방풍의 항염 효과 기전

노성일 ( Sung Il Noh ),김상돈 ( Sang Don Kim ),박성철 ( Sung Cheul Park ),서병윤 ( Byung Yun Seo ),염승룡 ( Seung Ryong Yeom ),권영달 ( Young Dal Kwon ),신병철 ( Byung Cheul Shin ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.2

목적 : 방풍은 임상적으로 관절염을 포함한 다양한 염증성 질환 치료제로 사용되어 왔다. 본 연구에서는 인간 비만세포를 이용하여 세포 독성에 영향을 주지 않는 농도에서 방풍의 항염 효과 및 그 기전을 검토했다. 방법 : 인간의 HMC-1세포를 IMDM에서 페니실린, 스트렙토마이신, 모노티오글리세린를 첨가하여 배양하고 방풍추출액을 투여하였다. 그 다음 MTT, ELISA, RT-PCR, 세포내 칼슘측정, 핵단백분석을 이용하여 TNF-α, IL-6, IL-8 각각의 형성과 mRNA발현, 세포내 칼슘 수준, NF-κB 발현에 대한 방풍추출액의 반응을 측정하고 통계처리 하였다. 결과 : 방풍은 PMA와 calcium ionophore A23187로 활성화된 비만세포에서 세포내 칼슘 수준과 NF-κB, TNF-α와 IL-6의 발현을 억제 시켰고 RT-PCR을 이용한 mRNA 발현에서 TNF-α와 IL-6의 발현을 억제하였다. 결론 : 방풍은 비만세포내 칼슘 수준 및 NF-κB의 활성을 억제하고 염증성 세포 활성 물질인 TNF-α와 IL-6의 분비도 억제하여 항염 효과를 나타냄을 암시하고 있다.

고려홍삼의 수지상세포 활성화 효과

김도순(Do-Soon Kim),박정은(Jueng-Eun Park),서권일(Kwon-Il Seo),고성룡(Sung-Ryong Ko),이종원(Jong-Won Lee),도재호(Jae-Ho Do),이성태(Sung-Tae Yee) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.3

본 연구에서는 정관장 홍삼의 물(water) extract, 식용발효 주정 extract 및 홍삼 추출물로부터 분리 제조한 crude saponin을 이용하여 면역반응을 매개하는 수지상세포의 활성효과에 대하여 알아보았다. 그 결과 홍삼시료 중, crude saponin 100 ㎍/㎖을 처리하였을 때 수지상세포의 세포표면분자인 MHC class II, CD40, CD80, CD86의 발현이 증가하였으며, phagocytosis는 감소하였다. 또한 홍삼시료를 처리한 수지상세포와 allogeneic T세포를 함께 배양하였을 때, 홍삼시료의 물 extract, 식용발효주정 extract, crude saponin 모두 allogeneic T세포의 증식반응을 유도하였고, IL-2와 IFN-γ의 생산량을 증가시키는 것을 확인하였다. 또한 CD4? syngeneic T세포와 CD8? syngeneic T세포의 반응에서도 T세포의 증식반응을 높게 유도하였으며, CD4? syngeneic T세포에서 IL-2와 IFN-γ의 생산량을 증가시키고, CD8? syngeneic T세포에서는 IFN-γ 생산량을 증가시키는 것을 확인하였다. 이상의 결과로 crude saponin의 경우 수지상세포의 세포표면 공동자극분자의 발현을 유도하고 성숙을 유도함으로써 T세포의 활성을 증진시키는 것으로 생각되며, 물 extract와 식용발효주정 extract는 crude saponin과는 다른 기작으로 T세포 활성화를 유도하는 것을 알 수 있었다. 따라서 실험에 사용한 홍삼시료, 즉 물 extract, 식용발효주정 extract, crude saponin 모두 수지상세포의 활성을 유도하는 물질로써 암항원 특이적 T세포 활성화를 이용한 항암치료에 이용할 수 있는 가능성이 있다고 사료된다. Ginseng is a medicinal herb widely used in Asian countries. Dendritic cells(DCs) play a pivotal role in the initiation of T cell-mediated immune responses, making them an attractive cellular adjuvant for use in cancer vaccines. In this study, we examined the effects of Red-ginseng(water extract, edible and fermented ethyl alcohol extract, crude saponin) on the DCs phenotypic and functional maturation. Immature DCs were cultured in the presence of GM-CSF and IL-4, and the generated immature DCs were stimulated by water extract, edible and fermented ethyl alcohol extract, crude saponin and LPS, respectively, for 24hours. The expression of surface co-stimulatory molecules, including MHC(major histocompatibility complex) class II, CD40, CD80 and CD86, was increased on DCs that were stimulated with crude saponin, but antigen-uptake capacity was decreased. The antigen-presenting capacity of Red-ginseng extracts-treated DCs as analyzed by allogeneic T cells proliferation and IL-2, IFN-γ production was increased. Furthermore, CD4? and CD8? syngeneic T cell(OVA-specific) proliferation and IFN-γ production was significantly increased. However, CD4? syngeneic T cell secreted higher levels of IL-2 in responding but not CD8? syngeneic T cell. These results indicate the immunomodulatory properties of Red-ginseng extracts, which might be therapeutically useful in the control of cancers and immunodeficient diseases through the up-regulation of DCs maturation.

Cell source-dependent <i>in vivo</i> immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs

Lee, Won-Jae,Hah, Young-Sool,Ock, Sun-A.,Lee, Jae-Hoon,Jeon, Ryong-Hoon,Park, Ji-Sung,Lee, Sang-Il,Rho, Na-Young,Rho, Gyu-Jin,Lee, Sung-Lim Elsevier 2015 Experimental cell research Vol.333 No.2

<P><B>Abstract</B></P> <P>The <I>in vitro</I> differentiation and immunosuppressive capacity of mesenchymal stem cells (MSCs) derived from synovial fluid (SF-MSCs) and bone marrow extract (BM-MSCs) in an isogenic background of minipigs were comparatively analyzed in a collagen-induced arthritis (CIA) mouse model of rheumatoid arthritis (RA). The proliferation capacity and expression of pluripotent transcription factors (Oct3/4 and Sox2) were significantly (<I>P</I><0.05) higher in SF-MSCs than in BM-MSCs. The differentiation capacity of SF-MSCs into adipocytes, osteocytes and neurocytes was significantly (<I>P</I><0.05) lower than that of BM-MSCs, and the differentiation capacity of SF-MSCs into chondrocytes was significantly (<I>P</I><0.05) higher than that of BM-MSCs. Systemic injection of BM- and SF-MSCs significantly (<I>P</I><0.05) ameliorated the clinical symptoms of CIA mice, with SF-MSCs having significantly (<I>P</I><0.05) higher clinical and histopathological recovery scores than BM-MSCs. Furthermore, the immunosuppressive properties of SF-MSCs in CIA mice were associated with increased levels of the anti-inflammatory cytokine interleukin (IL)-10, and decreased levels of the pro-inflammatory cytokine IL-1β and osteoclast-related sRANKL. In conclusion, SF-MSCs exhibited eminent pluripotency and differentiation capacity into chondrocytes, addition to substantial <I>in vivo</I> immunosuppressive capacity by elevating IL-10 and reducing IL-1β levels in CIA mice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Immunosuppressive capacity of BM-, SM-, and SF-MSCs was evaluated in an RA model. </LI> <LI> Proliferation, pluripotency and chondrogenic differentiation capacity were higher in SF-MSCs. </LI> <LI> SF-MSCs exhibited improved therapeutic effects than BM-MSCs. </LI> <LI> SF-MSCs may have applications as immunosuppressive therapy in autoimmune diseases. </LI> </UL> </P>

웨이브릿변환을 이용한 서브밴드 구조 FIR 적응필터

박순규,최일홍,김자룡 진주여자전문대학 1996 論文集 Vol.19 No.-

A adaptive filter structure based on the subband decomposition of FIR filter using the wavelet transform is designed and tested. There is no sampling rate alternation in this structure and therefore no aliasing problem occurs. The adaptive noise reduction characteristics using the Harr wavelet transform and DFT are presented and compared. The structure using the Harr wavelet transform is computationally efficient compared to the DFT(FFT) method.

자동차 조립작업자의 사회ㆍ심리적 스트레스와 근골격계 증상과의 연관성

김일룡,김재영,박종태,최재욱,김해준,염용태 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.3

목적 : 이 연구는 자동차 조립라인 근로자들의 사회·심리적 스트레스와 작업관련 근골격계 자각증상간의 연관성을 확인하는 데에 있다. 방법 : 경인지역 소재 자동차 회사 두곳의 조립라인에 근무하는 근로자 636명을 대상으로 하여, 근골격계 자각증상에 대해서는 목과 어깨, 팔과 팔꿈치,. 손목과 손, 허리부위에서의 통증, 쑤심, 뻣뻣함, 화끈거림, 무감각 또는 저림 등을 묻는 표준화된 설문지로 질문하였고, 스트레스 정도는 4개 분야, 45개 항목으로 이루어진 Psychosocial Well-being Index 설문지를 배포후 3점 척도를 적용하였다. 이들간에는 카이자승법, ANOVA rm리고 로짓회귀분석을 사용하여 통계적으로 유의한 연관성을 알고자 하였다. 결과 : 1. 수면시간을 제외하고 연령, 근속기간, 결혼여부, 학력수준, 흡연력, 음주력에 따른 상지근골격계 증상의 빈도 차이는 없었다. 2. 30세 미만의 작업자군에서 사회 심리적 스트레스 점수(p-value: 0.0461)와 스트레스 Factor 3 (p-value: 0.0368), Factor 4 (p-value: 0.0053)의 점수가 높았다. 3. 11∼15년의 근속기간을 가진 작업자의 경우 Factor 1을 제외한 PWI(p-value: 0.0001), Factor 2(p-value:0.0001), Factor 3(p-value: 0.0004), Factor 4(p-value: 0.0001)에서 기타의 근속기간을 갖는 동료에 비해 높은 스트레스 점수를 보였다. 4. 미혼자는 Factor 4에서 기혼자보다 높은 스트레스 점수를 보였다(p-value: 0.0068). 5. 수면시간이 6시간 이하인 작업자군은 6시간을 초과하는 작업자보다 PWI(p-value: 0.0087), Factor 2(p-value: 0.0194), Factor 3(p-value: 0.0048), Factor 4(p-value: 0.0314) 등에서 높은 스트레스 점수를 보였다. 6. 스트레스 점수는 정규분포를 이루었으며 정상군과 스트레스위험군간에 증상의 빈도 차이는 없었다. 7. 근골격계 증상이 있는 작업자군은 무증상군과 비교하여 기타 스트레스 점수는 두 군간에 유의한 차이가 없었으나, Factor 2에서 통계적으로 유의하게 높은 스트레스 점수를 보였고(p-value: 0.028), 단변량(OR: 1.02) 및 다변량(OR: 1.04) 로짓 회귀분석에서도 Factor 2가 양의 회귀계수(0.0226; 0.0352)를 보여 증상에 영향을 주는 것으로 나타났다. 결론 : 근골격계 자각 증상이 있는 조립작업자군은 증상이 없는 군에 비해 우울증 항목에서 높은 스트레스 점수를 보였으나 기타 사회적 역할 수행 및 자기신뢰도, 수면장애 및 불안, 일반건강 및 생명력등의 사회심리적 스트레스 항목에서는 두 군간에 유의한 차이를 보이지 않았다. 로짓회귀분석예서도 우울증이 근골격계 증상에 영향을 주는 것으로 나타나서 비록 제한적이지만 우울증에 해당하는 항목인 Factor 2와 증상간의 연관성을 일부 확인할 수 있었다. 그러나 근골격계 증상발현에는 스트레스 이외에도 인간공학적 혹은 알려지지 않은 요인들 중요하게 작용하므로 이들을 통제한 후의 추가적인 조사를 해야 할 것으로 생각된다. Objectives : To determine the relationship between stress Factors and work-related muscul-loskeletal symptoms of assembly line workers in the automobile industry. Methods : A cross-sectional study was conducted in two Factories of automobile manufacturing companies where inappropriate posture and repetitive motions were required. The total number of subjects was 636, and consisted entirely of men. The Age, length of work duration, marital status, education level, smoking status, drinking status, sleeping time and stress scores were investigated according to subgroups of general characteristics. We divided the subjects into a Reference group and a Stress risk group to compare the frequency of the variables between them. The stress scores of the Positive symptom group were compared with the Symptom free group by the t-test. To measure the stress level, the PWI(Psychosocial Well being Index) which consists of 4 Factors, totaling 45 items, was used. A standardized self-reported questionnaire was used to assess the symptoms in workers. The criteria for positive symptoms were based on Operational Definition of Work-related Musculoskeletal Disorders of the NIOSH. Results : 1) The subgroups of Age, Work duration, Marital status, Education level, Smoking status, Drinking status and Sleeping time revealed no differences in frequencies in musculoskeletal symptoms. 2) Higher PWI(p<0.05), Factor 3(p>0.05) and Factor 4(p<0.01) scores were observed in younger workers compared with older workers. 3) The workers who had work durations betwen 11-15 years showed higher PWI(p<0.01), Factor 2(p<0.01), Factor 3(p<0.01), and Factor 4(p<0.01), compared with other work duration subgroups. 4) Singles had higher Factor 4 scores(p<0.01). 5) The sleeping time of less than 6 hours a day expressed a higher PWI(p<0.01), Factor 2(p<0.05), and Factor 3(p<0.01), Factor 4(p<0.05). 6) There was no difference in the prevalence of musculoskeletal symptoms between the Reference group and Stress risk group. 7) The subjects who had Positive musculoskeletal symptoms showed a significant difference in Factor 2 scores compared with the Symptom free groups. 8) According to a univariate logistic regression analysis, Factor 2(OR: 1.02, 95% CI:1.00-1.04, p-value: 0.0291) expressed significant but mild effects on the musculoskeletal symptoms and a multivariate logistic regression showed a statistically significant effect on musculoskeletal symptoms(OR:1.04, 95% CI: 1.01-1.07, p-value: 0.0170). Conclusion : Psychosocial stress scores were not higher in symptomatic subjects compared with those who had no symptoms. Sleeping longer showed a protective effect on symptoms but this was not statistically significant. There was no relationship between the stress scores and musculoskeletal symptoms in social performance and self-confidence, general well-being and vitality. Factor 2(Depression) was statistically significant though its effect was mild. Limited to this study, We could find partial relationship between psychosocial stress(Depression) and musculoskeletal symptoms. So it could therefore be suspected that ergonomic or other unknown factors may be more significant causes of musculoskeletal symptoms but we did not investigate these.

실관막을 이용한 중금속 추출에서 물질전달에 미치는 분배비의 영향

李龍珍,金英一,陳道源,黃德基,朴東源 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.1

The liquid-liquid extractions by use of microporous hollow fiber membrane modules are fast compared with conventional extraction equipment. Because they have a large surface area per volume. Solute diffuses out of the feed solution, through the fibers' porous wall, and into the extractant. The extractant and feed can be contacted at high speed, there are no problems with flooding, loading and channeling. Moreover, these modules should be characterized by low investment and operating costs because of the reduced hardware and the favorable hydrodynamics which minimizes concentration polarization and membrane fouling. In this paper, it was investigated the extraction selectivities for liquid-liquid extraction of heavy metal(Cd(Ⅱ)Co(II)) from dilute aqueous solution into the eatractants(EHPNA and TBP) by using the hydrophobic hollow fiber module. We also examined the effect of distribution ratio on the overall mass transfer coefficients.

疎水性 中空絲膜을 이용한 Cu(II)의 透過抽出

李龍珍,金萬壽,朴東源,金英一,黃德基 東亞大學校 大學院 1997 大學院論文集 Vol.22 No.-

Liquid-liquid extractions by use of microporous hollow fiber modules are fast compared with conventional extraction equipment because of the large surface area per volume. In these modules, the extractant and feed can be contacted at high speed andtwo flows are completely independent, so there are no problems with loading and channeling. In this paper, it was investigated the extractability for liquid-liquid extraction of Cu(Ⅱ) from dilute aqueous solution into D2EHPA(di-2-ethylhexylphosphoric acid), TBP(tri-n-butyphosphate) and TOA(tri-n-octylamine) as organic extractants by using the hydrophobic hollow fiber module. To determine the dependence of mass transfer rate on distribution of Cu(Ⅱ) in the module, we compared the overall mass transfer coefficient with distribution ratio. From these experiments, we identified the effect of distribution ratio on the overall mass transfer coefficient was, for the low H, the K? was increased with increasing H, but high value of H, the effect of H on K? could be negligible.

실관막에 의한 중금속이온의 추출 및 물질전달에 관한 연구

이용진,황덕기,김영일,박동원 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.2

In the microporous hollow fiber module, the extractant and feed can be contacted at high speed and two flows are completely independent, so there are no problems with loading and channeling. In this paper, it was investigated the extraction selectivities for liquid-liquid extraction of heavy metal ions from dilute aqueous solution into TOA (tri-n-octylamine) as organic extractants by using the hydrophobic hollow fiber module. From the experiments for the extraction of multicomponent system, it was known that selective separation is possible by adjusting the primary factor that have a great influence on distribution ratio, and that prediction of selective separation is also possible from the result of individual extraction systems.

연장 가골에 골 이식 대체물 투여가 골 경화에 미치는 영향 : 가토 경골에서의 방사선 및 골 밀도의 변화 Changes of Radiography & Bone Mineral Density in the Tibia of Rabbits

오창욱,김풍택,박병철,송해룡,박일형,백준호,박형진 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

목적 : 골 연장술 후 생기는 연장 가골에 황화 칼슘과 이종 이식골을 투여하여 골 경화 속도에 대한 영향을 알아보기 위한 실험이었다. 연구대상 및 방법 : 성장중인 뉴질랜드 화이트 토끼(2.0-2.5㎏)의 경골 간부에 골막을 보호하고, 절골술을 시행하고, 5일간의 휴지기후 외고정 장치를 이용하여 1주일 동안 7㎜가 연장될 때까지 하루 1㎜씩 2회의 리듬을 가지고 골 연장을 실시하였다. 제1 실험 군 7마리에는 연장이 끝난 직후 황화 칼슘(Osteoset^(ⓡ), Wright medical USA) 1정, 제 2 실험 군 7마리에는 이종 이식골의 일종인 Lubboc 5㎟을 각각 연장된 가골 부에 주입하고, 단순 연장한 대조군 7마리와 함께, 방사선 및 골 밀도비를 측정하고 추시하여, 골 경화의 진행속도를 비교하였다. 방사선 촬영은 전후면 및 측면사진을 1주 간격으로 촬영하였고, 골밀도 측정은 DEXA(dual energy X-ray absorptionmetry, Lunar^(ⓡ)) 장치를 2주일 간격으로 시행하였다. 결과 : 방사선 소견상, 제 1실험군의 연장가골의 완전 경화시간은 평균 14주, 제 2실험군은 154 주로 각각 대조군의 16.9주보다 단축되었으며, 골 밀도 비에서도, 두 실험군에서 최고치가 대조군의 값보다 높고, 최고치까지의 경과기간도 빨랐다. 결론 : 본 연구에서 연장 가골에 황화칼슘 및 이종 이식골의 투여가 골 경화의 기간을 단축시킬 수 있고, 외고정 장치의 빠른 제거에 도움이 될 수 있음을 알았다. Purpose : This study was designed to know the effect of calcium-sulfate and xenograft on the distracted callus after lengthening. Materials & Methods : We had operation of subperiosteal osteotomy and external fixation on the tibial diaphysis of young New Zealand White rabbits(2.0-2.5㎏); after 5 days of latency period, 7㎜(1㎜/day, 2times/day) of tibial lengthening was reached in a week. At 1 week after lengthening, the 1st experimental group of 7 rabbits received a pellet of calcium sulfate(Osteoset, Wright medical, USA) in the distraction gap, and the 2nd experimental group of 7 rabbits received 5㎜ 2 of xenogrfat(Lubboc) in the distraction gap. But, the control group of 7 rabbits did not receive any of above materials. We compared three groups with the changes of radiographic findings at every week and bone mineral ratio(DEXA) at every two weeks. Results : The time to complete consolidation of distraction callus of both experimental group(calcium sulfate;14 weeks, xenograft; 15.4 weeks) was shorter than that of control group(16.9 weeks) in radiographic findings. Maximum value of bone mineral ratio of distraction callus was higher and the time to reach the highest value was also shortened in the both experimental group compared to control group. Conclusion : By use of bone substitutes as like calcium sulfate or xenograft in the distraction callus with external fixator, it may be possible to shorten the consolidation period and the fixator-wearing period.

중공사막 모듈에 의한 Na의 분리에 관한 연구

정철수,김영일,황덕기,이용진,박동원 동아대학교 공과대학부설 생산기술연구소 1998 生産技術硏究所硏究論文集 Vol.3 No.1

By use of hydrophobic hollow fiber membrane module, this study examines the value of Kw(overall mass transfer coefficient) on Na^+, and used Di-2-ethylhexylphosphonic acid mono-2-ethylhexyl ester(EHPNA), Dodecyl-1,1,3,3,5,5-hexa-methyl hexylamine(LA-2), as extractant. We also shown the effect of additive(Cl^-, C_8H_17OH) and mixed extractant(Tri-n-octylphosphineoxide(TOPO)). LA-2 separated well by increased concentration of HCl and EHPNA decreased. LA-2 shown better separation ratio at compare EHPNA with LA-2. The effect of Kw vs. count ion(Cl^-) was shown that Kw increased by increased concentration of Cl^-, in effect of C_8H_17OH was weak.