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Tsuzuki, Yasuhiro,Hisanaga, Mio,Ashizawa, Koji,Fujihara, Noboru Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.10
The present study was undertaken to assess the influence of dimethyl-sulfoxide plus sucrose solution as a cryoprotectant on the adenosine triphosphate (ATP) content, the ultrastructure and the embryonic development of bovine oocytes matured in vitro. We measured the amount of ATP in cumulus cells enclosed oocytes (CO) or denuded oocytes (DO) equilibrated with or removed from the cryoprotectant (1.5 M DMSO + 0.25 M sucrose + 20% fetal bovine serum in physiological saline). As a result, the ATP contents in both CO and DO, equilibrated with the cryoprotectant, were significantly lower (p<0.05) than that of the each control group. However, ATP content of DO was recovered to the level of the control group ailer removal of the cryoprotectant, but failed to restore for CO. In the observation of the ultrastructure by a transmission electron microscope, all of the mitochondria in the ooplasm of CO and DO equilibrated with the cryoprotectant were swollen with peripherally located cristae following decreased electron density. However, a large proportion of these swollen mitochondria were restored to the normal shape which can be observed usually in the control group after removal from the cryoprotectant. To the contrary, the morphology of many mitochondria of the cumulus cells in CO were not recovered to that of the control group after removal of the cryoprotectant. CO with removed cryoprotectant had significantly lower embryonic development up to the blastocysts stage (p<0.05) after in vitro fertilization compared with that in the control group. These results suggest that the addition and removal of a cryoprotectant has a negative effect for the ATP content of cumulus enclosed oocytes. One of the factor(s) causing the lower embryonic development after removal of cryoprotectant, may be associated with ATP metabolism.
백도명,최정근,백남원,Hisanaga, Naomi,Sakai, Kiyoshi 한국산업위생학회 1998 한국산업보건학회지 Vol.8 No.2
A worker employed in a serpentine mine was found developed mesothelioma as the first case of Korea in 1997. Asbestos was known as a causative agent for mesothelioma. Thus, asbestos contamination in mines could be a big health threat to those workers who were unknowingly exposed. However, there was no report that any minerals found in Korea contained asbestos. This study was carried out to find the presence of any asbestiform fibers in minerals which could be obtained in Korean mines. We examined fifteen minerals from 44 mines which were suspected contaminated with asbestiform fibers. Asbestiform analysis was done with high resolution transmission electron microscope(TEM), with energy dispersive X-ray spectroscope(EDX) and X-ray diffraction(XRD) analyses. Among asbestiform fibers, chrysotile was found in chrysotile, serpentine, talc and pyrophylite specimens from 11 mines. Tremolite was found in tremolite and talc specimens from three mines. Mordenite was found in zeolite specimens from two mines. Wallastonite and sepiolite were found in wallastonite and sepiolite specimens respectively. Crocidolite, antigorite and actinolite were found from talc specimens. But no asbestiform fiber contaminants were found in doromite, vermiculite, limestone, marble, gypsum, kaolin, and clary specimens. Thus, these asbestiform fibers such as such as chrysotile, tremolite, mordenite, crocidolite, antigorite and actinolite could be the responsible agents for the health hazards such as mesothelioma and other cancers.
Brain Activity during Observation of Another Person`s Hand Action with a Tangible User Interface
( Shigekazu Higuchi ),( Kana Sueyohi ),( Kazuo Isoda ),( Ichiro Hisanaga ),( Stephanie Orlic ),( Yeon Kyu Kim ) 한국감성과학회 2014 춘계학술대회 Vol.2014 No.-
A tangible user interface is characterized by physical representation using graspable objects. The alpha frequency band rhythm that appears in central area (mu wave) is suppressed by observation of an action. Mu wave suppression has been used as an index of human mirror system, which reflects action recognition and empathy to others behavior. In the present study, we examined the effects of a tangible user interface on an observer`s brain activity. The participants were fifteen right-handed males. Three types of user interface for learning art objects (tangible interface, touch panel interface, and keyboard interface) were used. A manipulator grasps a model porcelain object as an input device and moves it to the plate with a sensor in front of the screen. A 64-channel electroencephalogram (EEG) was measured while the participants were observing a person manipulating the user interface. Significant mu wave suppression was found in the tangible interface condition and it was localized in the left central area. These results suggest that the tangible user interface with object-directed and goal-oriented hand actions can enhance activity of the mirror neuron system in the sensorimotor area in observers.
Yong Hwan Lee,Hee Kyung Chung,Kiyoshi Sakai,Naomi Hisanaga,Young Hyun Chung,Jeong Hee Han,Li Je Yu 한국독성학회 2001 Toxicological Research Vol.17 No.3
A patient, 58 years of age, with suspected 0/1 pneumoconiosis since 1993, complained of a dry cough and exertioning dyspnea for 6 months. He had worked in an asbestos company for more than 20 years from 1974. He was subsequently diagnosed with an interstitial lung disease during an annual special health check-up for asbestos workers. A chest X-ray showed an interstitial lung disease and high-resolution computed tomography (HRCT) showed a round opaque asbestosis with chronic hypersensitivity pneumonitis. A pulmonary function test indicated that the patient had a mild restrictive lung disease with FEVI 1.67 liters and 82% FEVI/FVC. The bronchoalveloar larvage fluid included many asbestos bodies, indicating previous exposure to asbestos. Transmission electron microscopy (TEM) using an energy dispersive X-ray analyzer (EDX) revealed many asbestos bodies consisting of mainly crocidolite fibers (6,071×10^6 fibers/g of dry lung). The patient had an unusually high asbestos content of 6,112×10^6 asbestos fibers/g of dry lung.
Han, Jeong Hee,Park, Jung Duck,Sakai, Kiyoshi,Hisanaga, Naomi,Chang, Hee Kyung,Lee, Yong Hwan,Kwon, Il Hoon,Choi, Byung Sun,Chung, Yong Hyun,Kim, Hyeon Yeong,Yang, Jung Sun,Cho, Myung Haing,Yu, Il Je Informa UK (TaylorFrancis) 2009 Journal of toxicology and environmental health. Pa Vol.72 No.21
<P>To evaluate the effects of environmental asbestos exposure on the inducement of lung cancer, pulmonary asbestos and non-asbestos fiber content was determined in 36 normal Korean subjects and 38 lung cancer subjects with no known occupational history of asbestos exposure. Pulmonary asbestos fiber content was measured by transmission electron microscopy (TEM) with energy-dispersive x-ray analysis after applying a low-temperature ashing procedure. Chrysotile fibers were the major fiber type found in the lungs of the Korean subjects. The asbestos fiber concentrations found in the lungs of normal males (25) and females (11) were 0.26 x 10(6) fibers/g of dry lung tissue and 0.16 x 10(6) fibers/g of dry lung tissue, respectively. The asbestos concentrations found in the lungs of cancer subjects were 0.16 x 10(6) fibers/g of dry lung tissue for 32 males and 0.44 x 10(6) fibers/g of dry lung tissue for 6 females. No statistical difference was found in pulmonary asbestos content between the normal and lung cancer subjects, whereas a statistical difference was noted between normal and lung cancer subjects with respect to lung non-asbestos content, indicating a potential role for non-asbestos fibers being associated with lung cancer.</P>
Case Report of Asbestos Exposure-Related Lung Carcinoma
Hee Kyung Chang,Yong Hwan Lee,Kiyoshi Sakai,Naomi Hisanaga,Yong Hyun Chung,Jeong Hee Han,Il Je Yu 한국독성학회 2002 Toxicological Research Vol.18 No.1
A 61 year-old female patient was hospitalized for lung cancer. Her occupational history indicated that she had worked for an asbestos company for 9 years from 1976. The histopathology of the lung revealed malignant bronchioalveolar adenocarcima (stage Ⅲ) in the lower-left lobe, and a lung sample was found to contain an unusually high level of asbestos, 218.9×10^6 asbestos fibers/g of dry lung tissue. The majority of asbestos fibers found was chrysotile, yet no asbestos body was detected. When compared with Korean male (0.3×10^6 fibers/g of dry lung tissue) and female subjects (0.15×10^6 fibers/g of dry lung tissue) with no known history of occupational asbestos exposure, the apparent cause of the lung cancer in the current patient was occupational exposure to asbestos.
포항지역 부검 폐조직에서 석면과 비석면 섬유농도에 관한 연구
임현술,김동훈,사카이 기요시,히사나가 나오미,김지용,Lim, Hyun-Sul,Kim, Dong-Hoon,Sakai, Kiyoshi,Hisanaga, Naomi,Kim, Ji-Yong 대한예방의학회 2000 Journal of Preventive Medicine and Public Health Vol.33 No.4
Objectives : To obtain reference values for the pulmonary asbestos and non-asbestos fiber contents of residents in Korea and to compare them with similar results from Japan. Methods : The autopsied lung specimens from 22 deceased people (20 males and 2 females) in Pohang, without any known occupational history of asbestos exposure, were analyzed for incidence of asbestos and non-asbestos fibers by transmission electron microscopy with energy dispersive X-ray analysis after using low temperature ashing procedures. Results : Chrysotite fiber (46.2%) was the major fiber type found in the lungs of the subjects. The asbestos fiber concentrations found in males and females were $0.09\times10^6$ fiberss(g of dry lungs) and $0.30\times10^6$ fibers/(g of dry lungs), respectively, showing a geometric mean concentration $0.09\times10^6$ fibers/(g of dry lung tissue), due to the predominance of males in the sample. The non-asbestos fiber contents in males and females were $4.61\times10^6$ fibers/(g of dry lungs) and $17.79\times10^6$ fibers/(g of dry lungs), respectively, with a geometric mean concentration $5.21\times10^6$ fibers/(g of dry lung tissue). Conclusions : Residents in Pohang had significantly lower levels of both asbestos and non-asbestos fibers than urban residents in Korea. Furthermore, Koreans had significantly lower levels of both asbestos and non-asbestos fibers than Japanese.