갑상선 미세유두상암에서 혈관내피성장인자(VEGF)와 혈관내피성장인자 수용체(VEGFR-1), 저산소증 유발인자(HIF-1α)의 mRNA 발현

윤태일,정용식,고진철,함기백<SUP>1<.SUP>,소의영,Tae II Yoon,M.D.,Yong Sik Jung,M.D.,Jin Cheol Koe,M.D.,Ki Baek Ham,M.D.<SUP>1<.SUP> and Euy Young Soh,M.D. 대한갑상선-내분비외과학회 2005 The Koreran journal of Endocrine Surgery Vol.5 No.1

Purpose: Angiogeneisis is essential process for tumor growth and metastasis. Vascular endothelial growth factor (VEGF) is major regulator of angiogenesis. Recently, the incidence of papillary thyroid microcarcinoma (PTMC) increased because of development in diagnostic modality. Several recent reports have documented relationship of VEGF and papillary thyroid cancer. The aims of this study were to determine whether angiogenetic phenotype was changed or not changed and to evaluate the relationship between clinicopathologic features and VEGF, vascular endothelial growth factor receptor-1 (VEGFR-1), hypoxic induced factor-1alpha (HIF-1α) mRNA expression in PTMC. Methods: VEGF, VEGFR-1, HIF-1α mRNA expression was examined by RT-PCR in 14 patients who had undergone thyroidectomy due to PTMC. The thyroid tumor tissue and adjacent normal thyroid tissue were collected in operation and preserved at ⁣70<SUP>o</SUP>C in RNA later solution. We evaluate the expression of VEGF, VEGFR-1, HIF-1α mRNA by RT- PCR. The expression of mRNA was quantititated by densitometer and analyzed the relationship between clinicopathologic features and mRNA expression. Results: Compared to normal tissues, in PTMC we observed higher expression of HIF-1α mRNA (P=0.024) and lower expression of VEGF mRNA (P=0.002). There was no difference in expression of VEGFR-1.The patients with nodal metastasis had higher expression of the VEGF mRNA in tumor tissues than those without nodal metastasis but not significantly. The VEGF mRNA of tumor tissues in patients with thyroid capsule invasion or not were expressed similarly. The lower expression of VEGF mRNA were observed more frequently in younger patients (<40). Conclusion: The expression of VEGF mRNA was lower in tumor tissue in spite of higher expression of HIF-1α mRNA. These results suggest that the reason for good prognosis and no progression to clinical cancer in PTMC was related to the unchanged angiogenic phenotype. (Korean J Endocrine Surg 2005;5:18-23)

SVM을 이용한 만성간염 환자 예측진단을 위한 SNP정보분석

김동회(Dong Hoi Kim),함기백(Ki Baek Ham),김진(Jin Kim) 한국정보과학회 2006 한국정보과학회 학술발표논문집 Vol.33 No.1

Single Nucleotide Polymorphism(SNP)는 인간 유전자 서열의 0.1%에 해당하는 부분으로 이는 각 개인의 체질 및 각종 유전질환과 밀접한 관련이 있다고 알려져 있다. 최근 이 SNP정보의 패턴을 이용 질병의 진단 및 치료에 연관 지으려는 노력이 시도되고 있다. 그러나 아직 SNP를 이용한 효율적인 분석방법에 대한 전산학적 연구는 많지 않다. 본 논문에서는 대표적인 패턴인식기 중 하나인 Support Vector Machine(SVM)을 이용 한국인의 대표적인 유전질환으로 알려진 만성간염에 대해서 관련된 SNP에 대한 패턴 인식율 측정을 실험하였다. 실험 데이터는 간 및 소화기 질환 유전체 센터에서 얻어진 만성간염 환자와 관련 SNP정보를 사용 하였으며, 실험 결과 전체 SNP정보를 모두 가지는 환자그룹에 대한 학습인식율이 66.46%로 나타났으며, 부분그룹에서는 72.91%로 높은 인식율을 보였다. 이 결과는 SNP정보를 이용한 만성간염의 초기 진단예측에 SVM을 효율적으로 사용할 수 있음을 보인다.

간질환 환자의 갑상선 기능

이상인(Sang In Lee),조준구(Jun Koo Cho),전재윤(Chae Yoon Chon),함기백(Ki Baek Ham),정재복(Jae Bock Chung),최흥재(Heung Jai Choi) 대한내과학회 1988 대한내과학회지 Vol.34 No.3

N/A The liver has been recognized as an important site in the peripheral metabolism of thyroid homone, particularly in the mondeiodination of thyroxine to triiodothyronine. It is also the source of serum T, binding proteins. As is to be expected, disease of the liver has marked effects on serum thyroid hormone concentrations and bindings. In order to evaluate the change of thyroid hormones in various liver diseases and to elucidate the relationship between thyroid hormone status and severity of liver diseases, both thyroid hormones and liver functions were measured and analyzed in 110 patients with various liver diseases who were admitted to Yonsei University Severance Hospital during the period from April 1986 to October 1986. The results were as follows: 1) In various liver disease, acute hepatitis, fatty liver, chronic persistent hepatitis and chronic active hepatitis showed no significant difference in serum T3; T4, FT4, TSH and T3/T4 compared to normal controls. 2) The serum T3, T4 and FT4 of liver cirrhosis were signficantly lower than those of control group. but serum TSH, T3/T4 were within normal limits in liver cirrhosis. 3) The serum T3 and T4 and of hepatoma was significantly lower than those of control group, but mean serum FTTSH and T3/T4 showed no significant difference between hepatoma and control group. 4) The mean serum T3 level of seven patients with Child's class A was 100.73±25.44ng/dl, thirteen patients with class B 76.42±14.40ng/dl, and eighteen patients of class C 65.18±23.19ng/dl. There was a significant statistical difference among Child's class A, B and C. 5) There was no significant correlation between serum T3 and liver functions in Child's class A of liver cirrhosis. Serum T3 was significantly correlated with-total protein and albumin in Child's class B, with total protein, albumin and prothrombin time in Child class's C. 6) In liver cirrhosis, low serum T3 group (T3 <80 ng/dl) had a significant decrease in total protein, albumin and prolonged prothrombin time than normal serum T3 group (80 ng/dl < T3 < 220 ng/dl). 7) Serum T3 decreased significanlty in liver cirrhosis with ascites than in those without ascites. In conclusion, in liver cirrhosis and hepatoma, significant decrease in serum T3 and T4 level were observed and particularly serum T3 level may be useful in assessing the severity of hepato-cellular damage in liver cirrhosis.

Decision Tree와 SNP정보를 이용한 간경화 환자의 감수성 예측

김동회(Dong Hoi,Kim),엄상용(Saangyong Uhmn),조성원(sung_woncho),함기백(Ki Baek,Ham),김진(Jin Kim) 한국정보과학회 2006 한국정보과학회 학술발표논문집 Vol.33 No.2A

본 논문에서는 SNP데이터를 이용하여 간경화에 대한 감수성을 예측하기 위해 의사결정 트리를 이용하였다. 데이터는 간경화 환자와 정상환자 총 116명의 데이터를 사용하였으며, Feature 값으로는 간질환과 밀접한 연관성을 갖는 28개의 SNP데이터를 사용하였다. 실험방법은 각각의 SNP에 대하여 의사결정트리로 분류율을 측정한 후 가장 높은 분류율을 가지는 SNP부터 조합해 나가는 방식으로 C4.5 의사결정트리를 이용 leave-one-out cross validation으로 간경화와 정상을 구분하는 정확도를 측정하였다. 실험결과 간 질환 관련 SNP중 IL1RN-S130S, IRNGR2-Q64R, IL-10(-592), IL1B_S35S 4개의 SNP조합에서 65.52%의 정확도를 얻을 수 있었다.

SVM을 이용한 효율적인 위암관련 SNP 정보분석

김동회 ( Dong-hoi Kim ),김유섭 ( Yu-seop Kim ),천세학,천세철,함기백 ( Ki-baek Ham ),김진 ( Jin Kim ) 한국정보처리학회 2006 한국정보처리학회 학술대회논문집 Vol.13 No.1

Single Nucleotide Polymorphism(SNP)는 인간 유전자 서열의 0.1%에 해당하는 부분으로 이는 각 개인의 체질 및 각종 유전질환과 밀접한 관련이 있다고 알려져 있으며 이 SNP 정보를 이용 각종 질환의 유전적 원인규명에 대한 많은 생물학적 연구가 진행되고 있다. 그러나 아직 SNP를 이용한 효율적인 분석방법에 대한 전산학적 연구는 많지 않다. 본 논문에서는 대표적인 패턴인식기 중 하나인 Support Vector Machine(SVM)을 이용 한국인의 대표적인 유전질환으로 알려진 위암에 대한 예측율을 실험하였다. 실험 데이터는 간 및 소화기 질환 유전체 센터에서 얻어진 위 질환 환자를 대상으로 하였으며 실험 결과 예측율은 67.3%로 이는 Case Based Reasoning(CBR)방법의 55% 보다 더 좋은 예측 결과를 보였다.

自殺企圖者에 關한 硏究

奇栢錫,孫鳳基,咸 雄,金慶植,石在鎬 大韓神經精神醫學會 1980 신경정신의학 Vol.19 No.4

This paper brings up to date a study of suicide attempters coming to a major emergency room in Hang Gang Sacred Heart Hospital during a year from Oct. of 1978 to Sept. of 1979. Results of the study and the review of the 10-year-ago literature indicate that the age of the attempters becomes older in its tendency although the incidence of suicide attempts continues to be a public health problem primarily among young women. Organic phosphorous and Sodium fluoroacetates are significantly common methods used in comparison with other territory in Seoul.

급성 기종성 담낭염 2 예

이광재,김진홍,김영수,이선민,조성원,김영준,윤명호,함기백,이내희,채보원,강한걸 대한내과학회 1997 대한내과학회지 Vol.53 No.3

Emphysematous cholecystitis is an uncommon form of acute cholecystitis characterized by the presence of gas within the wall, lumen of the gall bladder or biliary ducts. Clinically it is very similar to ordinary farm. But since the risk of perforation is five times that expected from ordinary cholecystitis, early diagnosis and appropriate surgical treatment are important. We could diagnose these cases by the simple abdomen, abdominal ultrasound and abdominal CT by the presence of air in the lumen and the wall of the gall bladder. Percutaneous trans hepatic gall bladder drainage (PTGBD) for decompression was used because poor general condition of patients and later, we could successfully perform the cholecystectomy without any complication. We presented two cases of emphysematous cholecystitis with review of the relevant literature on the subject.