Quantitative comparison of mRNA expression of glucosyltransferase(GTF) between xylitol-resistant(X^(R)) and xylitol-sensitive(X^(s)) mutans streptococci

Kim, Chong-Chul,Lee, Mi-Na,Kim, Young-Jae,Lee, Sung-Hoon 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Since the long-term exposure of mutans streptococci to xylitol is known to select for xylitol-resistant(X^(R)) natural mutants, the occurrence and survival of such X^(R) strains were performed in batch culture methods. The aim of the study was to compare the differentiation and quantification of mRNA expression of the gtf genes of X^(R) and X^(S) mutans streptococci. Using a real-time reverse-transcription polymerase chain reaction, the expression of each gtf was determined. In X^(R) strains, the relative levels of transcription of gtfB and gtfC were decreased while that of gtfD was increased, suggesting the presence of independent promoters. It also suggested that mutation related to production of glucosyltransferase occurred under the exposure of xylitol could explain the caries-preventive mechanisms of xylitol. 자일리톨에 장기간 노출된 mutans streptococci는 자일리톨에 내성이 발현되어 자일리톨 내성균주가 생성된다고 알려져 있다. 본 연구의 목적은 mutans streptococci에서 자일리톨 내성균주와 감성균주의 gtf 유전자 발현량을 각각의 유전자별로 정량적으로 분석하고 비교하는 것이다. 실시간 역전사 중합효소연쇄반응법을 이용하여 각각의 gtf 발현을 조사한 결과 gtfD는 증가한 반면. gtfB와 gtfC는 감소하였는데 이는 각 유전자의 독립된 조절기전이 존재함을 보여주는 것이다. 또한 자일리톨에 노출된 mutans streptococci에서의 glucosyltransferase와 연관된 유전자변형이 자일리톨의 치아우식증 예방효과의 작용기전 중 하나임을 알 수 있었다.

大田市 下水道計劃에 關한 硏究

宋錫哲,李鍾珏 大田工業高等專門學校 1968 論文集 Vol.3 No.-

This thesis is, as the first step in establishing the sewerage plan, the report of studying the problems and devices on the sewerage planning in future by means of investigating and analysing these streams, through which the filthy water flows. In Taejon stream, flowing through the center of the city, and Dae Dong stream which flows front the east of it, the centaminating of them is so extreme that I am going to present the following devices in a respect that we should consider a counter-measure of purifying these streams in order to prevent it. The problems and devices of a sewerage planning are like these: 1. To make the draining smooth by means of completing the sewerage systems in the whole city. 2. To eliminate the filthy water by establishing the intercepting sewer on stream street. 3. To set up a treatment division of industrial waste water. 4. To make designers replace the water carrage toilets with flush ones in constructing of them, when they received permission for building houses. 5. To protect the region of the downstream from the damage of filthy water, by establishing sewerage treatment plants. By carrying out the above mentioned five projects urgently, I think that we are able to obtain the cheerfulness and the sound sanitation of the city.

集團力學과 易理論

李種鐵 공주교육대학교 초등연구원 1978 公州敎大論叢 Vol.14 No.2

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하이드록시프로필화 찹쌀 전분의 이화학적 성질 및 유과제조 특성

유철,최현욱,김종태,김동섭,최성원,박영준,백무열 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

近代 化學肥科商品의 展望

夫鍾哲 제주대학교 1969 논문집 Vol.1 No.-

In the agricultural business, fertilizer which is a basic producer's good, occupies a very important position. There are basically two kinds of fertilizer, the barn-yard type and the many various artificial manures now being marketed. But, as far as quality and excellence are concerned, the chemical fertilizers are the best. The old adage of "There is no agriculture without fertilizer" has been updated to "there is no modern agriculture without chemical fertilizer". Fertilizer, besides being used to cultivate the necessary foods such as corn, race, etc, is valuable also as a "growthaide" for luxury plants-tea, tobacco-trait trees, etc. The study of chemical fertilizer can be looked at from two sides. On the one hand, there is the position of the consumer who requires only a study of the quality of fertilizer. On the other hand, there is the position of the producer who demands a study of the techniques of production, cost-price analysis, regulation of supply and demand, and the development of the market.

鹽分最小感應値와 血壓의 聯關性에 關한 疫學的 硏究

趙聲鍾,宋東彬,車喆煥 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.3

High blood pressure has become a common finding in Korean population and a major public health problem leading to an increased mortality and morbidity in recent years. With this accumulating evidence has come increasing incentive to find safe and simple methods for preventing and controlling hypertension. Reduction of dietary salt has been advocated by some for such method. The effect of dietary salt intake to hypertension however is still not clear and it is a matter of controversy particulary on whether the method of reducing dietary salt would be effective in controlling the hypertension of the general population. The purpose of this study was to investigate this controversial matter by assessing the degree of association between dietary salt intake and blood pressure using the method of detecting salt recognition threshold in general population. A total of 680 rural and urban inhabitants were studied from June 1984 to January 1985. The result of study is as followings; 1. The relationship between salt recognition, threshold and optimal gustation of salt was observed to be high in pilot study with a correlation coefficient of 0.729 (p<0.001). 2. In hypertensive group, mean systolic and diastolic blood pressures (BP) were significantly higher in those having high salt recognition threshold (SRT) than those of low SRT. The same evidence could be observed neither in normotensive nor in total population groups. That is, in hypertensive group, the mean systolic BP for those of high SRT was higher than those of low SRT by 8.8 mmHg in male and 8.8mmHg in female, and the mean diastolic BP for those of high SRT was also higher than those of low SRT by 4.3 mmHg in male and 8.5mmHg in female respectively. 3. There was no significant difference in the prevalence rate of hypertension among those of high SRT compared with those of low SRT. 4. In multiple regression analysis using age, body mass index (weight/hight²), socioeconomic status, amount of alcohol consumption, amount of cigarettee smoking, family history of hypertensi no and salt recognition threshold as independent variables, the multiple correlation coefficients turned out to be from 0.36 to 0.47 indicating that these variables explain 13 to 22 percent of the variances of systolic and diastolic pressures. The variables showing relatively higher beta coefficients were age, body mass index and alcohol consumption in male, where as the body mass index, age and family history were the corresponding variables in female. The relationship of salt recognition threshold to blood pressure was turned out to be very low. These findings indicate that dietary salt intake would have a significant effect on blood pressure in hypertensive group (may include the salt sensitive individuals), while no relationship could be observed in normotensive group.

Achondroplasia환아의 치의학적 소견에 대한 증례보고

김종철,전현철 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.2

Achondroplasia is characterized by undergrowth of long bones and cartilages and by low height. The name of this disease had been called classical chondrodystrophy, dyschondroplasia, chondrodystrophy and micromelia before Parrot reported the term of this disease as Achondroplasia. We are reporting in this paper on an 3-year and 4-month-old boy with achondroplasia. The dental findings are as follows : 1. Open bite and relatively mandibular prognathism were observed in the front teeth. 2. The size, number, shape, structure of the teeth were usual and the tongue was a little bigger than the normal. 3. The metacarpal bones were short and broadening. Mesial, distal border of the prox. phalanges were irregular and also trabecular pattern was abnormal in the hand-wrist P-A view. 4. According to lat. cephalometric analysis, the cranial basis was underdeveloped.

과잉치에 의한 정중이개와 치아회전의 치료 증례

김종철,이광수 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.2

The supernumerary teeth are defined as the teeth that are excess of the normal number. Especially, mesiodens refers to a supernumernary tooth that is present in the midline of the anterior maxilla. Such supernumerary teeth can casue midline diastema. The diastema is defined as a space greater than 0.5 millimeter between the proximal surfaces of adjacent teeth. Diastemas usually are a normal stage in the development of the dentition, specially between the ages of 7 and 12 years. Usually these diastemas close completely after eruption of permanent maxillary canines. But sometimes diastemas remain unclosed. Therefore differential diagnosis in necessary between physiologic diastema and pathologic one. The patient, 8-year-old boy, with the chief complain of midline diastema and excessive teeth rotation visited the department of pediatric dentistry of seoul national university hospital. The supernumerary teeth were removed surgically. The midline diastema and teeth rotation were treated by the fixed orthodontic applinace. And then frenectomy, fibrotomy and gingivoplasty were performed. The periodic recall check and comprehensive orthodontic treatment are recommended.

J.-J. Rousseau의 자서전 서술방식에 관하여 : 언술행위 이론을 중심으로

이신철,박종탁,양인봉 東亞大學校 大學院 1990 大學院論文集 Vol.15 No.-

En nous situant au discours vu dans la perspective de l'ennonciation, nous nous proposons, dans notre etude, d'analyser les quelques phrases ou le sujet evoque sa naissance et les relations qu'elle fait entre lui et ses parents. Pour cela, nous nous servons volontiers de procedures linguistiques qui dependent de la notion d'enonciation. A travers la tension entre les protagonistes, telle qu'au moment de l'ecriture le narrateur la restitue, nous pouvons constater que l'ambivalance du pere se refletent dans la double attitude de son fils : au-dela de la tendresse filiale apparaissent l'accusation et la haine, c'est ce qui nous amene donc a croire que c'est le d'esir inconscient du narrateur qui a distribue les roles a l'un rt a l'autre. en nous referant a l'opposition entre temps histor que et temps du discours, nous constatons aussi que le narrateur rapporte un enenement historique, mais en realite cette objectivite n'est qu' apparente. ciest parce que l'evocation subite de la mort de son pere revele la vengeance secrete du narrateur. La conclusions que nous pouvons tirer de notre etude, c'est que notre etude n'est qu'une approche pour atteindre les traces du sujet inconscient qui affleure dans les textes autobiographiques.

土壤 環境에 따른 內生菌根의 生態學的 硏究

金鍾均,金俊泰,鄭炳哲 公州大學校 基礎科學硏究所 1995 自然科學硏究 Vol.4 No.-

VAM과 포자 밀도와 토양 온도, pH, 수분함량과 인 함량은 각각 음의 상관 관계에 있고 유기물 함량과 총 질소량은 각각 양의 상관 관계에 있다. 遷移 안정 지역인 非攪亂地에 비해 攪亂地의 植生에서 VAM 공생이 높았다. The environmental factors of soil affecting on the density of VAM spore were temperature, pH, moisture and phosphorus, which were negative factors, and organic compound and nitrogen were positive. In succession-stability area, the floral zone of disturbance area was higher VAM simbion than non-disturbance area.