Production System Processes Control Activities In Enterprise Resources Planning

Hwa Gyoo Park,Dae Gyoo Cha,Jae Wan Cho 한국경영정보학회 2001 한국경영정보학회 학술대회논문집 Vol.2001 No.-

Effects of Immune Aggregates, Hydrocortisone and Angiotensin 2 Receptor Blocker on Interleukin-6 mRNA Expression in Human Mesangial Cells

Ihm, Chun-Gyoo,Park, Jae Kyung,Lee, Tae Won,Cha, Dae Ryong 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

서 론 : 여러 인자들이 interleukin-6(IL-6)의 발현을조절한다. 면역복합체와 앤지오텐신 II(AII)는 사구체신염의 발달에 관여할 것으로 알려져 있다. 따라서 면역응집물과 AII가 메산지움 세포의 IL-6 발현에 미치는 영향과 이에 대한 losartan(AII 수용체 제 1형 길항제)또는 hydrocortisone의 억제 여부를 알아보았다. 방 법 : 배양된 인체 메산지움 세포에 IgG 또는 IgA면역응집물(100 μg/mL) 및 AII(10^(-7) M)를 투여하였으며, 또한 면역응집물에 hydrocortisone(0.5 μg/mL) 또는 losartan(10-6 M)을 첨가하였다. 48시간 후에 메산지움 세포의 IL-6 mRNA의 발현을 reverse transcription-polymerase chain reaction 방법으로 측정하였다. 결 과 : 메산지움 세포의 IgG 또는 IgA 면역응집물및 AII에 의한 IL-6 mRNA 발현 : IgG 또는 IgA 면역응집물(100 μg/mL) 및 AII(10^(-7) M)는 메산지움 세포의 β-actin에 대한 IL-6의 mRNA 생산물비를 의미있게 증가시켰다. Hydrocortisone 또는 losartan의 IgG또는 IgA 면역응집물에 의한 IL-6 mRNA 발현의 조절 여부 : hydrocortisone(0.5 μg/mL)의 첨가는 IgA 면역응집물에 의한 IL-6 mRNA 발현 증가를 감소시켰으며 losartan(10-6 M) 투여는 IgG 면역응집물에 의한IL-6 mRNA 발현 증가를 감소시켰다. 결 론 : IgG 또는 IgA 면역응집물 및 AII는 메산지움 세포의 IL-6의 mRNA 발현을 증가시킴을 알 수 있었으며, 이로써 사구체신염에서의 메산지움 병변의 발달에 관여할 것으로 추정되었다. Purpose : Various factors regulate interleukin(IL)-6 expression in mesangial cells (MCs). Immune complexes or angiotensin II(AII) are involved in the development of glomerulonephritis(GN). We evaluated the effects of IgG and IgA aggregates or AII on IL-6 mRNA expression in human MCs and the modulation by losartan, an AT1 receptor blocker, or hydrocortisone. Methods : After 48 hours of culture in the presence of sera, IL-6 mRNA expression was analyzed by reverse transcription and polymerase chain reaction(PCR). Results : Incubation of MCs with IgA or IgG aggregates(100 μg/mL) as well as AII(10^(-7) M) enhanced the ratio of PCR products for IL-6 to β-actin on densitometric results. The addition of hydrocortisone(0.5 μg/mL) reduced the IgA aggregates-induced IL-6 mRNA expression and losartan(10-6 M) reduced IgG aggregates- induced IL-6 mRNA expression. Conclusion : These results suggest that IgG and IgA aggregates and AII may induce IL-6 expression in GN which can be partially suppressed by hydrocortisone or AT1 receptor blocker. (Korean J Nep h rol 20 02 ;2 1( 2 ):1 99 -2 04 )

고농도 포도당에서 배양한 혈관사이질 세포에서 안지오텐신 ll와 안지오텐신 전환효소 억제제가 Procollagen α₁(lV) m RNA 발현에 미치는 효과

임천규(Chun Gyoo Ihm),이소영(So Young Lee),차대룡(Dae Ryong Cha),조원용(Won Yong Cho),한상엽(Sang Yup Han),김형규(Hyoung Kyu Kim),조상경(Sang Kyoung Jo),윤종우(Jong Woo Yoon),김용섭(Yong Seup Kim),이정호(Jung Ho Lee) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.1

N/A Objective: Diverse glomerular disorders leadsing to progressive glomerulosclerosis share the common features of increased mRNA expression for extra- cellular matrix protein and growth factors. The precise role of angiotensin II in contributing to these disturbances is currently unknown. ACE inhibitors have been proved to be beneficial in protecting against glomerular injury in animal models and many of human glomerular diseases. Type IV collagen is a main component of extracellular matrix in the mesangium : its increased accumulation is a common pathologic finding in the glomerulosclerosis. There are some evidences that the beneficial effect of ACE inhibitor does not solely depend on the hemodynamic effect, but may be mediated by other effect. The purpose of this study is to evaluate the effects of high glucose, angiotensin II and angiotensin converting enzyme inhibitor on the expression of PCa₁(lV) in mesansial cells(MCs). Methods: Human mesangial cells were cultured with standard method. To investigate the effect of each drug and high glucose condition, MCs were cultured in the normal-glucose medium(100mg/dl) and high-glucose medium(450mg/dl), respectively. An- giotensin II and angiotensin converting enzyme inhibitor(captopril) were added to culture medium at final concentration of 10 M which is the physiologic dose in vivo. MCs were cultured in each condition for 3days, when the maximal effect of high glucose on MCs, and harvested for mesurement of the expression of PCa₁(IV) mRNA. To quantitate the PCa(1V) mRNA levels in each condition, semiquantitatine RT-PCR was done with co-amplification of house keeping gene. Results: PCa₁(IV) mRNA expression was significantly increased in high-glucose medium(30mM) compared to normal-glucose medium(5.5mM)(2.28±0.34 vs 0.96±0.08, p<0.05). Administration of angiotensin ll(10(-6)M) in culture media induced a further increment in the PC a >(IV) mRNA expression to 4.64±0.28(p<0.05). Angiotensin II in the normal-glucose medium increased the PCa₁(lV) mHNA expression as 2.69±0.23 control(p<0.05). Addition of angiotensin converting enzyme inhibitor(Capopril, 10(-6)M) in high- glucose culture medium significantly suppressed the PCar(IV) mRNA expression as 0.690.11(p<0.05). Conclusion: High glucose concentration in culture medium significantly increases the mRNA expression of procollagen alphal(IV) than normal glucose concentration. Angiotensin II increases the collagen mRNA expression directly and this effect was significantly prevented by ACE inhibitor. This result suggests that hyperglycemia in diabetic millieu can directly increase collagen production, and ACE inhibitor may inhibit progressive glomerulosclerosis by decreasing collagen production as well as reducing intraglomerular pressure.

Effect of IgA Aggregates on Transforming Growth Factor-β1 Production in Human Mesangial Cells and the Intraglomerular Expression of Transforming Growth Factor-β1 in Patients with IgA Nephropathy

( Sang Youb Han ),( Chun Gyoo Ihm ),( Dae Ryong Cha ),( Young Sun Kang ),( Kum Hyun Han ),( Hyoung Kyu Kim ),( Jee Young Han ) 대한내과학회 2005 The Korean Journal of Internal Medicine Vol.20 No.1

Overexpression of an AP2ERF-type Transcription Factor CRF5 Confers Pathogen Resistance to Arabidopsis Plants

( Ying Shi Liang ),( Netty Ermawati ),( Joon Yung Cha ),( Min Hee Jung ),( Mukhamad Su`udi ),( Min Gab Kim ),( Sun Hwa Ha ),( Chung Gyoo Park ),( Dae Young Son ) 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.2

The cytokinin response factor 5 (CRF5) belongs to a family of plant-specific APETALA2 (AP2)/ ethylene-responsive element binding proteins (EREBPs). The novel role of Arabidopsis CRF5, previously identified as a mediator of cytokinin signaling, has been suggested to increase pathogen resistance in this study. Endogenous CRF5 transcripts are expressed in all tissues, including the seedlings, leaf, stem, flower, silique and root, and were found to be induced at 1 h after infection with the bacterial pathogen, Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The results of a yeast one-hybrid assay revealed that an acidic region of CRF5, including the C-terminal 28 amino acids, functions as a transcriptional activator. Overexpression of CRF5 in transgenic Arabidopsis increases pathogen resistance and concomitantly activates the expression of a large number of GCC-box pathogenesis-related genes. These results indicate that CRF5 may be involved in disease resistance as a transcription activator, thus providing a mechanistic link between the plant pathogen response and cytokinin signaling.

Overexpression of an AP2/ERF-type Transcription Factor CRF5 Confers Pathogen Resistance to Arabidopsis Plants

Liang, Ying Shi,Ermawati, Netty,Cha, Joon-Yung,Jung, Min-Hee,Su'udi, Mukhamad,Kim, Min-Gab,Ha, Sun-Hwa,Park, Chung-Gyoo,Son, Dae-Young The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.2

The cytokinin response factor 5 (CRF5) belongs to a family of plant-specific APETALA2 (AP2)/ethylene-responsive element binding proteins (EREBPs). The novel role of Arabidopsis CRF5, previously identified as a mediator of cytokinin signaling, has been suggested to increase pathogen resistance in this study. Endogenous CRF5 transcripts are expressed in all tissues, including the seedlings, leaf, stem, flower, silique and root, and were found to be induced at 1 h after infection with the bacterial pathogen, Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The results of a yeast one-hybrid assay revealed that an acidic region of CRF5, including the C-terminal 28 amino acids, functions as a transcriptional activator. Overexpression of CRF5 in transgenic Arabidopsis increases pathogen resistance and concomitantly activates the expression of a large number of GCC-box pathogenesis-related genes. These results indicate that CRF5 may be involved in disease resistance as a transcription activator, thus providing a mechanistic link between the plant pathogen response and cytokinin signaling.

Serum interferon-γ and urinary monocyte chemoattractant peptide-1 are important factors in the pathogenesis of immunoglobulin A nephropathy

( Sang Youb Han ),( Kyung Hwan Jeong ),( Chun-gyoo Ihm ),( Young Sun Kang ),( Dae Ryong Cha ) 대한신장학회 2021 Kidney Research and Clinical Practice Vol.40 No.1

Background: Imbalance of T helper (Th) 1/2 cells has been shown to contribute to the development of immunoglobulin A nephropathy (IgAN). To address the inconsistent results on the role of Th1/Th2 polarization, we evaluated the levels of Th1/Th2 cytokines in various samples from patients with IgAN. Methods: Thirty-one patients with biopsy-proven IgAN (age, 34.48 ± 12.10 years) and 25 healthy controls (age, 44.84 ± 13.72 years) were enrolled. We evaluated the relationship between the levels of Th1/Th2 cytokines and the response to glucocorticoid treatment. Results: The levels of serum interferon-gamma (IFNγ) and urinary monocyte chemoattractant peptide (MCP)-1 were higher in the IgAN group than in the control group. The levels of MCP-1 in urine and secreted by peripheral blood mononuclear cells (PBMCs) were significantly different among three groups categorized based on daily proteinuria. The level of urinary MCP-1 was significantly correlated with proteinuria. The levels of urinary MCP-1, serum interleukin (IL)-4, IFNγ, and IL-2 secreted by PBMCs and intrarenal IL-1 messenger RNA (mRNA) were significantly correlated with the ratio of proteinuria at 6 months to baseline proteinuria in patients undergoing glucocorticoid treatment. MCP-1 mRNA and protein levels were significantly upregulated in mesangial cells stimulated with IFNγ among representative Th1/Th2 cytokines. Conclusion: IFNγ was shown to be a key cytokine in the pathogenic processes underlying IgAN, and its upregulation induced an increase in urinary MCP-1 production. These findings suggest that Th1 cytokines may play an important role in the development of IgAN.

Dirty Neck of Atopics

이동원(Dong Won Lee),변대규(Dae Gyoo Byun),차미경(Mi Kyung Cha),김진우(Jin Won Kim) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.1

We report a case of Dirty Neck in a 27-year-old male suffering from severe atopic dermatitis. 'Dirty Neck', a reticulate pigmentation of the anterior and anterolateral aspects of the neck, is a specific finding in some subjects with atopic dermatitis. Two groups have been indentified. One is found in older patients with severe atopic dermatitis whose pigmentary lesions develop after puberty and show little seasonal variation. The other is found in younger patients with mild eczema who have a increase in pigment during the summer. Our patient showed a dark brown colored, linear, reticulate pigmentation of the neck, especially anterolateral aspeet regarded as the former group. More comprehensive and detailed clinical study is needed to evaluate the importanee of this entity in regard to as a minor clinical feature of Korean atopic dermatitis. (Kor J Dermatol 1994; 32(1): 198-201)

Biochemistry : Overexpression of an AP2/ERF-type Transcription Factor CRF5 Confers Pathogen Resistance to Arabidopsis Plants

Ying Shi Liang,Netty Ermawati,Joon Yung Cha,Min Hee Jung,Mukhamad Su Udi,Min Gab Kim,Sun Hwa Ha,Chung Gyoo Park,Dae Young Son 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.2

배양된 메산지움세포에서 고농도 포도당, 안지오텐신 II 및 안지오텐신 전환효소 억제제가 TGFβ 유전자 발현에 미치는 효과

이소영,김형규,윤종우,차대룡,조원용,강민모,곽재영,임천규,한상엽,조상경 대한신장학회 1999 Kidney Research and Clinical Practice Vol.18 No.4

Objective:Diabetic nephropathy is an important cause of end stage renal disease in Korea and associated with major morbidity and mortality. The precise pathogenic mechanism of this disease is still controversial, but it has been considered that multiple factors are contribute to the development and progression of diabetic nephropathy. One of these factors, renin-angiotensin system has been proven to be a major mediator of this disease via activation of angiotensin II, which has multiple functions such as induction of production of extracellular matrix protein and various intraglomerular cells, tubulointerstital component and increment of intraglomerular pressure. Transforming growth factor(TGFβ) is a multifunctional cytokine with major profibrotic character, which stimulates the production of extracellular matrx(ECM) protein, inhibit the degradation of ECM and induce the interaction of mesangial cells with ECM via integrin receptors. This study was done to evaluate the role of angiotensin II and angiotensionverting enzyme inhibitor in expression of TGFβ mRNA which is a main mediator in the pathogenesis of diabetic nephropathy. Methods:Human mesangial cells(MCs) were cultured by standard culture techniqne. For this study, cells in the 5th to 7th passage were used. To make a different glucose concentration in culture medium, normal(100mg/dl) or high glucose(450mg/dl) concentrations of D-glucose were added, and cultured in 17% heat inactivated fetal bovine serum. Angiotensin II and ACE inhibitor(captopril) were administered to the culture medium at final concentration of 10-6M. After 72 hours, MCs were harvested to measure the expression of TGFβ mRNA. To measure the mRNA expression of TGFβ in each condition, semi quantitative PCR was done and all results were corrected by β-actin gene. Results:mRNA expression of TGFβ was significantly increased in the high glucose medium(30 mM) compared to normal glucose medium(5.5mM) (3.82±0.465 vs 2.27±0.13, p$lt;0.05). Administration of angiotensin II(10-6M) in high gluum induced a further increase in the TGFβ expression to 4.29±0.476(p$lt;0.05). Angiotensin II(10-6M) in normal glucose medium also showed a significant increase in TGFβ expression as 3.40±1.88(p$lt;0.05). Administration of ACE inhibitor(Captopril, 10-6M) in high glucose medium prevented the increse of TGFβ expression(1.20±0.18 vs 3.82±0.465, p$lt;0.05). Conclusion:From these findings, it suggest that angiotensin II is an important mediator in the pathogenesis of diabetic nephropathy. ACE inhibitor may have a role in the progress of this disease via direct suppression of TGF? system as well as beneficial intraglomerular hemodynamic effect.