Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway

Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27

<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>

자외선의 조사간격이 브로일러 병아리의 볏 피부중 비타민 D₃함량에 미치는 영향

조인호,장윤환,이은택,여영수,배은경,김중달 한국축산학회 1994 한국축산학회지 Vol.36 No.1

This study was conducted to determine the content, of previtamin D₃(PreD₃), lumisterol₃(L₃), vitamin D₃(VD₃) and provitamin D₃(ProD₃) in comb skski of broiler chicks exposed to medium ware ultraviolet(UVB) lights in different interval. The broiler Hubbard line day old chicks(199 = 10 control + 3 irradiation interval × 9 elapsed time × 7 replica) were fed vitamin D deficient diet for 3 weeks in a windowless subdued light room and exposed to 297 ㎚ UVB light by 0.068 mJ/㎝-(10 min) three times in 0, 12 or 24 h interval. The comb skin were taken at 0, 6, 12, 18, 24, 48, 96, 144 or 240 h after last irradiation, and epidermis and dermis were separated. The lipid in sample was extracted by 9% ethyl acetate/hexane and purified by Sep-Pak silica catridge. The stright phase HPI-C was applied to analyze the concentration of Prop; and its photoproducts. When chicks were exposed once to UVB light for 30 min without interval, the mole % of ProD₃ in comb epidermis were 100% at control and 52.65% at 0 h after irradiation, thereafter it increased gradually to 88.17% at 240 h. PreD₃ and L₃ presented the maximum mole % at 0 h. VD₃ showed the peak value at 12 h. then decreased slowly. As UVB light was utilized to irradiate the chicks for 10 thin three times in 12 h interval, the ProD₃ mole portion in epidermis at 0 h was 76.4%, the lowest value among tested. PreD₃ and 1-3 preserved the highest level at 24 and 0 h, respectively, thereafter decreased gradully. VD₃ showed a peak at 6 h after exposure. When 24 h interval system was treated, the lowest value of ProD₃ 83.52% was appeared at 0 h. PreD₃ and L3 showed the highest level at 6 and 0 h, respectively. Mole ale of VD₃ had a peak value at 6 h and thin decreased. The mole % of ProD₃ and its photoproduets in comb dermis presented similar trends of time course variation as in those in epidermis. In respecting the method of UVB irradiation the PreD₃, L, and VDT were produced more quickly and largely in no intend system as compared to the time and amount produced in 12 or 24 h interval system.

Calculations of AC magnetic losses from the experimental field profiles in various types of coated conductors under applied fields

Yoo, J,Lee, S,Jung, Y,Lee, J,Youm, D,Ha, H,Kim, H,Ko, R-K,Oh, S Institute of Physics 2008 Journal of physics. Conference series Vol.97 No.1

<P>We measured the field profiles, <I>H</I>(<I>x,H</I><SUB>a</SUB>) s, near the surface of coated conductors (CCs) by using the scanning Hall probe method. The samples were SmBCO-CC tape fabricated by co-evaporation method and YBCO-CC tape fabricated by PLD method. The applied fields, <I>H</I><SUB>a</SUB>s, were decreased from <I>H</I><SUB>peak</SUB>to -<I>H</I><SUB>peak</SUB> stepwise. From the values of <I>H</I>(<I>x,H</I><SUB>a</SUB>), we calculated the current profiles, <I>J</I>(<I>x,H</I><SUB>a</SUB>) s, by the inversion method. From the values of <I>J</I>(<I>x,H</I><SUB>a</SUB>) and the corresponding flux densities, we calculated the hysteretic energy losses per cycle, <I>Q</I><SUB>M</SUB>s, for various <I>H</I><SUB>peak</SUB>s. From the values of <I>Q</I><SUB>M</SUB>, we calculated the characteristic functions, <I>g</I>s, by using the relation, <I>g</I>= π<I>Q</I><SUB>M</SUB>/μ<SUB>0</SUB><I>I</I><SUP>2</SUP><SUB>c</SUB>. Here, <I>I</I><SUB>c</SUB> is the critical current. For the range of <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB>≤ 3, the <I>g</I>-values of SmBCO CC tape were larger than those of YBCO CC tape. However, for the range of <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB> ≥ 3, the <I>g</I>-values of SmBCO CC tape were smaller than those of YBCO CC tape. When <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB> = 3, both sample show almost same value of <I>g.</I>However we found qualitatively different <I>J–B</I> hysteretic curves for both samples. We also compared our <I>g</I>-values with other <I>g</I>-values, which were directly measured by energy loss experiments. Our <I>g</I>-values of YBCO CC tapes were basically similar to the Brandt's theoretical values of <I>g</I> in the most range of <I>I</I><SUB>peak</SUB> in our measurements.</P>

두개골의 단순전후 방사선 사진을 이용한 전두동의 분류 및 그 분포에 관한 연구

이혜경,권귀향,김기정,강신몽,문국진 순천향대학교 2002 Journal of Soonchunhyang Medical Science Vol.8 No.4

H-bridge 멀티-레벨 인버터의 파워Cell AVR에 관한 알고리즘

전재현(J.H.Jeon),윤홍민(H.M.Yun),김민극(M.K.KIM),이정표(J.P.Lee),장동제(D.J.Jang),나승호(S.H.Na),권봉현(B.H.Kwon) 전력전자학회 2005 전력전자학술대회 논문집 Vol.- No.-

본 논문은 멀티-레벨 H-bridge 인버터에서 입력전압 변동에 따른 AVR(Auto Voltage Regulation) 기능을 적용 그 타당성을 제안하였다. 기존의 범용 인버터에서 (V/F)로 구동되는 전동기 시스템에 있어서 인버터에 공급되는 입력 전압은 출력 주파수에 따라 출력 전압 비율을 일정하게 하고 기동에 필요한 전압을 더하여 출력하는 방식이다. 집중 제어 방식의 멀티-레벨 H-bridge 인버터에서는 Cell의 DC-Link 전압을 Master에서 받아들여서 각각의 Cell에 기준 전압값을 지령하게 된다. 그러므로 입력 전압 변동에 따른 DC-Link 전압의 변동이 발생하게 되면 상전압 Unbalance 가 발생하게 되어 부하가 원하는 출력 전압을 낼 수가 없게 된다. 또한 각각의 Cell을 제어하는 Master 제어기가 가지고 있는 문제점을 보완하여 각각의 Cell 제어기 스스로가 AVR을 수행하는 좀더 나은 방법을 제안하였다.

제주지역의 도입육우에 관한 연구 : 2. Angus , Charolais , Hereford 및 이들 교잡종의 발육에 관한 연구 2. A Study of Growth of Angus , Charolais Polled Hereford and their Crossbreds

이희석,김동철,김영우,김용배,김중계 한국축산학회 1984 한국축산학회지 Vol.26 No.7

Performance of introduced Angus, Charolais, Polled Hereford and their cross breds (Angus × Hereford and Charolais × Hereford) were studied. 1. Charolais (31.5 Kg) and Polled Hereford (30.2 Kg) were considerably larger at birth than Angust (25.2Kg) but at 12 months Angus (217.3 Kg) was heavier than Polled Hereford (155.9 Kg) or Charolais (184.2 Kg). In cross breds the birth weight of C × H (32.7 Kg) was heavier than A × H (30.5 Kg). However there was no significant difference in weight at 12 months between the tow cross breds. 2. Males were heavier at birth than females and the weight at 12 months was still in favour of male. 3. Heterosis in birth weight was 9.9% in A × H and 5.8% in C X H but only C × H showed heterosis in weight after 6 months. 4. Average daily weight gain in feed lot was greater in Chalorais (1.15 Kg) than Angus (1.04 Kg) or Polled Hereford (1.06 Kg). In cross breds daily weight gain was greater in A × H than C × H. The dressing percentage was higher in cross breds (53.0%) than pure breds (52.5%). 5. Effect of sex on the daily weight gain and carcase percentage was greater in Charolais male calves (1.15 Kg) than Polled Hereford (1.05 Kg) or Angus (1.04 Kg) male calves. A × H male gave the higher daily weight gain (1.17 Kg). Dressing percentage of cross breds was also higher (53.1-53.3%) than the pure breds (51.6-52.9%).

Heat transfer with fully developed slot jets impinging on confined concave and convex surfaces

Lee, D.H.,Kim, S.J.,Kim, Y.H.,Park, H.J. Pergamon Press ; Elsevier Science Ltd 2015 INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER - Vol.88 No.-

This research investigates the laminar heat transfer characteristics of a milli-scale confined slot jet impinging upon concave and convex surfaces at a uniform heat flux. The flow at the nozzle exit has a fully-developed velocity profile. The effects of Reynolds number (Re), nozzle-to-surface distance (H/B) and the geometrical shape of target surface on local heat transfer are investigated. The heat transfer measurements are conducted at Reynolds numbers ranging from of 200 to 600 and H/B from 2 to 12. The results show that the local Nusselt numbers for both surfaces increase with increasing Reynolds number. The axial distributions of local Nusselt numbers exhibit the peak at the stagnation point and monotonically decrease in the wall jet region, showing a bell-shaped for all Re's and H/B's tested. The results also show that the peak stagnation point Nusselt numbers occur at around H/B=5-7 for the convex surface. On the contrary, the stagnation point Nusselt numbers for the concave surface tend to gradually decrease as the nozzle-to-surface distance increases. The stagnation point Nusselt numbers for the concave surface are higher than those for the convex surface at all Re's and H/B's tested.

갑상선자극 호르몬 분비에 대한 Dopaminergic Control에 관한 연구

이정상,이문호,고창순,김응진,김명덕 대한핵의학회 1978 핵의학 분자영상 Vol.12 No.2

1978년 4월부터 1978년 8월사이에 서울대학교병원 내과에 내원하였던 원발성 갑상선기능저하증 환자 9명과 일반신체검사 및 갑상선기능검사상 전혀 이상이 발견되지 않은 의과대학생 6명과 수련의 1명 총 16명을 대상으로 Dopamine 수용체를 선택적으로 차단하는 Metoclopramide와 Dopamine 수용체를 선택적으로 자극하는 Bromergocryptine(CB-154)을 각각 혹은 함께 투여해서 다음과 같은 결과를 얻었다. 1) Metoclopramide(Moxolonⓡ) 10mg을 정맥으로 주사했을때 원발성 갑상선기능저하증 환자들에서는 투여후 20분에 말초혈액에서 갑상선자극호르몬이 통계학적으로 유의하게 증가되었으며, 60분에도 계속 증가되었다. 그 이후 180분까지 계속 증가된 장태를 유대했으며, 중증보다 경증에서 더욱 현저하게 증가되어 나타났다. 그러나 정상인에서는 이러한 증가가 나타나지 않았다. 2) Bromergocryptine(CB-154)을 2mg 경구투여했을때 원발성 갑상선기능저하증 환자들에서는 투여후 120분에 말초혈액에서 갑상선자극호르몬이 현저하게 감소되었고, 240분 및 360분까지 계속 감소되었으며, 중증보다 경증에서 더욱 현저하게 감소되어 나타났다. 그러나 정상인에서는 이러한 감소가 나타나지 않았다. 3) Bromergocryptine(CB-154) 2mg을 경구투여한 후 120분에 Metoclopramide(Moxolonⓡ) 10mg을 정맥 주사했을때 원발성 갑상선기능저하증 환자들에서는 Bromergocryptine을 투여한 후 120분에 말초혈액에서 갑상선자극호르몬이 감소했으며, Metoclopramid를 투여한 후에 약간 증가하는 추세였으나, Metoclopramide를 단독으로 투여했을 때와 같은 현저한 증가는 나타나지 않았으며, 정상인들에서는 Bromergocryptine 및 Metoclopramide를 투여했을때 뚜렷한 증가나 감소는 보이지 않았다. 4) 생리식염수 2ml를 정맥주사했을 때 원발성 갑상선기능저하증 환자들 및 정상인들에서 갑상선자극호르몬이 말초혈액에서 뚜렷한 증가 내지 감소는 보이지 않았다. 5) Metoclopramide와Bromergocryptine을 각각 혹은 함께 투여했을때 및 생리염수를 투여했을 때 혈중 Triiodothyronine 및 Thyroxine치는 각각의 기저치들에 비해 통계학적으로 유의한 증가나 감소를 보이지 않았다. 이상의 결과에서 갑상선자극호르몬의 분비가 Dopaminergic Control을 받는다는 사실을 알 수 있었다. 본 연구를 실시함에 있어 물심양면으로 협조해주신 동아제약에 사의를 표하는 바이다. To e1ucidate the depaminergic control of T.S.H. secretion, we analized the pattern of T.S.H secretion in seven normal controls and nine primary hypothyroid subjects, before and after single or combined administration of specific dopaminergic receptor blocker, metoclopramide, and specific depaminergic receptor stimulant, bromergocryptine (CB-154). The results obtained were as follows: 1) There was a significant rise in T.S.H. levels after intravenous injection of metocioramide(10mg) in hypothyroid subjects. But there was no significant rise in T.S.H. levels in normal controls. The T.S.H. response to metoclopramide varied considerably, being large in mild cases and small in severely hypothyroid subjects. 2) There was a significant fall in T.S.H. levels after oral administration of bromergocryptine(2mg) in hypothyroid subjects, but there was no significant fall in T.S.H. levels in normal controls. 3) There was no significant fluctuation in T.S.H. levels after combined administration of both metoclopramide and bromergocrytine. 4) There was no significant fluctuation in T.S.H. levels after intravenous injection of normal saline(2ml) in both hypothyroid and normal subjects. 5) There was no significant change in serum T3 and T4 after administration of metoclopramide and bromergocryptine respectively and serially. These data support the fact that there is a dopaminergic control in the secretioI1 of T.S.H. in the human.

Clinical outcomes of venous thromboembolism with dalteparin therapy in multiple myeloma patients

Lee, S.E.,Jeon, Y.W.,Yoon, J.H.,Cho, B.S.,Eom, K.S.,Kim, Y.J.,Kim, H.J.,Lee, S.,Cho, S.G.,Kim, D.W.,Lee, J.W.,Min, W.S.,Kim, M.,Min, C.K. Pergamon Press 2015 Thrombosis research Vol.136 No.5

This study focused on the clinical outcomes in multiple myeloma (MM) patients with venous thromboembolism (VTE) who received low-molecular-weight heparin (dalteparin) therapy. Changes in D-dimer levels before and after VTE were also evaluated. Among 549 patients treated with various chemotherapeutic agents, a total of 52 (9.47%) patients including 32 newly diagnosed with MM and 16 with relapsed/refractory MM developed VTE, 48 of whom received dalteparin. Among the 48 treated patients, 37 (77%) had proximal deep vein thrombosis (DVT), four had (8%) pulmonary embolism (PE), and seven (15%) had both DVT and PE. In 32 patients with available paired samples (at baseline and VTE occurrence), significant conversion of D-dimer levels from 2.2+/-0.4mg/L to 11.8+/-1.6mg/L (P<0.001) was observed, which decreased from 10.9+/-0.4mg/L to 1.9+/-0.6mg/L one month after initiating dalteparin therapy. A total of 44 patients received dalteparin with a median duration of 4.2months (range, 2.7-9.4), and four patients were discontinued early due to death (n=3) and major bleeding (n=1). After a median follow-up of 9.0months (range, 0.7-35.8) since the first VTE episode, five patients showed recurrence of VTE with a cumulative incidence of 17.5+/-7.9%. Major bleeding occurred in three patients. In summary, dalteparin seems to be a promising drug for the treatment of VTE in MM. In addition, the significant difference in D-dimer levels observed before occurrence of VTE and after dalteparin treatment may suggest the usefulness of D-dimer testing as a surrogate marker for VTE in MM patients.

CAN통신을 이용한 H-브릿지 멀티레벨 인버터의 PWM 동기화 및 위상전이 방법

박영민(Y.M.Park),유한승(H.S.Yoo),장성영(S.Y.Jang),이현원(H.W.Lee),이세현(S.H.Lee),서광덕(K.D.Seo) 전력전자학회 2004 전력전자학술대회 논문집 Vol.- No.-

H-브릿지 멀티레벨 인버터는 여러 개의 단상 Power Cell을 직렬로 연결함으로써 저전압 전력용 반도체를 사용하여 고전압을 얻을 수 있고, 정현파에 가까운 출력전압 파형을 얻을 수 있는 멀티레벨 인버터 토폴로지이다. 본 토폴로지는 출력전압 레벨에 비례하여 Power Cell의 수가 증가하므로, 주제어기의 연산능력에 대한 부담증가와 신호선의 많아지는 단점이 있다. 따라서 Power Cell제어를 직접적인 PWM 신호가 아닌 통신을 사용함으로써 이러한 단점을 극복할수 있으며, 신뢰성 측면이나 보수/유지 측면에서도 유리하다. 본 논문은 산업현장에서 신뢰성을 인정받아 많이 사용되고 있는 직렬통신 방식의 일종인 CAN통신 인터럽터를 이용한 H-브릿지 멀티레벨 인버터 Power Cell의 PWM 동기화 및 위상전이 방법에 관한 것이다. 제안된 방법의 주요 장점은 주제어기와 셀 제어기 사이에 직렬통신(CAN)을 사용함으로써 주제어기와 셀 제어기의 신호선의 단순화, 주제어기의 부담 감소, Power Cell의 모듈화, 셀 단위의 보호동작 용이, 확장성 향상 그리고 제어 신호 및 Power Cell의 신뢰성을 향상에 있다. 13레벨로 구성된 H-브릿지 멀티레벨 인버터 시험을 통해 제안된 방법의 타당성과 신뢰성을 입증하였다.