보중익기합대칠기탕(補中益氣合大七氣湯) 추출물의 유전독성 평가

황희정,변준석,허진일,Hwang, Hui-Jeung,Byun, Joon-Seok,Heo, Jin-Il 대한한의학방제학회 2006 大韓韓醫學方劑學會誌 Vol.14 No.1

The genotoxicity of water extract of Bojungikkeehapdaechilki-tang was tested by In Vitro Chromosome Aberration Test. Bacterial Reverse Mutation Assay and Micronucleus test according to OECD Guidelines and KFDA Guidelines. The obtained results were as follows : 1. Chromosome Aberration Test: In Vitro Chromosome Aberration Test of Bojungikkeehapdaechilki-tang extracts was carried out using cultured Chinese hamster lung cells in the presence and absence of metabolic activation system(S-9 mix). No significant changes in the number of aberrant metaphases having structural and number of aberrations were detected in Bojungikkeehapdaechilki-tang extracts treated groups. 2. Bacterial Reveres Mutation Assay: Bojungikkeehapdaechilki-tang extracts was evaluated for its potential to induce reverse mutation in the histidine auxotroph strains of Salmonella typhimurium such as TA100, TA1535, TA98 and TAl537 and the tryptophan auxotroph strain of Escherichia coli WP2 uvrA. No significant changes in the number of revertant colonies compared to its negative control were detected in Bojungikkeehapdaechilki-tang extracts treated groups against all 5 strains. 3. Micronucleus test: Micronucleus test of Bojungikkeehapdaechilki-tang extracts were performed using specific pathogen free 7-week old male ICR mouse. No significant changes in the number of micronucleated polychromatic erythrocytes among 2000 polychromatic erythrocytes compared to negative control were detected in all Bojungikkeehapdaechilki-tang extracts treated groups. In summarized above-mentioned results, it is concluded that Bojungikkeehapdaechilki-tang extracts have not genotoxicity against In Vitro Chromosome Aberration Test, Bacterial Reverse Mutation Assay and Micronucleus test.

반하사심탕(半夏瀉心湯)이 Doxorubicin에 의해 유발(誘發)된 간장(肝臟) 및 비장(脾臟) 독성(毒性)에 미치는 영향(影響)

황희정,신민규,곽민아,이윤희,김상찬,변준석,Hwang, Hui-Jeung,Shin, Min-Kyu,Kwak, Min-Ah,Lee, Yun-Hee,Kim, Sang-Chan,Byun, Joon-Seok 대한암한의학회 2002 大韓癌韓醫學會誌 Vol.8 No.1

Object The effect of Banhasasim-tang extracts on the hepatic, splenic toxicity and induced by Doxorubicin administration(Three injection protocol) were monitored using male ICR mice. Method The changes of body weigh, organ weights of liver and spleen were observed with blood GOT and GPT level. Results 1. Increase of absolute and relative liver weight observed in Doxorubicin treatment group were dose-dependently inhibited by Banhasasim-tang extracts. In addition, the degrees of liver congestion and necrotic spot were significantly and dose-dependently decreased after Banhasasim-tang extracts dosing groups compared to that of Doxorubicin treatment group. It is also demonstrated that elevated serum GOT and GPT levels in Doxorubicin treatment group were significantly decreased in Banhasasim-tang extracts dosing groups. 2. Decrease of absolute and relative spleen weight observed in Doxorubicin treatment group were dose dependently inhibited by Banhasasim-tang extracts. In addition, the degrees of splenic atrophy were significantly and dose-dependently decreased after Banhasasim-tang extracts dosing groups compared to that of Doxorubicin treatment group.

동원(東垣) ${\ll}$비위론(脾胃論)${\gg}$ 수록(收錄) 처방(處方)의 치법(治法)에 대(對)한 문헌적(文獻的) 고찰(考察)

이윤희,황희정,김상찬,박순달,변준석,Lee, Yun-Hee,Hwang, Hui-Jeung,Kim, Sang-Chan,Park, Soon-Dal,Byun, Joon-Seok 대한한의학방제학회 2001 大韓韓醫學方劑學會誌 Vol.9 No.1

Objectives : 'Pi Wei Lun' is the principal medical work of Li Dongyuan's writings, which contains most of his medical theories. There are sixty-three prescriptions in 'Pi Wei Lun', and fifty-two of them were originated from him. This study was planned to present assistance to those who apply Dongyuan's theory clinically. Methods : We investigated those fifty-two prescriptions literary, and studied them from a viewpoint of the principle and method of treatment. Results : Dongyuan's fifty-two prescriptions can be classified into eight groups from the viewpoint of the principles and methods of treatment which are composed of 'tonify the spleen and stomach & elevate the spleen Yang', 'elevate the spleen Yang & expel fire', 'elevate the spleen Yang & relieve dampness', 'promote blood flow to eliminate blood stasis', 'invigorate the spleen', 'replenish Ki & and dispel phlegm', 'purgation with drugs warm in nature', and 'the other' eight methods. Conclusions : From above, we concluded that Dongyuan considered 'the primordial energy of the spleen and stomach' as the most important when making up prescriptions, but, at the same time, he used diverse methods of treatment on the ground of differentiation of syndromes.

반하사심탕(半夏瀉心湯)이 Doxorubicin에 의해 유발(誘發)된 심장독성(心臟毒性) 및 임상증상(臨床症狀)에 미치는 영향(影響)

신민규,황희정,김상찬,변준석,Shin Min-Kyu,Hwang Hui-Jeung,Kim Sang-Chan,Byun Joon-Seok 대한한의학방제학회 2002 大韓韓醫學方劑學會誌 Vol.10 No.1

The effect of Banhasasim-tang extracts on the cardiac toxicity and general symptom induced by Doxorubicin administration(Three injection protocol) were monitored using male ICR mice. The changes of body weight, clinical signs, necropsy findings and organ weights of heart were observed. The results were as followed. 1. Decrease of body weight after Doxorubicin treatment were dose-dependently inhibited by Banhasasim-tang extracts. 2. The degrees of anorexia, ataxia and dehydration that were observed in Doxorubicin treatment group were dose-dependently inhibited by Banhasasim-tang extracts. 3. Increase of absolute and relative heart weight observed in Doxorubicin treatment group were dose-dependently inhibited by Banhasasim-tang extracts. In addition. the degrees of heart congestion and enlargement were significantly and dose-dependently decreased after Banhasasim-tang extracts dosing groups compared to that of Doxorubicin treatment group. In conclusion, the toxicity of Doxorubicin treatment(decrease of body weights, clinical signs such as anorexia, ataxia and dehydration, changes of organ weights of heart) was inhibited and/or prevented by Banhasasim-tang extracts. According to these results. it is considered that Banhasasim-tang has some preventive effect against to toxicity induced by Doxorubicin.

정천화담항기탕(定喘化痰降氣湯)이 ovalbumin으로 유발된 천식(喘息)에 미치는 영향(影響)

안태호,황희정,김대준,박지현,최해윤,김종대,An Tae-Ho,Hwang Hui-Jeung,Kim Dae-Jun,Park Ji-Hyeon,Choi Hae-Yun,Kim Jong-Dae 대한한의학방제학회 2000 大韓韓醫學方劑學會誌 Vol.8 No.1

Objectives: In order to evaluate the effect of jungcheonwhadamgangki-tang on Balb/c mouse of allergy-sensitive to bronchial asthma induced by ovalbumin. Methods: The changes of diameter lumen of trachea which was upper respiratory organ, weight and gross appearance of lung, histology of lung and trachea, numbers of inflammatory cells in the bronchoalveolar lavage fluld(BALF) were observed. Results : The results are obtained as follows. 1. The diameters of trachea lumen were significantly increased in Jungcheonwhadam gangki-tang treated group as compared with control group. 2. Inflammatory cells including neutrophil and eosinophil in BALF were significantly. in Jungcheonwhadamgangki-tang treated group as compared with control group. 3. Weight of lung and black spots, which resulted from infiltration of inflammatory were significantly decreased in Jungcheonwhadamgangki-tang treated group as compared with control group.4. Hypertrophy of mucous membrane of trachea and bronchus and bronchioles in the lung, peritracheal, peribronchus and peribronchiolar inflammatory cell infiltration, and mucoid exudate deposit in the lumen were significantly decreased in Jungcheonwhadamgangki-tang treated group as compared with control group. Conclusions : It is considered that Jungcheonwhadamgangki-tang has somewhat favorable effect on the bronchial asthma because the bronchial asthma specific series of abnormalities in respiratory system were decreased after oral administration of Jungcheonwhadamgangki-tang in this study. In future, it is needed that the toxicological and dosage specific study of Jungcheonwhadamgangki-tang to use against bronchial asthma with safe.

철겹핍성 빈혈 치험(治驗) 1례(例) 보고(報告)

이윤희,김종대,황희정,Lee, Yoon-Hee,Kim, Jong-Dea,Hwang, Hui-Jeung 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.4

We extended to find out the effect of serveral kinds of herbal medicion that administated to the patient suffer from iron deficiency anemia, that was prescribed according to differentiation of syndromes. Iron deficiency anemia is generlly differentiated as defiency of Ki and blood, hypofunction of the spleen and stomach, and insufficiency of the spleen and kidney in orinetal medicine. This patient was differentiated as defiency of Ki and blood. hypofunction of the spleen and stomach. So we prescribed Hyangsayangwi-tang, Ickibohyoul-tang and Ickibohyoul-tang ga Antler for the patient in turn. When administated Hyangsayangwi-tang and Ickibohyoul-tang, there was a little improvement in symptom but no improvement in hemonalysis. When administrated Ickibohyoul-tang ga Antler there was dominent improvement in both of symptom and hemonalysis. From this case. it is thought that added Antler-tonifying recipe is very effective to iron deficiency anemia and that more studies and examinations are needed in futher.

半夏瀉心湯이 Doxorubicin에 의해 誘發된 毒性에 미치는 影響

신민규(Min-Kyu Shin),황희정(Hui-Jeung Hwang),김상찬(Sang-Chan Kim),변준석(Joon-Seok Byun) 대한한의학회 2003 대한한의학회지 Vol.24 No.1

반하백출천마탕(半夏白朮天麻湯)이 Doxorubicin에 의해 유발(誘發)된 독성(毒性)에 미치는 영향(影響)

김봉석,오중환,임희용,백정한,박치상,김상찬,변준석,황희정,Kim Bong-Suk,Oh Jung-Han,Lim Hee-Yong,Beak Jung-Han,Park Chi-Sang,Kim Sang-Chan,Byun Joon-Seok,Hwang Hui-Jeung 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Object : The effect of Banhabakchulchunma-tang extracts on the hepatic, splenic and cardiac toxicity induced by Doxorubicin administration(Three injection protocol) were monitored using male ICR mice. Methods : The changes of body weight, clinical signs, necropsy findings and organ weights of liver, spleen and heart were observed with blood GOT and GPT levels. Results : 1. Decrease of body weight and The degrees of anorexia, ataxia and dehydration after Doxorubicin treatment were dose-dependently inhibited by Banhabakchulchunma-tang extracts. 2. Increase of absolute and relative liver and heart weight observed in Doxorubicin treatment group were dose-dependently inhibited by Banhabakchulchunma-tang extracts. In addition, the degrees of liver congestion necrotic spot and the degrees of heart congestion enlargement were dose-dependently decreased after Banhabakchulchunma-tang extracts dosing groups compared to that of doxorubicin treatment group. It is also demonstrated that elevated serum GOT and GPT levels in doxorubicin treatment group were significantly decreased in Banhabakchulchunma-tang extracts dosing groups. 3. Decrease of absolute and relative spleen weight observed in doxorubicin treatment group were dose-dependently inhibited by Banhabakchulchunma-tang extracts. In addition, the degrees of splenic atrophy were significantly and dose-dependently decreased after Banhabakchulchunma-tang extracts dosing groups compared to that of doxorubicin treatment group. Conclusion : the toxicity of doxorubicin treatment(decrease of body weights, clinical signs such as anorexia, ataxia and dehydration, changes of organ weights of liver, spleen and heart, elevation of serum GOT and GPT levels) was inhibited and/or prevented by Banhabakchulchunma-tang extracts. According to these results, it is considered that Banhabakchulchunma-tang has some preventive effect against to doxorubicin induced toxicity.

마미증후군 환자 증례보고(症例報告)

곽민아,이은주,박치상,변준석,박창국,황희정,Kwak, Min-Ah,Lee, Eun-Ju,Park, Chi-Sang,Byun, Joon-Seok,Park, Chang-Gook,Hwang, Hui-Jeung 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.4

Cauda Equina Syndrom is caused by compression of nerve root resulted from trauma or neoplasm and so forth in the lumbasacral lesion, so the patients suffer from the incontinence or urgency in urination and feces. We are extended to find out the effect of serveral kinds of Herbal Medicine that administrated to the patient suffering from Cauda Equina Syndrom. This patient was differenciated as defiency of Yang. When administrated Palmijihwang-Hwan gami-Bang, there was dominent improvement in both of symptom and dermatome test. From this case it is thought that Herbal Medicine treatment according to differenciation of symptom is very effective to Cauda Equina Syndrom and that more studies and examinations are needed in futher.