Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

여수국가산업단지 인근 지역 대기오염물질 농도에 영향을 미치는 요인분석

김민영,변정훈,이영훈,이건영,한지은,이예림,임지현,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Eight monitoring stations in the Yeosu National Industrial Complex will determine the concentration of air pollutants measured every hour in 2019, analyze what factors affect them, and how they correlate with the air pollutant measurements. Six air pollutants (SO2, NO2, O3, CO, PM-10 and PM-2.5) measured at eight monitoring stations around Yeosu National Industrial Complex (Seogang-dong, Wolnae-dong, Munsu-dong, Yeocheon-dong, Deokchung-dong, Hwayang-myeon, Yulchon-myeon, Samil-dong) the measurement data for each hour of the year were downloaded to analyze the concentration by weather condition, time zone, season, separation distance from Yeosu Industrial Complex, and separation distance from Gwangyang Industrial Complex. SO2, NO2, and CO concentrations were significantly higher in winter, significantly lower in summer, and O3, PM-10, and PM-2.5 concentrations were significantly higher in spring. SO2, CO, PM-10, and PM-2.5 concentrations were significantly higher between 10 a.m. and 12 p.m., NO2 concentrations were significantly higher between 7 a.m. and 9 a.m., and O3 concentrations were significantly higher between 13 p.m. and 15 p.m.(p<0.001). The concentration of air pollutants (SO2, NO2, CO, PM-10, PM-2.5) excluding O3 was found to have a negative (-) correlation with weather condition, and in the case of O3, it was found to have a positive (+) correlation with PM-10, PM-2.5, temperature and wind speed. It was confirmed that the concentration of air pollutants emitted from the Yeosu National Industrial Complex differs depending on the season, time and distance to the industrial complex. This study aims to establish measures to minimize health damage to residents living relatively close to the industrial complex and contribute to the establishment of management and response measures according to the properties of substances to reduce the concentration of air pollutants in the future.

근피로 후 세 가지 회복 방법

이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

전신홍반루푸스 환자에서 Corticosteroids 복용 중에 B형 간염이 활성화한 1예

곽지영 외 중앙대학교 의과대학 의과학연구소 2011 中央醫大誌 Vol.36 No.3/4

Hepatitis B virus (HBV) infection is endemic in Asia and reactivation of HBV is a well recognized complication in infected patients who undergo cytotoxic chemotherapy for cancer and immunosuppressive therapy. Since immunosuppressant can facilitate viral replication and lead to reactivation of HBV, the management of systemic lupus erythematosus (SLE) patients who are HBsAg-positive presents a clinical challenge. We report here on a 29-year old female with SLE and HBsAg positivity who presented hepatitic flare during corticosteroid treatment only. HBsAg carriers who receive cytotoxic or immunosuppressive therapy are at considerable risk of reactivation of HBV infection. Antiviral prophylaxis is clinically efficacious as primary prophylaxis to prevent HBV reactivation. Risk factors allowing patient stratification for HBV reactivation include foremost the HBV DNA level before initiation of immunosuppresive treatment, including corticosteroids. Monitoring for development of acute flare of HBV should be performed by serial measurement of HBV DNA and liver enzymes regularly.

車體構造解析硏究

김지홍,김천욱,이신영 연세대학교 산업기술연구소 1988 논문집 Vol.20 No.1

An analysis on the load-bearing capacity of floor frame and body structure is investigated by the three dimensional rigid frame analysis. The load-bearing capacity of the body structure is estimated on the basis of comparison to the floor-frame-only structure. The location and magnitude of the maximum displacement are also determined and show good agreement with those of a previous study.In the present study the load-bearing capacity of the floor frame of van-type structures is about 80 percent of the total loadings.

텔레매틱스용 광대역 LNA 설계 및 제작

김지윤,양두영 제주대학교 공과대학 첨단기술연구소 2005 尖端技術硏究所論文集 Vol.16 No.2

In this paper, the wideband low noise amplifier for telematics terminals is proposed. In order to stabilize the input and output VSWR and minimize the noise level over wide frequency ranges, the balanced configuration and double open-stub matching circuit are adaptive. And to obtain high gain over 30dB, two amplifiers composed of MGA-86563 PHEMT are connected in cascade. From the results, the proposed low noise amplifier has flat gain over 0.8-2.6GHz, low VSWR and minimum noise figure in the desired bandwidth. Total gain of LNA is 35dB and noise figure and VSWR are 2.5dB and 1.3, respectively.

YCbCr 공간에서의 컬러 영역화

김지영 서원대학교 미래창조연구소 2004 과학과 문화 Vol.1 No.1

In this paper a new color segmentation algorithm is proposed. The proposed algorithm uses YCbCr 3D color space where similar group of colors are easily identified. Two dimensional color space has difficulty in identifying group of colors while the processing time is surely advantageous. In the YCbCr histogram, those groups of colors are looked like stems and branches of a tree. A recursive region growing algorithm is developed and implemented in this paper. The proposed algorithm gives good results but the processing cost is very high. The spatial information of the image plane for the proposed region growing algorithm will be useful for the future research.

점포내 VMD요소가 SPA 브랜드 정체성에 미치는 영향

김소영, 손지훈, 심효진, 신동훈, 정일호, 홍동우, 김은영 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

This study was to examine the effect of VMD factors on brand identity for SPA brands. As brand stimulus, four SPA brands(e.g., Basic House, Codes combine, Uniqlo, Zara) were selected by pretest. For collecting data, a self-administered questionnaire was developed based on the literatures and intercept survey was conducted. A total 508 usable responses were obtained from consumers who shopped at the selected brands' stores. For data analysis, descriptive statistics, factor analysis, and regression analysis were used. As perceived by consumers, VMD consisted of seven factors: lightening, scent, color, layout, music, window display, and item presentation. The VMD factor was significantly related to brand identity for the SPA brands. Specifically, brand awareness was predicted by color, layout and item presentation; brand association strength was predicted by color, layout, and widow display; favorable brand association was predicted by item presentation; salience of brand association was predicted by color and music; and brand image association was predicted by color, layout, music, and item presentation. Thus, this study discussed a managerial implication for SPA retailers to develop a visual merchandising strategy in the competitive marketplace.

전산전문 교육의 현황 및 추세

김지영 서원대학교 미래창조연구소 2004 과학과 문화 Vol.1 No.1

Since the advent of computers to current computer ubiquity, the efforts to educate the computer specialists to serve the needs of our society and the field, have been ever changing and expanding. This article traces the trend of computer education, more broadly, information technology education in general and at the college level. The progress of computer development is divided into several stages and the corresponding adaptation of the computer education is presented: From the time that computer not as an independent academic field, to an era that almost all disciplines are pervasively related to computers in one way or another.