A cellulose synthase-like protein is required for osmotic stress tolerance in <i>Arabidopsis</i>

Zhu, Jianhua,Lee, Byeong-Ha,Dellinger, Mike,Cui, Xinping,Zhang, Changqing,Wu, Shang,Nothnagel, Eugene A.,Zhu, Jian-Kang Blackwell Publishing Ltd 2010 The Plant journal Vol.63 No.1

<P>Summary</P><P>Osmotic stress imposed by soil salinity and drought stress significantly affects plant growth and development, but osmotic stress sensing and tolerance mechanisms are not well understood. Forward genetic screens using a root-bending assay have previously identified <I>salt overly sensitive</I> (<I>sos</I>) mutants of Arabidopsis that fall into five loci, <I>SOS1</I> to <I>SOS5.</I> These loci are required for the regulation of ion homeostasis or cell expansion under salt stress, but do not play a major role in plant tolerance to the osmotic stress component of soil salinity or drought. Here we report an additional <I>sos</I> mutant, <I>sos6-1,</I> which defines a locus essential for osmotic stress tolerance<I>. sos6-1</I> plants are hypersensitive to salt stress and osmotic stress imposed by mannitol or polyethylene glycol in culture media or by water deficit in the soil. <I>SOS6</I> encodes a cellulose synthase-like protein, AtCSLD5. Only modest differences in cell wall chemical composition could be detected, but we found that <I>sos6-1</I> mutant plants accumulate high levels of reactive oxygen species (ROS) under osmotic stress and are hypersensitive to the oxidative stress reagent methyl viologen. The results suggest that SOS6/AtCSLD5 is not required for normal plant growth and development but has a critical role in osmotic stress tolerance and this function likely involves its regulation of ROS under stress.</P>

Apoptosis induction by alantolactone in breast cancer MDA-MB- 231 cells through reactive oxygen species-mediated mitochondrion-dependent pathway

Li Cui,Weiquan Bu,Jie Song,Liang Feng,Tingting Xu,Dan Liu,Wenbo Ding,Jianhua Wang,Changyang Li,Binge Ma,Yi Luo,Ziyu Jiang,Chengcheng Wang,Juan Chen,Jian Hou,Hong-mei Yan,Lei Yang,Xiao-bin Jia 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.3

Alantolactone is a sesquiterpene lactone isolatedfrom Inula helenium L. Although alantolactone possessesanti-inflammation and apoptosis-induction activities, theunderlying mechanism of anti-cancer effect on humanbreast cancer cells remains largely unknown. In this study,we explored the possibility of alantolactone as an apoptosis-inducing cytotoxic agent using MDA-MB-231 cells asin vitro model. Alantolactone significantly induced itsapoptosis, demonstrated by cell cycle analysis, annexinV-APC/7-AAD double staining and dUTP nick end labeling. Additionally, alantolactone triggered the mitochondrial-mediated caspase cascade apoptotic pathway, whichwas confirmed by increased Bax/Bcl-2 ratio, loss of MMP,release of cytc from mitochondria to cytoplasm, activationof caspase 9/3, and subsequent cleavage of PARP. Z-VADFMKpartially prevented apoptosis induced by alantolactone. Alantolactone provoked the production of ROS, whileNAC (a scavenger of ROS) reversed alantolactone-mediateddepolarization of MMP and apoptosis. Alantolactonemodulated the activities of MAPKs. As expected, cotreatmentwith SB203580, SP600125 or U0126 could reducedthe apoptotic rate. Furthermore, alantolactone decreasedthe protein expressions of p-NF-kB p65 and p-STAT3,increased p-c-Jun level in a dose-dependent manner. Thesefindings suggested that alantolactone possessed anticanceractivity via ROS-mediated mitochondrial dysfunctioninvolving MAPK pathway, and had an effect on the transcriptionfactors of NF-kB, AP-1 and STAT3.

Significance of Vesicle-Associated Membrane Protein 8 Expression in Predicting Survival in Breast Cancer

Mengci Yuan,Jianhua Liao,Ji Luo,Mengyao Cui,Feng Jin 한국유방암학회 2018 Journal of breast cancer Vol.21 No.4

Purpose: Vesicle-associated membrane protein 8 (VAMP8) is a soluble N-ethylmaleimide-sensitive factor receptor protein that participates in autophagy by directly regulating autophagosome membrane fusion and has been reported to be involved in tumor progression. Nevertheless, the expression and prognostic value of VAMP8 in breast cancer (BC) remain unknown. This study aimed to evaluate the clinical significance and biological function of VAMP8 in BC. Methods: A total of 112 BC samples and 30 normal mammary gland samples were collected. The expression of VAMP8 was assessed in both BC tissues and normal mammary gland tissues via a two-step immunohistochemical detection method. Results: The expression of VAMP8 in BC tissues was significantly higher than that in normal breast tissues. Furthermore, increased VAMP8 expression was significantly correlated with tumor size (p=0.007), lymph node metastasis (p= 0.024) and recurrence (p=0.001). Patients with high VAMP8 expression had significantly lower cumulative recurrence-free survival and overall survival (p<0.001 for both) than patients with low VAMP8 expression. In multivariate logistic regression and Cox regression analyses, lymph node metastasis and VAMP8 expression were independent prognostic factors for BC. Conclusion: VAMP8 is significantly upregulated in human BC tissues and can thus be a practical and potentially effective surrogate marker for survival in BC patients.

Ship Twin-propeller Jet Model used to Predict the Initial Velocity and Velocity Distribution within Diffusing Jet

Jinxin Jiang,Wei-Haur Lam,Yonggang Cui,Tianming Zhang,Chong Sun,Jianhua Guo,Yanbo Ma,Shuguang Wang,Gerard Hamill 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.3

The current research proposed the theoretical model for ship twin-propeller jet based on the axial momentum theory and Gaussian normal distribution. The twin-propeller jet model is compared to the more matured single propeller jet model with good agreement. Computational Fluid Dynamics (CFD) method is used to acquire the velocity distribution within the twin-propeller jet for understanding of flow characteristics and validation purposes. Efflux velocity is the maximum velocity within the entire jet with strong influences by the geometrical profiles of the blades. Twin-propeller jet model showed four-peaked profile at the initial plane downstream to the propeller compared to the two-peaked profile from a single-propeller. The four-peaked profile merges to be twopeaked velocity profile and then one-peaked profile due to the fluid mixing. Entrainment occurs between the ambient still water outside and the rotating flow within jet due to the high velocity gradient. The research proposes a twin-propeller jet theory with a serial of equations enabling the predictions of velocity magnitude within the jet.

Cyclic Shift Based Tone Reservation PAPR Reduction Scheme with Embedding Side Information for FBMC-OQAM Systems

( Yongpeng Shi ),( Yujie Xia ),( Ya Gao ),( Jianhua Cui ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.8

The tone reservation (TR) scheme is an attractive method to reduce peak-to-average power ratio (PAPR) in the filter bank multicarrier with offset quadrature amplitude modulation (FBMC-OQAM) systems. However, the high PAPR of FBMC signal will severely degrades system performance. To address this issue, a cyclic shift based TR (CS-TR) scheme with embedding side information (SI) is proposed to reduce the PAPR of FBMC signals. At the transmitter, four candidate signals are first generated based on cyclic shift of the output of inverse discrete Fourier transform (IDFT), and the SI of the selected signal with minimum peak power among the four candidate signals is embedded in sparse symbols with quadrature phase-shift keying constellation. Then, the TR weighted by optimal scaling factor is employed to further reduce PAPR of the selected signal. At the receiver, a reliable SI detector is presented by determining the phase rotation of SI embedding symbols, and the transmitted data blocks can be correctly demodulated according to the detected SI. Simulation results show that the proposed scheme significantly outperforms the existing TR schemes in both PAPR reduction and bit error rate (BER) performances. In addition, the proposed scheme with detected SI can achieve the same BER performance compared to the one with perfect SI.

Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer

Zhao Qiuyan,Zhu Zhonglin,Xiao Wenqin,Zong Guanzhao,Wang Chuanyang,Jiang Weiliang,Li Kai,Shen Jie,Guo Xingya,Cui Jianhua,Guo Lihong,Wan Rong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Pancreatic cancer (PC) is one of the most malignant tumors. Rapid progression and distant metastasis are the main causes of patient death. Hypoxia is a hallmark of multiple cancers and is involved in tumor biology. However, little is known about the roles of circRNAs in glycolysis and hypoxia-mediated progression of PC. Here, the expression pattern of hypoxia-related circRNAs was analyzed using RNA sequencing. A unique circRNA termed circRNF13 was found to be upregulated in PC tissues and may be a potential prognostic indicator. HIF-1α and EIF4A3 are involved in regulating the biogenesis of circRNF13. Furthermore, circRNF13 was validated to exert a stimulative effect on cell proliferation, angiogenesis, invasion and glycolysis. Importantly, we found that circRNF13 promoted PDK3 levels by acting as a miR-654-3p sponge, thus promoting the PC malignant process. Collectively, our results reveal that hypoxia-induced circRNF13 mediated by HIF-1α and EIF4A3 promotes tumor progression and glycolysis in PC, indicating the potential of circRNF13 as a prognostic biomarker and therapeutic target for PC.