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최연화,이영선,김홍빈,김치경,김봉수 대한감염학회 2001 감염 Vol.33 No.2
Background : Enterococci are important cause of nosocomial infections. Recently, vancomycin-resistant enterococci (VRE) has been increasingly reported as significant nosocomial pathogens. Therefore, accurate identification of enterococcal species is a prerequisite step for the appropriate antibiotic treatment and epidemiologic surveillance. We wanted to know the usefulness of PCR method compared with Vitek automatic identification system. Methods : Totally 105 isolates were identified on the species level by Vitek (GPI card and software version R06.1), methyl-α -D-glucopyranoside test, and PCR methods. Results : Among 105 enterococcal isolates, 59 were identified as E. faecium, 11 E. faecalis, 6 E. gallinarum by Vitek. But 29 isolates (28%) were unidentified. Subsequently all of these isolates were analyzed by PCR, the results of which were as follows : 17 E. faecium, 5 E. casseliflavus, 7 E. gallinarum. Two isolates identified as E. gallinarum by Vitek were reidentified as E. casseliflavus by PCR and other methods far phenotypic characterization. Conclusoin : PCR method was more accurate and sensitive than Vitek for the identification of enterococci species. (Korean J Infect Dis 33:123∼127, 2001)
한국인 제2형 당뇨병 환자에 있어 지속적 피하 인슐린 주입법 치료시 Lispro insulin와 Regular insulin의 효과 비교
유재등,박봉안,류하근,류주성,김정수,김치훈,박선민,최수봉 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-
Objective: The Lispro insulin has faster onset of effects and shorter duration than the human regular insulin, an analog of human insulin. The differences in the pharmacodynamics between the two types of insulin in the continuous subcutaneous insulin infusion(CSII) with insulin pump for treating the Korean Type 2 DM patients were analyzed. The effects on the regulation of blood sugar, the amount of insulin injected, and the frequency of hypoglycemia were compared between the lispro insulin and the regular human insulin. Method and Material: One hundred and three Korean Type 2 DM patients were randomly divided into two groups, 49 Lispro insulin and 54 Regular insulin.(Table 1) Both groups were treated for 14 days. Also at a set time before meal, insulin was injected as bolus, using a portable insulin pump, in both groups, 15 minutes before for lispro insulin and 30 minutes before for human insulin The HbA1C, C-peptide, AC/PC, height and weight were measured for each patients at the time of admission. The peripheral blood sugar level and insulin injection profile were recorded 7 times per day for each patients. Results: After treating for 7 days and 14 days, the blood sugar level normalized in both groups. There weren't any significant differences in blood sugar levels in both groups at point of the study, and also there weren't any differences in the frequency of hypoglycemia. In order to maintain the normalized blood sugar level, the lispro insulin group had higher total daily insulin requirement than the regular insulin group after 14 days of treatment (0.90±0.35 vs 0.73±0.39u/kg/day, p<0.05) This difference is probably due to the difference in basement insulin requirements rather than prandial insulin requirements (0.32±0.1 vs 0.43± 0.09u/kg/day, p<0.001). Discussion: In the hospitalized Korean Type 2 DM patients with CSII treatment, continuous subcutaneous insulin infusion therapy using lispro insulin has convenience in usage compared to the regular insulin. However, there were not any differences in the blood sugar levels. Also lispro insulin requires more insulin in order to maintain normal blood sugar level, which is probably due to the increase in the basal requirement. Also there were not any difference in the frequency of hypoglycemia.
Evaluation of Hydration Effect on Human Skin by $^1H$ MRS at 14.1T
Choi Chi-Bong,Hong Sung-Tak,Choe Bo-Young,Woo Dong-Chul,Yoon Seong-Ik,Cho Ji-Hyun,Lee Chul-Hyun,Cheong Chae-Joon,Park Sang-Yong,Oh Chil-Hwan Korean Magnetic Resonance Society 2006 Journal of the Korean Magnetic Resonance Society Vol.10 No.1
Purpose: We achieved high resolution MR imaging and spectra of human skin in vitro with using a 14.1 T MRI/MRS system, and evaluated the hydration effect of various cosmetic products by measuring the skin's. moisture concentration. Materials and Methods: We used the Bruker 14.1 T MRI/MRS system with a vertical standard bore that was equipped with a DMX spectrometer gradient system (200 G/cm at a maximum 40 A), RF resonators (2, 5 and 10 mm) and Para Vision software. Spin echo and fast spin echo pulse sequences were employed for obtaining the high resolution MR images. The 3D-localized point resolved spectroscopy (PRESS) method was used to acquire the MR spectra. Results: The high resolution MR images and spectra of human skin in vitro were successfully obtained on a 14.1 T system. The water concentration of human skin after applying a moisturizer was higher than that before applying a moisturizer. Conclusions: The present study demonstrated that the high-resolution MR images and spectra of human skin from a high field NMR instrument could be applicable to evaluating the hydration state of the stratum corneum.
Chi, Young Shik,Byon, Hye Ryung,Lee, Bong Soo,Kong, Bokyung,Choi, Hee Cheul,Choi, Insung S. WILEY-VCH Verlag 2008 Advanced functional materials Vol.18 No.21
<P>In this paper, we introduce the concept of a “polymeric ruler” for investigating distance-dependent emission behaviors of fluorophores, namely the quenching or enhancement of fluorescence, on flat Au surfaces in the range of 5 to ∼100 nm, which has not previously been easily accessible. The polymeric ruler is constructed by a highly controllable surface-initiated atom transfer radical polymerization of oligo(ethylene glycol)methacrylate (OEGMA), and the obtained thicknesses of the poly(OEGMA) (pOEGMA) layers range from ∼5 to ∼80 nm. The quenching or enhancement of fluorescence is found to be dependent upon the distance between fluorophores and the Au surface. In brief, fluorescence quenching occurs at distances within about 15 nm from the Au surface, and surface-enhanced fluorescence is observed at tens of nanometers beyond the range of quenching with the maximum enhancement at about 40–50 nm. The obtained information on the distance-dependent surface-enhanced fluorescence is applied to the construction of highly sensitive bioassay platforms: the use of the 50 nm thick pOEGMA layer lowers the detection limit up to 1 pM.</P> <B>Graphic Abstract</B> <P>The introduction of a polymeric spacer, or ruler, via a highly controllable surface-initiated atom transfer radical polymerization allows for the comprehensive investigation and the clear-cut understanding of distance-dependent emission behaviors of fluorophores on flat Au surfaces. The polymer layer can also be used for the construction of bioassay platforms based on plasmonic fluorescence enhancement. <img src='wiley_img/1616301X-2008-18-21-ADFM200800471-content.gif' alt='wiley_img/1616301X-2008-18-21-ADFM200800471-content'> </P>
Photodissociation effects on the electron density and on FTIR measurements in a BTMSM/Ar discharge
Bong Jung, Won,Jun Jang, Yong,Kyu Choi, Chi,Young Chang, Hong Institute of Physics Publishing Ltd. 2007 Journal of Physics. D, Applied Physics Vol.40 No.14
<P>For precise measurements of the absolute electron density in a bis-trimethylsilymethane (BTMSM) and argon plasma, the authors adapted a wave cutoff method and used the plasma frequency. The films were deposited on a p-type Si(1 0 0) substrate by means of UV-assisted inductively coupled plasma-enhanced chemical vapour deposition. Fourier transform infrared spectroscopy was used to investigate the bonding configuration of the SiCH films. With UV illumination, the electron density was found to increase in the low power region and decrease in the high power region. The peak intensity of Si-CH<SUB>3</SUB> decreases with UV illumination under all conditions. Moreover, when the UV source illuminates the plasma, the peak intensity of CH<SUB>2</SUB> increases whereas the peak intensity of CH<SUB>3</SUB> decreases.</P>
한치동(Chi Dong Han),정경재(Kyung Jae Jung),고석봉(Suk Bong Koh),최진석(Jin Suk Choi),조창호(Chang Ho Cho) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.10
Squamous cell carcinoma of the ovary is very rare malignant tumor originating from the malignant transfomation of mature cystic teratoma, usually being reported in about 1-3% of cases. Of malignant transformation, squamous cell carcinoma is the most common type and its prognosis is poor. Because of its rarity, the clinicopathologic characteristics are not well established. We have experienced a case of squamous cell carcinoma arising in mature cystic teratoma of ovary and present it with brief review of the literature.