Expression of three neuropeptide genes (SfEH, SfETH, and SfCCAP) in response to insecticide stress on the white-backed planthopper Sogatella furcifera (Hemiptera: Delphacidae)

Long Gui-yun,Wang Zhao,Yang Jia-peng,Tang Chen-hong,Jin Dao-Chao,Yang Hong,Zhou Cao,Yang Xi-bin 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.3

Insect neuropeptides are involved in many important developmental processes. Here, we cloned three neuro peptide genes (SfEH, SfETH, and SfCCAP) from Sogatella furcifera (Horváth) and analyzed the relative expression levels of these genes through Real-time Quantitative polymerase chain reaction (RT-qPCR) during different developmental stages and in different wing types of S. furcifera following exposure to sublethal concentrations (LC 10 and LC 25 ) of methylaminoavermectin benzoate. The results revealed that the three neuropeptide genes (SfEH, SfETH, and SfCCAP) of S. furcifera contained open reading frame sequences of 222, 636, and 408 bp, encoding 73, 211, and 135 amino acids, respectively. Moreover, the expression of SfEH and SfETH reached its peak on day 3 of the 5 th instar nymph stage, while the SfCCAP expressed pinnacle on 40 min after eclosion. Furthermore, the relative expression levels of the three genes were in the following order: SfEH, short-winged female > long-winged female > long-winged male; SfETH, long-winged female > short-winged female > longwinged male; and SfCCAP, short-winged female > long-winged male > short-winged female. Upon calculating the relative expression of the three peptide genes after 24 and 48 h of treatment with sublethal concentrations of methylaminoavermectin benzoate, it was found that the relative mRNA expression levels of SfEH and SfETH declined upon LC 10 treatment but increased upon LC 25 treatment. Meanwhile, the relative mRNA expression level of SfCCAP declined upon both LC 10 and LC 25 treatments. This result will help us understand the effects of pesticides on insect growth and development at the molecular level.

스마트 수면 보조제품의 서비스 시스템 비즈니스 모델 연구

용가흥 ( Long¸ Jia Xing ),이성필 ( Lee¸ Sung Pil ) 한국기초조형학회 2021 기초조형학연구 Vol.22 No.5

서울아산병원 조사에 의하면 수면장애 된 사람이 크게 늘었으며, 특히 젊은 직장인이 직업과 가족의 빠른 발전에 중요한 단계에 있어 수면장애의 비율이 크게 늘고 있는 실정이다. 장기적인 수명 장애는 사람들의 일상 활동과 신체 건강에 심각한 영향을 미칠 것이다. 그러므로, 수면에 주의를 기울이고 수면과 건강의 문제를 연구하는 것이 필요할 것이다. 이러한 이유로 다양한 수면 보조제품과 맞춤형 수면 콘텐츠에 대한 수요도가 증가하고 있고 스마트 제품의 빠른 발전은 수면 제품의 디자인과 개선에 새로운 전환점이 되었다. 본 연구의 목적은 지속가능한 제품 서비스 시스템(PSS)의 관점에서 새로운 수면장애 제품에 대한 서비스모델을 개발하는 것으로서 구체적인 연구방법 및 진행내용으로 첫째, 이해관계자 간의 상호작용 관계에 대하여 분석하였다. 왜 수면에 문제가 있는지에 대한 주제를 중심으로 자유롭게 토론하는 방법으로 아이디어와 키워드를 도출하였고 기존 수면 제품에 대한 조사 및 분석을 통하여 맞춤형 수면 제품의 장점과 단점에 대하여 분석하였다. 둘째, 대상자에 대한 분석을 통하여 페르소나에 대한 시나리오를 구체화하였고 고객 심층 인터뷰와 조사를 통하여 8개 분야의 키워드 중 총 30개의 문제점과 요구사항을 파악하였다. 마지막으로 KJ 방법을 활용하여 복합적인 통찰력을 유기적으로 결합 및 귀납하고 사용자 만족도 조사를 통하여 핵심 가치를 도출 및 다자간 협업과 상생으로 수면의 질을 효과적으로 개선할 수 있는 ‘스마트 수면 보조제품 서비스 시스템 비즈니스 모델’을 제안하였는데 도출한 주요 내용으로는 수면 환경, 질 좋은 수면을 위한 방법에 대한 지적 부재 등 7가지 이슈가 주요 요인이었다. 최종적으로 7가지 문제점을 해결하기 위한 스마트 수면 기술의 활용 내용과 과정을 시각화하였다. According to a survey at Asan Medical Center in Seoul, the number of people with sleep disorders has increased significantly, and in particular, the rate of sleep disorders is increasing significantly among young office workers as they are at an important stage in the rapid development of their jobs and families. Long-term lifespan impairments will seriously affect people's daily activities and physical health. Therefore, it will be necessary to pay attention to sleep and study the problems of sleep and health. For this reason, the demand for various sleep aids and customized sleep contents is increasing, and the rapid development of smart products has become a new turning point in the design and improvement of sleep products. The purpose of this study is to develop a service model for a new sleep disorder product from the perspective of sustainable Product Service System(PSS). First, the interaction relationship between stakeholders was analyzed with specific research methods and progress. Ideas and keywords were derived through a free discussion method focusing on the topic of why there is a problem with sleep, and the advantages and disadvantages of customized sleep products were analyzed through investigation and analysis of existing sleep products. Second, the scenario for persona was specified through the analysis of the subjects, and a total of 30 problems and requirements among the keywords in 8 fields were identified through in-depth customer interviews and surveys. Third, an intelligent sleep aid product service system business model that organically combines and inducts complex insights using the KJ method, derives core values through user satisfaction surveys, and effectively improves sleep quality through multilateral collaboration and coexistence was proposed, but as the main content derived, seven issues were the main factors, including the sleep environment and the lack of intellectual knowledge on methods for good quality sleep. Finally, the contents and process of using smart sleep technology to solve seven problems were visualized.

Roles of NMDA NR2B Subtype Receptor in Prefrontal Long-Term Potentiation and Contextual Fear Memory

Zhao, Ming-Gao,Toyoda, Hiroki,Lee, Yong-Seok,Wu, Long-Jun,Ko, Shanelle W.,Zhang, Xue-Han,Jia, Yongheng,Shum, Fanny,Xu, Hui,Li, Bao-Ming,Kaang, Bong-Kiun,Zhuo, Min Elsevier 2005 Neuron Vol.47 No.6

<P><B>Summary</B></P><P>Cortical plasticity is thought to be important for the establishment, consolidation, and retrieval of permanent memory. Hippocampal long-term potentiation (LTP), a cellular mechanism of learning and memory, requires the activation of glutamate N-methyl-D-aspartate (NMDA) receptors. In particular, it has been suggested that NR2A-containing NMDA receptors are involved in LTP induction, whereas NR2B-containing receptors are involved in LTD induction in the hippocampus. However, LTP in the prefrontal cortex is less well characterized than in the hippocampus. Here we report that the activation of the NR2B and NR2A subunits of the NMDA receptor is critical for the induction of cingulate LTP, regardless of the induction protocol. Furthermore, pharmacological or genetic blockade of the NR2B subunit in the cingulate cortex impaired the formation of early contextual fear memory. Our results demonstrate that the NR2B subunit of the NMDA receptor in the prefrontal cortex is critically involved in both LTP and contextual memory.</P>

A Novel Self-Healing Polyurethane with High Transparency and Strength: Effects of Multiple Supermolecular Forces

Yuan Liu,Yanmei Liao,Yintao Long,Yuxiang Qian,Shengqiang Nie,Yi Wang,Jia Zeng,Chunmei Zhang,Linbo Tian,Jun Luo 한국고분자학회 2022 폴리머 Vol.46 No.3

It is a considerable challenge for expanding the applications of self-healing polyurethane because of the dilemma in balancing its mechanical and self-healing properties. Herein, a series of self-healing polyurethane systems (PUT-x) with short-range ordered rigid aromatic structures were synthesized by simply adjusting two kinds of diisocyanates, and by ingenious addition of aromatic chain extender. We hope to regulate the π-π interactions and further to manipulate the properties of obtained polyurethanes via adjusting the amount of the benzene rings in the molecular chains of the PUT-x systems. The PUT-5 with decent self-healing ability, optimal mechanical properties, acceptable thermal stability and favorable transparency was selected to incorporate liquid metal and fluorescent powder to fabricate conductive wire and anti-counterfeiting film. Significantly, the liquid metal could be recycled from the conductive wire conveniently without using strong acids or strong bases. Moreover, PUT-5 exemplarily withstood long-term soaking in normal saline, and no deformation could be identified.

Changes in Markers of Liver Function in HCV 1b Patients with Compensated Cirrhosis Treated with Ombitasvir/Paritaprevir/ Ritonavir plus Dasabuvir with Ribavirin

( Jeong Heo ),( Yan Luo ),( Wan-long Chuang ),( Jidong Jia ),( Kwang-hyub Han ),( Ming-lung Yu ),( Hong Tang ),( Young-suk Lim ),( Cheng-yuan Peng ),( Min Xu ),( Maorong Wang ),( Bo Fu ),( Niloufar Mo 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Patients chronically infected with HCV are at risk of developing extrahepatic manifestations of HCV as well as progressing to compensated or decompensated cirrhosis and HCC. Although current treatments have high rates of SVR, relatively little is known about possible regression of liver fibrosis after achieving an SVR. The ONYX-II trial examined the efficacy and safety of ombitasvir/paritaprevir/ritonavir plus dasabuvir + ribavirin (RBV) in Asian patients with HCV genotype 1b infection and compensated cirrhosis. Here we report changes in key markers of liver fibrosis and function. Methods: Patients with chronic HCV GT1b infection and compensated cirrhosis were enrolled in China, South Korea and Taiwan and received 12 weeks of OBV/PTV/r (25 mg/150 mg/100 mg once daily) and DSV (250 mg twice daily) with weight-based RBV. The primary objective of ONYX-II was to assess efficacy (SVR12) and safety of the regimen. Changes in markers of liver fibrosis and function between baseline (BL) and post-treatment week (PTW) 12 are presented. Results: Overall, 104 patients were enrolled and treated in ONYX-II. All patients (104/104, 100%) achieved SVR12. BL and PTW12 data for FibroTest score, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, albumin, platelet count and alpha fetoprotein (AFP) are shown in Table 1. All selected parameters showed numerical improvements between BL and PTW12. Mean ALT and AST levels returned to within normal range and FibroTest scores demonstrated a numerical improvement, suggesting improvement in liver status. The complete set of data between BL and PTW12 will be presented for these parameters and other liver composite parameters at the conference. Conclusions: Measurement of key liver function markers during the ONYX-II trial showed a numerical improvement within 12 weeks of completion of treatment in HCV GT1b-infected patients with compensated cirrhosis. Further follow-up of these patients will determine the long-term durability of these changes.

Case Report : Nutrition Therapy for Mitochondrial Neurogastrointestinal Encephalopathy with Homozygous Mutation of the TYMP Gene

( Jing Wang ),( Wei Chen ),( Fang Wang ),( Dong Wu ),( Jia Ming Qian ),( Jun Ren Kang ),( Hai Long Li ),( En Ling Ma ) 한국임상영양학회 2015 Clinical Nutrition Research Vol.4 No.2

Mitochondrial neurogastrointestinal encephalopathy (MNGIE) is characterized by significant gastrointestinal dysmotility. Early and long-term nutritional therapy is highly recommended. We report a case of MNGIE in a patient who was undergoing longterm nutrition therapy. The patient was diagnosed with a serious symptom of fatty liver and hyperlipidemia complications, along with homozygous mutation of the thymidine phosphorylase (TYMP) gene (c.217G > A). To our knowledge, this is the first report of such a case. Herein, we describe preventive measures for the aforementioned complications and mitochondrial disease-specific nutritional therapy.

Analysis of Magnetic Particle Agglomeration Structure and Interaction Forces Between Magnetic Particles

Jia Long,Zixu Miao,Huihuang Chen,Rongdong Deng,Weiran Zuo,Bao Guo,Jiangang Ku 한국자기학회 2020 Journal of Magnetics Vol.25 No.1

Chain-like and diamond-shaped magnetic particle agglomeration (MPA) commonly forming in a weak magnetic field are simulated based on the finite element method (FEM), and the effects of particle diameter, magnetic field strength, particle relative magnetic permeability, and particle number in magnetic particle chains (MPCs) and diamond-shaped MPA on the strength of MPA are analysed in detail. The results show that magnetic forces on the centre contact points (CCPs) of MPA are positively correlated with the particle diameter, magnetic field strength, particle relative magnetic permeability, and particle number. In addition, the forces on the CCPs of the MPCs (N=2) have a square relationship with the particle diameter and magnetic field strength and have a power relationship of 1.25 with the particle relative magnetic permeability. The forces on each contact point decrease slowly from the centre to both ends in the MPCs and then rapidly decrease to one value (approximately 0.779 times the forces on the CCPs). As for the diamond-shaped MPA, with the increase in the angle α between the magnetic field and axis of diamond-shaped MPA, the force magnitude of the particle entrained parallelly in the diamond-shaped MPA shows a trend of a “cosine curve” shape and the minimum value is 2109 times that of the entrained particle’s gravity. The angle θ between the direction of the force and the negative X-axis shows a trend of a “sine curve” shape. When α = 25° and 155°, the angle θ of the force on the entrained particle reaches an extreme value, that is, θ = 21.87°. Only if the angle θ reaches 30º can the particle entrained parallelly escape from the diamond-shaped MPA. Thus, the diamond-shaped MPA remains in a stable state and it is difficult to disperse MPA by changing the direction of the magnetic field.

Association between FGFRs and the susceptibility of digestive and reproductive system cancers in Chinese population

Jia-Kang Wang,Shu-jun Guo,Bao-qing Tian,Chnag-jun Nie,Hai-long Wang,Jia-lang Wang,An Hong,Xiao-jia Chen 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.4

Fibroblast growth factors(FGFs) and their receptors (FGFRs) modulate a wide range of biological functions, especially tumor genesis. The aim of this study was to investigate the possible association of FGFR expression with the susceptibility of digestive system and reproductive system cancers in Chinese population. In total, 343 patients with digestive or reproductive system cancers were enrolled in this study. The expression levels of four highly-conserved FGFRs including FGFR1, FGFR2, FGFR3 and FGFR4 were measured by immunohistochemistry (IHC). The expression levels of FGFRs were compared between carcinoma and para-carcinoma tissues. FGFR1 expression significantly differed between carcinoma and para-carcinoma tissues in colon and gastric cancers. FGFR2 expression significantly differed between carcinoma and para-carcinoma tissues in esophageal cancer. FGFR3 expression was significantly different between carcinoma and para-carcinoma tissues in liver and gastric cancers. FGFR2 showed the highest expression probability in all the selected cancers and FGFR4 showed the lowest expression probability. FGFR1 and FGFR3 showed comparable moderate expression probabilities. Our findings have demonstrated significant differences regarding FGFR expression levels between carcinoma and para-carcinoma cells in digestive or reproductive system cancer patients. The data also implicated that FGFR2 and FGFR4 could serve as two prominent factors closely related to the susceptibility of digestive and reproductive system cancers.

SUNSPOT MOTIONS ASSOCIATED WITH THE 3B/X1.5 SOLAR FLARE OF 13 MAY 1981

WANG JIA LONG,ZHANG GUIQING,MA GUANYI,YUN HONG SIK The Korean Astronomical Society 1996 Journal of The Korean Astronomical Society Vol.29 No.2

We have examined morphological change and movements of individual sunspots within a sunspot group in association with a large solar flare activity (3B/X1.5) appeared on 13 May 1981. For this purpose we measured distance among spots during the period before and after the flare activity and estimated the average velocity of their movement. Our main results are as follows: (1) The longitudinal displacement among sunspots are generally greater than the latitudinal displacement. (2) During the period the spots moved with an average velocity of 1.2 km/s in longitude and 0.86 km/s in latitude. (3) The most notable change took place in the central part placed between the two ribbons of the flare.

박물관 문화상품 디자인 특성이 관광객의 가치인식요인 및 행동의도에 미치는 영향

용가흥(Jia Xing Long),장휘(Hui Zhang) 한국디자인리서치학회 2022 한국디자인리서치 Vol.7 No.4

본 연구의 중국에 박물관 문화상품을 구매한 적이 있는 중국인 관광객을 대상으로 박물관에서 문화상품 디자인 특성이 관광객의 가치인식요인에 어떠한 영향을 주는지, 나아가 박물관 문화상품 산업발전을 위하여 구매의도, 추천의도와 같은 행동의도에 어떠한 영향을 미치는지를 파악하자고 하였다. 이를 위해 온라인 설문조사를 실시였고, 수집된자료는 코딩 과정을 거쳐 자료 분석은 SPSS 24.0과 Amos 25.0을 이용하여 공분산 구조를 확인하고 측정 모델을이용하여 측정항목의 타당성을 확인하였다. 각 변수의 신뢰성, 타당성 검증을 통해 요인을 확보하였다. 이를 바탕으로 도출한 가설을 검증하기 위하여 다수변수 간의 구조적 관계를 확인하기 위하여 Amos 25.0 이용한 경로분석을실시하였다. 연구 결과는 첫째, 박물관 문화상품의 디자인 특성 중에 창의성, 문화전달성이 이용자의 지각적 가치, 브랜드 가치, 기능적 가치에 영향을 미치는 것으로 나타났다. 둘째, 심미성이 지각적 가치에 영향을 미친 것으로 나타나 반면에 브랜드 가치, 기능적 가치에 기각하는 것으로 나타났다. 셋째, 실용성이 브랜드 가치, 기능적 가치에 영향을 미친 것으로 나타나 반면에 지각적 가치에 기각하는 것으로 나타났다. 넷째, 박물관 문화상품의 이용자 가치인식요인이 행동의도에 유의한 영향을 미칠 것이라는 가설을 분석한 결과 채택된 것으로 나타났다. 본 연구가 박물관문화상품 디자인 특성을 통한 관광객의 가치인식을 확보하기 위한 박물관 문화상품 산업 전략 수립의 방안으로써 기초자료가 될 수 있기를 기대한다. The purpose of this study is to investigate how the design characteristics of cultural products in museums affect the perceived value factors of tourists, targeting those who have purchased museum cultural products in China, and furthermore, purchase intention, recommendation intention and We wanted to find out what effect the same behavioral intention had. To this end, an online survey was conducted, the collected data went through a coding process, and for data analysis, the covariance structure was checked using SPSS 24.0 and Amos 25.0, and the validity of the measurement items was confirmed using a measurement model. Factors were secured through reliability and validity verification of each variable. In order to verify the hypothesis derived based on this, a path analysis using Amos 25.0 was conducted to confirm the structural relationship between multiple variables. The results of the study are as follows: First, among the design characteristics of cultural products in museums, creativity and cultural delivery have an effect on users' perceptual value, brand value, and functional value. Second, it was found that aesthetics had an effect on perceptual value, whereas it was found to be rejected by brand value and functional value. Third, it was found that practicality had an effect on brand value and functional value, while rejecting perceptual value. Fourth, as a result of analyzing the hypothesis that the user's perceived value of museum cultural products would have a significant effect on behavioral intention, it was found to be adopted. It is hoped that this study will serve as basic data as a plan to establish a museum cultural product industry strategy to secure the value recognition of tourists through the design characteristics of museum cultural products.