야생 등줄쥐 (Apodemus agrarius coreae) 간뇌의 Dopamine성 신경세포

정영길,이남섭,민성기,김무강 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.6

개조개, 백합, 피조개 및 꼬막 발의 조직학적 구조 및 점액질 성상에 관한 조직화학적 연구

강미경,정길남,조운복 釜山大學校 師範大學 科學敎育硏究所 2005 科學敎育硏究報 Vol.32 No.-

개조개, 백합, 피조개 및 꼬막 발의 점액질 성상을 PAS 반응, AB pH 2.5 및 AB pH 2.5-PAS, AB pH 1.0, AF pH 1.7-AB pH 2.5 염색 등의 prelectin법으로 관찰하였다. 개조개, 백합, 피조개 및 꼬막 발은 상피, 상피하결합조직 및 근층의 3층으로 구성되었으며 모든 종에서 발의 표면은 단층섬모원주상피로 덮여 있었다. 백합의 발샘의 점액샘꽈리는 작은 구형이고 소수이며 산재성이었으나 그외 종에서는 큰 구형이고 다수이며 밀집해서 나타났다. 발샘의 도관은 개조개와 피조개가 백합과 꼬막보다 더욱 발달되어 있었으며 피조개와 꼬막은 발의 선단부에 깊이 파인 홈이 나타났으며 흠에 접한 상피하결합조직에 작은 구형의 과립이 밀집해 있었다. 상피세포의 섬모에서 개조개와 백합은 강sulfomucin을, 각각 소량, 소량 내지 중등량 함유하고 있었으며 피조개와 꼬막은 sialomucin과 중성점액질을 중등량 내지 소량 함유하고 있었다. 상피 세포의 세포질은 백합에서만 강 sulfomucin을 미량 내지 소량 함유하고 있었다. 발샘의 도관은 개조개와 백합은 강sulfomucin만을 소량 내지 중등량, 피조개와 꼬막은 소량의 중성점액질과 강 sulfomucin을 중등량 또는 중등량 내지 상당량을 함유하고 있었다. 발샘의 점액샘꽈리에서 개조개 및 백합은 강sulfomucin과 약sulfomucin이 섞여 있었으며 개조개는 중등량 내지 상당량, 백합은 소량 내지 중등량 이었고 피조개와 꼬막은 강sulfomucin만을 함유하였는데 피조개는 중등량 내지 상당량을, 꼬막은 상당량 내지 다량이었다. 발의 결합조직 과립은 피조개에서 상당량 내지 다량의 강sulfomucin을, 꼬막에서 상당량 내지 다량의 중성점액질과 강sulfomucin을 함유하였다. The histochemical properties of mucins of the foot of Saxidomus purpuratus, Meretrix lusoria, Scapharca broughtonii and Tegilltarca granosa were studied prelectin methods, i.e., PAS, AB pH 2.5, Ab pH 2.5-PAS, AB pH 1.0 and AF pH l.7-ABe pH 2.5 stainings. The foots of Saxidomus purpuratus, Meretrix lusoria, Scapharca broughtonii and Tegillarca granosa were composed of three layers, i.e., epithelium, subepithelial connective tissue and muscular layers. The epithelia were lined by simple ciliated columnar cells. The foot gland of Meretrix lusoria consist of multicellular mucous acini were small spheroid in shape and a few in number and scattered, while these acini of other species were large spheroid in shape and were numerous and crowed within connective tissue. The ducts of foot glands were more developed in Saxidomus purpuratus and Scapharca broughtonii than in other species, Particularly, Scapharca broughtonii and Tegillarca granosa showed a deep groove in apical portion of the foot and small spherical granules were found in the subepithielial connective tissue in this portion. The cilia of epithelial cells of the foot gland in Saxidomus purpuratus and Meretrix lusoria were contained only small amounts or small to moderate amounts strong sulfomucins, respectively, while these alia in Scapharca broughtonii and Tegillarca granosa were contained moderate amounts or small to moderate amounts of sialomucins and neutral mucins, respectively. The epihtelial cytoplasm of Meretrix lusoria contained only minimal to small amounts of strong sulfomucins. The ducts of the foot glands in Saxidomus purpuratus and Meretrix lusoria were contained only small to moderate amounts of strong sulfomocins, while these ducts of other spicies were contained small amounts of neutral mucins and moderate or moderate to considerable amounts of strong sulfomucins. The mucous acini of these glands in Saxidomus purpuratus and Meretrix lusoria were contained moderate to considerable and small to moderate amounts of strong and weak sulfomucins, respectively. however, these acini were contained moderate to considerable amounts of strong sulfomucins in Scapharca broughtonii and considerable to large amounts of strong sulfomucins in Tegillarca granosa. The granules within connective tissue of the foot in Scapharca broughtonii were contained considerable to large amounts of strong sulfomucins, but these anules in Tegillarca ganosa were contained considerable to large amounts of neutral mucins and strong sulfomucins.

전어,볼락,말쥐치 혀의 조직학적 구조 및 Glycoconjugates에 대한 Prelectin 조직화학

정길남,강미경,조운복 부산대학교 과학교육연구소 2006 교사교육연구 Vol.45 No.-

경골어류인 전어, 볼락 및 말쥐치 혀의 구조는 H-E 염색, PAS 염색 및 AF pH 1.7-AB pH 2.5 염색으로, 혀 점액질 성상은 AB pH 2.5, AB pH 1.0, AB pH 0.4, PAS, AB pH 2.5-PAS, AB pH 1.0-PAS 및 AF pH 1.7-AB pH 2.5 염색으로 검색하였다. 1. 전어, 볼락 및 말쥐치 혀의 상피는 중층편평상피로 구성되며 점액세포들이 전어에서 혀 등쪽상피세포사이에, 볼락과 말쥐치에서 등쪽면과 하면 상피세포사이에 분포하며 모든 어종에서 등쪽면에 더 많이 분포하고 점액세포 수는 볼락에서 제일 많고 그 다음이 말쥐치, 전어였다. 2. 혀 결합조직은 전어는 섬유연골판, 탄성연골판 및 초자연골판으로, 볼락은 섬유연골판, 큰 상피양소체, 백색지방조직 및 초자연골판으로, 말쥐치는 섬유결합조직판으로 구성되어 있었다. 3. 혀 점액세포는 말쥐치는 neutral GCs만을 함유하나 전어와 볼락은 acid GCs와 neutral GCs가 다같이 나타나며 전어는 acid GCs의 양이 많았다. 전어는 nonsulfated GCs 양이 sulfated GCs 양보다 훨씬 더 많았으나 볼락은 sulfated GCs 양이 nonsulfated GCs 양 보다 더 많았다. 말쥐치는 neutral GCs만을 분비하는 점액세포 1종이, 전어는 nonsulfated GCs를 분비하는 점액세포, nonsulfated GCs와 sulfated GCs를 분비하는 점액세포 2종이, 볼락은 sulfated GCs를 분비하는 점액세포, sulfated GCs와 nonsulfated GCs를 분비하는 점액세포 및 nonsulfated GCs를 분비하는 점액세포 3종이 관찰되었다. 4. 혀 결합조직 GCs의 조직화학적 성상은 어종과 부위에 따라 차이가 있어 섬유연골판에서 전어 및 볼락은 sulfated GCs와 nonsulfated GCs가 관찰되었으며 전어는 sulfated GCs 양이 더 많았고 볼락은 nonsulfated GCs 양이 더 많았으나 말쥐치는 nonsulfated GCs만을 함유하고 있었다. 전어의 탄성연골판은 neutral GCs만을 함유하고 있었다. 초자연골판에서 전어는 sulfated GCs와 nonsulfated GCs의 혼합성이나 볼락은 sulfated GCs만을 함유하고 있었다. H-E, PAS and AF pH 1.7-AB pH 2.5 staining techniques were applied to study structure of tongues in teleosts, i.e., Clupanodon punctatus, Sebastes inermis and Cantherines modestus. For distribution of lingual mucosubstances AB pH 2.5, AB pH 1.0, AB pH 0.4, PAS, AB pH 2.5-PAS, AB pH 1.0-PAS and AF pH 1.7-AB pH 2.5 stains were performed. 1. The linings of tongues in Clupanodon punctatus, Sebastes inermis and Cantherines modestus were mucous membranes consisting of stratified squamous epithelium with mucous cells. Mucous cells existed in the dorsal surface of lingual mucosa in Clupanodon punctatus, in the dorsal and inferior surface in Sebastes inermis and Cantherines modestus. Mucous cells occurred predominantly in the dorsal surface in the all kinds of fishes studied. Sebastes inermis had the highest number of mucous cells, followed by Cantherines modestus, Clupanodon punctatus. 2. The following lingual cartilages consisted: in Clupanodon punctatus, fibrous cartilage, elastic cartilage and hyaline cartilage; in Sebastes inermis, fibrous cartilage, large epitheloid body, white fat tissue and hyaline cartilage; in Cantherines modestus, fibrous connective tissue. 3. Lingual mucous cells in Cantherines modestus contained only neutral GCs, while it contained both acid and neutral GCs in Clupanodon punctatus and Sebastes inermis. Clupanodon punctatus had much more acid GCs which consisted abundant nonsulfated than sulfated GCs. The following lingual mucous cell consisted: one kind of mucous cells secreting only neutral GCs in Cantherines modestus; two kinds of mucous cells secreting nonsulfated GCs and both nonsulfated and sulfated GCs in Clupanodon punctatus; three kinds of mucous cells secreting sulfated GCs, both sulfated and nonsulfated GCs, and nonsulfated GCs in Sebastes inermis. 4. Histochemical properties of GCs in lingual tissue have differences in species and regions. Fibrous cartilage in Clupanodon punctatus and Sebastes inermis had sulfated and nonsulfated GCs. It had only nonsulfated GCs in Cantherines modestus. Clupanodon punctatus had more sulfated than nonsulfated GCs, while Sebastes inermis had more nonsulfated GCs. Elastic cartilage in Clupanodon punctatus contained only neutral GCs. Hyaline cartilage had a mixtures of sulfated and nonsulfated GCs in Clupanodon punctatus, while it had only sulfated GCs in Sebastes inermis.

총담관 결찰후 집토끼 결장점막내 배상세포의 미세구조적 변화

고정식,강권철,양남길,안의태,박경호 순천향대학교 1991 논문집 Vol.14 No.2

담관이 폐쇄되었을 때 결장점막내 배상세포의 미세구조적 변화를 알아보기 위하여 체중 2㎏ 내외의 집토끼 총담관을 이중으로 결찰한 후 1일, 3일, 5일,7일 및 14일이 경과한 후, 결장원위부의 조직을 절취하였다. 절취된 조직은 전자현미경 관찰을 위하여 통산적 방법으로 고정, 탈수 및 포매하여 JEM 100CX Ⅱ전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 결장점막상피를 구성하는 세포중 점액을 분비하는 세포로는 장은와원주세포와 배상세포가 있다. 2. 수술후 3일군의 배상세포의 경우 점액과립중 전자밀도가 낮은 것들은 융합되어 있는 경우가 많았고, 과립형질내세망이 다소 확장되어 있었다. 3. 수술후 5일군에서는 미성숙 배상세포가 많이 관찰되었다. 4. 수술후 7일, 14일군의 배상세포는 점액과립의 생성과 분비양상이 정상세포의 것과 같아 보였다. 따라서 총담관이 결찰되면 초기에는 배상세포의 점액방출 및 생성이 억제되나 시일이 경과되면 배상세포의 분화가 활성화되어 점액의 부족분을 보완해 주어 정상상태로 회복되는 것 같다. This experiment was performed to study the morphological changes of the goblet cells in the rabbit colonic mucosa after common bile duct ligation. Healthy adult rabbits weighing about 2㎏ body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed under ether anesthesia. Experimental animals were sacrificed on the 1st, 3rd, 5th, 7th and 14th day after the operation. Mucosal specimens from the distal colon were fixed in 2.5% glutaraldelyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LHB-V ultratome, stained with uranyl acetate-lead citrate, and observed with JEM 100 CX Ⅱ electron microscope. Observed results were as follow: 1. There were two types of mucous secreting cells in the rabbit distal colon; crypt columnar cell and goblet cell. 2. In the 3rd day after bile duct ligation, there found electron lucent mucous granules in the goblet cell which were occasionally fused together, and moderately distended granular endoplasmic reticulum. 3. In the 5th day after operation, the rabbit colonic goblet cells are frequently seen immature type. 4. In the late stage of the experiments, the rabbit colonic goblet cells showed general findings of the mucous granules, and other cell organells were similar to normal ones. Considering the above findings, common bile duct ligation probably resulted the hypoformation and hyposecretion of mucous granules of goblet cells in the early stage, and may facilitate the differentiation of goblet cells in the late stage.

개조개,백합,피조개 및 꼬막 발의 복합당질에 대한 Lectin 결합양상, Alkaline 및 Acid Phosphatase 분포에 관한 조직화학적 연구

조운복,강미경,정길남 부산대학교 과학교육연구소 2005 교사교육연구 Vol.44 No.-

Lectin binding patterns of glycoconjugates of the foot of Saxidomus purpuratus, Meretrix lusoria, Scapharca broughtonii and Tegillarca granosa were studied lectin methods, i.e., 9 kinds of biotinylated lectins (DBA, SBA, PNA, BSL-1, RCA-1, sWGA, UEA-1, LCA and ConA). The activities of alkaline and acid phosphatases of the foot in these species were studied with modified Gomori's methods. Lectin binding patterns of glycoconjugates was different according to shellfish species, and protions of the foots. Lectin binding patterns of the epithelial cilia of the foots showed 9 biotinylated lectins binding in Saxidomus purpuratus, 8 biotinylated lectins binding except SBA in Meretrix lusoria, DBA, PNA, RCA-1 and LCA binding in Scapharca broughtonii and lectins of UEA-1, LCA, and ConA binding in Tegillarca granosa. Especially, these cilia showed moderate or moderate to intense with SBA and BSL-1 binding in Saxidomus purpuratus, and DBA binding in Scapharca broughtonii and Meretrix lusoria, respectively, but moderate with LCA binding in Tegillarca granosa. The cytoplasm of the foot epithelial cells appeared 6 lectins binding except SBA, UEA-1 and RCA-1 in Tegillarca granosa, while 9 lectins binding in other species. Especially, these cytoplasm showed a moderate with ConA binding in Tegillarca granosa, sWGA binding in Meretrix lusoria, LCA, sWGA, RCA-1 and PNA binding in Scapharca broughtonii, but moderate to intense with LCA binding in Saxidomus purpuratus, and DBA and LCA binding in Meretrix lusoria. Lectin binding patterns of glycoconjugates in the duct and mucous acini of the foot glands occurred 5 lectins affinity except PNA, sWGA, UEA-1, LCA and ConA in Saxidomus purpuratus, 8 lectins affinity except RCA-1 in Tegillarca granosa, and 9 lectins affinity in other speices. These mucous acini occurred intense with sWGA binding, moderate with BSL-1 and SBA in Scapharca broughtonii, and moderate to intense with sWGA in Saxidomus purpuratus, with BLS-1, RCA and sWGA in Meretrix lusoria, with SBA and BSL-1 in Tegillarca granosa, but moderate with DBA, ConA and sWGA in Tegillarca granosa. In the intraepithelial duct of these glands, sWGA binding was weak to moderate in Saxidomus purpuratus, and moderate in other species. These ducts showed a moderate affinity for SBA, PNA and RCA-1 in Scapharca broughtonii, ConA in Tegillarca granosa, and UEA-1 in Meretrix lusoria. On the other hand, the ducts of these glands within connective tissue showed a moderate to intense affinity for RCA-1 in Meretrix lusoria, a moderate affinity for SBA and sWGA in Scapharca broughtonii, and DBA, SBA, sWGA and ConA in Tegillarca granosa. In the granules of connective tissue, a weak to moderate affinity for only sWGA was found in Scapharca broughtonii. But in Tegillarca granosa, DBA, SBA, BSL-1, LCA and ConA binding were detected and DBA, SBA and BSL-1 was moderate to intense. Activities of alkaline and acid phosphatases of the epithelial cilia occurred presented faint in Meretrix lusoria, weak in Scapharca broughtonii and Tegillarca granosa. In the cytoplasms of epithelil cells, these activities were trace to weak in Saxidomus purpuratus and Meretrix lusoria, weak to moderate in Scapharca broughtonii and Tegillarca granosa. In the epithelial ducts of these glands, the activities were faint in Saxidomus purpuratus and Meretrix lusoria. In mucous acini and granules of connective tissue, the activities of alkaline and acid phosphatases were weak to moderate in only Scapharca broughtonii and Tegillarca granosa. 개조개, 백합, 피조개 및 꼬막 발의 복합당질 성상은 DBA, SBA, PNA, BSL-1, RCA-1, sWGA, UEA-1, LCA 및 Con A등 9종의 biotinylated lectin을 이용한 lectin 조직화학법 그리고 alkaline 및 acid phosphatase의 활성은 Gomori변법으로 관찰하였다. 복합당질에 대한 lectin의 결합 양상은 조개 종 및 부위에 따라 차이가 있었다. 상피세포의 섬모에 대한 lectin의 결합 양상은 개조개는 9종 lectin 모두, 백합은 SBA를 제외한 8종 lectin에, 피조개는 DBA, PNA, RCA-1, 및 LCA 4종 lectin에, 꼬막은 UEA-1, LCA 및 Con A 3종 lectin에 반응하였으며 개조개는 SBA 및 BSL-1에, 백합과 피조개는 DBA에 중등도 내지 강한 반응 또는 중등도 반응을, 그리고 꼬막은 LCA에 중등도 반응을 나타내었다. 상피세포의 세포질은 꼬막은 SBA, UEA-1 및 RCA-1를 제외한 6종 lectin에 그외 종은 9종 lectin 모두 반응하였으며 꼬막은 Con A에, 백합은 sWGA에, 피조개는 LCA, sWGA, RCA-1 PNA 및 DBA에 중등도 반응을, 그리고 개조개는 LCA에, 백합은 DBA에 중등도 내지 강한 반응을 나타내었다. 발샘 도관 및 점액샘꽈리 복합당질에 대한 lectin의 결합 양상을 보면 개조개는 PNA, sWGA, UEA-1, LCA, Con A 5종 lectin에, 꼬막은 RCA-1을 제외한 8종 lectin에, 그 외 종에서는 9종 lectin 모두에 반응하였다. 발샘의 점액샘꽈리는 피조개에서 sWGA에 강한 반응을, BSL-1 및 SBA에 중등도 반응을, 개조개에서는 sWGA에, 백합은 BSL-1, RCA 및 sWGA에 중등도 및 강한 반응을 나타내었으며, 꼬막은 SBA 및 BSL-1에 중등도 내지 강한 반응을, DBA, Con A 및 sWGA에 중등도 반응을 나타내었다. 도관에서 sWGA는 개조개에서 약한 반응 내지 중등도 반응을, 그 외 종에서는 중등도 반응을, SBA는 피조개에서, Con A는 꼬막에서 중등도 반응을 나타내었으며 결합조직 발샘 도관에서 백합은 RCA-1에 중등도 내지 강한 반응을, 꼬막은 SBA와 Con A에 중등도 반응을 나타내었다. 결합조직 과립은 피조개에 sWGA에만 약한 반응 내지 중등도 반응을 나타내었으나 꼬막에서는 DBA, SBA, BSL-1, LCA 및 Con A 5종 lectin에 반응 하였으며 DBA, SBA 및 BSL-1에 중등도 내지 강한 반응을 나타내었다. Alkaline 및 acid phosphatase의 활성은 상피세포의 섬모에서 개조개와 백합은 미약한 반응을, 피조개와 꼬막은 약한 반응을 나타내었으나, 세포질에서 개조개와 백합은 미약한 반응 내지 약한 반응을, 피조개와 꼬막은 약한 반응 내지 중등도 반응을 나타내었다. 상피의 발샘 도관은 개조개와 백합에서 미약한 반응을, 발샘의 도관과 점액샘꽈리 및 결합조직 과립은 피조개와 꼬막만 약한 반응 내지 중등도 반응을 나타내었다.

대전시 가로수 식재지 토양의 화학적 특성에 관한 연구

고소현,박관수,강길남,방병욱,김동일 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

This study was carried out to examine the effect of heavy traffic on chemical properties of soil in roadside trees of Daejeon city. Soil samples at 0~10cm and 10~30cm depths were collected from soil of the roadside trees, Platanus occidentalis. and Ginko biloba. Soil pH in heavy traffic regions were around 7.0 at 0~10 cm and 10~30cm soil depths because of spraying of calcium chloride for snow moving. The concentrations of Fe, Cd, Cu, Zn, and Pb in soil were higher in heavy traffic regions(Daejeon Station and Daehwa Industrial Complex) than in light traffic region(Chungnam National University). The result could be from rubbing and wear of car tire and metals when they travel.

신경망과 퍼지 알고리즘을 이용한 유출수문곡선 예측

이경훈,문병석,남길정,강일환 대한상하수도학회 2002 상하수도학회지 Vol.16 No.2

To develop the forecasting model on hydrologic rainfall-runoff process, this fuzzy theory is applied in this study, that is the mathematical theory to process ambiguity and the neural network theory, that is applied human's empirical thinking to mathematical process. As the result of applying neural network theory, the model of linear-linear, linear-nonlinear in the transfer function and the model applying fuzzy theory showed the excellent expectation that a coefficient of determination is over 0.97, percent error of maximum flow is less 10%. This result showed that neural network and fuzzy theory have excellent applicability in the flood forecasting. And this study expect that neural network and fuzzy theory can be used as the approach to settle problems being occurred in the course of rainfall-runoff modeling.

Validity of the Transverse Abdominis Thickness Change for Abdominal Draw-in Maneuver Using Biofeedback Sensor

Nam-Goo Kang(Nam-Goo Kang),Jong-Gil Seo(Jong-Gil Seo),Won-Nyeong Cho(Won-Nyeong Cho),Jin-Wook Sung(Jin-Wook Sung) 한국임상움직임치료학회 2022 한국임상움직임치료학회지 Vol.2 No.2

Background Transverse abdominis (TrA) is an important local muscle that plays an essential role in the core. Ultrasound imaging is a reliable and valid technique for analyzing TrA muscle thickness. However, this method requires skilled operators. Biofeedback devices have achieved similar clinical outcomes to ultrasound. Even so, few studies have examined the validity of this biofeedback. Objectives This study aimed to assess whether changes in waist circumference, as determined by changes in the thickness of TrA detected with a stretch sensor made with a biofeedback device, produce results similar to those obtained through ultrasound. Methods Nineteen healthy participants were recruited for this study. Ultrasound measurement of the thickness of TrA was compared with the simultaneous measurement of the stretch sensor biofeedback device. Results Acceptable validity was found between the thickness of the TrA and the stretch sensor displayed on-screen waist circumference (Pearson correlation = -0.69, p = 0.03). Conclusions The study confirmed that changes in the grape bar on the screen of the stretch sensor application could be employed as a suitable indicator for assessing the changes to the thickness of the TrA muscle.

INHIBITORY ACTION OF PROPOLIS EXTRACT ON THE GROWTH OF MICROORGANISMS

Gil Nam Kang,Jae Hoon Sa,Byoung Chol Kim,Kyung Hoon Kim,Chang Jin Lim 생화학분자생물학회 1993 생화학분자생물학회 추계학술발표논문집 Vol.- No.-

Poster Session 2 : Oral Presentation ; Molecular Medicine and Application : Structure-based 3D QSAR study of 5-Lipoxygenase inhibitors

( Gil Nam Lee ),( Nam Sook Kang ) 생화학분자생물학회 2005 62회 KSBMB Annual Meeting in 2005 Vol.- No.-