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Yu, K.,Kang, S.,Park, N.,Shin, J.,Kim, Yangmee 부산대학교 유전공학연구소 2000 분자생물학 연구보 Vol.16 No.-
Mastoparan B (MP-B), an antimicrobial cationic tetradecapeptide amide isolated from the venom of the hornet Vespa basalis, is an amphiphilic x-helical peptide. MP-B possesses a mariety of biological activities, such as mast cells degradation histamine release, erythrocyte lysis and inhibition of the growth of Gram-positive and Gram-negative bacteria. In order to study the relationship between the structure and the biological activity of MP-B, we used four analogs by replacing amino acids with alanine. Tertiary structures of MP-B and its analogs in 2,2,2-trifluoroethanol (TFE)-containing aqueous solution have been determined by NMR spectroscopy and molecular modeling. The results indicate that [Ala^4]MP-B and [Ala^12]MP-B with higher hydrophobicity adopt a higher content of amphiphilic helical structures, and have better antimicrobial and hemolytic activities than MP-B. However, [Ala^3]MP-B and [Ala^9]MP-B and hydrophobicity have disordered structures. [Ala^3]MP-B and [Ala^9]MP-B have low antimicrobial activity and much less hemolytic activity relative to MP-B. It is likely that tryptophan residue in MP-B and appropriate hydrophobicity of MP-B to induce x-helical structure is essential for the antibacterial and hemolytic activity of MP-B. This study can aid understanding of the structure-activity relationship of MP-B and to design peptides to possess lytic activity.
Yu, K.,Park, K.,Kang, S.-W.,Shin, S.Y.,Hahm, K.-S.,Kim, Y. 부산대학교 유전공학연구소 2002 분자생물학 연구보 Vol.18 No.-
CRAMP was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. This peptide shows potent antimicrobial activity against gram-positive and gram-negative bacteria but no hemolytic activity against human erythrocytes. CRAMP was known to cause rapid permeabilization of the inner membrane of Escherichia coli. In this study, the structure of CRAMP adopts a mainly x-helical conformation in TFE/H_2O solution, DPC micelles, SDS micelles and liposomes, whereas it has a random structure in aqueous solution. The tertiary structure of CRAMP in TFE/H_2O (1:1, v/v), as determined by NMR spectroscopy, consists of two amphipathic α-helices from Leu^4 to Lys^10 and from Gly^16 to Leu^33. These two helices are connected by a flexible region from Gly^11 to Gly^16. Previous analysis of series of fragments composed of various portion of CRAMP revealed that an 18-residue fragment with the sequence from Gly^16 to Leu^33 was found to retain antibacterial activity. Therefore, the amphipathic x-helical region from Gly^16 to Leu^33 of CRAMP plays important roles in spanning the lipid bilayers as well as its antibiotic activity. Based on this structure, novel antibiotic peptides having strong antibiotic activity, with no hemolytic effect will be developed.
Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5
<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. Acta Neurol Scand: 2011: 123: 325–331. © 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>
Activities of Antioxidant and Redox Enzymes in Human Normal Hepatic and Hepatoma Cell Lines
Yuk-Young Lee,Hong-Gyum Kim,Haeng-Im Jung,Youn Hee Shin,Sung Min Hong,박은희,Jae Hoon Sa,Chang Jin Lim 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.2
The cellular defense system (including glutathione, glutathione-related enzymes, antioxidant and redox enzymes) plays a crucial role in cell survival and growth in aerobic organisms. To understand its physiological role in tumor cells, the glutathione con-tent and related enzyme activities in the human nor-mal hepatic cell line, Chang and human hepatoma cell line, HepG2, were systematically measured and com-pared. Superoxide dismutase, catalase, and glutathione peroxidase activities are 2.8-, 4.3-, and 2.9-fold higher in HepG2 cells than in Chang cells. Total glutathione content is also about 1.4-fold higher in HepG2, which is supported by significant increases in γ-glutamylcy-steine synthetase and glutathione synthetase activities. Two other glutathione-related enzymes, glutathione reductase and γ-glutamyltranspeptidase, are up-regulated in HepG2 cells. However, thioredoxin reduc-tase and glutathione S-transferase activities are sig-nificantly lower in HepG2 cells. These results propose that defense-related enzymes are largely modulated in tumor cells, which might be linked to their growth and maintenance.
Yuk, Soon Hong,Oh, Keun Sang,Cho, Sun Hang,Lee, Beom Suk,Kim, Sang Yoon,Kwak, Byung-Kook,Kim, Kwangmeyung,Kwon, Ick Chan American Chemical Society 2011 Biomacromolecules Vol.12 No.6
<P>We described the preparation of the glycol chitosan/heparin immobilized iron oxide nanoparticles (composite NPs) as a magnetic resonance imaging agent with a tumor-targeting characteristic. The iron oxide nanoseeds used clinically as a magnetic resonance imaging agent were immobilized into the glycol chitosan/heparin network to form the composite NPs. To induce the ionic interaction between the iron oxide nanoseeds and glycol chitosan, gold was deposited on the surface of iron oxide nanoseeds. After the immobilization of gold-deposited iron oxide NPs into the glycol chitosan network, the NPs were stabilized with heparin based on the ionic interaction between cationic glycol chitosan and anionic heparin. FE-SEM (field emission-scanning electron microscopy) and a particle size analyzer were used to observe the formation of the stabilized composite NPs, and a Jobin-Yvon Ultima-C inductively coupled plasma-atomic emission spectrometer (ICP-AES) was used to measure the contents (%) of formed iron oxide nanoseeds as a function of reaction temperature and formed gold deposited on the iron oxide nanoparticles. We also evaluated the time-dependent excretion profile, in vivo biodistribution, circulation time, and tumor-targeting ability of the composite NPs using a noninvasive NIR fluorescence imaging technology. To observe the MRI contrast characteristic, the composite NPs were injected into the tail veins of tumor-bearing mice to demonstrate their selective tumoral distribution. The MR images were collected with conventional T<SUB>2</SUB>-weighted spin echo acquisition parameters.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2011/bomaf6.2011.12.issue-6/bm200413a/production/images/medium/bm-2011-00413a_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bm200413a'>ACS Electronic Supporting Info</A></P>
Yuk, Heung Joo,Lee, Jae Won,Park, Hyun Ah,Kwon, Ok-Kyoung,Seo, Kyeong-Hwa,Ahn, Kyung-Seop,Oh, Sei-Ryang,Ryu, Hyung Won Elsevier 2017 Journal of Functional Foods Vol.34 No.-
<P><B>Abstract</B></P> <P>Few studies have reported on the antioxidant and anti-inflammatory properties of coumestrol (CM). In this study, CM not only significantly attenuated inflammation in acute lung injury (ALI) mice but also reduced the production of nitric oxide (NO), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and suppressed nuclear factor-kappaB (NF-κB) activation in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Interestingly, treatment with CM significantly attenuated the infiltration of inflammatory cells into the lung. CM decreased the levels of pro-inflammatory molecules, such as reactive oxygen species (ROS), TNF-α and IL-6, in the bronchoalveolar lavage fluid (BALF) of ALI mice. CM also inhibited the release of NO in the serum and reduced the expression of monocyte chemoattractant protein-1 (MCP-1) in the lung. In addition, CM suppressed the activation of NF-κB and the reduced expression of superoxide dismutase 3 (SOD3) in the lung. Our findings suggest that CM possesses protective effects in an ALI animal model.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Coumestrol attenuates the influx of inflammatory cells into the lung of ALI mice. </LI> <LI> Coumestrol inhibits the production of inflammatory mediators. </LI> <LI> Coumestrol suppresses the activation of NF-κB and the reduced expression of SOD3. </LI> <LI> Coumestrol has an anti-inflammatory activities in LPS-stimulated RAW264.7 macrophages. </LI> <LI> Coumestrol can develop as a therapeutic agent against ALI. </LI> </UL> </P>