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      • 김치식이가 흰쥐의 항산화시스템에 미치는 영향

        류승희,권명자,송영옥,송영선,문갑순 인제대학교 2003 仁濟論叢 Vol.18 No.1

        김치는 배추를 주재료로 하여 마늘, 고추, 생강 등의 양념류를 포함하는 여러 재료들로 만들어지며 이들 재료 중에는 항산화효과를 가진 것으로 알려진 성분들이 많이 함유되어 있어 김치도 항산화효과를 나타낼 것으로 여겨진다. In vitro 실험계 및 세포 모델계에서 김치 재료 및 김치의 항산화효과는 확인된바 있으나 In vivo 시스템에서의 김치의 항산화효과에 관한 자료는 부족하다. 따라서 본 실험에서는 김치 섭취의 생체 내 항산화효과를 규명하기 위하여 흰쥐에게 동결건조한 김치 3, 5, 10% 첨가 식이를 6주간 섭취시키면서 간조직의 산화와 항산화효소계의 활성을 측정하였다. 간의 MDA 함량은 대조군에 비해 김치 투여군에서 낮았고 특히 5% 김치섭취군에서 현저히 낮은 값을 나타내었다. 단백질 산화를 나타내는 카르보닐 함량도 대조군에 비해 김치섭취군에서 낮아 생체 내에서 김치식이가 과산화물 생성 억제효과를 나타내었다. 항산화 효소계의 활성을 측정한 결과 SOD 활성은 대조군에 비해 김치섭취군에서 현저하게 높았고 김치첨가 함량이 증가할수록 SOD 활성이 유의적으로 높아져 10% 김치섭취군에서는 대조군에 비해 70% 이상 높은 활성을 나타내었다. Catalase 활성은 각 군에서 유의적인 차이가 나타나지 않았으나 GSH-px의 활성은 김치 섭취가 높을수록 상대적으로 높은 증가율을 나타내어 SOD 활성과 동일한 경향을 나타내었다. 따라서 김치식이는 생체 내에서 치질 및 단백질 과산화를 현저하게 억제하였고 항산화효소계인 SOD와 GSH-px의 활성을 농도의존적으로 증진시켜 김치식이가 체내의 산화적 스트레스를 효과적으로 억제하는데 기여함을 알 수 있었다. Kimchi is a Kruean traditional fermented food containing various ingredients such as Chinese cabbage, garlic, ginger, red pepper and fish sauce. Some of their components were known as its antioxidative activities and antioxidative effects of kimchi have been studied using in vitro and cell model system. To investigate the antioxidative effect of dietary kimchi, 4 groups of male S.D. rats (n=36) were fed 0, 3%, 5%, and 10% freeze-dried kimchi for 6 weeks and hepatic lipid peroxidation, protein oxidation and the activities of antioxidative enzymes were measured. Hepatic MDA content fed kimchi diet was lower than control diet. Protein carbonyl values were decreased in kimchi diet group, but not significant. SOD activities were significantly increased in kimchi added groups at dose-dependent manner. At 10% kimchi added group, SOD activity increased by 70% compared with control diet group. Catalase activities were not different between experimental groups and control group, whereas GSH-px activities were significantly increased in kimchi added group. As a result kimchi diet inhibited lipid and protein oxidation and induced antioxidative enzymes, SOD and GSH-px in rat hepatic tissue. It suggests that dietary kimchi is expected to prevent the oxidative stress in animal and human.

      • 1971年度 韓國에서 分離 된 살모넬라 菌屬의 同定에 關한 報告

        柳榮海,金英子 대한감염학회 1972 감염 Vol.4 No.1

        The authors identified 176 Salmonella cultures either isolated from the apparent cases in various epidemic areas or collected from the Seoul infectious diseases hospital, the provincial hygiene laboratories and the county health centers in order to be confirmed bacteriologically during the period from January to December in 1971 since the preparation for establishing Korean National Salmonella Center was on the pavement to provide the diagnostic service over the country. The screening procedures for the morphological and biochemical tests, and the serological determinations for the somatic and flagellar antigens as well as Vi antigen were performed according to the recommendations made by U.S. Center for Disease Control. The results of the laboratory tests were summarized as follows: 1. Of 176 Salmonella cultures, 163 Salmonella typhi with Vi antigen and 13 other Salmonella cultures were confirmed. 2. With 163 S. typhi cultures, all typically showed methyl red positive, motility positive, acid from glucose positive, mannitol positive. 9.2% showed sorbitol positive, 0.6% showed arabinose positive, 61.4% showed arginine dihydrolase positive and 77.3% showed D-tartrate positive as summarized in Table 1. 3. With 13 Salmonella cultures other than S. typhi, 92.3% showed Simmons' citrate positive, and all produced acid and gas from glucose. All showed sorbitol positive, 84.6% showed dulcitol positive, 23.1% showed inositol positive, 92.3% showed arabinose positive, 69.3% showed rhamnose positive, 15.4% showed mucate positive, and all showed lysine decarboxylase and arginine dihyrolase positive as summarized in Table 1, too. 4. Of 13 Salmonella cultures other than S. typhi, one S. paratyphi A, one S. typhimurium, one S. jericho, one S. colorado, one S. bareilly, three S. berta and five S. enteritidis cultures were confirmed as shown in Table 2 and S. jericho was considered not to have ever been reported in the country before. 5. The sensitivity tests, by means of Ericsson's disc method, to eight kinds of antibiotics were carried out, i.e. chloramphenicol, neomycin, erythromycin, colistin, kanamycin, tetracycline, streptomycin, and ampicillin, which were widely in common use in Korea and the result were compared with that of Salmonella cultures isolated during the period from 1967 to 1970 as shown in Table 3. There were three cultures resistant to chloramphenicol and 27 cultures (17%) resistant to ampicillin found among 163 cultures of S. typhi. With 13 cultures of Salmonella other than S. typhi, five cultures were found to be resistant to ampicillin, but none to chloramphenicol. 6. In regards to the multiple resistance to the antibiotics tested, with 163 cultures of S. typhi, all cultures tested showed multiple resistant patterns to more than three kinds of antibiotics in various combinations as shown in Table 4. 152 cultures(ca 93%) showed multiple resistant patterns to more than four kinds of antibiotics in various combinations. 128 cultures (79%) showed that to more than five kinds of antibiotics and 33 cultures (18%) showed that to more than six kinds of antibiotics in various combinations including chloramphenicol. And the results were also compared with the results obtained from the previous study during the period from 1967 to 1970 as shown in Table 4.

      • 비지의 영양학적 품질특성

        김군자,류호경,이영근,김동섭 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

        This study was carried out to examine the qualities of soybean curd residue for utilizing as foodstuff. The content of moisture, crude protein, crude fat and ash were 80.98, 3.32, 0.94 and 0.82%, respectively. The major fatty acids were linoleic acid, oleic acid, linolenic acid and stearic acid. The hydrolysate of protein of sobean curd residue was composed chiefly of aspartic acid, glutamic acid, glycine, alanine, arginine and threonine, but the major free amino acids of soybean curd residue were arginine(234.1), ethanolamine (91.6), γ -amino-n-butyric acid(60.2), glutamic acid(55.8), valine(49.8), asparagine(47.3) and Iycine(48.3μ mol/100g). Key words : Soybean curd residue, Fatty acid, Amino acid

      • 간장의 덧에서 분리한 Zygosaccharomyces rouxii BH-90과 Candida versatilies BH-91의 동정및 특성

        조경자,박춘옥,채영주,김동석,류병호 慶星大學校 1993 論文集 Vol.14 No.2

        This studies attempted to bred the two yeasts of highest level of alcohol and 4-ethylguaiacol production from fermented mash conventional soy sauce. Two kinds of yeast isolated from conventional fermented mash were identified and designated as Zugosaccharomyces rouxii BH-90 and candida versatilis BH-91 which produced the highest levels of ethyl alcohol and 4-ethylguaiacol. The two yeast strains preferred pH 5.0 and 30℃for highest levels of 2.5% ethylalcohol and 18mg/? 4-ethylgaicacol production.

      • 消費者의 衣服購買時 品質表示 制度의 認識度와 表示內容의 實態調査

        殷英子,柳德桓,咸玉相 啓明大學校 生活科學硏究所 1988 科學論集 Vol.14 No.-

        The purpose of this study was to investigate the purchasing pattern toward the garment's label and to find the problem of content's label. The questionnair method was used to obtained the data which was made by a result of self-administered questionnair, the lot of random sample for this paper was 390 subgects and the problem in the content's label was contemplate as the results of chemical method. The results indicate that; 1. The young's class, high scholarship and the small income earners had the more interest on the ready made clothes than the case of the contrary. 2. The group of 40∼50 year's old had the most interest in the garment label and the second case was the large income earner's and high scholarship's group. 3. An item of the interest in the label was the price and the size, everyone had understood generally the label contents, especially group of young's years all more than old year's, and the mark of Korean standard a little more than international standard's. 4. Percentages of the fibre blending were incorrected, especially mark's term was out of keeping by reason of the a bombastic advertisment.

      • SCOPUSKCI등재

        A Case of Paraneoplastic Limbic Encephalitis Associated with Small Cell Lung Cancer

        Ryu, Ja Young,Lee, Seung Hyeun,Lee, Eun Joo,Min, Kyung Hoon,Hur, Gyu Young,Lee, Sung Yong,Kim, Je Hyeong,Lee, Sang Yeub,Shin, Chol,Shim, Jae Jeong,In, Kwang Ho,Kang, Kyung Ho,Yoo, Se Hwa The Korean Academy of Tuberculosis and Respiratory 2012 Tuberculosis and Respiratory Diseases Vol.73 No.5

        Paraneoplastic limbic encephalitis (PLE) is a rare syndrome characterized by memory impairment, affective and behavioral disturbances and seizures. Among many different neoplasms known to cause PLE, small cell lung cancer (SCLC) is the most frequently reported. The pathogenesis is not fully understood but is believed to be autoimmune-related. We experienced a patient with typical clinical features of PLE. A 67-year-old man presented with seizure and disorientation. Brain magnetic resonance imaging demonstrated high signal intensity in the bilateral amygdala and hippocampus in flair and T2-weighted images suggestive of limbic encephalitis. Cerebrospinal fluid tapping revealed no evidence of malignant cells or infection. Positron emission tomography/computed tomography showed a lung mass with pleural effusion and a consequent biopsy confirmed the diagnosis of PLE associated with SCLC. The patient was subsequently treated with chemotherapy and neurologic symptoms gradually improved.

      • SCOPUSKCI등재

        Vitrification of mouse embryos using the thin plastic strip method

        Ryu, Eun Kyung,Hur, Yong Soo,Ann, Ji Young,Maeng, Ja Young,Park, Miji,Park, Jeong Hyun,Yoon, Jung,Yoon, San Hyun,Hur, Chang Young,Lee, Won Don,Lim, Jin Ho The Korean Society for Reproductive Medicine 2012 Clinical and Experimental Reproductive Medicine Vol.39 No.4

        Objective: The aim of this study was to compare vitrification optimization of mouse embryos using electron microscopy (EM) grid, cryotop, and thin plastic strip (TPS) containers by evaluating developmental competence and apoptosis rates. Methods: Mouse embryos were obtained from superovulated mice. Mouse cleavage-stage, expanded, hatching-stage, and hatched-stage embryos were cryopreserved in EM grid, cryotop, and TPS containers by vitrification in 15% ethylene glycol, 15% dimethylsulfoxide, 10 ${\mu}g/mL$ Ficoll, and 0.65 M sucrose, and 20% serum substitute supplement (SSS) with basal medium, respectively. For the three groups in which the embryos were thawed in the EM grid, cryotop, and TPS containers, the thawing solution consisted of 0.25 M sucrose, 0.125 M sucrose, and 20% SSS with basal medium, respectively. Rates of survival, re-expansion, reaching the hatched stage, and apoptosis after thawing were compared among the three groups. Results: Developmental competence after thawing of vitrified expanded and hatching-stage blastocysts using cryotop and TPS methods were significantly higher than survival using the EM grid (p<0.05). Also, apoptosis positive nuclei rates after thawing of vitrified expanded blastocysts using cryotop and TPS were significantly lower than when using the EM grid (p<0.05). Conclusion: The TPS vitrification method has the advantages of achieving a high developmental ability and effective preservation.

      • KCI등재

        A Case of Paraneoplastic Limbic Encephalitis Associated with Small Cell Lung Cancer

        Ja Young Ryu,Seung Hyeun Lee,Eun Joo Lee,Kyung Hoon Min,Gyu Young Hur,Sung Yong Lee,Je Hyeong Kim,Sang Yeub Lee,Chol Shin,Jae Jeong Shim,Kwang Ho In,Kyung Ho Kang,Se Hwa Yoo 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.73 No.5

        Paraneoplastic limbic encephalitis (PLE) is a rare syndrome characterized by memory impairment, affective and behavioral disturbances and seizures. Among many different neoplasms known to cause PLE, small cell lung cancer (SCLC) is the most frequently reported. The pathogenesis is not fully understood but is believed to be autoimmune-related. We experienced a patient with typical clinical features of PLE. A 67-year-old man presented with seizure and disorientation. Brain magnetic resonance imaging demonstrated high signal intensity in the bilateral amygdala and hippocampus in flair and T2-weighted images suggestive of limbic encephalitis. Cerebrospinal fluid tapping revealed no evidence of malignant cells or infection. Positron emission tomography/computed tomography showed a lung mass with pleural effusion and a consequent biopsy confirmed the diagnosis of PLE associated with SCLC. The patient was subsequently treated with chemotherapy and neurologic symptoms gradually improved.

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