, Hyeong-Gon,Park, In Ae,Oh, Do-Youn,Im, Seock-Ah,Kim, Tae-You,Hwang, Ki-Tae,Kim, Sung-Won,Noh, Dong-Young Springer - Society of Surgical Oncology 2009 Annals of Surgical Oncology Vol.16 No.5

필라테스 운동이 여대생의 신체구성과 하지근력 및 척추 형태에 미치는 영향

황윤영(Hwang, Yoon-Young),박기덕(Pack, Gi-Duck),임기원(Lim, Ki-Won) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.5

This study is to provide the preliminary data verifying Pilates effect analyzing the effect of the warm-up, exercise and warm-down were conducted for 50 minutes a day, three time a week and for ten week. Pilates exercises for female university students on their body compositions, changes in the spine and muscular strength. The study is to involve female university students, majoring in general department (non-Physical Education) who take liberal arts classes in K and N university, and it was distinguished between 9(PG: age(20.6±0.9), height (160.9±7.1), weight(53.9±4.5)) female university students who will participate in Pilates Exercise Program and 9(CG: age(22.2±1.4), height(159.9±5.4), weight(52.3±4.7)) female university students who won"t. First, There were insignificant differences in body composition factor. Second, There were significant differences in Peak torque from isokinetic muscular function factor, Total work and Average power. Third, There were insignificant differences in 3-dimension spine body type factor. Through the ten-week Pilates exercises for three times a week, there weren"t significant differences in the body composition and positive effect of isokinetic muscular function factor, and the 3-dimension spine body type was shown.

중,홍콩 무역업자의 무역결제시 위안화 선호도에 관한 연구

구기보 ( Ki Bo Ku ),황원일 ( Won Il Hwang ) 한국외국어대학교 국제지역연구센터 중국연구소 2010 中國硏究 Vol.49 No.-

In this study we drew conclusions for the international trade settlement between Korea and China by investigating the preference of Hong-Kong residents for Chinese Yuan as the key currency in the trade between China and Hong-Kong. The questionnaire was designed to ask about convenience, value stability, and liquidity of Yuan, and businessmen who were engaged in foreign trade at Hong-Kong participated in this survey. Since the subprime crisis began in the US, the Chinese government has driven the policy for the internationalization of Yuan, including the international trade settlement through Yuan. Even though the international trade settlement through Yuan was not successful, as of November 2009, because of its technical problems, insufficient follow-up actions, ill-refined foreign exchange control procedures, general difficulty in money exchange, and preference of importers for US dollars, the empirical study showed that Yuan was highly preferred as the key currency in the trade between China and Hong-Kong. The conclusions drawn from the role of Yuan as the key currency in the trade between China and Hong-Kong are as follows: First, using Yuan and/or Won as the key currency in the trade between Korea and China would prevent trade-shrinking due to the shortage of US dollars. Second, businessmen who are engaged in the trade between Korea and China would expect the convenience of currency increased by using Yuan and/or Won. Third, the risk of foreign exchange loss would be decreased by using Yuan and/or Won because the currency value of Yuan would be relatively stable compared to US dollars. Fourth, the role of Yuan as the key currency in foreign trade would be increased in the near future, and the necessity of relying on US dollars would be relatively decreased. Finally, the systems and procedures that are related to using Yuan and/or Won as the key currency in the trade between Korea and China should be well established.

Effects of High Cholesterol Diet on Newly Generated Cells in the Dentate Gyrus of C57BL/6N and C3H/HeN Mice

KIM, Il Yong,HWANG, In Koo,CHOI, Ji Won,YOO, Ki-Yeon,KIM, Yo Na,YI, Sun Shin,WON, Moo-Ho,LEE, In Se,YOON, Yeo Sung,SEONG, Je Kyung Japanese Society of Veterinary Science 2009 The Journal of veterinary medical science Vol.71 No.6

<P>In this study, we observed and compared the effects of a high cholesterol diet (HCD) on cell proliferation and differentiation in the subgranular zone of the dentate gyrus of C57BL/6N (B6, susceptible strain) and C3H/HeN (C3H, resistant strain) mice. Ki67 (a marker for cell proliferation) positive cells) were significantly decreased in HCD-fed B6 mice compared to those in B6 (49.7%) and C3H mice fed a low cholesterol diet (LCD). In addition, doublecortin (DCX, a marker for cell differentiation or neuroblasts)-immunoreactive cells in HCD-fed B6 mice were significantly decreased compared to those in LCD-fed B6 and C3H mice. These results suggest that B6 strains are sensitive to HCD, which impairs cell proliferation and differentiation.</P>

Long-term postresection prognosis of primary neuroendocrine tumors of the liver

Jay Jung,Shin Hwang,Seung-Mo Hong,Ki-Hun Kim,Chul-Soo Ahn,Deok-Bog Moon,Tae-Yong Ha,Gi-Won Song,Yo-Han Park 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.97 No.4

Purpose: Primary hepatic neuroendocrine tumor (PHNET) is a very rare neoplasm, requiring strict exclusion of metastasis from possible extrahepatic primary sites for its diagnosis. Methods: We reviewed our clinical experience of 13 patients with primary hepatic NET who underwent liver resection from January 1997 to December 2015. Results: The mean age of the 13 patients (8 males and 5 females) was 51.1 ± 12.8 years; the most common clinical manifestation was vague, nonspecific abdominal pain (n = 9). Of them, 11 patients underwent preoperative liver biopsy, 7 of which correctly diagnosed as neuroendocrine tumor (NET). Ten patients underwent R0 resection, and 3 underwent R1 resection. Diagnosis of PHNET was confirmed both immunohistochemically and by absence of extrahepatic primary sites. All tumors were single lesions, with a mean size of 9.6 ± 7.6 cm and a median size of 4.3 cm; all showed positive staining for synaptophysin and chromogranin. During a mean follow-up period of 95.1 ± 86.6 months, 7 patients died from tumor recurrence, whereas the other 6 remain alive to date, making the 5-year tumor recurrence rate 56.0% and the 5-year patient survival rate 61.5%. When confined to R0 resection, 5-year recurrence and survival rates were 42.9% and 70.0%, respectively. Univariate analysis showed that Ki-67 proliferative index was the only risk factor for tumor recurrence. Conclusion: PHNET is a very rare tumor with no specific clinical features, and its final diagnosis depends primarily on pathology, immunohistochemistry, and exclusion of metastasis from other sites. Aggressive surgical treatment is highly recommended for PHNET because of acceptably favorable postresection outcomes

Corrigendum to “Vertebral Body Anterior Translation, a Novel Technique for Delayed Myelopathy Due to Osteoporotic Spine Fractures”

Jung Soo Lee,Dong Ki Ahn,Won Shick Shin,Kyung Jun Cho,Young Rok Ko,Il Chan Hwang 대한정형외과학회 2022 Clinics in Orthopedic Surgery Vol.14 No.4

Clinics in Orthopedic Surgery 2020;12:485-492 https://doi.org/10.4055/cios20028 In the article entitled “Vertebral body anterior translation, a novel technique for delayed myelopathy due to osteoporotic spine fractures,”1) the name of one of the authors was incorrectly presented: Il Chan Whang should read Il Chan Hwang.

Effects of Streptozotocin-Induced Type 1 Diabetes on Cell Proliferation and Neuronal Differentiation in the Dentate Gyrus : Correlation with Memory Impairment

Jung Hoon Choi,In Koo Hwang,Sun Shin Yi,Ki-Yeon Yoo,Choong Hyun Lee,Hyung-Cheul Shin,Yeo Sung Yoon,Moo-Ho Won 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.1

We examined the effects of steptozotocin (STZ)-induced type 1 diabetes on cell proliferation and neuroblasts in the subgranular zone of the hippocampal dentate gyrus (SZDG) of male Wistar rats. Change in memory function was also investigated using the passive avoidance test. In the SZDG, Ki67 (a marker for cell proliferation) positive nuclei were significantly decreased at 2 and 3 weeks and slightly decreased at 4 weeks after STZ treatment. Doublecortin (DCX, a marker for neuronal differentiation)-immunoreactive (+) neuroblasts with tertiary dendrites were significantly decreased in the STZ-treated group compared to those in the vehicle-treated group. However, DCX+ neuroblasts without tertiary dendrites were abundant at 4 weeks after STZ treatment. In addition, retention latency time in STZ-treated group was similar to that of vehicle-treated group at 2 and 3 weeks after STZ treatment. However, the retention latency time was significantly decreased at 4 weeks after STZ treatment. These results suggest that STZ significantly reduced cell proliferation and neuroblasts at 2~3 weeks after STZ treatment, but not at 4 weeks after STZ treatment although memory impairment was detected at 4 weeks after STZ treatment. The gradual reduction of DCX+ neuroblasts with tertiary dendrites may be associated with the impairment of hippocampus-related memory function.

Effects of pyridoxine on a high‐fat diet‐induced reduction of cell proliferation and neuroblast differentiation depend on cyclic adenosine monophosphate response element binding protein in the mouse dentate gyrus

Yoo, Dae Young,Kim, Woosuk,Yoo, Ki‐,Yeon,Nam, Sung Min,Chung, Jin Young,Yoon, Yeo Sung,Won, Moo‐,Ho,Hwang, In Koo Wiley Subscription Services, Inc., A Wiley Company 2012 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.90 No.8

<P><B>Abstract</B></P><P>In this study, we challenged pyridoxine to mice fed a high‐fat diet (HFD) and investigated the effects of pyridoxine on HFD‐induced phenotypes such as blood glucose, reduction of cell proliferation and neuroblast differentiation in the dentate gyrus using Ki67 and doublecortin (DCX), respectively. Mice were fed a commercially available low‐fat diet (LFD) as control diet or HFD (60% fat) for 8 weeks. After 5 weeks of LFD or HFD treatment, 350 mg/kg pyridoxine was administered for 3 weeks. The administration of pyridoxine significantly decreased body weight in the HFD‐treated group. In addition, there were no significant differences in hepatic histology and pancreatic insulin‐immunoreactive (‐ir) and glucagon‐ir cells of the HFD‐treated group after pyridoxine treatment. In the HFD‐fed group, Ki67‐positive nuclei and DCX‐ir neuroblasts were significantly decreased in the dentate gyrus compared with those in the LFD‐fed mice. However, the administration of pyridoxine significantly increased Ki67‐positive nuclei and DCX‐ir neuroblasts in the dentate gyrus in both LFD‐ and HFD‐fed mice. In addition, the administration of pyridoxine significantly increased the protein levels of glutamic acid decarboxylase 67 (GAD67) and brain‐derived neurotrophic factor (BDNF) and the immunoreactivity of phosphorylated cyclic AMP response element binding protein (pCREB) compared with the vehicle‐treated LFD‐ and HFD‐fed mice. In contrast, the administration of pyridoxine significantly decreased HFD‐induced malondialdehyde (MDA) levels in the hippocampus. These results showed that pyridoxine supplement reduced the HFD‐induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus via controlling the levels of GAD67, pCREB, BDNF, and MDA. © 2012 Wiley Periodicals, Inc.</P>

Substance P restores normal skin architecture and reduces epidermal infiltration of sensory nerve fiber in TNCB-induced atopic dermatitis-like lesions in NC/Nga mice

Choi, Hyeongwon,Kim, Dong-jin,Nam, Seungwoo,Lim, Sunki,Hwang, Jae-Sung,Park, Ki Sook,Hong, Hyun Sook,Won, Younsun,Shin, Min Kyung,Chung, Eunkyung,Son, Youngsook Elsevier 2018 JOURNAL OF DERMATOLOGICAL SCIENCE Vol.89 No.3

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by intense pruritus and eczematous lesion. Substance P (SP) is an 11-amino-acid endogenous neuropeptide that belongs to the tachykinin family and several reports recently have supported the anti-inflammatory and tissue repairing roles of SP.</P> <P><B>Objective</B></P> <P>In this study, we investigated whether SP can improve AD symptoms, especially the impaired skin barrier function, in 2, 4, 6-trinitrochlorobenzene (TNCB)-induced chronic dermatitis of NC/Nga mice or not.</P> <P><B>Method</B></P> <P>AD-like dermatitis was induced in NC/Nga mice by repeated sensitization with TNCB for 5 weeks. The experimental group designations and topical treatments were as follows: vehicle group (AD-VE); SP group (AD-SP); and SP with NK1R antagonist CP99994 (AD-SP-A) group. Histological analysis was performed to evaluate epidermal differentiation, dermal integrity, and epidermal nerve innervation in AD-like lesions. The skin barrier functions and pruritus of NC/Nga mice were evaluated by measuring transepidermal water loss (TEWL) and scratching behavior, respectively.</P> <P><B>Result</B></P> <P>Topical SP treatment resulted in significant down-regulation of Ki67 and the abnormal-type keratins (K) K6, K16, and K17, restoration of filaggrin and claudin-1, marked reduction of TEWL, and restoration of basement membrane and dermal collagen deposition, even under continuous sensitization of low dose TNCB. In addition, SP significantly reduced innervation of itch-evoking nerve fibers, gelatinase activity and nerve growth factor (NGF) expression in the epidermis but upregulated semaphorin-3A (Sema3A) expression in the epidermis, along with reduced scratching behavior in TNCB-treated NC/Nga mice. All of these effects were completely reversed by co-treatment with the NK1R antagonist CP99994. In cultured human keratinocytes, SP treatment reduced expression of TGF-α, but upregulated TGF-β and Sema3A.</P> <P><B>Conclusion</B></P> <P>Topically administered SP can restore normal skin barrier function, reduce epidermal infiltration of itch-evoking nerve fibers in the AD-like skin lesions, and alleviate scratching behavior. Thus, SP may be proposed as a potential medication for chronic dermatitis and AD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Topical Substance P treatment reduced AD-like symptoms in TNCB-induced NC/Nga mice. </LI> <LI> Topical Substance P treatment reduced epidermal acanthosis and expressed normal keratin profiles in AD-like mice. </LI> <LI> Topical Substance P treatment regulated both small nerve attraction and repulsion factors in AD-like skin. </LI> <LI> Topical Substance P treatment recovered skin barrier function and reduced pruritus in AD-like mice. </LI> </UL> </P>