유성견의 소구치 압하시 초기 치아 및 치주 조직의 조직학적 변화와 재생에 관한 연구

안병교,차경석,이진우 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.2

치아에 압하력을 elastic chain을 이용하여 지속적으로 교정력을 평균 9개월된 유 성견의 소구치에 가한 직후 치아와 그 주위 조직에서 일어나는 조직학적인 변화와 압하력을 제거한 후 일정기간이 지난 후 변화되었던 조직의 회복양상을 고찰하고, 이를 토대로 교정치료를 위한 생물학적인 근간을 마련하기 위하여 본 연구를 시행하였으며, Hematoxylin & Eosin 및 Masson's Trichrome염색법을 이용하여 치아와 주위조직의 반응을 치경부, 치근 중앙부, 치근단부로 나누어 광학 현미경하에서 관찰하여 다음과 같은 결론을 얻었다. 1. 대조군의 HE 소견에서 치주인대는 치근단부에서 치근경부까지 비교적 균일한 두께를 나타냈고, 치주인대의 주행방향은 치근경부에 수평 또는 약간 경사져 있었으며, 치근 중앙부와 치근단부에서는 사선으로 주행하였다. 2. 대조군의 MT 소견에서 치경부에서는 조골세포 및 파골세포가 나타났으며 치경부, 치근 중앙부 그리고 치근단부에 약한 골흡수와 신생골 침착이 보였다. 3. 실험1군에서는 치근단부에서 뚜렷한 파골세포의 증가와 혈관 충혈이 보였으며, 신생골 형성과 불규칙한 반전선들이 나타나 골흡후하는 양상을 보였다. 치주인대는 치경부에서 치근단에 이르기까지 뚜렷한 증가를 보였으며 치근단부에서 더 심하였다. 4. 실험 2군에서는 대조군에 비해 조골세포가 치근단부에서 혈관 충혈과 함께 여전히 관찰되었다. 치주인대 폭경은 치근단부에서 대조근에 비해서는 증가되었고 1군에 비해서는 감소된 양상을 보였다. 치주인대 주행방향은 1군과 같았고 단지 치근단부에서 혼합된 양상으로 관찰되었다. 이상과 같은 소견으로 보아 유성견 하악 소구치 압하시 초기 조직변화에서 압박을 받는 치근단부에서 파골세포의 뚜렷한 증가, 출혈상 및 간격확장, 그리고 골흡수와 백악질 흡수가 현저히 나타났으며, 치주인대 주행방향도 다양하였다. 또한 압하력을 제가한 후에 변형되었던 치아와 치아 주위조직들이 완전한 회복 양상을 보이지는 않았다. The purpose of this study was to investigate the initial tissue change, to repair on the teeth & surrounding tissue under the intrusive orthodontic forces by use of elastic chain, through the microscopic findings. For this study, three young adult mongrel dogs were used, and were divied into three group; the control group was delivered only casting crown, and the experimental group 1 was equipped with energy chain during 1 week, and experimental 2 group was delivered using energy chain during 1 week, and 3 weeks observation. All experimental groups and control groups were sacrificed to make the samples for microscopic findings on premolar teeth. All samples were examed and compared the histologic changes through the microscopic with H-E stain. The obtained results were as follows. 1. In hematoxylin-eosin stain of the control group, the periodontal ligament was constant width from apical third to cervical third of the root, and the periodontal fiber arrangement was horizontal or oblique in cervical third, oblique in middle and apical third of the root. 2. In Masson Trichrome stain of the control group, osteoblast and osteoclast appeared in cervical third of root, and bone resorption and new bone formation was observed in middle and apical third of the root. 3. In experimental 1, osteoclasts were increased highly, and hyperemia of blood vessels and new bone formation and bone resorption by reversal line in apical third of the root were seen. PDL width was increased apprarently from crest to apex of the root and more in apical third. 4. In experimental 2, osteoclasts and hyperemia of blood vessels were more increased than control material in apical third of the root. PDL width was increased more than control group in root apex, and was seen less than experimental 1. PDL arrangement was similar to experimental 1 and was mixed only in root apex. Therefore, in premolar intrusion of the young adult dog, there were increased osteoclast, hyperemia and dilation of blood vessel, resorption of alveolar bone and cementum, and different arrangement of PDL in initial tissue change. There was not observed complete repair after remove intrusive force.

다약제내성 발현 암세포에서 99mTc-sestamibi와 99mTc-tetrofosmin 섭취의 비교

유정아,정신영,서명랑,곽동석,안병철,이규보,이재태 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.3

목적 : 다약제내성이 유발된 암세포에서 ^99mTc-sestamibi와 ^99mTc-tetrofosmin의 암세포 내 섭취정도를 비교하고 다약제내성 극복제로 잘 알려진 verapamil 과 cyclosporin A 처리에 의한 두 방사성 의약품의 암세포 내 섭취정도를 비교해 보았다. 재료 및 방법 : Doxorubicin으로 다약제내성이 유발된 HCT15/CL02 대장암 세포와 doxorubicin과 vincristine으로 다약제내성을 유발시킨 K562(Adr)과 K562(Vcr) 백혈병 세포를 사용하였다. 다약제내성의 발현은 RT-PCR로 증명하였으며, vetapamil은 1, 10, 50, 100, 200 ㎛의 농도로, cyclosporin A는 0.1, 1, 10, 50, 100 ㎛의 농도로 각각 사용하였다. MIBI와 tetrofosmin의 암세포내 섭취는 37℃에서 1 × 10_6 cell/㎖ 농도의 단일세포 부유상태에서 1, 15, 30, 45, 60분 간격으로 배양하여 각 시간대별로 상층액과 침전물을 분리하여 각각의 방사능을 감마 계수기로 측정하였다. 결과 : 다약제내성이 발현된 암세포에서는 모세포에 비하여 MIBI와 tetrofosmin의 섭취가 감소되었다. 두 방사성약품의 섭취정도는 HCT15/CL02세포와 K562(Adr)세포에서는 유의한 차이가 없었으나, K562(Vcr)세포에서는 MIBI가 tetrofosmin보다 다소 높았다. Verapamil과 cyclosporin A를 처리하였을 때 MIBI와 tetrofosmin의 섭취율은 기저치보다 모두 증가하였고, verapamil에 의한 MIBI와 tetrofosmin의 섭취율(30분)을 기저치(30분)와 비교해 본 결과 HCT15/CL02세포에서 (100㎛)는 각각 11.9배와 6.8배, K562(Adr)세포에서(50 ㎛)는 각각 14.3배와 8배, K562(Vcr)세포에서(10㎛)는 각각 7배와 5.7배 증가하였다. Cyclosporin A에 의한 MIBI와 tetrofosmin의 섭취율(30분)을 기저치(30분)와 비교해 본 결과 HCT15/CL02세포에서(50 ㎛)는 각각 10배와 2.4배, K562(Adr)세포에서(50 ㎛)는 각각 44배와 13배, K562(Vcr)세포에서(10㎛)는 각각 18.8배와 11.8배 증가하여, MIBI의 섭취율이 tetrofosmin보다 1.2배에서 4배정도 높게 나타났다. 결론 : 이러한 결과로 보아 MIBI와 tetrofosmin은 다약제내성의 발현을 평가할 수 있는 방사성의약품으로 판단되며, 다약제내성 극복제의 효능평가에는 MIBI가 tetrofosmin보다 더 우수할 것으로 사료되나, 세포주에 따른 차이가 있을 수 있으므로 보다 많은 세포주에서의 추가적인 연구가 필요할 것이다. Purpose : Cellular uptakes of ^99mTc-sestamibi(MIBI) and ^99mTc-tetrofosmin into cancer cell lines expressing multidrug resistance(MDR) were investigated and compared. The effects of verapamil and cyclosporin A, well-known multidrug resistant reversing agents, on cellular uptake of both tracers were also compared. Materials and Methods : Doxorubicin-resistant HCT15/CL02 human colorectal cell and doxorubicin-resistant K562(Adr) and vincristine-resistant K562(Vcr) human leukemic cells were studied. RT-PCR analysis was used for the detection of mdr1 mRNA expression. MDR-reversal effects with verapamil and cyclosporine A were evaluated at different drug concentrations after incubation with MIBI and tetrofosmin for 1, 15, 30, 45 and 60 min, using single-cell suspensions at 1×10_6 cell/㎖ incubated at 37℃. Radioactivity in supernatants and pellets were measured with gamma well counter. Results : The cellular uptakes of MIBI and tetrofosmin in K562(Adr) and K562(Vcr) were lower than those of parental J562 cell. In HCT15/CL02 cells and K562(Adr) cells, there were no significant difference in cellular uptakes of both tracers, but cellular uptake of MIBI was higher than that of tefrofosmin in K562(Vcr) cells. Coincubation with verapamil resulted in a increase in cellular uptakes of MIBI and tetrofosmin. Verapamil increased cellular uptakes of MIBI and tetrofosmin by HCT15/CL02 cell by 11.9- and 6.8-fold, by K562(Adr) cell by 14.3- and 8-fold and by K562(Vcr) cell by 7- and 5.7-fold in maximum, respectively. Cyclosporin A increased cellular uptakes of MIVi and tetrofosmin by HCT15/CL02 cell by 11.9- and 6.8-fold, by K562(Adr) cell by 14.3- and 8-fold and by K562(Vcr) cell by 7- and 4.7-fold in maximum, respectively. Cyclosporin A increased cellular uptakes of MIBI and tetrofosmin by HCT15/CL02 cell by 10- and 2.4-fold, by K562(Adr) cell 44- and 13-fold and by K562(Vcr) cell by 18.8- and 11.8-fold in maximum, respectively. Conclusion : Taking together, MIBI and tetrofosmin are considered as suitable radiopharmaceuticals for detecting multidrug resistance. However, MIBI seems to be a better tracer than tetrofosmin for evaluation MDR reversal effect of the modulators. Since cellular uptakes of both tracers might differ in different cell types, further experiments regarding differences in cellular uptakes between cell types should be explored.

RFID/USN 기반에서의 mobile을 이용한 u-Yuhan 설계

안병도(Ahn byeong-do),양진호(Yang, jin-ho),안병태(Ahn byeong-tae),정범석(chung bhum-suk),이종하(Lee chong-ha) 한국콘텐츠학회 2007 한국콘텐츠학회 종합학술대회 논문집 Vol.5 No.1

현재 초고속으로 발전하는 유비쿼터스(ubiquitous) 환경에서 센서-Network를 기반으로 한 u-Campus구축이 발전하고 있다. 또한 PC, mobile등 그 어떤 정보기기라도 언제 어디서든 컴퓨팅이 가능하며 무선망의 발전으로 서비스 환경은 계속적으로 발전하고 있다. 본 논문에서는 mobile 활용을 이용한 사용자 환경에 적합한 u-Campus를 제안한다. u-Campus는 정보통신기술의 발전과 더불어 다양한 관련 기술이 교육에도 접목되어 활용되고 있다. 특히 유비쿼터스 컴퓨팅과 Network 기술을 대학의 캠퍼스에 접목시켜 구축한 새로운 형태의 캠퍼스가 u-Campus이다. 본 논문에서는 u-Yuhan 설계 방안을 제안하여 학생들의 효율적이고 향상된 학내 활동이 가능하도록 한다. In the ubiquitous which is rapidly developing with ultra speed thes days, constructing the u-Campus which is using the sensor-Network as its base is keep developing. Also, any information related equipments like PC and mobile, computing is possible whenever and wherever you want and due to the development of the wireless Network, the service environment is continually developing. In this article, I'd like to suggest the u-Campus which is very suitable to the user's environment which had used application of mobile. In u-Campus, various techniques are adopted and applied along with development of info-communication related technidques. Especially, the new type of campus which is constructed by adopting the ubiquitous computing net-work technique to the campus of university is the u-Campus. In this article, by suggesting u-Yuhan designing methods, more effective and advanced school activities of students to make possible.

First-Line chemotherapy with capecitabine and gemcitabine for advanced pancreatic cancer and clinical significance of CA19-9

( Byeong Bae Park ),( Joon Oh Park ),( Hyo Rak Lee ),( Hyun Jung Kim ),( Jung Mi Kwon ),( Sung Yong Oh ),( Jee Yun Lee ),( Ki Hyun Kim ),( Jin Seok Ahn ),( Won Seog Kim ),( Chul Won Jung ),( Young Suk 대한내과학회 2005 대한내과학회 추계학술대회 Vol.69 No.10

자살 기도자에 있어 유발요인, 위험도-구조가능도 접수와 생활의 스트레스에 관한 임상적 연구

안석철,기평석,연병길 大韓神經精神醫學會 1986 신경정신의학 Vol.25 No.3

The authors have studied the relationships between precipitating factors evaluated by the social readjustment rating scales, and risk scores, rescue scores, risk rescue scores of 295 suicide attempters who admitted to Han Gang Sacred Heart Hospital from Mar. 1981 to Jul. 1984. The results are as follows; 1) The most frequent precipiting factor of suicidal attempts classified by the social readjustment rating spouse(16.8) and next one was difficulty is friendship with opposite sex(12.7%) 2) There were no significant differences in risk scores, rescue scores, and risk-rescue scores between large life stress group and small stress group which were divided at the score of 300 in the social readiustment rating scale. 3) There were no significant differences in risk scores, recue scores, and risk-rescue scores among the categories of social readjustment rating scale. 4) The social readjustment rating scale was significantly correlated with rescue scores(p=0.045, Y=0.00198X+7.436) but not with risk and risk and risk-rescue scores.

Cone-beam computed tomography of mandibular foramen and lingula for mandibular anesthesia

Ahn, Byeong-Seob,Oh, Song Hee,Heo, Chong-Kwan,Kim, Gyu-Tae,Choi, Yong-Suk,Hwang, Eui-Hwan Korean Academy of Oral and Maxillofacial Radiology 2020 Imaging Science in Dentistry Vol.50 No.2

Purpose: The positions of the mandibular foramen (MnF) and the lingula affect the success rate of inferior alveolar nerve block. The objective of this study was to investigate aspects of the MnF and the lingula relevant for mandibular block anesthesia using cone-beam computed tomography (CBCT). Materials and Methods: Fifty CBCT scans were collected from a picture archiving and communications system. All scans were taken using an Alphard Vega 3030 (Asahi Roentgen Co. Ltd., Kyoto, Japan). Fifty-eight MnFs of 30 subjects were included in the study. The position of the MnF, the size of the MnF, the position of the lingula, the size of the lingula, and the shape of the lingula were measured and recorded. All data were statistically analyzed at a significance level of P<0.05. Results: The position of MnF was 0.1 mm and 0.8 mm below the occlusal plane in males and females, respectively. The horizontal position of the MnF was slightly anterior to the center of the ramus in males and in the center in females (P<0.05). The vertical position of the MnF was lower in females than in males(P<0.05). The MnF was an oval shape with a longer anteroposterior dimension. The height of the lingula was 9.3 mm in males and 8.2 mm in females. The nodular type was the most common shape of the lingula, followed by the triangular, truncated, and assimilated types. Conclusion: CBCT provided useful information about the MnF and lingula. This information could improve the success rate of mandibular blocks.

Effect of Polysaccharide of Fruiting Body of Pleurotus ferulae Mutant on the Oral Glucose Tolerance

Byeong-Kwon Ahn,Hyun-Sook Choi,Du-bok Choi,Wol-Suk Cha,Hoon Cho 한국식품영양학회 2015 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2015 No.08

Chronic administration of phosphodiesterase 5 inhibitor improves erectile and endothelial function in a rat model of diabetes

AHN, GOOK JUN,YU, JAE YOUNG,CHOI, SEUL MIN,KANG, KYUNG KOO,AHN, BYOUNG OK,KWON, JONG WON,KANG, SUNG KEUN,LEE, BYEONG CHUN,HWANG, WOO SUK Blackwell Science Ltd 2005 International journal of andrology Vol.28 No.5

<P>Summary</P><P>This study was conducted to determine if the long-term administration of the phosphodiesterase type 5 (PDE 5) inhibitor, DA-8159, to diabetic rats can ameliorate the development of erectile dysfunction (ED) and endothelial dysfunction. After inducing diabetes with streptozotocin, DA-8159 was orally administered at a dose of 3 mg/kg or 10 mg/kg for 8 weeks. To examine the effect on erectile response, electrostimulation of the cavernous nerve with the parameters of 3 V, 5 ms, 5 Hz or 10 Hz, was performed to measure the intracavernous pressure (ICP) and mean arterial pressure (MAP). Thoracic aorta relaxation <I>in vitro</I> was evaluated by adding acetycholine (Ach) cumulatively to the bathing medium. In addition, the plasma endothelin-1 (ET-1) levels were measured in order to investigate the effect of DA-8159 on endothelial dysfunction. The area under the curve (AUC) from the ICP/MAP ratio in the 10 Hz stimulation showed a significantly increased AUC after the 10 mg/kg treatment compared with the diabetic group (8891 ± 619 vs. 6316 ± 1016, respectively, <I>p</I> < 0.05). At the 5 Hz frequency, DA-8159 10 mg/kg also induced a significant increase in the AUC compared with the diabetic control. The maximum ICP/MAP ratio (%) of the 10 mg/kg treatment group was significantly higher in both the 10 Hz and 5 Hz frequency groups (<I>p</I> < 0.05). A treatment of 3 mg/kg tended to increase the AUC and peak ICP/MAP but was not statistically significant. The Ach EC<SUB>50</SUB> value of the diabetic group was significantly higher than in the normal control (120.50 ± 22.90 n<SMALL>M</SMALL> vs. 86.80 ± 9.30 n<SMALL>M</SMALL>, respectively), and 10 mg/kg treatment group showed a significantly lower EC<SUB>50</SUB> value (88.38 ± 19.7 n<SMALL>M</SMALL>). The ET-1 level was lower in groups treated with DA-8159, 3 mg/kg and 10 mg/kg treatment induced a statistical difference compared with the diabetic control (1.15 ± 0.34 fmol/mL vs. 2.51 ± 0.55 fmol/mL, respectively, <I>p</I> < 0.05). These results demonstrate that chronic administration of DA-8159 could attenuate the development of the ED in diabetes and its effect is associated with an improvement in the endothelial function.</P>

Repeated batch culture of Rhizopus arrhizus RA 128 using broken rice for L (+)-lactic acid production

( Byeong Kwon Ahn ),( Hyun Suk Choi ),( Ha Young Koh ),( Du Bok Choi ) 조선대학교 공학기술연구원 2014 공학기술논문지 Vol.7 No.1

The objective of this research was to study L (+)-lactic acid production by repeated batch culture of Rhizopus arrhizus RA 128 using broken rice as sole carbon source. The L (+)-lactic acid production increased with the increase of broken rice concentration by 60g/L. L (+)-lactic acid production was increased from 10.9 to 25.3g/L when pH was increased from 4.0 to 6.0. However, over 7.0 of pH, it was decreased. The L (+)-lactic acid productivity and cell concentration increased with the increase in temperature from 29 to 35 ℃. The maximum L (+)-lactic acid production and cell concentration was found with the 1.5% of CaCO3. Repeated batch culture was successfully performed for 4cycles for 12 days. All cycles showed that 234.5g/L of broken rice was consumed. 75g/L of total L (+)-lactic acid was obtained after 288hr. These results show that broken rice can be useful for the effective production of L (+)-lactic acid in the repeated batch culture of R. arrhizus RA 128.

α-Glucosidase and α-Amylase Inhibitory Activities of Hot Water Extract and Polysaccharide produced from Fermentation Broth of Pleurotus ferulae Mutant

Byeong-Kwon Ahn,Hyun-Sook Choi,Du-bok Choi,Wol-Suk Cha,Hoon Cho 한국식품영양학회 2015 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2015 No.08