Thiuram계 화물합들의 가황촉진 효과에 관한 연구

나혜복,이명환,박인수 서울여자대학교 1979 서울여자대학논문집 Vol.- No.8

Three thiuram disulfide derivatives which could be used as vulcanizing accelerators 〔tetramethyl thiuram disulfide(TMDM), tetraethyl thiuram disulfide(TETD), and tetra-n-butyl thiuram disulfide(TBTD)〕were prepared by oxidizing the corresponding dithiocarbamates with hydrogen peroxide-sulfuric acid or chlorine. Tetramethyl thiuram monosulfide(TMTM) was also prepared by the reaction of the corresponding disulfide with potassium cyanide. The average particle size of TMTD was about 1.0∼2.2μ. The particle size of TMTD obtained from hydrogen peroxide-sulfuric acid oxidation was somewhat smaller than that from chlorine oxidation. The effect of smaller particle size showed a little better tensile strength and elongation properties in the vulcanized rubber. By applying these thiuram disulfide accelerators to the natural rubber, we found that the scorch time became speedy when the carbon number diminished in N-alkyl substituents. When the natural rubber was vulcanized with a mixture of TMTM 〔2.0 part per hundred recipe (phr)〕and sulfur (0.75phr), the physical properties of the cured rubber were same as those vulcanized with TMTD.

Monocrotaline투여 백서 폐동맥고혈압에서 폐혈관내피세포와 폐신경내분비세포의 변화에 대한 형태학적 연구

양우익,조상호,최인준,이유복 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.6

일반 카메라를 이용한 지상 사진 측정의 공간좌표 해석에 관한 연구

유복모,강인준,이광호 연세대학교 산업기술연구소 1981 논문집 Vol.13 No.1

The field of terrestrial photogrammetry is used for the displacement of the structures, investigating cultural assets and traffic accidents which is difficult by aerial photogrammetry. Analyzing with non-metric 35mm camera is also successfully employed to study the accuracy and economic problem. This paper measured the angle of vertical and horizontal with transit, and the object had taken by a combination of non-metric camera and transit. As a result of this study, using the analysis of grid by non-metric 35mm camera can be highly recommended for the consistent application of terrestrial photogrammetry.

최근 10 년간 상부소화관내시경으로 진단된 소화성궤양의 변모

김경희,이상인,이은경,강진경,박인서,전재윤,문영명,정재복,최흥재,임대순 대한내과학회 1987 대한내과학회지 Vol.32 No.1

In this century, the very striking changes in incidence and prevalence of duodenal ulcers, as well as the changes in proportion of males to females, in the age of the affected populations and in the proportion of duodenal to gastric ulcers had occurred and these epidemiological changes are ongoing. Therefore, 5,851 cases with endoscopically proven peptic ulcers were reviewed from Jan. 1976 to Dec. 1985. The results obtained are as follows: 1) During the period 1976-1985, the proportion of gastric ulcer among peptic ulcer disease decreased from 71.8% to 43.6%, but that of duodenal ulcer increased from 24.2% to 47.9%. 2) In peptic ulcer disease the male to female ratio was declined from 4.5:1 in 1976 to 3.4:1 in 1985. 3) Mean ages of patients with gastric or duodenal ulcer were 49.4±13.0 or 40.0±12.5, respectively, and there were significant differences between two groups (p$lt;0.005). 4) Hospitalization rates for peptic ulcer, particularly duodenal ulcer, had fallen from 51.5% in 1976 to 35.3% in 1985. 5) Peptic ulcer disease, particularly duodenal ulcer, was less frequent in August. 6) There was no difference between the mean age of solitary ulcer patients and that of multiple ulcer patients. In summary, daring the period 1975-1986, the proportion of duodenal ulcer among peptic ulcer disease was increased, peptic ulcer disease in women was increased, but the hospitalization rates were decreased.

식도암의 심달도 판정에 있어서 내시경적 초음파검사의 유용성

이상길,박승우,강진경,박인서,정재복,송시영,이용찬,문영명 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2

Background: Despite the technical developments in diagnosis and therapy, esophageal cancer is highly lethal disease and the survival is largely dependent upon the stage of the disease. Preoperative cancer staging is crucial in choosing a therapeutic option as well as in predicting the prognosis of the patients. Staging has been based on computerized tomography (CT) and transabdominal ultrasonography. However CT has a limit in pre-cisely discriminating the depth of invasion or the lymph node metastases. With the devel-opment of endoscopic ultrasonography (EUS) and with its superiority in delineating wall structure and detecting lymph node metastases, its usefulness in staging for esophageal cancer has been cknowledged. In order to evaluate the accuracy of EUS, we compared EUS with pathologic findings in patients with esophageal carcinoma. Methods: From July 1990 to August 1997, 136 patients with esophageal cancer received preoperative cancer staging with EUS. Among them, 48 patients who underwent surgical procedures with the intention of radical resection were included. We compared the EUS and pathologic find-ings and analysed the accuracy of EUS for preoperative staging. Results: The overall accu-racy of EUS for T-staging was 43.8%. Twenty five percents of the patients (12/48) pre-sented high-grade tumor strictures, which precluded the passage of the endoscope. There was no statistical significance according to tumor site, size or gross morphology. However theaccuracy was significantly lower in tumors with ulceration than in tumors without ulceration (35.3% vs 64.3%, p=0.004). Mainly, ulceration in tumors caused significant overstaging of the T-stage. In the assessment of regional lymph node metastasis, the overall accuracy achieved by EUS was 66.6%; the sensitivity was 95.5%, specificity 42.3%, positive predictive value 58.3%, and negative predictive value 91.7%. Tumors with more than 2 lymph nodes rendered more accurate N-staging than tumors with less than 2 lymph nodes. Conclusions: In conclusion, the accuracy of the EUS for preoperative staging of esophageal cancer was not satisfactory, mostly influenced by ulceration in tumors and its resultant inflammatory reactions around the tumors, therefore more systematic study will be needed to establish the precise diagnostic criteria of EUS staging.

각종 간질환에 있어서 이상 Prothrombin (des-γ-Carboxyprothrombin)에 관한 연구

이혁우,정경섭,김원호,한광협,정재복,이상인,박인서,최홍재,도윤정,윤홍섭 대한내과학회 1990 대한내과학회지 Vol.38 No.4

The absence of vitamin K or the ingestion of vitamin K antagonists inhibits vitamin K-dependent carboxylase activity in the liver, and an abnormal prothrombin, known as des-y-carboxyprothrombin(DCP) or PIVKA -Ⅱ(a protein induced by vitamin K absence or antagonists-Ⅱ), is released into the blood. In order to evaluate whether abnormal prothrombin levels can be clinically used as an index of hepatocellular dysfunction or as a tumor marker of hepatocellular carcinoma(HHC), DCP levels were determined by a latex agglutination test in 20 normal subjects and in patients with various liver diseases, including 70 hepatocellular carcinoma, sever metastatic liver disease, 45 liver cirrhosis, 13 acute viral hepatitisB, six chronic active hepatitis B, three fatty liver and one liver abscess. The usefulness of the combination assay of DCP and alpha-fetoprotein(AFP) levels to improve the diagnostic value and the effects of vitamin K administration on DCP levels were assessed in the present study. The results obtained were as follows: 1) DCP was detected in 42 out of 70 patients with HCC(60.0%), in three seven patients with metastatic liver disease(42.9%), in 23 out of 45 patients with liver cirrhosis(51.1%), and in one out of six patients with chronic active hepatitisb(16.7%), but there was no detectable DCP among the 20 healthy control subjects or in the 13 acute viral hepatitis B, three fatty liver and one liver abscess cases. 2) The detection rates of DCP according to the size of the HCC were 66.7% in the larger-than 5㎝ size and 44.4% in the 3~5㎝ size, but there was no detection in four patients with smaller-than 3㎝ size. The detection rates of DCP according to Child’s classification of liver cirrhosis were 60% in class C and 25% in class B, but there was no detection in two patients in class A. 3) There was no significant correlation between DCP and AFP levels. However, DCP was also detected 62.5% in less than 400ng/㎖ of AFP, and the positive rates were 91.0% in higher than 400ng/㎖ of AFP or higher than 1:10(+) of DCP in patients with HCC. 4) The detection rate of DCP was 56.8% in liver cirrhosis patients with prolonged prothrombin time(PT). However, 61.4% was detected in HCC patients with normal levels of prothrombin time(PT). 5) On observation of the effectiveness of vitamin K administration on DCP level, are was no effectiveness of vitamin K administration in all patients with 13 HCC, but the DCP level decreased or was not detected in seven out of nine patients with liver cirrhosis after vitamin K administration (p<0.05). Based on these results, DCP determined by a latex agglutination test may be useful as an index of hepatocellular dysfunction. However, due to the lower sensitivity and specificity of the latex agglutination test, it is doubtful whether DCP is a definite tumor marker of HCC. But the combination assay of AFP and DCP is helpful for obtaining an increased diagnostic rate of HCC. We recommended comparison of the effectiveness of vitamin K administration on DCP concentrations during the follow-up observation of chronic liver disease, such as liver cirrhosis, for increasing the diagnostic rate of HCC. Further study utilizing methods such as RIA or ELISA might be needed to evaluate the usefulness of DCP as a tumor marker for HCC.

췌장암에서 p53 단백발현의 임상적 의의

박인서(In Suh Park),정재복(Jae Bock Chung),박찬일(Chan Il Park),송시영(Si Young Song),이돈행(Don Haeng Lee),이우정(Woo Jung Lee),박영년(Young Nyun Park) 대한소화기학회 1997 대한소화기학회지 Vol.30 No.1

N/A Background/Aims: Alteration in the p53 tumor suppressor gene are involved in the pathogenesis of diverse human cancer. Immunohistochemical detection of the p53 protein has been strongly correlated with rnutations in the p53 gene. Although p53 overexpression or mutation have been elucidated in a variety of cancers, there has been no study in pancreatic cancer in Korea. The purpose of this study is to evaluate the expression rate of p53 protein in pancreatic cancer. In addition, we attempted to clarify the association between expression of p53 and clinicopathologic features including survival. Methods: Using immunohistochemistry with monoclonal antibody to p53(Zymed Lab. Clone No. BP53-12), we exained 47 formalin-fixed paraffin-embedded tissues of pancreatic cancer(39 intraductal adenocarcinoma, 8 rnucinous adenocarcinoma) for overexpres- sion of p53 gene product. Results: Positive nuclear p53 immunoreativity was detected in 44.6% of pancreatic cancer. No clear correlation was found between p53-positive immunostaining and clinicopathologic features such as age, sex, tumor size, location, histologic type, differentiation of tumor cells and stage of tumnor, etc. The mean duration of survival was 8.6 months in the group of p53 positive and 9.6 months in the group of p53 negative and no difference was noted between two groups. Conclusions: These results suggest that mutation of p53 tumor suppressor gene may play a role in the pathogenesis of pancreatic cancer. However there was no correlation between the presence of p53 overexpression and clinicopathologic features or survival. (Korean J Gastroenterol 1997; 30:98 - 102)

간질환 환자의 갑상선 기능

이상인(Sang In Lee),조준구(Jun Koo Cho),전재윤(Chae Yoon Chon),함기백(Ki Baek Ham),정재복(Jae Bock Chung),최흥재(Heung Jai Choi) 대한내과학회 1988 대한내과학회지 Vol.34 No.3

N/A The liver has been recognized as an important site in the peripheral metabolism of thyroid homone, particularly in the mondeiodination of thyroxine to triiodothyronine. It is also the source of serum T, binding proteins. As is to be expected, disease of the liver has marked effects on serum thyroid hormone concentrations and bindings. In order to evaluate the change of thyroid hormones in various liver diseases and to elucidate the relationship between thyroid hormone status and severity of liver diseases, both thyroid hormones and liver functions were measured and analyzed in 110 patients with various liver diseases who were admitted to Yonsei University Severance Hospital during the period from April 1986 to October 1986. The results were as follows: 1) In various liver disease, acute hepatitis, fatty liver, chronic persistent hepatitis and chronic active hepatitis showed no significant difference in serum T3; T4, FT4, TSH and T3/T4 compared to normal controls. 2) The serum T3, T4 and FT4 of liver cirrhosis were signficantly lower than those of control group. but serum TSH, T3/T4 were within normal limits in liver cirrhosis. 3) The serum T3 and T4 and of hepatoma was significantly lower than those of control group, but mean serum FTTSH and T3/T4 showed no significant difference between hepatoma and control group. 4) The mean serum T3 level of seven patients with Child's class A was 100.73±25.44ng/dl, thirteen patients with class B 76.42±14.40ng/dl, and eighteen patients of class C 65.18±23.19ng/dl. There was a significant statistical difference among Child's class A, B and C. 5) There was no significant correlation between serum T3 and liver functions in Child's class A of liver cirrhosis. Serum T3 was significantly correlated with-total protein and albumin in Child's class B, with total protein, albumin and prothrombin time in Child class's C. 6) In liver cirrhosis, low serum T3 group (T3 <80 ng/dl) had a significant decrease in total protein, albumin and prolonged prothrombin time than normal serum T3 group (80 ng/dl < T3 < 220 ng/dl). 7) Serum T3 decreased significanlty in liver cirrhosis with ascites than in those without ascites. In conclusion, in liver cirrhosis and hepatoma, significant decrease in serum T3 and T4 level were observed and particularly serum T3 level may be useful in assessing the severity of hepato-cellular damage in liver cirrhosis.

원발성 위 림프종의 내시경적 진단

강진경,박인서,문영명,정재복,이세준,양우익,박찬일,송시영,이명래 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.2

Primary gastric lymphorna represents one to 7% of all gastric cancer and is the most common type of extranodal lymphoma. In attempt to evaluate the endoscopic characteristics, we analysed clinical and endoscopic findings in 35 patients with primary gastric lymphoma between January 1980 and August 1994 at the Yonsei Medical Center. The results were as follows: 1) The mean age of patients was 47.1 years with male to female ratio of 1.5:1. 2) Gastroscopy was performed in all 35 patienits, which revealed polypoid lesion in one case(2.9%), ulcerative lesion in 15 cases(42.9%), ulcero-infiltrative lesion in 6 cases(17.1%) and diffuse infiltrative lesion in 2 cases(5.7%). Gastric lymphoma was suggested in 6 cases, advanced gastric cancer in 21 cases, early gastric cancer in 5 cases and benign gastric ulcer in 3 cases. Pathologic diagnosis of biopsy specimens were gastric lymphoma in 24 cases, adenocarcinoma in 5 cases and chronic superficial gastritis in one case. 3) The characteristics of the endoscopic findings in gastric lymphoma were intractable or recurrent ulcer in 10 cases, thickened and mounded ulcer margin in 9 cases, multiple ulcers in 9 cases, giant rugae in 7 cases and polypoid or depressed lesion with central ulceration in 4 cases. In conclusion, recognition of specific endoscopic findings such as intractable or recurrent ulcer, volcano-like ulcer, multiple ulcers, giant rugae and combined lesion, the possibility of a lymphoma should be considered and vigorous biopsy attempts should be carried out. Then if the first microscopic report does not suggest this diagnosis, a second investigation including jumbo biopsies, perhaps by diathermy, should be undertaken.

근치적절제술을 시행한 췌장암환자의 예후인자로서 Flow Cytometry를 이용한 DNA Ploidy 검사의 의의

이은섭(Eun Sub Lee),송시영(Si Young Song),정재복(Jae Bock Chung),박영년(Young Nyun Park),강진경(Jin Kyung Kang),박인서(In Suh Park),박찬일(Chan Il Park) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.2

N/A Background/Aims: Only about 5 l0% of all the patients with pancreatic carcinoma that under- went radical resection survive for more than five years. Various factors related to the prognosis arc presently known, such as size, location, stage, multicentricity, cell type, histologic grading, mitotic activity, type of surgery, intraoperative irradiation, performance status, sex, etc. Recently DNA analysis using flow cytometry has widely been used to evaluate the prognostic factors in various malignancies, and there appears to be a relationship between DNA aneuploid and decreased survi- val. However the results are still controversial. Thus we conducted this study to evaluate the prognostic effect of DNA ploidy and synthetic phase fraction in patients with pancreatic cancer who underwent radical operation. Methods: Flow cytometric DNA analysis was performed using formalin fixed paraffin-embedded specimens of 14 cases of pancreatic carcinoma that underwent radical resection. In addition, clinicopathological review was done to determine the usefulness of this technique in predicting biologic behavior and prognosis. Results: Aneuploidy was noted in 6 cases(4'2.9/c) and diploidy in 8 cases(57.1%). The S-phase fraction(SPF) in aneuploid tumor(23.7 /n) was significantly higher than that in diploid tumor(9.5%). The median survival was 32.9 months and l5.7 months in diploid and aneuploid tumor respectively, and 44.3 months and 20.1 months in S-phase fraction below and above median value(l 1.0%) respectively. Aneuploidy and higher S-phase fraction were frequently encountered in cases with recurrence within 24 months after operation than in cases without recurrence during the entire follow-up period[mean 48(9- l20) months). Comparing various parameters related to the recurrence, higher rate of recurrence was noted in case.I with larger tumor size, advanced T or N stages, poor histologic grading, aneuploidy and higher S-phase fraction. Conclusion: These findings suggest that flow cytometric DNA analysis may be helpful in predicting the recurrence and in selecting patients with resectable pancreatic carcinoma who will benefit from operation. (Korean 3 Gastroenterol 1995;27:237- 244)