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넙치 (Paralichthys olivaceus) 뇌로부터 phospholipase D 활성 억제 단백질의 정제 및 특성 규명
서정수,김은희,황은영,김남득,김동선,이형호,정준기,SEO Jung-Soo,KIM Eun-Hi,HWAWG Eun-Young,KIM Nam Deuk,KIM Dong Sun,LEE Hyung-Ho,CHUNG Joon-Ki 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4
본 연구에서는 넙치, Pafalichthys olivaceus 뇌 조직에서 인지질가수분해효소 D (phospholipase D, PLD) 활성의 특성 규명 및 이 활성을 억제하는 단백질을 분리 정제하여 그 특성을 규명하였다. 넙치 뇌 조직에서 PLD 활성이 관찰되었으며, 이 활성은 포스파티딜 이노시톨 비스인산염 (phosphatidyl-inositol 4,5-bisphos-phate, $PIP_2$)에 대해서 의존성을 나타내었으나, ADP-rebosylation factor (ARF)에 의해서는 영향을 받지 않았다. PLD 억제물은 넙치 뇌 조직의 세포질 분획물을 사용하여 여러 종류의 칼럼을 통하여 분리 정제하였고, 그 억제물의 분자크기 및 기작의 특성을 규명하였다. 마지막 chromatography을 통하여 여섯 개의 억제를 나타내는 분획물을 얻었으며, 이 중 두 개의 분획물인 IIA IIB는 $PIP_2$-phosphatase activities를 나타내었다. 이 중 IIA 분획물은 면역화학적 분석을 통하여 inositolpolyphosphate 5-phosphatase family로 알려져 있는 신경말단 단백질인 synaptojanin으로 동정되었다. 그리고 IIB fraction은 Superose 12 gel filtration chromatography을 통하여 158-kDa의 크기로 확인되었으며, 이것은 면역화학적 분석을 통하여 synaptojanin과는 별개의 단백질로 판명되었다. 또한, IIB 분획물은 $PIP_2$ phosphatase activity 확인 실험에서 대사산물로서 phosphatidylinositol phosphate (PIP)만을 생성하였다. 이 결과는 IIB 분획물이 $PIP_2$의 4혹은 5 위치의 인산 (phosphate) 중 어느 하나만을 선택적으로 가수분해시킨다는 것을 암시한다. 이상의 연구 결과들을 종합하여 보면, 넙치 뇌 조직에는 다양한 형태의 $PIP_2$-phosphatases가 존재하며, 이들은 $PIP_2$-의존적인 PLD 활성의 억제조절과정에서 중요한 역할을 할 것으로 사료된다. Flounder brain cytosol contains protein inhibitors that markedly inhibit the activity of partially purified brain membrane phospholipase D (PLD) which is dependent on phosphatidylinositol 4,5-bisphosphate ($PIP_2$) but insensitive to ADP-ribosylation factor (ARF), The PLD inhibitors have been enriched through several chromatographic steps and characterized with respect to size and mechanism of inhibition. Sequential chromatography of the brain cytosol yielded six inhibitor fractions, Two (IIA and IIB) of six inhibitor fractions showed the $PIP_2$-phosphatase activities. IIA was identified as synaptojanin, a nerve terminal protein that has known to be a member of the inositolpolyphosphate 5-phosphatase family, by immunoblot analysis. IIB showed an apparent molecular mass of 158 kDa by Superose 12 gel filtration chromatography and was immunologically distinct from synaptojanin. IIB hydrolyzed $PIP_2$, yielding only phosphatidylinositol phosphate (PIP) as product, suggesting that IIB hydrolyzes only one phosphate from either the 4- or 5-position of PI (4,5)$P_2$. These studies demonstrate that the existence of multiple $PIP_2$-phosphatases have been implicated in the negative regulation of $PIP_2$-dependent PLD activity within flounder brain.
경구투여에 의한 양식산 넙치내 Erythromycin의 약물동태학적 특성 분석
서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),정승희(Sung-Hee JUNG),박명애(Myung-Ae PARK),김나영(Na-Young KIM) 한국수산해양교육학회 2014 水産海洋敎育硏究 Vol.26 No.2
The pharmacokinetics of erythromycin (EM) after oral administration was studied in the cultured olive flounder, Paralichthys olivaceus, using LC/MS/MS. After single- or multiple-dose administration of EM (50, 100, 200 ㎎/㎏ body weight and 50 ㎎/㎏ for 5 days) by oral route in olive flounder (350±40 g, 22±0.5℃), the concentration in the serum was determined at 1, 3, 6, 9, 24, 72, 120, 168, 264, 360, 504 and 720 h post-dose. The kinetic profile of absorption, distribution and elimination of EM in serum were analyzed fitting to a two-compartment model by WinNonlin program. The area under the concentration-time curve (AUC), maximum concentration (Cmax), time for maximum concentration (Tmax) following oral administration of 50, 100 and 200 ㎎/㎏ b.w. and 50 ㎎ for 5 days. EM was 165.3 hr<SUP>*</SUP>㎍/㎖ (Cmax, 34.63 ㎍/㎖; Tmax, 1.56 hr), 212.8 hr<SUP>*</SUP>㎍/㎖ (Cmax, 60.38 ㎍/㎖; Tmax, 3.99 hr), and 592.37 hr<SUP>*</SUP>㎍/㎖ (Cmax, 63.01 ㎍/㎖; Tmax, 4 hr), respectively. The results of this study related to dosage and μㆍwithdrawal times could be used for prescription of EM in field for the treatment of bacterial diseases in olive flounder.
Florfenicol 경구 투여에 따른 조피볼락 혈액의 약동학적 특성
서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG) 한국수산해양교육학회 2020 水産海洋敎育硏究 Vol.32 No.3
The pharmacokinetics characterization of florfenicol(FF) after single oral medication was studied in the cultured korean rockfish, Sebastes schlegelii, using high performance liquid chromatography(HPLC). After single oral medication of FF(20 ㎎/㎏ body weight) in the cultured Korean rockfish(590±50 g, 23±1.5℃), the FF concentration in the serum was determined at different period(1, 5, 10, 15, 24, 30, 50 and 168 h post dose). The pharmacokinetics profile of FF oral medication in serum were analyzed to a one- or two-compartment model by WinNonlin program, respectively. Under the one-compartment model, the area under the concentration-time curve(AUC), maximum concentration(Cmax) and time for maximum concentration(Tmax) were 31.84 ㎍·h/㎖, 1.14 ㎍/㎖, and 9.3 h, respectively. Under the two-compartment model, the AUC, Cmax and Tmax were 45.52 ㎍·h/㎖, 1.14 ㎍/㎖ and 9.43 h, respectively. The results of this study could be used for determining efficacy dosage and withdrawal times of FF for the therapy of bacterial diseases in korean rockfish.
생약제 고삼 뿌리 열수추출물의 넙치 투여시 질병 저항성에 미치는 영향
서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG),김진도(Jin-Do KIM),정승희(Sung-Hee JUNG),김나영(Na-Young KIM),지보영(Bo-Young JEE),박명애(Myoung-Ae PARK) 한국수산해양교육학회 2015 水産海洋敎育硏究 Vol.27 No.6
The effects of lightyellow sophora, Sophora flavescens extract were tested on non-specific immune response and a disease resistance of olive flounder, Paralichthys olivaceus. For feeding trial, weight gain of fish fed different treated groups were not significant differences. Serum analysis of treated and control group did not demonstrated any differences. Lysozyme activity in the kidney and spleen of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase, respectively. In addition, phagocytic activity of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase compared to the control group. Histopathology of the liver and kidney tissue of the administrated group for 4<SUP>th</SUP>, 8<SUP>th</SUP> and 12<SUP>th</SUP> week showed no particular signs of tissue degeneration. The treated group was higher than control group by analyzing the relative percent survival (RPS) of the experimental challenge of Edwardsiella tarda. Therefore, the lightyellow sophora showed effective in preventing fish-pathogenic edwardsiellosis for a certain period of time.
서정수(Jung Soo SEO),이지훈(Ji-Hoon LEE),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),황지연(Jee Youn HWANG),황성돈(Seong Don HWANG),정지민(Ji Min JEONG),심재동(Jae-Dong SHIM) 한국수산해양교육학회 2019 水産海洋敎育硏究 Vol.31 No.6
This research has studied current situation of aquatic medicine use of nine major aquaculture species in our nation from year 2016 to 2017. We have proceeded the research after selecting each species of domestic licensed aquaculture farms which used stratification structure samples(Flounder, 60 of sampling aquaculture farms/527 of total aquaculture farms; Rockfish, 63/819; Red sea bream, 60/463; Starry flounder, 37/79; Israeli mirror carp, 42/108; Eel, 57/360; Rainbow trout, 47/150; Whiteleg shrimp 51/209; Abalone 65/1,295). Classification of aquatic medicine research targets are divided into four big groups depending on their functional characteristics such as antibiotics, parasiticide, disinfectants and others(digestive medicine etc). We have visited each sampling aquaculture farms to check the amount of aquatic medicine that they have used. And the result came out in the order of Flounder> Rockfish> Red sea bream, Eel, Starry flounder> Rainbow trout> Israeli mirror carp, Abalone, Whiteleg shrimp. Overall, the future research needs to be done at least more than 3 years of research on the domestic aquaculture farms for the comparison between aquatic medicine use and aquaculture production of each species.
넙치에 대한 Erythromycin thiocyanate의 단일 근육투여에 따른 약물동태학적 특성
서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON) 한국수산해양교육학회 2018 水産海洋敎育硏究 Vol.30 No.6
The study was aimed at investigating the pharmacokinetics of erythromycin thiocyanate(EM) in the cultured olive flounder(Paralichthys olivaceus) following single intramuscular administration, using LC/MS/MS following. After single administration of EM(5 and 10 mg/kg body weight) by intramuscular injection in olive flounder(332±20 g, 20±0.5℃), the EM concentration in the serum was determined at 1, 3, 6, 9, 24, 72, 120, 168, 264, 360, 504 and 720 h post-dose, respectively. The kinetic profile of EM intramuscular administration in serum were analyzed fitting to a non-compartment model by WinNonlin program. The residual pattern of EM was immediately decreased on after single administration. Concentrations of EM were not measurable at 168 hours (7 days) after intramuscular administration in all samples. The results of this study could be used for determining withdrawal time and efficacy dose of EM for the treatment of bacterial diseases in culturing olive flounder.
제주 및 완도지역 양식넙치에서 잔류 동물용의약품 모니터링
서정수(Jung Soo SEO),조희성(Hee Sung JO),정아름(Ah-Reum JEONG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),황지연(Jee Youn HWANG),황성돈(Seong Don HWANG),정지민(Ji Min JEONG),이지훈(Ji-Hoon LEE) 한국수산해양교육학회 2019 水産海洋敎育硏究 Vol.31 No.5
Residue levels in randomly collected olive flounder muscles were monitored in 2015-2018. Fish were obtained from fish farms located in Jeju and Wando regions to monitor 45 veterinary antibiotic drugs. For analysis, sensitive methods which adopt simultaneously detection of multiple agents were used: high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) detection. A total of 1,200 samples were analyzed, and antibiotic residues were detected in 15 Jeju samples (1.25%) and 45 Wando samples (3.75%). None of them contained residues exceeding respective maximum residue limits (MRLs) set by the Korean Food Code. Although the present monitoring result identifies safe status overall, current surveillance efforts over antibiotic use need to be maintained by continuous monitoring.
서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jun ),정승희 ( Sung Hee Jung ),김명석 ( Myoung Sug Kim ),박명애 ( Myoung Ae Park ),이철호 ( Chul Ho Lee ),한명철 ( Myoung Chul Han ),김진우 ( Jin Woo Kim ),지보영 ( Bo Young Jee ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1
The infestation status of anisakid type larvae was investigated in migrating chum salmon (Oncorhynchus keta), with different condition (captured area, sex, body portion) during 2006~2008. The mean infection number of anisakid larvae per individual female and male fish captured from Namdae river was 98±27, 103±27, respectively. The mean infection number of anisakid larvae per individual female and male fish captured from the coastal area of Yangyang was 63±18 and 108±17, respectively. The anisakid larvae were mainly found in abdominal muscles (85%) but only a little in the visceral portion. Two types of anisakid larvae (A. simplex, Contracaecum type) were identified but other anisakid larvae were not detected. To investigate the effect of storing temperature on the viability of anisakid larvae, the section of abdominal muscle were stored at different temperature (room temperature, 4℃, -20℃, -80℃). As a result, it was necessary to store at -20℃ for more than 6 hrs to kill the larvae. The present results revealed that chum salmon caught in Korea are heavily infected with anisakid larvae, mainly in the abdominal muscle, and A. simplex was dominantly found in this study.
서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG) 한국수산해양교육학회 2018 水産海洋敎育硏究 Vol.30 No.6
Scuticociliate are one of the main parasite affecting the flounder aquaculture industry, causing significant economic losses in Korea. Aquatic formalin has been approved to treat against external parasitic infestation in flounder aquaculture pond. To investigate metabolic effect after treating the parasitic agent aquatic formalin in scuticociliate Miamiensis avidus, the transcriptome analysis was conducted. Through exposing with low concentration(10~50 ppm) of formalin for 96 hr, parasite trancriptomes were sequenced cDNA libraries in the GX Plex sequencer. A total of million read for control and formalin treated group were assembled in 187,210 and 177,959 contigs, respectively. After normalization and assembling with house keeping gene(β-actin), transcripts were assigned to biological processes and functions after annotation in Gene Ontology(GO). Specifically, 10 exclusive transcripts were up- or down-regulated for control and formalin treated group, respectively. Our results provide valuable genetic information for further analysis of the biological responses mechanism of aquatic formalin exposure in M. avidus.