Case Report : Nutrition Therapy for Mitochondrial Neurogastrointestinal Encephalopathy with Homozygous Mutation of the TYMP Gene

( Jing Wang ),( Wei Chen ),( Fang Wang ),( Dong Wu ),( Jia Ming Qian ),( Jun Ren Kang ),( Hai Long Li ),( En Ling Ma ) 한국임상영양학회 2015 Clinical Nutrition Research Vol.4 No.2

Mitochondrial neurogastrointestinal encephalopathy (MNGIE) is characterized by significant gastrointestinal dysmotility. Early and long-term nutritional therapy is highly recommended. We report a case of MNGIE in a patient who was undergoing longterm nutrition therapy. The patient was diagnosed with a serious symptom of fatty liver and hyperlipidemia complications, along with homozygous mutation of the thymidine phosphorylase (TYMP) gene (c.217G > A). To our knowledge, this is the first report of such a case. Herein, we describe preventive measures for the aforementioned complications and mitochondrial disease-specific nutritional therapy.

Pan-cancer Analysis of Tumor Mutational Burden and Homologous Recombination DNA Damage Repair Using Targeted Next-Generation Sequencing

Hai-Yun Wang,Ling Deng,Ying-Qing Li,Xiao Zhang,Ya-Kang Long,Xu Zhang,Yan-Fen Feng,Yuan He,Tao Tang,Xin-Hua Yang,Fang Wang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Current variability in methods for tumor mutational burden (TMB) estimation and reporting demonstrates the urgent need for a homogeneous TMB assessment approach. Here, we compared TMB distributions in different cancer types using two customized targeted panels commonly used in clinical practice. Materials and Methods TMB spectra of 295- and 1021-gene panels in multiple cancer types were compared using targeted next-generation sequencing (NGS). The TMB distributions across a diverse cohort of 2,332 cancer cases were then investigated for their associations with clinical features. Treatment response data were collected for 222 patients who received immune-checkpoint inhibitors (ICIs) and their homologous recombination DNA damage repair (HR-DDR) and programmed death-ligand 1 (PD-L1) expression were additionally assessed and compared with the TMB and response rate. Results The median TMB between gene panels was similar despite a wide range in TMB values. The highest TMB was eight and 10 in patients with squamous cell carcinoma and esophageal carcinoma according to the classification of histopathology and cancer types, respectively. Twenty-three out of 103 patients (22.3%) were HR-DDR–positive and could benefit from ICI therapy; out of those 23 patients, seven patients had high TMB (p=0.004). Additionally, PD-L1 expression was not associated with TMB or treatment response among patients receiving ICIs. Conclusion Targeted NGS assays demonstrated the ability to evaluate TMB in pan-cancer samples as a tool to predict response to ICIs. In addition, TMB integrated with HR-DDR–positive status could be a significant biomarker for predicting ICI response in patients.

Spreading behaviors of high-viscous nanofluid droplets impact on solid surfaces

Hai Long Liu,Xuefeng Shen,RUI WANG,Yuanping Huo,Changfeng Li,Junfeng Wang 한국유변학회 2019 Korea-Australia rheology journal Vol.31 No.3

In this work, the impact dynamics of high-viscous nanofluid droplets onto a solid surface has been investigated experimentally by means of high-speed camera visualization technique. We dispersed various nanoparticles (multiwall carbon nanotube (MWCNT), nano-graphene, and nano-graphite powder) into highviscous base fluid (epoxy resin) to obtain the stable and homogenous nanofluids without surfactant additives. The well dispersed nanofluids show different degree of shear-thinning behaviors, and the shear-thinning properties of those fluids have been characterized by the power-law rheology model. The dynamic contact angle (DCA), transient dimensionless height, and transient contacting factor along with the spreading time under different Weber numbers (We) have been investigated. The results show that the nanofluid with a lower shear viscosity over the entire range of the shear rates results in larger variations of the contacting factor and the dimensionless height. The effect of surface wettability on droplet impact behaviors is more significant for the fluid with higher shear viscosity and less shear-thinning degree during the receding phase. The latter spreading and receding motions of the droplet with higher shear viscosity and shearthinning degree are suppressed significantly, regardless of the Weber numbers in current study. Finally, a model based on experimental data has been proposed to predict the maximum spreading factor of high-viscous droplet impact on solid surface.

Experimental study of nanofluid droplet spreading on a solid substrate

( Hai Long Liu ),( Rui Wang ),( Yuan-ping Huo ),( Jun-feng Wang ) 한국액체미립화학회 2017 한국액체미립화학회 학술강연회 논문집 Vol.2017 No.-

The nanofluid is a class of fluids with high thermal conductivity and non-Newtonian flow behaviors. The spreading characteristics of a nanofluid droplet impinging on the solid surface are the key factors to efficient heat and mass transfer in related processes by the deposition of droplets. In this study, three stable nanofluids (with carbon nanotubes, graphene, nano-graphite powder) were prepared by two-step method. The shear-dependent viscosity of these nanofluids have been studied experimentally. Furthermore, we investigated the evolution morphology of the droplet during the spreading process under various Weber numbers (We) by means of high-speed camera visualization technique. Based on the image processing technique (LB-ADSA), the dynamic contact angle (DCA), rebounding height and spreading factor of the droplet have been obtained along with spreading time. The experimental results show that the degrees of shear thinning in nanofluids are dissimilar and thus influence the spreading behaviors of nanofluid droplet. Specifically, the shear thinning behaviors suppress the spreading and rebounding stage of droplets. This suppression in nanofluid with large infinite shear viscosity are more prominent.

방한 중국 관광객의 설명절 문화 체험 및 재방문 의도에 관한 연구

왕해용(Hai Long Wang),김효정(Hyochung Kim) 한국생활과학회 2012 한국생활과학회 학술대회 Vol.2012 No.11

Heparanase mRNA and Protein Expression Correlates with Clinicopathologic Features of Gastric Cancer Patients: a Meta-analysis

Li, Hai-Long,Gu, Jing,Wu, Jian-Jun,Ma, Chun-Lin,Yang, Ya-Li,Wang, Hu-Ping,Wang, Jing,Wang, Yong,Chen, Che,Wu, Hong-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.18

Background: Heparanase is believed to be involved in gastric carcinogenesis. However, the clinicopathologic features of gastric cancer with high heparanase expression remain unclear. Aim : The purpose of this study was to comprehensively and quantitatively summarize available evidence for the use of heparanase mRNA and protein expression to evaluate the clinicopathological associations in gastric cancer in Asian patients by meta-analysis. Materials and Methods: Relevant articles listed in MEDLINE, CNKI and the Cochrane Library databases up to MARCH 2015 were searched by use of several keywords in electronic databases. A meta-analysis was performed to clarify the impact of heparanase mRNA and protein on clinicopathological parameters in gastric cancer. Combined ORs with 95%CIs were calculated by Revman 5.0, and publication bias testing was performed by stata12.0. Results: A total of 27 studies which included 3,891 gastric cancer patients were combined in the final analysis. When stratifying the studies by the pathological variables of heparanase mRNA expression, the depth of invasion (633 patients) (OR=4.96; 95% CI=2.38-1.37; P<0.0001), lymph node metastasis (639 patients) (OR=6.22; 95%CI=2.70-14.34, P<0.0001), and lymph node metastasis (383 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002) were all significant. When stratifying the studies by the pathological variables of heparanase protein expression, this was the case for depth of invasion (1250 patients) (OR=2.76; 95% CI=1.52-5.03; P=0.0009), lymph node metastasis (1178 patients) (OR=4.79 ; 95% CI=3.37-6.80, P<0.00001), tumor size (727 patients) (OR=2.06 ; 95% CI=1.31-3.23; P=0.002) (OR=2.61; 95% CI=2.09-3.27; P=0.000), and TNM stage (1233 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002). Egger's tests suggested publication bias for depth of invasion, lymph node metastasis, lymph node metastasis and tumor size of heparanase mRNA and protein expression. Conclusions: This meta-analysis suggests that higher heparanase expression in gastric cancer is associated with clinicopathologic features of depth of invasion, lymph node metastasis and TNM stage at mRNA and protein levels, and of tumor size only at the protein level. Egger's tests suggested publication bias for these clinicopathologic features of heparanase mRNA and protein expression, and which may be caused by shortage of relevant studies. As a result, although abundant reports showed heparanase may be associated with clinicopathologic features in gastric cancer, this meta-analysis indicates that more strict studies were needed to evaluate its clinicopathologic significance.

Clinical Study of Thalidomide Combined with Dexamethasone for the Treatment of Elderly Patients with Newly Diagnosed Multiple Myeloma

Chen, Hai-Fei,Li, Zheng-Yang,Tang, Jie-Qing,Shen, Hong-Shi,Cui, Qing-Ya,Ren, Yong-Ya,Qin, Long-Mei,Jin, Ling-Juan,Zhu, Jing-Jing,Wang, Jing,Ding, Jie,Wang, Ke-Yuan,Yu, Zi-Qiang,Wang, Zhao-Yue,Wu, Tian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: To investigate the relationship between the efficacy and safety of different doses of thalidomide (Thal) plus dexamethasone (Dex) as the initial therapy in elderly patients with newly diagnosed multiple myeloma (MM). Methods: Clinical data of 28 elderly patients with newly diagnosed MM who underwent the TD regimen as the initial therapy were analyzed retrospectively. The patients were divided into two groups according to the maximal sustained dose of Thal: lower dose (group A) and higher dose (group B). The overall response rate (ORR), progression free survival (PFS), overall survival (OS), and adverse events (AES) were compared between the two groups. Results: A total of 28 patients were followed up with a median of 18 months. The ORR was 60.1%. The median response time and PFS were 2.0 and 17.0 months, respectively. The mean sustained dose of Thal in group B was significantly higher than group A (292.9 mg v 180.4 mg, P=0.01). There was no significantly difference in ORR (57.1% v 64.3%, P=1.00) and PFS (9.63months v 17.66 months, P=0.73) between groups A and B. During the follow up, only five patients died (<40%) and, therefore, median OS values were not available. It is estimated, however, that the mean survival time in the two groups was 35.6 and 33.4 months (P>0.05), respectively. All of the patients tolerated the treatment well. The incidence of AES in patients with a grading above 3 in group B was significantly higher than in group A (P=0.033). Conclusions: The TD regimen results in a high response rate and manageable AES as the initial therapy in elderly patients with MM. TD should be considered as the front line regimen for the treatment of elderly patients with MM in areas with financial constraints. The clinical response can be achieved at a low dose Thal with minimal toxicity.

Clinical Significance of Upregulation of mir-196a-5p in Gastric Cancer and Enriched KEGG Pathway Analysis of Target Genes

Li, Hai-Long,Xie, Shou-Pin,Yang, Ya-Li,Cheng, Ying-Xia,Zhang, Ying,Wang, Jing,Wang, Yong,Liu, Da-Long,Chen, Zhao-Feng,Zhou, Yong-Ning,Wu, Hong-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Background: miRNAs are relatively recently discovered cancer biomarkers which have important implications for cancer early diagnosis, treatment and estimation of prognosis. Here we focussed on expression of mir-196a-5p in gastric cancer tissues and cell lines so as to analyse its significance for clinicopathologic characteristics and generate enriched KEGG pathways clustered by target genes for exploring its potential roles as a biomarker in gastric cancer. Materials and Methods: The expression of mir-196a-5p in poorly, moderate and well differentiated gastric cancer cell lines compared with GES-1 was detected by RT-qPCR, and the expression of mir-196a-5p in gastric cancer tissues comparing with adjacent non cancer tissues of 58 cases were also assessed by RT-qPCR. Subsequently, an analysis of clinical significance of mir-196a-5p in gastric cancer and enriched KEGG pathways was executed based on the miRWalk prediction database combined with bioinformatics tools DAVID 6.7 and Mirfocus 3.0. Results: RT-qPCR showed that mir-196a-5p was up-regulated in 6 poorly and moderate differentiated gastric cancer cell lines SGC-7901, MKN-45, MKN-28, MGC-803, BGC-823, HGC-27 compared with GES-1, but down-regulated in the highly differentiated gastric cancer cell line AGS. Clinical data indicated mir-196a-5p to beup-regulated in gastric cancer tissues (47/58). Overexpression of mir-196a-5p was associated with more extensive degree of lymph node metastasis and clinical stage (P < 0.05; x2 test). Enriched KEGG pathway analyses of predicted and validated targets in miRWalk combined with DAVID 6.7 and Mirfocus 3.0 showed that the targeted genes regulated by mir-196a-5p were involved in malignancy associated biology. Conclusions: Overexpression of mir-196a-5p is associated with lymph node metastasis and clinical stage, and enriched KEGG pathway analyses showed that targeted genes regulated by mir-196a-5p may contribute to tumorgenesis, suggesting roles as an oncogenic miRNA biomarker in gastric cancer.

Association between FGFRs and the susceptibility of digestive and reproductive system cancers in Chinese population

Jia-Kang Wang,Shu-jun Guo,Bao-qing Tian,Chnag-jun Nie,Hai-long Wang,Jia-lang Wang,An Hong,Xiao-jia Chen 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.4

Fibroblast growth factors(FGFs) and their receptors (FGFRs) modulate a wide range of biological functions, especially tumor genesis. The aim of this study was to investigate the possible association of FGFR expression with the susceptibility of digestive system and reproductive system cancers in Chinese population. In total, 343 patients with digestive or reproductive system cancers were enrolled in this study. The expression levels of four highly-conserved FGFRs including FGFR1, FGFR2, FGFR3 and FGFR4 were measured by immunohistochemistry (IHC). The expression levels of FGFRs were compared between carcinoma and para-carcinoma tissues. FGFR1 expression significantly differed between carcinoma and para-carcinoma tissues in colon and gastric cancers. FGFR2 expression significantly differed between carcinoma and para-carcinoma tissues in esophageal cancer. FGFR3 expression was significantly different between carcinoma and para-carcinoma tissues in liver and gastric cancers. FGFR2 showed the highest expression probability in all the selected cancers and FGFR4 showed the lowest expression probability. FGFR1 and FGFR3 showed comparable moderate expression probabilities. Our findings have demonstrated significant differences regarding FGFR expression levels between carcinoma and para-carcinoma cells in digestive or reproductive system cancer patients. The data also implicated that FGFR2 and FGFR4 could serve as two prominent factors closely related to the susceptibility of digestive and reproductive system cancers.

전후 일본의 국가 대전략과 북일관계

강용범 ( Jiang Long-fan ) , 왕해범 ( Wang Hai-fan ) 조선대학교 동북아문제연구소 2016 동북아연구 Vol.31 No.1

역사적으로 한반도는 일본이 무력으로 지역패권을 도모하고 이른바 “동아시아공영권”의 야망을 실현하는 전초기지가 되어왔다. 제2차세계대전후 일본은 보통국가의 지위와 신분을 회복하고 나아가 아시아지역 강대국으로 부상하는 것을 국가전략의 최종목표로 삼아왔다. 이러한 국가전략을 실현하는 과정에 과거 식민통치에 대한 청산은 비켜설 수 없는 걸림돌이 되었다. 냉전이 한창이던 1965년, 미국의 적극적인 중재하에 한·일양국은 국교정상화를 실현하였다. 따라서 북한과의 관계개선은 전후 일본외교의 중요한 과제로 부상하게 되었다. 일본의 정치가들은 흔히 일본은 전략이 없는 국가라고 한다. 허나 냉전 이후 20여년간 전개되어 온 일본의 대북정책을 살펴보면 일본의 국가전략이 얼마나 교묘한가를 알 수 있다. 우선 북한과의 국교정상화를 통해 정상국가로서의 신분과 지위를 회복하고, 다음으로 북한의 “안전위협”을 빌미로 “군사대국화”를 실현하며, 나아가 6자회담에 끼어들어 한반도다자안보협의에 깊숙이 개입함으로써 정치, 군사 대국화를 위한 기틀을 마련하였다. 뿐만 아니라 일본의 대북정책은 반성과 사죄 그리고 배상이라는 근본적인 문제를 떠나 북한의 핵·미사일과 납치문제에만 집착하고 있다. 더욱이 일본은 중국이나 한국과의 관계가 악화되어 외교적으로 고립될 경우에는 북한과의 관계 완화를 통해 위기를 탈출하려 시도하고, 안보법안과 헌법개정을 위해선 “북한위협론”을 들먹여 제재를 강화하는 등 교묘한 전략을 구사하여왔다. 제4차 북핵실험 후 북·일관계는 또다시 최악의 위기를 맞고 있다. 북한은 일본인납치피해자에 대한특별조사위원회를 해체하였고 일본은 유엔안보리제재는 물론 독자적이고 더욱 강경한 제재를 실시하고 있다. 헌법개정을 본격적으로 추진하고 있는 아베 내각으로 말하면 북한위협이 고조되고 양국관계가 악화되는 것이 더욱 바람직한 전략적 선택일 수도 있다. In history, the Korean peninsula has been an advanced base of Japan to pursue local hegemony through force and realize an ambition to achieve Mutual Prosperity in East Asia. After World War II, Japan has had a final goal of recovering the position and status of a normal country and furthermore, raising its status to one of the top countries in Asia. In the process of realizing such a national strategy, its colonial rule in the past has become a setback which can not be overcome. In 1965 when the Cold War was at its peak period, under the active intervention of the U.S.A, South Korea and Japan normalized diplomatic relations. Therefore, a better relationship with North Korea has become an important task for Japan`s diplomacy before and after the war. Japan`s politicians said that Japan does not have any strategies. However, to look at the policies against North Korea which have been developed for about 20 years after the Cold War, it can be understood how clever Japanese strategies against other countries are. First of all, through normalization of diplomatic relations with North Korea, it recovered the status and position of a normal country. Then, under the pretext of the security threat of North Korea, it realized “Military Power” and furthermore it joined in six-party talks to intervene in the multilateral security talks and were prepared for a great military power. As well, Japan`s policy against North Korea has adhered to nuclear technology ,missiles, and kidnapping out of the fundamental problems of reflection, apology and compensation. In addition, as Japan has had worse relations with China and South Korea, and is isolated diplomatically, it attempted to escape from this crisis through mitigation of relations with North Korea, and for security law and constitution revision, it mentioned the North Korean Threat Issue to reinforce restraint. After the 4th North Korea Nuclear Test, relations between North Korea and Japan faced the worst crisis. North Korea dismantled a special investigation committee into Japanese kidnappings and Japan has implemented more independent and resolute restraints as well as restraints in the U.N. Security Council. To the Abe administration which has actively executed Constitutional reform, it is suggested that the severe threat of North Korea and aggravated relations between the two countries would be a more desirable strategy.