Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

인터넷 기반의 전자공간을 활용한 과학기술혁신 모델

황보열,김소용 동양대학교 1999 동양대학교 논문집 Vol.5 No.1

인터넷 사용자의 폭발적인 증가와 함께 이를 기반으로 한 전자공간은 새로운 혁신을 지향하고 있다. 본 연구에서는 과학기술 혁신을 위한 전자공간 활용에 초점을 두고 국가 혁신의 새로운 모델을 도출하는 것을 목표로 하였다. 과거에는 혁신을 위한 과학기술 정보의 유통모델은 선형적인 특징을 갖고 있지만 새로운 모델은 한 곳으로 모이는 수렴(Convergence) 모델로 요약될 수 있다. 즉 새로운 과학기술 혁신 모델에서는 과학기술 정보의 공급자와 수요자가 함께 전자공간에 모여 공급과 수요가 동시에 발생하는 것이 특징이다. 결국 공급자인 동시에 수요자이고 수요자인 동시에 공급자이다. 본 연구에서는 새로운 모델을 구체화시키기 위해 정보기술을 적용한 과학기술 혁신을 위한 수요공급 아키텍쳐를 제시함과 동시에 전자공간 활용을 위한 주요 과제를 도출하였다.

韓國企業倫理에 關한 硏究

皇甫太 단국대학교 경영대학원 1981 經院論叢(壇國大學校) Vol.1 No.-

펄스정전응력법에 의한 XLPE에서 DC 과전에 의한 공간전하의 교류전압에서의 거동관찰

황보승 호남대학교 2000 호남대학교 학술논문집 Vol.21 No.2

In this paper, in order to evaluate quantitavely the formation mechanism of space charge by DC application and the behavior of pre-formed space charge at AC volatge in XLPE, We have modified the PEA method to measure simultaneously discharging (or leakage) current and space charge distribution under DC and AC. By using this system, we have analyzed the effects of pre-formed space charge accumulated by DC stress on AC in XLPE (560 ㎛) when there is no air gap between electrode and XLPE. Form the experimental, it was found that, DC conduction current decrease because of the effects of potential barrier at interface and most of pre-formed space charges still remained when AC voltage was applied up to 8kV. Thus the field distortion and localization due to pre-formed space charge is very important under AC voltage and may cause a critical damage on the AC insulating characteristics of insulator, especially in AC long-term service.

백서의 정맥혈전 모델에서 장기간의 혈전노출이 정맥변 변화에 미치는 영향

황종성,권우형,박영도,서보양,권굉보 대한혈관외과학회 2004 Vascular Specialist International Vol.20 No.1

佛語의 强勢後置傾向 硏究

皇甫淳 慶北大學校 1983 論文集 Vol.35 No.-

The Anglo-Saxon Chronicle의 어순연구 : Parker 및 Peterborough본을 중심으로

황보근 대구대학교 인문과학연구소 1988 人文科學硏究 Vol.6 No.-

The main purpose of this paper is to describe synchronic word-order types of the Parker and the Peterborough manuscripts of the Anglo-Saxon Chronicle and to explain diachronic word-order changes. The manuscripts were divided, according not to the syntactic characteristics of the sentences analyzed but to the compilers, into four sections: (Ⅰ) 878∼891, (Ⅱ) 925∼955, 971∼1001, (Ⅲ)1122∼1131 and (Ⅳ) 1132∼1154. The sentences in each entry year were analyzed and counted on the basis of three clause types: main, subordinate and relative. The number of clause types in the sections of the ASC is as follows: Ⅰ Ⅱ Ⅲ Ⅳ Main Clauses 113 65 329 249 Subordinate Clauses 13 7 109 81 Relative Clauses 28 7 66 28 Description of the word-order types led to the following conclusions: 1) The dominant word-order types in the main clause are SVX, SXV and X°VS. The frequency of SVX constantly increases from Section Ⅱ through Section Ⅳ. This type tends to have the topical or the semi-topical subject and to be used as the new information sentence conjoined with the preceding X°VS type. The SXV has the topical nominal or pronominal subject, and the pre-verbal X is mostly pronominal. The high frequency of X°VS in Section Ⅰ is due to its use as "the new event order" though it has the topical subject. The decrease in both SXV and X°VS is relatively constant. 2) In the subordinate and relative clauses SXV, SVX are the two dominant orders in Section Ⅰ. But from Section Ⅱ though Section Ⅳ the decrease in SXV is constant, and is coincident with the decrease in the main clause. The reason is to be explained by the hyphothesis that the order of elements in dependent clauses will be changed by analogy to the order of elements in independent clauses.

순천향의과대학 졸업생의 학부성적과 의사국가고시 성적의 관련성

황보영 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Objectives: This study analysed the correlation between grade point average (GPA) of medical school and the score of Korean Medical Licensing examination(KMLE). Methode: This study based on the result of the KMLE applicants who graduated from a college of medicine in 2003 and 2004. We also gathered data of these applicants from the college of medicine : gender, age, type of entrance, period of attendance, GPA of basic medicine, clinical medicine, clinical clerkships and final test scores. We analyed whether these variables affected the results of KMLE. Results: 184 applicants (90.6%) passed KMLE among 203. In the logistic regression test, GPAs of clinical medicine and final test scores were significant variables on the results of KMLE, after adjustment of covariates. Cut-off points of high risk group for failing KMLE were below 76.0(sensitivity 90; specificity 80; positive predictive value 32), 78.0(sensitivity 79; specificity 90; positive predictive value 46), 77.2(sensitivity 90; specificity 88; positive predictive value 43), 72.8(sensitivity 90; specificity 88; positive predictive value 44) in the GPAs of basic medicine, clinical medicine, clinical clerkships and final test scores, respectively. Conclusions: There were signficant associaton between the GPAs of clinical medicine and final test scores and the results of KMLE. High-risk groups of failing KMLE were the students whose GPA of baisc medicine were below 78.0, GPA of clinical clerkships were below 77.2 and final test score were below 72.8.

기능적 언어중재 프로그램이 언어장애 아동의 언어능력에 미치는 영향

황보명 대불대학교 2003 論文集 Vol.9 No.1

This study was aimed atclarifying the effects of a Functional Language Intervention in children with language disoders. Five subjects were selected and two speech-language pathologists engaged in the functional language intervention. In the functional language intervention, activities consisted of group time, palying riddles time, following directions time, drawing time, cooking time, word-chain game time, role-playing time, searching for objects based on directions, and recreation game time. To compare pre-treatment with post-treatment in the children with language disorders, repeated-measures ANOVA was used. The results were as follows; First, the Functional Language Intervention was effective in improving the number of communicative intentions of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions. Second, the Functional Language Intervention was effective in improving the number of semantic relations of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of semantic relations. Third, the Functional Language Intervention was effective in improving the overall language abilities of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the overall language abilities.

부산지역 산성강우의 장기적인 화학적 성상의 변화

황성욱,전보경,김찬훈,서정민,최금찬 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

It is important to know the impacts of acid rain because of soil acidification, reduced biological productivity. Many researchers were interested in regional acid problems, and former research were focued in short-term trends of acid rain. It is not easy to evaluate acid rain problems because of many parameters relating to meteorological factors, which are wind, temperature, humidity and other factors. Rainfall sampling for this study was done from September 1933 to December 2000 in Dong-A University, Busan. All Samples were collected in separately to early fraction and succeeding fraction. Also, the samples were measured for pH, and EC, cations and anions were analysed by ion Chromatography.