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KLF10 inhibits CYP450 to reduce TAA-induced hepatic injury
Azra Memon,Yuri Seo,Joo-Yong Lee,Woon Kyu Lee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Kruppel-Like Factor 10 (KLF10), an important transcription factor for hepatic glucose and lipid metabolism thought to be linked with liver injury and repair. However, it is not clear if KLF10 plays a role in reducing injury or activating repair in TAA-induced liver injury. Also, the molecular mechanism of KLF10 in the liver injury model is largely unknown. Here, we demonstrated that TAA-induced liver toxicity was significantly increased in KLF10 knockout mice. Strikingly, 70% of KLF10-/- mice die within 48 hours after TAA 300 mg/kg/BW treatment that generally known as a safe dose to induce liver injury without notable lethality. Histological analysis of TAA-treated liver of KLF10 knockout mice revealed a significant increase of TUNNEL positive shrunk hepatic nuclei, characteristics of pyknosis, inflammation, and massive hemorrhage. Also, biochemical analysis revealed KLF10 knockout mouse experienced a significant amount of oxidative stresses after TAA treatment evidenced by the increase of malondialdehyde, a well-known oxidative stresses marker, and the decrease of glutathione, the most abundant cellular thiol antioxidant, in TAA-treated KLF10-/- mouse correlated with an increased protein level of iNOS. For the mechanistic understanding of the protective role of KLF10 against TAA-induced liver toxicity, we showed KLF10 negatively regulates CYP450, a key enzyme to convert nontoxic TAA into toxic metabolites TAASO and TASO2 that triggers necrosis and liver injury. Our findings suggested a protective role of KLF10 against TAA-induced liver injury and provided a unique angle to understand the liver injury.
Characterization of the porcine Nanog 5’-flanking region
Azra Memon,Ki-Duk Song,Woon-Kyu Lee 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3
Objective: Nanog, a homeodomain protein, has been investigated in humans and mice using embryonic stem cells (ESCs). Because of the limited availability of ESCs, few studies have reported the function and role of Nanog in porcine ESCs. Therefore, in this study, we investigated the location of the porcine Nanog chromosome and its basal promoter activity, which might have potential applications in development of ESCs specific marker as well as understanding its operating systems in the porcine. Methods: To characterize the porcine Nanog promoter, the 5'-flanking region of Nanog was isolated from cells of mini-pig ears. BLAST database search showed that there are two porcine Nanog genomic loci, chromosome 1 and 5, both of which contain an exon with a start codon. Deletion mutants from the 5'-flanking region of both loci were measured using the Dual-Luciferase Reporter Assay System, and a fluorescence marker, green fluorescence protein. Results: Promoter activity was detected in the sequences of chromosome 5, but not in those of chromosome 1. We identified the sequences from –99 to +194 that possessed promoter activity and contained transcription factor binding sites from deletion fragment analysis. Among the transcription factor binding sites, a Sp1 was found to play a crucial role in basal promoter activity, and point mutation of this site abolished its activity, confirming its role in promoter activity. Furthermore, gel shift analysis and chromatin immunoprecipitation analysis confirmed that Sp1 transcription factor binds to the Sp1 binding site in the porcine Nanog promoter. Taken together, these results show that Sp1 transcription factor is an essential element for porcine Nanog basal activity the same as in human and mouse. Conclusion: We showed that the porcine Nanog gene is located on porcine chromosome 5 and its basal transcriptional activity is controlled by Sp1 transcription factor.
Azra Memon,Yuliya Pyao,Yerin Jung,Hwa-Sik Choi,송기덕,이운규 한국유전학회 2021 Genes & Genomics Vol.43 No.4
Background Krüppel–like factor 10 (KLF10) belongs to the Sp1-like transcription factor family, which plays an important role in many directions, e.g., cell proliferation, apoptosis, and diferentiation. Its 5′ upstream regions are conserved across mammalian species. However, the regulatory mechanism has not been elucidated yet. Objective Nonetheless the basal transcriptional regulation mechanisms of these regions are unknown. Here, we characterized it which is indispensable for the basal transcription of the Klf10 gene. Methods Seven deletions of 5′ upstream DNA fragments from the 10 kb mKlf10 genomic DNA were produced by PCR and cloned into the upstream of the luciferase (Luc) reporter gene in the pGL3 basic plasmid. Result The luciferase reporter assay showed that the DNA sequence at positions from −101 to +68 was required for a principle activity in the promoter of mKlf10 gene, in which transcriptional factor binding motifs, one JunB and two Sp1 sites, are included. Mutations at the sequence of JunB motif, but not at the two Sp1, abrogated the promoter activity completely, suggesting the indispensable role of JunB site for basal transcription of mKlf10 gene. Moreover, electrophoretic mobility and supershift assays (EMSA) uncovered that JunB protein bound to this region specifcally. Conclusion Taken together, our study revealed that the JunB but not Sp1 at mKlf10 promoter functions as a positive basic factor for the transcriptional activity of the gene.
이운규,최진영,김용배,Azra memon,전기홍 (사)한국조리학회 2020 한국조리학회지 Vol.26 No.2
In order to find out the effects of shelf-life extending, Campbell grapes were treated with phytoncide pads and compared it with control and other treatments like sulfur dioxide acid pads. With using the phytoncide pad derived from pine tree extract, the pattern of fragrance components according to the storage period of Campbell grape was analyzed by GC-MSD electronic nose. The proper selection of qualitative sensors (peaks) used in the multi-variate chemical analysis of electronic noses distinguished the pattern of fragrance components of electronic noses over the storage period of grapes. In addition, the results of PCA and DFA analysis showed that the GC-MSD electronic nose can distinguish the difference according to the storage period. The ethanol from GC-MSD increased until 6 days of storage and then decreased from 9 days of storage, and by treatment, phytoncide pad 2 treatment showed a pattern of fragrance components with extended shelf life.
Multiple Hypercoagulability Disorders at Presentation of Non-Small-Cell Lung Cancer
이정민,유정선,임준혁,김정수,박지선,Azra Memon, B.S.,이슬기,남해성,조재화,곽승민,이홍렬,김현정,홍근정 대한결핵및호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.77 No.1
Hypercoagulability disorders are commonly encountered in clinical situations in patients with a variety of cancers. However, several hypercoagulability disorders presenting as first symptoms or signs in cancer patients have rarely been reported. We herein described a case of a woman with adenocarcinoma of the lung presenting with deep vein thrombosis, nonbacterial thrombotic endocarditis, recurrent cerebral embolic infarction, and heart failure.
CASE REPORT : Multiple Hypercoagulability Disorders at Presentation of Non-Small-Cell Lung Cancer
( Jeong Min Lee ),( Jun Hyeok Lim ),( Jung Soo Kim ),( Ji Sun Park ),( Azra Memon ),( Seul Ki Lee ),( Hae Seong Nam ),( Jae Hwa Cho ),( Seung Min Kwak ),( Hong Lyeol Lee ),( Hyun Jung Kim ),( Geun Jeo 대한결핵 및 호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.77 No.1
Hypercoagulability disorders are commonly encountered in clinical situations in patients with a variety of cancers. However, several hypercoagulability disorders presenting as first symptoms or signs in cancer patients have rarely been reported. We herein described a case of a woman with adenocarcinoma of the lung presenting with deep vein thrombosis, nonbacterial thrombotic endocarditis, recurrent cerebral embolic infarction, and heart failure.
Multiple Hypercoagulability Disorders at Presentation of Non-Small-Cell Lung Cancer
Lee, Jeong Min,Lim, Jun Hyeok,Kim, Jung-Soo,Park, Ji Sun,Memon, Azra,Lee, Seul-Ki,Nam, Hae-Seong,Cho, Jae-Hwa,Kwak, Seung-Min,Lee, Hong Lyeol,Kim, Hyun-Jung,Hong, Geun-Jeong,Ryu, Jeong-Seon The Korean Academy of Tuberculosis and Respiratory 2014 Tuberculosis and Respiratory Diseases Vol.77 No.1
Hypercoagulability disorders are commonly encountered in clinical situations in patients with a variety of cancers. However, several hypercoagulability disorders presenting as first symptoms or signs in cancer patients have rarely been reported. We herein described a case of a woman with adenocarcinoma of the lung presenting with deep vein thrombosis, nonbacterial thrombotic endocarditis, recurrent cerebral embolic infarction, and heart failure.