Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

( Hee-seok Seo ),( Ki Hyun Seong ),( Chang-deok Kim ),( Seong Jun Seo ),( Byung Cheol Park ),( Myung Hwa Kim ),( Seung-phil Hong ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.2

Background: Atopic dermatitis (AD) is a chronic disorder, with a vicious cycle of repetitive inflammation and deterioration of the epidermal barrier function. Adiponectin, an adipokine, has anti-inflammatory effects on various metabolic and inflammatory disorders. Recently, its level was found to be reduced in serum and tissue samples from AD patients. Objective: We aimed to investigate the effects of adiponectin on epidermal inflammation and barrier structures in AD skin. Methods: A three-dimensional in vitro epidermal equivalent model mimicking AD was obtained by adding an inflammatory substance cocktail to normal human epidermal equivalents (HEEs). The expression of epidermal differentiation markers, primary inflammatory mediators, and lipid biosynthetic enzymes was compared between adiponectintreated AD-HEEs, untreated control AD-HEEs, and normal HEEs. Results: Adiponectin co-treatment 1) inhibited the increase in mRNA expression of major inflammatory mediators (carbonic anhydrase II, neuron-specific NEL-like protein 2, thymic stromal lymphopoietin, interleukin-8, tumor necrosis factor-alpha, and human beta-defensin-2) from keratinocytes in AD-inflammatory HEEs, 2) enhanced the expression of lipid biosynthetic enzymes (fatty acid synthase, HMG CoA reductase, and serine-palmitoyl transferase), and 3) promoted the expression of differentiation factors, especially filaggrin. We also found that the expression of adiponectin receptor-1 and -2 decreased in the epidermis of chronic AD lesion. Conclusion: Activation of the adiponectin pathway is expected to enhance epidermal differentiation and barrier function as well as attenuate inflammatory response to AD as a therapeutic approach. (Ann Dermatol 31(2) 186∼195, 2019)

야쿠트어 폐쇄자음 연구-러시아어 차용어휘 분석-

강덕수 ( Duck Soo Kang ),김지선 ( Jee Sun Kim ),홍준희 ( Jun Hee Hong ) 한국외국어대학교 언어연구소 2007 언어와 언어학 Vol.0 No.39

Duck-soo Kang.Jee-sun Kim.Jun-hee Hong. 2007. A Study of Obstruents in Yakut through Analysis of Borrowed Words from Russian. Language and Linguistics 39, 1-16. One of the most important constraints in Yakut is connected with syllabic structure. Its syllabic structure is based on CV. So the constraint of "NoGeminates" is strictly applied. And the harmony between features is also considered important. Yakut has the strict constraint on the phonotactical relations. In Yakut a feature [constricted glottis] works more vigorously than [voiced]. This feature is combined with a feature [+back] or [+lab], making new constraints on the initial place: *#[+back, -CG], *# [+lab,+CG]. Finally, consonantal assimilation in obstruents of Yakut is dominated by 2 lows of constraints. VowelHarmony >> Ident-CV[pl] >> NCP >> *[+palatal]# >> Ident-IO; *#[+back, -cont][+back,-high] >> *#[+back,-CG], *# [+lab,+CG] >> [+cont] >> NoGem >> Ident-IO >> *[+voiced]#

스포츠 장면에서 목표지향적 정서모델의 타당성 검증

홍준희 한국스포츠심리학회 2002 한국스포츠심리학회지 Vol.13 No.3

인간의 심리를 구성하는 인지, 정서, 동기 중 과거에는 등한시해왔던 정서가 인지와 동기를 이끌 수 있고, 인간의 성취 행동을 이해하는데 목표추구 과정이 중요하다는 관점에서 Bagozzi, Baumgartner, 및 Pieters(1998)는 목표상황, 기대정서, 의지과정, 도구 행동, 목표획득, 목표-결과 정서로 구성된 목표지향적 정서 모델(golf-directde emotion model)을 제시하고, 그 타당성을 검증하였다. 그러나 아직 한국의 스포츠장면에서는 검증되지 못한 문제로 인해 본 연구의 필요성이 제기된다. 이러한 목적을 달성하기 위해 고등학교 운동 선수를 모집단으로 하였고, 12개의 개인 종목에서 245명, 3개의 단체 종목에서 210명이 표집되어, 총 455명이 본 연구 대상으로 표집되었다. 연구 절차는 시합이 있기 3∼4주전에 목표 확립, 기대 정서, 의지 과정을 측정하였으며, 시합이 끝난 1주 이내에 도구 행동, 목표 획득, 목표-결과 정서를 측정하였다. 자료처리는 상관분석과 공변량구조분석을 실시하였다. 이와 같은 연구 방법과 절차를 통해 목표지향적 정서 모델은 한국의 스포츠 장면에서 적합한 깃으로 나타났다 즉, 목표를 성공하거나 실패할 것으로 기대했을 때 발생하는 기대 정서가 선수들의 의지에 영향을 주고, 이 의지는 실제 행동에 영향을 주며, 이에 따라 목표획득이 결정된다는 것이다. 그리고 목표획득은 목표-결과 정서에 영향을 미치는 것으로 나타났다. Cognition, emotion and motivation comprise the mind of a human being, emotion neglected in the past can lead cognition and motivation, and goal-pursuing processes are of importance in having an understanding of the achievement behaviors of human beings. In this context, Bagozzi, Baumgartner, & Rieters(1998) presented a goal-directed emotion model, which consists of goal situation, volitional processes, instrumental behaviors, goal attainment and goal-outcome emotion, and verifying its validity. However, this model has yet to be verified in sport contexts. That is why this study is needed. This paper was picked up high-school students as a population, carrying out stratified random sampling with school. As a result, the subjects were worked with a total of 455 students. Data analyses were resorted to an correlation analysis per variable and covariance structure analysis to verify the goal-directed emotion model. The result was that the goal-directed emotion model turns out to be suitable to Korean sports area. When they expect to succeed or fail in attaining goals, the anticipatory emotion has an influence on the will of the athletes, which has an impact on actual behaviors, and these behaviors determined goal attainment and goal-outcome emotion.

정서와 목표지향적 행동간의 관계

홍준희 師範大學 體育硏究所 1998 서울大學校 體育硏究所論集 Vol.19 No.1

The purpose of this study was to investigate the effect of emotion on goal-directed behauor through the review of previous studies. The order of contents were as follows 1. It was suggested that emotion has relevance on the goal to which people will strive, give a priority to a specific goal among various goals people took, and facilitate preparedness and plan about the goal. 2. The study was inquired into the effect of anticipatory emotion about goal on the process of goal-directed behaviors 3. It was suggested that there was the effect of promotion-focus and prevention-focus goal on the different emotion response. 4. By investigating the effect of mood on the change of goal orientation, It was showed that goal orientation has the variableness according to mood.

김치 분리균인 Bacillus sp. JK-43이 생산하는 Cyclodextrin Glucanotransferase의 생산 및 특성

전홍기,배경미,김영희,백형석 부산대학교 유전공학연구소 2000 분자생물학 연구보 Vol.16 No.-

김치 시료로부터 자동산화되지 않으며 열 및 중성 pH에서 안정한 AA 유도체인 AA-2G를 생산할 수 있는 당전이활성을 가진 CGTase 생산균주를 분리하였고, 분리균주의 형태학적, 배양학적, 생리학적 성질 및 16s-rDNA sequences를 조사한 결과 그람 양성의 간균으로 호기성이며 내생포자를 형성하는 전형적인 중온성 Bacillus sp. JK-43으로 동정되었다. Bacillus sp. JK-43의 CGTase는 AA-2G 뿐만 아니라 AA-6G로 추정되는 물질을 함께 생산하였으며, 효소 최적생산조건은 1.0% soluble starch, 1.0% yeast extract, 1.0% Na_2CO_3, 0.1% K_2HPO_4 그리고 0.02% MgSO_4·7H_2O가 함유된 배지에서 pH 7.0, 37℃에서 26시간 동안 진탕배양하였을 때였다. 각종 당공여체에 따른 Bacillus sp. JK-43의 AA-2G 생산성을 조사한 결과 β-CD에서 가장 높은 AA-2G 생산성을 보였으며, 식혜제조폐액인 엿기름 및 밥당화액에서도 비교적 높은 AA-2G 생산성을 보였다. 또한 여러 가지 당수용체에 대한 JK-43의 CGTase의 당전이 반응을 검토한 결과 sucrose, mannitol 및 inositol에서 높은 당전이 수율인 70∼90%를 나타내었다. A bacterial strain, designated as JK-43, producing extracellular cyclodextrin hlucanotransferase (CGTase)[EC 2.4.1.19] was isolated from kimchi. The CGTase from isolated strain JK-43 showed the transglucosylation activity from soluble starch to L-ascorbic acid (AA) compared to those obtained from other strains. A main product formed by this reaction was identified as 2-O-α-glucopyranosyl L-ascorbic acid (AA-2G) by testing its susceptibility to α-glucosidase hydrolysis, the HPLC prodiles, and through the elementary analysis. The β-CD, γ-CD, potato starch and corn starch were identified to be suitable glucosyl donor for transglucosylation reaction on AA by CGTase. Acceptor specificity on AA-2G production was examined by use of AA, Iso-AA and AA-2P. Transglucosylation was observed toward AA-2P as well as AA. The microorganism isolated from kimchi was identified as a strain of Bacillus sp. JK-43 based on the maximal CGTase production was observed in a medium containing 1.0% soluble starch, 1.0% yeast extract, 1.0% Na_2CO_3, 0.1% K_2HPO_4, and 0.02% MgSO_4·7H_2O with initial pH 7.0. The strain was cultured at 37℃ for 26 hrs with reciprocal shaking.

자기베어링 모니터링용 광파이버센서 개발에 관한 연구

홍준희,한복수 한국공작기계학회 2003 한국생산제조학회지 Vol.12 No.1

In a high speed spindle system, it is very important to monitor the state of rotating rotor. Particularly in active control spindle system, the position sensor must provide feedback to the control system on the exact position of the rotor. In order to monitor the state of a high speed spindle exactly, high accuracy and wide frequency bandwidth of sensors are important. The focus in this paper is to make a fiber optic sensor for monitoring rotor of magnetic bearing to design the circuit for detecting optical signal, and to evaluation static and dynamic characteristics of fiber optic sensor.

내 · 외향성과 주의방향에 따른 운동강도지각(RPE)의 효과

홍준희 서울대학교 체육연구소 2002 서울大學校 體育硏究所論集 Vol.23 No.2

The purpose of this study is to identify the effects of personality type and attentional direction on PRE. The experiment was conducted using two levels of personality type(introvert, extrovert) and two levels of attentional direction(association, dissociation) as independent variables. The dependent variables were PRE and endurance time at the point of reaching exercise intensity of VO2max 50%. The task of the experiment was the treadmill task of Ellestard protocol. The subjects, who were at boy's middle school in Seoul, were divided into 7-introvert and 7-extrovert groups through pre-test. The subject's PRE was analyzed by a two-way measure ANOVA. The results of the analysis were as follows. First, PRE was not significantly different at .05 level according to personality type(introvert and extrovert). Second, PRE was not significantly different at .05 level according to attentional direction(association and dissociation). Third, PRE was not significantly different at .05 level according to personality type(introvert and extrovert) and attentional direction(association and dissociation). Although personality type and attentional direction affect PRE in the exercise intensity of VO2max 90%, personality type and attentional direction did not affect PRE in the exercise intensity of VO2max 50%.

경쟁상태불안의 방향에 대한 예측변인으로서 정서지능

홍준희,박해용 한국스포츠심리학회 2002 한국스포츠심리학회지 Vol.13 No.2

본 연구는 경쟁불안이 강도와 방향으로 구분될 수 있음을 가정하고, 정서지능이 경쟁상태불안의 방향을 예측하는 예언변인으로 작용할 수 있음을 증명하는데 그 목적이 있다. 남·여 고교 양궁선수 115명을 대상으로 조사하였으며 조사 도구로는 방향차원이 부가된 경쟁상태불안 검사지(CSAI-2: Martens, Burton, Vealey, 1990)와 이수정과 이훈구(1997)의 TMMS(Trait Meta-Mood Scale: Salovey, Mayer, Goldman, Turvey, 및 Palfai, (1995)을 사용하였다. 자료처리는 경쟁상태불안(인지와 신체불안)의 강도와 방향 그리고 정서지능의 하위 차원인 정서명확, 정서주의, 정서개선의 평균과 표준편차를 산출하였고 각 변인간의 상관관계분석 및 중다회귀분석을 실시하였다. 그 결과 인지와 신체불안간에는 정적 상관, 강도와 방향간에는 부적 상관을 나타냈다. 정서지능이 경쟁상태불안의 방향차원을 더 잘 예측할 수 있는 변인임이 드러났고, 구체적으로 정서지능의 정서개선 요인이 경쟁상태불안 중 신체불안의 방향차원을 예측하는 변인이 될 수 있음을 증명하고 있다. 새로운 경쟁불안 척도 개발의 필요성과 체험정서와 표현정서간의 차이 등에 대해 논의하였다. The purpose of this study was to examine that emotion intelligence could predict the direction of competitive state anxiety in context to supposed that competitive state anxiety was divided into intensity and direction level. The subjects were 115 high school archery. CSAI-2 included the direction dimension(Jones & Swain, 1992) and TMMS(Salovey, Mayer, Goldman, Turvey, & Palfai, 1995) are administered to athletes one day prior to game. Results of correlation analysis and multiple regression analysis indicate that the direction of competitive state anxiety was statistically significant on the emotion intelligence, in particular, in the level of emotion improvement of emotion intelligence. Results support the hypothesis that emotion intelligence could predict the direction than intensity of competitive state anxiety. This results are discussed in terms of the concept of anxiety, applications in the field, and direction of study in the future.

고속 주축 상태 모니터링용 광파이버 변위 센서 설계 제작 및 성능평가

홍준희,박찬규,신우철,이동주 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

This paper is focused on practical applicability of the optical fiber sensor considering the machine center which is going to use them. Optical fibers may be fluctuated because the machine center operates as column moving type. This causes distortion of the sensor output signal. To reduce this problem, we have improved the sensor structure and its bracket. And we evaluated performances of the sensor.

장중첩증을 동반한 맹장 지방종 1예

홍의실,나병규,김민옥,유일영,채지영,채희복,윤세진,이상전,송형근,박길선,정현용 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.2

대장 지방종은 주로 우측 결장에 호발하고 특히 회맹판 부위와 맹장 부위에 호발한다. 또한 50~60대에 빈도가 높고 증상이 있는 환자에서 장중첩증이 높게는 40~50%에서 보고되고 있어 성인 장중첩증의 원인으로 중요한 비중을 차지한다. 특히 성인 장중첩증 및 장폐쇄의 중요 원인인 악성 종양과 임상 양상이 유사하여 감별진단이 중요하다. 저자들은 반복적인 복통을 주소로 내원한 환자에서 맹장의 4×5㎝의 점막하 지방종이 말단 회장부위와 반복적인 장중첩중이 발생했던 1례를 부분 절제술로 치료하여 양호한 임상 경과를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Lipomas are one of the most common benign nonepithelial tumors of the colon which are often detected incidentally by radiologic investigation or on operation. These tumors are usually without specific symptoms. Colonic lipomas occur most often in the right colon, particularly in the ileocecal valve and the cecum. Most patients are asymptomatic. but tumors larger than 2 cm tend to produce a change in bowel habit. abdominal pain. rectorrhagia and complications including luminal obstruction or intestinal intussusception. Diagnosis can be made by colonoscopy abdominal CT. MRI, or barium enema. We report a case of lipoma of the cecum that originated in the submucosa and presented as a intussusception in a 50-year-old woman with a brief review of the literature.