DMNQ S-64 Induces Apoptosis via Caspase Activation and Cyclooxygenase-2 Inhibition in Human Nonsmall Lung Cancer Cells

LIM, E.-S.,RHEE, Y.-H.,PARK, M.-K.,SHIM, B.-S.,AHN, K.-S.,KANG, H.,YOO, H.-S.,KIM, S.-H. Wiley (Blackwell Publishing) 2007 Annals of the New York Academy of Sciences Vol.1095 No.1

<P>Shikonin has been reported to induce apoptosis and inhibit angiogenesis in vivo and in vitro. 6-(1-propoxyiminoalkyl)-5,8-dimethoxyoxy 1,4-naphtoquinone S-64 (DMNQ S-64) was synthesized as a shikonin derivative. In this article, the underlying apoptotic mechanism of DMNQ S-64 was examined. DMNQ S-64 exerted cytotoxicity against A549 lung carcinoma cells with IC(50) of 27.3 microM. Apoptotic bodies were observed in DMNQ S-64-treated A549 cells by 4'-6-diamidino-2-phenylindole (DAPI) staining assay. DMNQ S-64 also increased sub-G1 DNA portion in a concentration-dependent manner by flow cytometric analysis. Western blotting has revealed that DMNQ S-64 effectively activates the expression of caspase 8, 9, and 3, cleaves poly (ADP-ribose) polymerase, and increases the ratio of Bax/Bcl-2. Furthermore, cytochrome c was released in a concentration-dependent manner by DMNQ S-64. Similarly, DMNQ S-64 significantly increased caspase 3 activity by enzyme-linked immunosorbent assay (ELISA). It also significantly inhibited the level of prostaglandin E2 (PGE(2)) by ELISA and downregulated the expression of cyclooxygenase-2 (COX-2) in a concentration-dependent manner. Taken together, DMNQ S-64 may exhibit cytotoxicity against A549 cells via caspase activation and COX-2 inhibition.</P>

RF Interference Analysis for KOMPSAT-II Spacecraft

Lim, S.B.,Kim, D.Y.,Chang, Y.K. 통신위성우주산업연구회 2001 Joint Conference on Satellite Communications Vol.2001 No.-

In general, LEO satellite uses S-band communication link for the whole satellite management, whereas the satellite payload frequently implements X-band and/or Ku-band transmitter for payload data transmission. The transmission system should have out-band rejection filter (protection circuit) to prevent any interference with the satellite receiver, so that the receiver can be normally operated. It is usually implemented in the transmitter itself but the vendor of a transmitter could not take it into account whole out of band frequency because a variety of individual satellites utilize different RF communication systems. The KOMPSAT-II, which is an earth observation satellite, is being developed by Korea Aerospace Research Institute and Korean industries. It has S-band communication system for the bus and X-band transmission system for payload data. In addition, L-band GPS Receiver is being incorporated to achieve the KOMPSAT-II mission goal. Unfortunately, the KOMPSAT-2 payload system does not have external out-band rejection~ filter nor transmitter for S-band and L-band protection. The transmitter only has band rejection filter for TDRSS. In addition, it has high gain directional antenna whose directional beam has to pass over the S-band antenna. In this paper, the analysis method is described to calculate the interference effects of the S-band spurious noise power on X-band transmitting system to S-band receiver. The S-band antenna is positioned very closely to the X-band Antenna by I .2m. We will also introduce general simple RF interference analysis method applicable to a traditional system, including advantage and disadvantages. The X-band transmitting system is often required to add the specified filter to suppress the S-band spurious signal for the safety of the receiver performance. The analysis result shows that the performance of S-band receiver is satisfied with no implementation of the specified filter.

Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome

Lee, J.Y.,Kang, C.S.,Beom, H.R.,Jang, Y.R.,Altenbach, S.B.,Lim, S.H.,Kim, Y.M.,Park, C.S. Academic Press 2017 Journal of cereal science Vol.73 No.-

<P>DH2O, a new wheat mutant missing low-molecular weight glutenin subunits encoded by the Glu-B3 locus, was discovered among double haploid lines obtained from a cross between the Korean wheat cultivars Keumkang and Olgeuru. Absence of the Glu-B3 LMW-GS proteins was determined by one-dimensional gel electrophoresis (SDS-PAGE) and confirmed by two-dimensional gel electrophoresis (2-DGE) and tandem mass spectrometry (MS/MS). The deletion of Glu-B3 genes was also demonstrated using allele specific DNA markers. Basic agronomic traits, protein content, dough mixing properties and bread loaf volume of DH2O and parental wheat cultivars were evaluated in field-grown wheat over a two year period. This mutant is a valuable resource for understanding the function of individual LMW-GS alleles or genes at the Glu-B3 locus using either breeding or biotechnology approaches. (C) 2017 Elsevier Ltd. All rights reserved.</P>

Molecular characterization and expression analysis of IκB from Haliotis discus discus

Kasthuri, S.R.,Whang, I.,Navaneethaiyer, U.,Lim, B.S.,Choi, C.Y.,Lee, J. Academic Press 2013 Fish & shellfish immunology Vol.34 No.6

Innate immune system relies on the recognition of pathogen associated molecular patterns present in the microbes by the pattern recognition receptors leading to the activation of signaling cascade and subsequent synthesis of cytokines. NF-κB is a major stimulus activated transcription factor, which regulates the expression of a diverse array of genes. IκB is an inhibitor of NF-κB, retaining NF-κB in an inactive state in the cytoplasm. In this study, we have reported the characterization of first abalone IκB (HdIκB). The cDNA possessed an ORF of 1200 bp coding for a protein of 400 amino acids with molecular mass of 45 kDa and isoelectric point of 4.7. HdIκB protein possessed a conserved phosphorylation site <SUP>58</SUP>DSGIFS<SUP>63</SUP> in the N-terminal region, six ankyrin repeats, and a PEST sequence in the C-terminal region. A casein kinase II phosphorylation site could also be observed in the PEST sequence. Constitutive expression of HdIκB revealed its physiological significance since NF-κB is known to be activated by various stimuli. Elevated expression of HdIκB transcripts could be observed in abalones challenged with various mitogens and live microbes. This novel characterization of abalone IκB would further be a positive approach in the affirmation of evolutionary conservation and significance of this protein as a repressor/inhibitor of a pleiotropic transcription factor like NF-κB.

Influence of spherical particles and interfacial stress distribution on viscous flow behavior of Ti@?Cu@?Ni@?Zr@?Sn bulk metallic glass composites

Hong, S.H.,Kim, J.T.,Mun, S.C.,Kim, Y.S.,Park, H.J.,Na, Y.S.,Lim, K.R.,Park, J.M.,Kim, K.B. Elsevier Applied Science 2017 INTERMETALLICS Vol.91 No.-

Bulk metallic glass composites containing micro-scale B2 particles are subject to investigation with regards to the influence of B2 particles and interfacial stress and strain distribution on the viscous flow behavior at a super-cooled liquid state. An increased volume fraction of B2 particles leads to an increase of minimum viscosity and influences viscous flow behavior before crystallization. In high temperature deformation, the bulk metallic glass shows homogeneous deformation feature. However, the heterogeneous deformation feature is found in thermoplastically deformed bulk metallic glass composite. The strong stain accumulation and sluggish viscous flow occur around B2 particles, which are caused by the heterogeneous stress distribution linked to stress concentration and shear stress impediment around B2 particles. The sluggish viscous flow of super-cooled liquids around B2 particles during high temperature deformation induces an increase of viscosity and strongly affects the viscous flow behavior of Ti-based bulk metallic glass composites containing micro-scale spherical B2 particles.

HIV 감염진단 표준화를 위한 정도관리(Quality Assurance) 프로그램 개발

이주실,박근용,김성순,강춘,김옥진,서순덕,박혜경,최병선,남정구,도경미,구본기,김현숙,임종백,조권,황유성,황병갑,이용래 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Characteristics of the antimicrobial resistance of Staphylococcus aureus isolated from chicken meat produced by different integrated broiler operations in Korea

Kim, Y. B.,Seo, K. W.,Jeon, H. Y.,Lim, S. K.,Lee, Y. J. Oxford University Press 2018 Poultry science Vol.97 No.3

<P>Vertical integration of the broiler industry allows producers to combine different biosecurity and sanitation practices, housing technologies, and feeding regimens to improve food safety. The purpose of this study was to investigate the prevalence of Staphylococcus aureus (S. aureus) and to characterize the antimicrobial-resistant isolates recovered from 7 different integrated broiler operation systems in Korea. Among 200 chicken meat samples, 94 were observed to be positive for S. aureus. However, the prevalence varied from 25.0 to 58.3% in chicken meats, indicating variation in S. aureus occurrence among the operations. Four methicillin-resistant S. aureus isolates (MRSA) were recovered from 3 different operations. A high proportion of the S. aureus isolates were resistant to penicillins (51.2%), tetracycline (38.8%), and ciprofloxacin (CIP; 33.9%). Especially, 3 different operations showed a high number of CIP resistance (45.5 similar to 100%) and multidrug resistance (50.0 similar to 100%). Among 41 CIP-resistant S. aureus isolates, 75.6% showed a double amino-acid exchange of both gyrA and parC, with CIP minimum inhibitory concentrations (MIC) of >= 32 mu g/mL. Four MRSA isolates showed resistance to 5 or 7 classes of antimicrobial agents, exhibiting oxacillin, CIP, and enrofloxacin MIC ranges of 16 to 128, 32 to 64, and 8 to 128 mu g/mL, respectively, and had double mutations of S84L/S80F in gyrA/parC. Our findings suggest that S. aureus with resistance to important antimicrobial compounds can now be found in association with integrated broiler operations, providing the data to support the development of a monitoring and prevention program in integrated operations.</P>

상업적 응유효소의 탈지유에 대한 단백질 분해 작용

신현수,김상범,임종우 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.6

상업적 단백질 분해 효소에 0.02% CaCl_2를 첨가하여 응유 활성화를 시킨 탈지유에 대한 분해 작용의 결과를 요약하면 다음과 같다. 다양한 효소별 가수분해 시간에 따른 가수분해도는 미생물 유래 효소와 trypsin은 pepsin과 papain W-40보다 높은 분해도를 나타냈다. 12% TCA 용액에 가용성인 NPN의 양은 trypsin이 가장 높은 분해도를 나타내었고 rennet과 pepsin이 가장 낮은 분해도를 보였다. 전기영동에 있어서 trypsin과 protease S는 α-lactalbumin을 분해하였고 papain w-40은 β-lactoglobulin을 미약하게 분해하였으며 neutrase 1.5는 90분 이후부터 α-lactalbumin과 β-lactoglobulin을 분해하였다. Rennet과 비교한 전기영동상에서는 rennet에 의해 분해 되지 않은 α_s-casein과 β-casein을 trypsin과 protease S가 다량 분해하였고 k-casein은 rennet에 비해 papain W-40이 상당 수준의 분해상을 나타내었다. 이상의 결과 가수분해도 및 NPN 양은 trypsin, neutrase 1.5 및 protease S가 다른 효소에 비해 높게 나타났으며, 전기영동상에서는 pepsin과 neutrase 1.5가 rennet과 유사한 경향을 나타내었다. Proteolytic activities of some commercial milk clotting enzymes(rennet, trypsin, pepsin, papain W-40, neutrase 1.5 and protease S) in bovine skim milk containing 0.02% CaCl_2 were determined by measuring DH(Degree of Hydrolysis), NPN(Non Protein Nitrogen) and by comparing patterns of SDS-PAGE(Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis). The DH of microbial enzymes(neutrase 1.5 and protease S) and trypsin in bovine skim milk were higher than those of pepsin and papain W-40. The amounts of NPN in the milk treated with trypsin and the other animal enzymes(rennet and pepsin) showed the highest and lowest degrees of proteolysis, respectively. SDS-PAGE showed that trypsin and protease S hydrolyzed α-lactalbumin and papain W-40 hydrolyzed β-lactoglobulin slightly, while neutrase 1.5 hydrolyzed both α-lactalbumin and β-lactoglobulin after treating for 90 min. Trypsin and protease S easily hydrolyzed α_s-casein and β-casein, which were not hydrolyzed by rennet. Papain W-40 hydrolyzed k-casein more than rennet as shown in SDS-PAGE. Based on the results of the experiments, the DH and NPN of trypsin, neutrase 1.5 and protease S were shown to be higher than those of the other enzymes. The SDS-PAGE patterns of papain W-40 and neutrase 1.5 were similar with that of rennet.

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

Paenibacillus harenae sp. nov., isolated from desert sand in China

Jeon, C. O.,Lim, J.-M.,Lee, S. S.,Chung, B. S.,Park, D.-J.,Xu, L.-H.,Jiang, C.-L.,Kim, C.-J. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.1

<P>A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain B519T, was isolated from a desert sand sample of Gansu Province, China. Strain B519T was strictly aerobic and cells were motile by means of peritrichous flagella. The strain grew optimally at 32-35 degrees C and pH 6.5-7.0. Chemotaxonomic data supported the affiliation of the new isolate to the genus Paenibacillus, including menaquinone-7 (MK-7) as the major isoprenoid quinone, DNA G+C content of 49.9 mol%, cell-wall type A1gamma (meso-diaminopimelic acid as the diagnostic diamino acid) and anteiso-C(15 : 0), iso-C(15 : 0), C(16 : 0) and iso-C(16 : 0) as the major fatty acids. Comparative 16S rRNA gene sequence analysis showed that strain B519T was most closely related to Paenibacillus alkaliterrae KSL-134T (98.0 % similarity). DNA-DNA relatedness between strain B519T and P. alkaliterrae KSL-134T was about 12.3 %. On the basis of phenotypic characteristics and molecular properties, strain B519T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus harenae sp. nov. is proposed. The type strain is B519T (=KCTC 3951T =DSM 16969T).</P>