Poster Session 1 : Analytical biochemistry Biochemical pharmacology and natural product chemistry Biotechnology ; 1-Palmitoyl-2-Linoleoyl-3-acetyl-glycerol isolated from deer antler, cervus nippon and its synthetic enantiomer potentiate the mitogenic acti

( Jeong Sook Suh ),( Soo Young Ko ),( Yun Jin Lee ),( Jin Kwon ),( So Yeop Han ),( Yoon Soo Bae ),( Jae Soon Eun ),( Jin Kyung Lhim ),( Gil Ja Jhon ) 생화학분자생물학회 2000 생화학분자생물학회 추계학술발표논문집 Vol.2000 No.-

어깨충돌증후군에 대한 침치료의 효과: 체계적 문헌고찰 및 메타분석

안정훈 ( Jeong Hoon Ahn ),배건희 ( Gun Hee Bae ),김병준 ( Byung-jun Kim ),박인화 ( In-hwa Park ),허인 ( In Heo ),차윤엽 ( Yun-yeop Cha ) 한방재활의학과학회 2024 한방재활의학과학회지 Vol.34 No.1

Objectives This review was conducted to evaluate the therapeutic effects of manual acupuncture (MA) for shoulder impingement syndrome (SIS). Methods We searched 12 electronic databases (DBpia, Korean studies Information Service System [KISS], Oriental Medicine Advanced Searching Integrated System [OASIS], Research Information Sharing Service [RISS], China National Knowledge Infrastructure [CNKI], CINAHL, Clinical Key, Cochrane Library, Embase, JAMA, PubMed, Web of Science) to find randomized-controlled clinical trials (RCTs) investigating therapeutic effects of MA for treating SIS. Shoulder Pain and Disability Index scores and numeric pain rating scale or visual analogue scale were analyzed as the main evaluation criteria. Results Among 181 studies, 169 were screened and only 12 RCTs were eligible in our review. Finally, 11 RCTs could be statistically analyzed. MA was more effective than sham treatment and physical therapy in terms of reducing pain (p=0.003, p=0.0007 each). Electroacupuncture (EA) showed more significant effect than physical therapy (PT) for improving shoulder pain (p<0.00001) and shoulder functionality (p<0.00001). Conclusions These results suggest that MA and EA could be superior option for treating SIS than sham treatment or PT. However this review has its limitations due to the small sample size and lack of well-designed RCTs that were included in the study. Further well-designed RCTs are necessary to provide high-level evidence. (J Korean Med Rehabil 2024;34(1):83-95)

위암 세포주에서 백화사설초의 세포자멸사 효과

박정배,성낙일,이준엽,허인경,김승민,남지형,이용국,최재혁,백진호,장태정,이종임,박건욱 동국대학교 의학연구소 2006 東國醫學 Vol.13 No.2

백화사설초는 한국과 중국에서 항암제로 이용되어온 약초이다. 이러한 백화사설초의 세포자멸사를 통한 항암 효과를 증명하기 위해 위암세포주인 SNU-719, 601, 668 각각에 백화사설초 메탄올 추출물로써 실험을 시행 하였다. 위암세포주 각각에 메탄올 추출물을 다양한 농도와 다양한 시간에 따라 실험 처치하여 세포 생존도를 측정하였다. 그리고 세포자멸사를 증명하기 위해 유세포분석을 통하여 subG1에 속하는 세포의 수를 계수하였고 Western blot 검사를 통해 caspase-3 enzyme의 발현을 조사하였다. SNU-719, 601 세포주에서는 메탄올추출물의 항암효과가 나타나지 않았고, SNU-668 세포주에서 항암효과가 나타났는데 메탄올 추출물 200과 400 ㎍/ml에서 농도에 비례하여 세포 생존도가 유의하게 감소하였다(0 ㎍/ml : 94.3土0.9%, 1 ㎍/ml : 95.8土0.8%, 5 ㎍/ml : 93.5土1.6%, 25 ㎍/ml : 95.3土2.0%, 50 ㎍/ml : 93.3土2.5%, 100 ㎍/ml : 93.5士3.8%, 200 ㎍/ml : 83.9土4.3%, 400 ㎍/ml : 72.8土8.4%). 100과 200 ㎍/ml, 200과 400 ㎍/ml 사이의 p-value는 각각 0.013, 0.005이었다. 그리고 메탄올 추출물 400 ㎍/ml을 SNU-668 세포주에 처리한 후 시간에 따른 세포 생존도를 조사한 결과 역시 시간의 증가에 따라 유의하게 감소하였다(0 hr. : 98.8土1.1%, 6 hr. ; 83.2土8.6%, 24 hr. : 37.6土8.3%, 48 hr. : 22.2土11.2%, p=0.019). 유세포분석상 subG1에 속하는 세포의 수는 농도와 시간에 비례하여 증가하였고 Western blot 검사상 caspase-3 proenzyme의 발현도 농도와 시간에 비례하여 감소하였다. 본 연구로 백화사설초의 메탄올 추출물이 위암세포주 SNU-668 세포자멸사에 효과가 있음이 증명되었다. 향후 백화사설초 메탄올 추출물의 성분분석과 세포자멸사 신호전달체계에 대한 연구를 통해 위암에 대한 새로운 항암물질 개발이 가능할 것으로 생각된다. Hedyotis diffusa has been used for cancer treatment in Korea and China. For the evaluation of anti-tumor activity through apoptosis, our experiments were performed with methanol extracts of Hedyotis diffusa on gastric cancer cell lines such as SNU-719, 601, 668. The cells were treated with various concentrations and periods of methanol extracts. Then, the cell viability was measured by trypan blue dye exclusion test. For the evaluation of apoptosis, we calculated the population of subG1 cells by the flow cytometry method, and examined the expression of caspase-3 by Western blot analysis. In SNU-719 and 601 cell lines, the anti-tumor activity of methanol extracts were not shown. However in SNU-668 cell lines, the anti-tumor activity was detected. The cell viability of SNU-668 was significantly decreased in a dose-dependent manner at 200 and 400 ㎍/ml of methanol extracts (0 ㎍/ml : 94.3±0.9%, 1 ㎍/ml : 95.8±0.8%, 5 ㎍/ml : 93.5±1.6%, 25 ㎍/ml : 95.3±2.0%, 50 ㎍/ml : 93.3±2.5%, 100 ㎍/ml : 93.5±3.8%, 200 ㎍/ml : 83.9±4.3%, 400 ㎍/ml : 72.8±8.4%). The p-values were 0.013 and 0.005 in between 100 ㎍/ml and 200 ㎍/ml, and 200 ㎍/ml and 400 ㎍/ml, respectively. The cell viability of SNU-668 was significantly decreased in a time-dependent manner as well (0 hr. : 98.8±1.1%, 6 hr. 83.2±8.6%, 24 hr. : 37.6±8.3%, 48 hr. : 22.2±11.2%, p=0.019). In the flow cytometry, the number of cells of subG1 population were increased in both dose-and time-dependent manners. In the Western blot analysis, caspase-3 proenzyme levels were decreased in both dose- and time-dependent manners as well. In conclusion, this study shows that methanol extracts of Hedyotis diffusa are effective on the apoptosis of SNU-668 gastric cancer cells. Therefore, through the analysis of components and the study of apoptotic signaling pathways of methanol extracts of Hedyotis diffusa, we could make a new drugs for the treatment of gastric cancer.

HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-b1

Ju Yeop Shin,Wonhee Hur,Jin Sang Wang,Jeong Won Jang,Chang Wook Kim,Si Hyun Bae,Sung Key Jang,Se-Hwan Yang,Young Chul Sung,Oh-Joo Kwon,Seung Kew Yoon 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.2

Liver cirhosis is one of the major complications of hepatitis C virus (HCV) infection, but the me-chanisms underlying HCV-related fibrogenesis are stil not clear. Although the roles of HCV core protein remain poorly understood, it is supposed to play an important role in the regulation of celular growth and hepatocarcinogenesis. The aim of this study was to examine the role of HCV established an in vitro co-culture system with primary hepatic stellate cel (HSC) isolated from rats, and a stable HepG2-HCV core cell line which had been transfected with HCV core gene. The expressions of fibrosis-related molecules trans-forming growth factor β1 (TGF-β1), transforming growth factor β receptor II (TGFβRII), α-smoth muscle actin (α-SMA) and conective tisue growth factor (CTGF) were analyzed via histo-pression levels of matrix metaloprotinase-2 (MP- 2) and colagen type I (Col I) from the co-cultured media were measured by zymogram and ELISA, respectively. The expresions of α-SMA, TGF-β1, Col I, TGFβRII and MP-2 were significantly in-creased in the co-culture of stable HepG2-HCV core with HSC. Moreover, the significant increases of CTGF and TGF-β1 in the HCV core- expresing cels were observed by either Northern or Wes-tern blot analysis. These results sugest that HCV core protein may contribute to the hepatic fibro-via up-regulation of CTGF and TGF-β1.

원통형 초발수 표면에서의 액적 충돌 시 스케일링 기법을 이용한 접촉 시간 예측

한정훈(Jeong Hoon Han),배창우(Chang Woo Bae),이동욱(Dong Wook Lee),김원정(Won Jung Kim),신승원(Seung Won Shin),남영석(Young Suk Nam),이충엽(Choong Yeop Lee) 대한기계학회 2019 대한기계학회 춘추학술대회 Vol.2019 No.4

Hepatitis C : Rate and predictors of treatment initiation for hepatitis C in Korea (초)

( Jong Yeop Kim ),( Ho Kil ),( Sook Hyang Jeong ),( Si Hyun Bae ),( Young Seok Kim ),( Han Chu Lee ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3(S)

Behavioral and Healthcare-Associated Risk Factors for Chronic Hepatitis C Virus Infection in Korea

Kim, Jong Yeop,Cho, Juhee,Hwang, Sung Ho,Kil, Ho,Bae, Si Hyun,Kim, Young Seok,Lee, Han Chu,Jeong, Sook-Hyang The Korean Academy of Medical Sciences 2012 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.27 No.11

<P>The risk factors related to hepatitis C virus (HCV) infection showed geographic and temporal differences. We investigated HCV-related risk factors in Korea where intravenous drug use (IVDU) is uncommon. The HCV-related risk factors were investigated in a prospective, multicenter chronic HCV cohort (n = 711) using a standardized questionnaire in four university hospitals. The results were compared with those of 206 patients with chronic liver diseases not related to either of HCV or hepatitis B virus infection (comparison group). The IVDU was found in 3.9% and remote blood transfusion (≥ 20 yr ago) in 18.3% in HCV cohort group, while that in comparison group was in none and 5.3%, respectively. In a multivariate logistic analysis, transfusion in the remote past (odds ratio [OR], 2.99), needle stick injury (OR, 4.72), surgery (OR, 1.89), dental procedures (OR, 2.96), tattooing (OR, 2.07), and multiple sexual partners (2-3 persons; OR, 2.14, ≥ 4 persons; OR, 3.19), were independent risk factors for HCV infection. In conclusion, the major risk factors for HCV infection in Korea are mostly related to conventional or alterative healthcare procedures such as blood transfusion in the remote past, needle stick injury, surgery, dental procedure, and tattooing although multiple sex partners or IVDU plays a minor role.</P>

Health Impact Assessment of Free Immunization Program in Jinju City, Korea

Kim, Keon-Yeop,Jeon, So-Youn,Jeon, Man-Joong,Lee, Kwon-Ho,Lee, Sok-Goo,Kim, Dong-Jin,Kang, Eun-Jeong,Bae, Sang-Geun,Kim, Jin-Hee The Korean Society for Preventive Medicine 2012 예방의학회지 Vol.45 No.4

Objectives: This study was conducted to assess the potential health impacts and improve the quality of the free immunization program in Jinju City by maximizing the predicted positive health gains and minimizing the negative health risks. Methods: A steering committee was established in September 2010 to carry out the health impact assessment (HIA) and began the screening and scoping stages. In the appraisal stage, analysis of secondary data, a literature review, case studies, geographic information systems analysis, a questionnaire, and expert consultations were used. The results of the data collection and analyses were discussed during a workshop, after which recommendations were finalized in a written report. Results: Increased access to immunization, comprehensive services provided by physicians, the strengthened role of the public health center in increasing immunization rates and services, and the ripple effect to other neighboring communities were identified as potential positive impacts. On the other hand, the program might be inaccessible to rural regions with no private clinics where there are more at-risk children, vaccine management and quality control at the clinics may be poor, and vaccines may be misused. Recommendations to maximize health gains and minimize risks were separately developed for the public health center and private clinics. Conclusions: The HIA provided an opportunity for stakeholders to comprehensively overview the potential positive and negative impacts of the program before it was implemented. An HIA is a powerful tool that should be used when developing and implementing diverse health-related policies and programs in the community.

회전 연료노즐의 분무특성 연구

이강엽(Kang-Yeop Lee),이동훈(Dong-Hun Lee),최성만(Seong-Man Choi),박정배(Jeong-Bae Park) 대한기계학회 2004 대한기계학회 춘추학술대회 Vol.2004 No.8

당뇨병이 없는 성인의 비만 여부에 따른 공복혈당장애 관련 요인

이수정(Su-Jeong Lee),김건엽(Keon-Yeop Kim),김명관(Myung-Gwan Kim),남행미(Hang-Me Nam),배상근(Sang-Geun Bae) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.6

본 연구는 정상 성인을 대상으로 공복혈당 장애 정도와 공복혈당 장애율과 관련된 요인을 파악하여 향후 당뇨병으로 진행하는 것을 예방하는데 도움을 주고자 실시 하였다. 연구 대상은 2014년 국민건강영양조사 자료 대상자인 7,550명 중 당뇨병이 있거나 무응답자를 제외한 20∼59세의 당뇨병이 없는 정상 성인 1,341명으로 하였다. 연구 방법은 대상자를 비만군과 정상 체중군으로 나누고, 인구학적 특성(성, 연령, 가구 소득, 교육 수준, 직업 등), 건강행태 특성(음주, 흡연, 걷기, 과일섭취 빈도 등), 비만도 및 공복혈당 장애 정도를 조사하였다. 연구결과 비만군 및 정상 체중군 모두 연령이 높은 경우 공복혈당 장애율이 유의하게 높았다 (p<0.001). 비만군에서는 과일섭취 빈도가 적은 경우와 음주를 많이 하는 경우 공복혈당 장애율이 유의하게 높았다 (p<0.05). 따라서 당뇨병이 없는 성인들의 경우 당뇨병으로 진행하는 것을 예방하기 위해 비만군과 정상체중군에 따른 접근 전략을 달리해야 하며, 특히 비만 성인을 대상으로 적절한 과일 섭취와 절주를 위한 교육 프로그램과 보건의료 서비스 정책이 필요하다. This study examined the prevalence of an impaired fasting glucose and related factors in normal non-diabetic adults. This study used the raw data (general characteristics, health behavior, obesity, impaired fasting glucose etc.) from the 6th Korea National Health & Nutrition Examination Survey (NHANES) in the second year (2014), and out of all 7,550 survey participants, 1,341 were selected as the final research subjects. After stratifying according to obesity, a logistic regression model was used to analyze the factors related to an impaired fasting glucose level. The older subjects were more likely to have an impaired fasting glucose in both the obesity group and normal weight group (p<.001). The obesity group showed a higher impaired fasting glucose rate when they drank more alcohol (p<.05) and consumed less fruit (p<.05). Therefore, balanced nutrients and control of the blood sugar level through proper education and public health policies offruit intake and alcohol are needed.