‘황희 정승 납거미 유언’ 설화 고찰

황인덕 ( In-deok Hwang ) 충남대학교 인문과학연구소 2018 인문학연구 Vol.57 No.1

알려져 있듯 황희는 정승을 지낸 조선 초기 명인의 한 사람이다. 다수일반인들에게 그는 주로 ‘황희 황정승’으로 이름과 직위가 병칭되어 불리어오곤 했는데, 이는 그의 이름이 오래 전에 이미 애칭화되어 후대까지 널리 불려왔다는 뜻이다. 우리나라 역대 정승을 거친 수많은 훌륭한 인물가운데, 그 직위에 요구되는 역할을 가장 충실하게 실현함으로써 그 존재가 거의 ‘정승’의 대명사처럼 기억되어온 결과라 하겠다. 황정승이 조선조전 시대를 대표하는 명 정승으로 알려져온 것에 부합하여, 그에 대하여 구전되어온 설화 또한 그 폭과 깊이가 넓고 깊게 실현되고 있다. 한 인물이 남긴 설화가 대략 주인공이 지닌 인품, 생애, 시대, 업적 등의 조건에 비례한다면, 황정승이 남긴 설화의 다양성도 대략 그러한 조건들이 예사인물과는 다른 결과라고 할 수 있다. 이 글은 위 인물에 대하여 <공작이도 납거미를 먹고 살랴>라는 하나의 유형을 집중적으로 고찰하였다. 주요 내용은 다음과 같다. 첫째, 위 설화의 유형은 크게는 하나, 작게는 세개의 유형으로 나뉘고 있다. 둘째, 위 설화가 형성된 화소의 원천은 ‘고려장 설화’와 ‘공작이도 납거미를 먹고 살랴’라는 전래 속담이며, 이 두 가지 요소가 하필 황정승을 대상 인물로 하여 새로운 설화를 이룬 것은 나말여초 중국과 조선의 국제관계와 그 속에서 황정승이 수행한 역할과 위상이 반영된 결과이다. 셋째, 설화를 통한 인물 형상의 특징은 청렴상을 통한 민담성의 강화에 있으며, 특히 비범한 능력을 발휘하여 청렴을 실천하는 인물상의 구현은 황정승의 투철한 애민정신을 반영한 결과라 할 수 있다. 넷째, 황정승 설화는 여말 선초를 지나 조선 초기 국운 상승기의 인물담을 대표적으로 보여주며, 그로 인하여 설화 속의 인물상이 한결같이 긍정적인 모습으로만 나타난다. As we all know, Hwang Hee was a renowned minister in the early period of Joseon dynasty. The fact that he has been mainly called both by name and position as ‘the minister Hwang’ means he was already given a nickname a long time ago. He has been remembered as a representative of ministers existed in the history as he carried out his duties the most faithfully among the numbers of past outstanding ministers in the country. In accordance with his reputation as a devoted minister throughout the whole dynasty of Joseon, his fictional image was embodied with great wide and depth. If the tales Hwang Hee left behind depended on the conditions such as his personality, his life, the period and his achievement, a variety of his tales would seem to approximately result from those different conditions from those of others. This paper concentrates on his tale < Apeacocklivesonflatspiders > only. The contents are as below. First, the above-mentioned tale might be broadly divided into a type, or into three types in detail. Second, a subject matter of this tale is derived from folk tales about ‘Goryeojang’ and ‘A peacock lives on flat spiders’. A new tale about Hwang Hee which has been invented reflects on the international relationship of Joseon with China, and the role and position he played in the late period of Goryeo dynasty and the early period of Joseon dynasty. Third, a characteristic of the hero in the tale depends on the strengthening his integrity, which displays Hwang Hee’s strong love for the people by showing his exceptional ability to perform for them. Fourth, the tale of Hwang Hee represents a tale of the great man in the history when the country was in the ascendant in the early period of Joseon from the late Goryeo and the early Joseon. Therefore, he was displayed as a positive character in the tales.

논문 : 무용공연에서 무용수의 미적 기능에 관한 연구

황인주 ( In Joo Hwang ) 한국무용기록학회 2012 무용역사기록학 Vol.27 No.-

무용공연에서 무용수의 창조적 영역이 존재함에 따라 무용작품이 지니고 있는 미적 특성과 가치에 대한 올바른 인식과 이해와 관련하여 무용공연에서 무용수가 수행하는 미적 기능이 무엇이며, 무용수의 창의적 역량을 개발하기 위해 고려되어야 할 요인들이 무엇인지에 대한 논의는 매우 중요하다. 따라서 본 논문에서는 이러한 중요성에 따라 안무가-무용수-관객이라는 구성요소를 중심으로 무용공연의 미적 의사전달체계와 특성을 살펴보고, 이를 토대로 무용공연에서 무용수가 수행하는 미적 기능을 분석하였다. 공연행위자로서 무용수를 통해 안무가의 안무메시지가 메시지전달자로서 무용수를 통해 관객에게 전달되고 전달된 무용표현에 대해 관객이 미적으로 반응하게 된다는 무용공연의 미적 전달체계는 비공연예술과는 달리 무용수라는 독특한 전달매개체의 존재로 인해 전달과정에서 안무가-무용수 간의 비평적 이해, 그리고 무용수-관객 간의 미적 상호작용이라는 두 가지 관계성이 발생된다. 이러한 미적 관계성에 따라 무용공연에서 무용수는 미적 가치의 전달, 미적 가치의 창조, 그리고 미적 표현의 구현이라는 상호 연계된 미적 기능을 수행하며, 이러한 기능들이 어떻게 수행되는가에 따라 무용공연의 예술적 성공이 결정된다. 무용공연에서 무용수가 수행하는 세 가지 기능은 무용수의 창조성을 요구한다는 점에서 무용수의 창조적 역량 개발에 대한 교육적 중요성은 매우 크다. 무용수의 창조성 개발을 위해서는 무용규칙 및 관례 등 무용테크닉 습득, 그리고 미적 소양 제고를 위한 교육이 중요하다. 특히 미적 개념과 특성에 대한 이해가 이러한 개념과 특성을 무용연습을 통해 무용표현으로 구현 함으로써 개발되고 증진된다는 점에서 미적 소양을 강화시키는 차원에서 진행되는 무용미학 교육은 그 자체가 독립적 분리되어 실시되기보다는 미적 표현을 위한 무용연습과 상호 결합되어 수행되는 것이 보다 바람직한 것으로 판단된다. As there exists the creative realm of the dancer in dance performance, it is necessary to analyze aesthetic functions of the dancer in dance performance and relevant elements related to the development of the dancer`s creativeness, in order to properly understand aesthetic properties and values in dance works. Therefore, the purpose of this study is to analyze the aesthetic communication model and its characteristics in terms of the choreographer, the dancer and the audience. Based on the aesthetic communication system, this paper also analyzes aesthetic functions performed by the dancer in dance performance. There are two relationships among elements in the aesthetic communication system, critical interpretation between the choreographer and the dancer, and aesthetic interaction between the dancer and the audience. Through these relationships, aesthetic properties are created beyond the choreographic content presented by the choreographer. Additionally, the dancer performs three interrelated functions such as delivering aesthetic values, creating aesthetic values, and embodying aesthetic expression, in the aesthetic communication system. Because these functions performed by the dance demand the dancer`s creativity, it is important to educate the dancer in order to develop the dancer`s creativity. In this sense, it is necessary to educate not only the dancer in terms of dance technique including dance rules and conventions, but also aesthetic knowledge. Because understanding aesthetic concepts and properties is enhanced through embodying dance expression in dance practices, the education in dance aesthetics may be needed to combine with dance practices for aesthetic expression.

‘참을 인자 세 번이면 살인도 면한다’ 설화 고찰

황인덕(Hwang, In-douk) 충남대학교 충청문화연구소 2020 충청문화연구 Vol.23 No.-

한국에는 ‘참을 인(忍)자 세 번이면 살인도 면한다’는 속담이 있다. 그리고 그에 대한 설화도 아주 널리 전승되고 있다. 이 설화의 줄거리는 며칠간 출타했다가 귀가한 남편이 아내가 외간 남자와 동침하는 것으로 오인하여 살인을 하려는 위기에서 ‘참을 인’자를 세 번 떠올리면서 화를 참아내 마침내 살인을 면할 수 있었다는 내용이다. 그런데 중국에서도 이와 흡사한 설화를 찾아볼 수 있다. 이 논문은 위 설화의 한국설화로서의 특징을 살피면서, 중국과의 비교적인 관점으로 연장해 보려는 것이다. 남편이 외출했다가 귀가했을 때, 집안에 있던 아내가 외간남자와 동침한 것으로 의심받을만한 상황은, 세계 어디에서나 일반적으로 실제이야기(real tale)로서 발생할 수 있는 일이다. 한국에서는 이 실담이 하나의 고정된 구전 설화 유형(traditional tale type)으로 정착되어 있고, 더불어 그것을 기반으로 한 ‘속담’도 널리 전승되고 있다. 이 설화는 중국에도 유사한 각편(version)이 전승되고 있음이 발견되는데, 그러나 이제까지 중국에서는 단 하나의 각편 자료만이 확인되고 있다 (이 글에서 인용한 자료는 22편인데 그러나 여기에 더 추가될 가능성이 있다). 위 설화는 과거 ‘야담’이라 불리던 조선시대 문헌설화(또는 기록설화)에는 보이지 않는다. 그러나 이것이 한국에서 전승되기 시작한 역사는 짧지 않을 것으로 보인다. 그리고 이러한 오랜 전승을 거치면서 위 설화는 독자적인 유형성이 다듬어지고, 동시에 한국설화다운 특징을 잘 갖추게 되었다고 할 수 있다. 여기에서 한국설화다운 특징은 사회 ‧ 문화 ‧ 이념, 그리고 근현대의 역사적 경험 등 여러 요인이 함께 작용한 결과라고 이해할 수 있다. 그렇다면 이 설화는 두 나라에서 독자적으로 발생한 것일까? 아니면 한국에서 발생하여 중국으로 전파된 것일까? 그러나 이 문제는 좀 더 나중에 판단을 해야 할 것으로 본다. In Korea, there is a proverb, "One moment of patience may ward off great disaster" And stories about this are also very widely handed down. The storyline tells us that after a few days of going out, a returning husband was able to endure anger and finally could avoid committing murder by thinking three times of "bearing" in a crisis of misunderstanding that his wife was guilty of sleeping with an outside man. Similar stories also can be found in China. This paper examines the characteristics of Korean folk tales and extends them to a comparative perspective with Chinese tale. When a husband returns home from outing, a situation in which his wife in the house might be suspected of sleeping with an outside man can generally be a real tale anywhere in the world. In Korea, this real story is settled as a fixed traditional tale type, and the "proverb" based on it is widely transmitted. Similar versions of narratives are found in China, but only one piece has been identified in China. (I used 22 pieces in this article, but I think there is a possibility to add more) This narrative tale is not found in the Chosun Dynasty literary narrative (or documentary narrative), which was formerly called Yadam. However, the history of this transmission in Korea is unlikely to be short. And through these long traditions of transmission, the tale has been refined its own typology, and at the same time, it can be said to have the features of Korean tale. Here, characteristics of Korean narrative tales can be understood as the result of various factors such as society, culture, ideology, and modern and contemporary historical experiences. So was this narrative tale generated in those two countries? Or did it originate in Korea and spread to China? However, I think this question could have an answer later.

UV조사에 의해 유도된 피부섬유아세포의 세포사에 미치는 Sea Buckthorn (Hippophae rhamnoides L.) 열매추출물의 영향

황인식(In Sik Hwang),고은경(Eun Kyoung Koh),김지은(Ji Eun Kim),이영주(Young Ju Lee),곽문화(Moon Hwa Kwak),고준(Jun Go),성지은(Ji Eun Sung),송성화(Sung Hwa Song),황대연(Dae Youn Hwang) 한국생명과학회 2014 생명과학회지 Vol.24 No.5

Sea buckthorn (Hippophae rhamnoides L.)은 flavonoid, carotenoids, steroids, vitamin, tannins, oleic acid 등의 생물학적으로 활성이 높은 성분을 풍부하게 함유하고 있어 오랫동안 피부질환(skin diseases), 위궤양(gastric ulcers), 천식(asthma), 폐질환 등에 사용되어 왔다. 본 연구에서는 아시아와 유럽의 고지대에서 야생으로 자라는 Sea buckthorn의 열매추출물(SBFE)이 UV조사 후 유도되는 세포사와 연관성을 규명하기 위하여, 세포사와 관련된 다양한 인자의 변화를 관찰하였다. 그 결과, 세포사를 유발할 수 있는 UV조사농도는 400 mJ로 설정하였고, 세포생존율은 UV + LoC, UV + MeC, UV + HiC 처리군에서는 유의적으로 증가하였다. 또한 FACS와 DAPI염색의 결과에서 PI로 염색된 세포수는 UV + vehicle 처리군에서 급격히 증가한 이후 UV + SBFE 처리군에서 유의적으로 감소하였고, Annexin V가 염색된 세포 수는 고농도의 SFBE을 처리한 UV + HiC 처리군에서 유의적으로 감소하였다. 그러나 PI와 Annexin V가 동시에 염색된 세포의 수는 UV + LoC 처리군에서 오히려 증가하였고 나머지 그룹에서는 유의적인 변화가 관찰되지 않았다. 한편, 세포사와 관련된 단백질의 변화에 대한 연구에서 caspase-3는 pro 형태와 active 형태 모두에서 유의적인 차이를 나타내지 않았으며, Bax와 Bcl-2 모두 UV 무처리군에 비하여 UV + Vehicle 처리군에서 감소하였으나 Bax의 발현양은 UV + SBFE 처리군에서 농도의존적으로 증가한 반면, Bcl-2의 발현양은 UV + SBFE 처리군에서 추가적으로 더욱 감소하였다. 따라서 이상의 결과들은 SBFE가 UV조사에 의해 유도된 세포사를 억제하거나 회복시키는 기능을 가지고 있음을 제시하고 있어 향후 UV조사에 의해 유발되는 다양한 상해에 대한 치료제로서의 가능성을 제시하고 있다. Sea buckthorn (Hippophae rhamnoides L.) is a well-known and rich source of biologically active compounds, such as flavonoids, carotenoids, steroids, vitamins, tannins, and oleic acid. The effects of sea buckthorn fruit extract (SBFE) on ultraviolet (UV)-induced cell death was investigated in SK-MEL-2 cells cotreated with UV and a low concentration (LoC), medium concentration (MeC), or high concentration (HiC) of SBFE. Cell viability gradually decreased in accordance with an increase in the UV dose. The cell viability of the UV+SBFE cotreated cells increased significantly compared to that of UV+vehicle-treated cells during the application of an appropriate UV radiation dose (400 mJ). In addition, the number of 4’,6-diamidino-2-phenylindole (DAPI), propidium iodine (PI)-, and annexin V-stained apoptotic cells was higher in the UV+vehicle-treated cells than in the UV untreated cells. The decrease of apoptotic cell numbers varied in each treated group, but it was most significant in the SBFE-treated group. The number of PI-stained cells dramatically decreased in accordance with the concentration of SBFE, and the maximum decrease was detected in the UV+HiC-treated group. In addition, Bax expression increased and Bcl-2 expression decreased in the SBFE-treated group compared with the UV-only treated group. The level of caspase-3 remained constant in all the groups. These results suggest that SBFE may contribute to a recovery from UV-induced cell death through the regulation of apoptotic protein expression and that it may have potential therapeutic utility in ameliorating UV-induced skin ageing.

도로변 완충녹지의 식재구조에 따른 초미세먼지(PM_2.5)농도 저감효과 연구 - 서울 송파구 완충녹지를 대상으로 -

황광일,한봉호,곽정인,박석철,Hwang, Kwang-Il,Han, Bong-Ho,Kwark, Jeong-In,Park, Seok-Cheol 한국조경학회 2018 韓國造景學會誌 Vol.46 No.4

본 연구의 목적은 도시계획시설로 조성되는 완충녹지가 $PM_{2.5}$(초미세먼지) 저감에 효과가 있는지를 규명하고, 도로변 완충녹지의 구조, 녹량(녹지용적), 식재유형에 따른 초미세먼지 변화량을 분석하여 초미세먼지 저감을 위한 완충녹지 조성 시 활용할 수 있는 요소들을 도출하는 것이었다. 연구대상지는 송파구를 선정하였고, 양재대로, 송파대로에 인접한 5개의 완충녹지에서 16개 조사구를 선정해 현장조사를 실시하였다. $PM_{2.5}$ 농도 측정결과, 조사구별 평균농도는 계절 상관없이 보도 $46.6{\mu}g/m^3$, 녹지 $45.5{\mu}g/m^3$, 주거지 $42.9{\mu}g/m^3$로 모두 도로($53.2{\mu}g/m^3$)보다 낮았으며, 주거지 농도 값이 가장 낮았다. 완충녹지의 농도 저감효과 확인을 위해 완충녹지의 녹량과 초미세먼지 상대비율간의 상관관계 분석결과, 교목과 관목 전체 녹량이 높은 그룹의 상대비율이 낮은 것으로 확인되어 완충녹지 녹량의 영향력을 증명했으며, 관목 부족형이 교목 부족형보다 농도상대비율이 더 높은 것으로 확인되어 관목의 영향이 더 큰 것으로 판단되었다. 교목과 관목의 식재구조에 따른 녹피율과 녹지용적계수가 복합적으로 $PM_{2.5}$ 농도 저감에 영향을 미치고 있었으며, 교목의 열수와 관목의 층위구조가 $PM_{2.5}$ 농도 저감에 중요한 영향요인으로 판단되었다. 특히, 초미세먼지 농도가 높은 겨울철 완충녹지의 $PM_{2.5}$ 농도저감 특성 분석결과, 관목의 녹피율이 중요한 요인이었다. This study aims to verify the effect of green buffers, built as urban planning facilities on the reduction of ultra-fine particulate($PM_{2.5}$) and analyze changes in ultra-fine particles by structure, green volume and planting types of wayside green buffers, thus drawing the factors that can be used when green buffers are built to reduce ultra-fine particulate based on the results. This study selected Songpa-gu, and investigated 16 sites on 5 green buffers adjacent to two of Songpa-gu's main roads, 'Yangjaedaero' and 'Songpadaero'. This study divided all the green spaces into three different types-slope type, plain type and mounding type, and analyzed the mean green volume. As a result of measuring the concentration of $PM_{2.5}$, this study found that it was $55.5{\mu}g/m^3$ on average in winter, which was a harmful level according to the integrated environmental index provided by Seoul City, saying that levels above $50{\mu}g/m^3$ may have a harmful effect on sensitive groups of people. Particularly, the concentration of $PM_{2.5}$ was $38.6{\mu}g/m^3$ on average in spring, which exceeded the mean concentration of $PM_{2.5}$ in Seoul City in 2015. The mean concentrations of $PM_{2.5}$ in every investigation spot were $46.6{\mu}g/m^3$ for sidewalks, $45.5{\mu}g/m^3$ for green spaces and $42.9{\mu}g/m^3$ for residential areas, all of which were lower than $53.2{\mu}g/m^3$ for roads, regardless of the season. The concentration of $PM_{2.5}$ for residential areas was the lowest. In the stage of confirming the effect of green buffers, this study analyzed the correlation between the green volume of vegetation and the fluctuated rate of ultra-fine particles. As a result, it was found that the green coverage rate of trees and shrubs was related to the crown volume in every investigation spot but were mutually and complexly affected by each other. Therefore, this study judged that the greater the number of layers of shrubs that are made, the more effective it is in reducing the concentration of $PM_{2.5}$. As for seasonal characteristics, this study analyzed the correlation between the concentration of $PM_{2.5}$ for residential areas in winter and the green coverage rate of each green space type. As a result, this study found that there was a negative correlation showing that the higher the shrub green coverage rate is, the lower the concentration value becomes in all the slope-type, plain-type and mounding-type green spaces. This study confirmed that the number of tree rows and the number of shrub layers have negative correlations with the fluctuated concentration rate of $PM_{2.5}$. Especially, it was judged that the shrub green volume has greater effect than any other factor, and each green space type shows a negative correlation with the shrub coverage rate in winter.

아계(鵝溪) 사행몽교탄생(使行夢交誕生) 담 연구

황인덕(Hwang In-deok) 충남대학교 충청문화연구소 2010 충청문화연구 Vol.5 No.-

Lee, San-hae [1538-1609} was the personality equipped with talented gifts, and he was a dignitary in charge of Yeonguijeong in the period of the Joseon Dynasty. There exist several legends of figures relevant to him which have been orally transmitted to these days. This writing is on the one of the legends. This story is about "Sahaengmonggyotansaeng" in which the father of Lee, San-hae was sent to China as an envoy, where he lay with his wife and begot a son in his dream. This story type has a lot more versions in comparison with other types related to Lee, San-hae. This explains why this legend has gained lots of attention from the public. The above mentioned legend dates back to Seong, Mong-jeong[1471-1517] ahead of Lee, San-hae. It could be the result of the figure story in the precedent period being pulled by a more famous figure in the following period. However, there exists a difference in these two stories. There still remains a vestige of mythical motif in Seongmongjeong story, which means that the Seongmongjeong story has been orally transmitted to this period while assuming strongly a meaning of "A Great Man who put his family in the map" in the socio-cultural background where the tradition of one's family was much valued. However, the Lee, San-hae Story has been able to be imagined and remembered easily to the general public by virtue of the level of awareness of a figure, and peculiarity of the figure's name-i.e. consistency of the name Lee, San-hae with the place name "Sanhae'gwan." By this, it could be concluded that Lee, Sanhae legend, though it is the same type story, has been orally transmitted to this period while taking on more intriguing quality and popularity. However, the more important difference lies in another. In short, in the legend by Lee, San-hae, there clearly appear the narrative elements such as 'Shadowlessness', 'Bonelessness' and 'Transformed into water as soon as he died.' This could be the result of the general public's consciousness of criticism against his political weakness being strongly reflected. His responsible role in history was big, so such criticism was strongly levelled at Lee, San-hae. This is a natural consequence.

체외에서 돼지 정자-난자의 상호작용시 투명대내 Lectin 결합

황인선,김정익,정희태,양부근,박춘근,Hwang, In-Sun,Kim, Choung-Ik,Cheong, Hee-Tae,Yang, Boo-Keun,Park, Choon-Keun 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.3

Objective: Lectins are cell-agglutinating and sugar specific proteins or glycoproteins of non-immune origin that precipitate glycoconjugates having saccharides of appropriate complementarity. Because of these properties, plant lectins have been used to help characterize the carbohydrate moieties of glycoproteins in the zona pellucida (ZP) of several mammalian species including pigs. Treatment of oocytes with various lectins blocks sperm binding to the ZP in various mammalian species. This study was undertaken to examine the distribution of sugar residues in the ZP of pig oocytes matured in vitro and the ability of spermatozoa to bind to ZP and in vitro penetration in oocytes treated with fluorescein isothiocyanate (FITC)-labelled lectins. Materials and Methods: The lectins of Banderiaea simplicifolia (BS-II, bind to $\beta$-D-N-acetylglucosamine), Canavalin ensiformis (Con A, bind to $\alpha$-D-Mannose), Lens culinaris (LCA, bind to a-D-Mannose), Ricinus communis (RCA-I, bind to $\beta$-D-Galactose) and Ulex europaeus (UEA-I, bind to $\alpha$-L-Fucose) were examined for spermatozoa penetration, binding capacity to ZP and distribution of lectins. Results: The penetration rates were significantry (p<0.05) higher in control oocytes (63%) than those treated with all lectins, but penetration rates ($40{\sim}49%$) were simililar in group treated with lectins. The incidence of monospermy was similar in oocytes untreated and UEA-I, but it was higher in oocytes treated with BS-II, Con A, RCA-I and LCA. The porcine oocytes cultured for 48 h in TC-199 medium were freed from cumulus cells and treated for 30 min with fluorescein isothiocyanate-labelled lectins. When examined under fluorescein illumination, higher (p<0.001) proportions of oocytes showed fluorescein of zona pellucida after treatment with Con A (93%), LCA (93%) and RCA-I (100%) than BS-II (37%) and UEA-I (50%). All of the oocytes treated with RCA-I exhibited strong fluorescein in the outer region of the zona pellucida while those treated with LCA exhibited strong fluorescein throughout the zona pellucida. BS-II bounded mainly to the outer region and UEA-I bounded mainly to the inner region of the zona pellucida, with either strong or weak fluorescein. At 120 min after insemination in vitro, fewer spermatozoa were bound to the zona pellucida of the oocytes treated with BS-II, Con-A and RCA-I. Of the lectins, Con A most inhibited sperm binding. Conclusions: These results suggest that $\beta$-D-Galactose residues in the porcine zona pellucida may act as primary sperm receptors and inducers of the sperm acrosome reaction and these sugar residues may be involved in the block to polyspermy.

흰쥐 콩팥 세관 상피 세포에서 E-cadherin의 발현

황인아(In-A Hwang),하헌주(Hunjoo Ha),한기환(Ki-Hwan Han) 대한해부학회 2008 Anatomy & Cell Biology Vol.41 No.1

E-cadherin은 상피세포의 이음복합체에 존재하는 주요접합분자이며, 상피세포의 극성을 유지하고 세포간 상호작용을 담당한다. E-cadherin은 콩팥에서 발현되는 것으로 알려져 있으나 세관에 따른 정확한 발현양상은 아직 알려져 있지 않다. 본 연구의 목적은 콩팥세관에 따른 E-cadherin의 발현양상을 관찰하는 것이다. Sprague-Dawley계 흰쥐의 콩팥을 분리하여 면역조직화학염색과 western blot을 실시하였다. 세관에 따른 E-cadherin의 분포를 관찰하기 위해 세관 상피세포의 지표인 bumetanide-sensitive Na+/K+/2Clcotransporter(BSC1), thiazide-sensitive Na+/Cl- cotranstporter(TSC), calbindinD28k, H+-ATPase과 함께 이중염색을 실시하였다. 흰쥐 콩팥에서 E-cadherin은 상피세포의 기저외측막에서 발현되었다. 집합관과 콩팥유두상피세포에서 강하게 발현되었고, 근위곱슬세관, 헨레고리, 헨레고리의 내림가는부분에서 약하게 발현되었다. 원위곱슬세관과 헨레고리의 굵은오름부분에서 중간 정도의 발현을 보였으며, 핵하부세포질에 퍼져있는 양상으로 발현되었다. 토리상피세포와 근위곧은세관에서 E-cadherin이 발현되지 않았다. E-cadherin의 단백정량결과 겉질과 바깥속질에 비해 속속질에서 E-cadherin이 많이 발현되었다. 이상의 결과로 E-cadherin은 콩팥세관 상피세포에 따라 다른 발현양상을 보이며, 집합관과 콩팥유두상피세포에서 주요 접합분자로 작용함을 알 수 있었다. E-cadherin is a cell adhesion molecule that is expressed abundantly in the kidney. However, the expression pattern in various renal epithelial cells is not well established. The purpose of this study was to determine the cellular localization along the nephron segments in the rat kidney. Kidneys from adult Sprague Dawley rats were fixed in 4% paraformaldehyde and processed for immunohistochemistry. Bumetanide-sensitive Na+/K+/2Cl- cotransporter (BSC1), thiazide-sensitive Na+/Cl- cotransporter (TSC), calbindin D28k, and H+-ATPase were used to identify the thick ascending, distal convoluted stubule, connecting tubule, and collecting duct, respectively. In the rat kidney, Ecadherin was expressed mainly in the basolateral domain of the collecting duct and papillary surface epithelial cells. The expression level of E-cadherin changed gradually in the connecting tubule and became moderate in the distal convoluted tubule, thick ascending limb, and loop of Henle. The S1 and S2 segment of the proximal tubule showed weak immunoreactivity. However, E-cadherin was not expressed in the S3 segment of the proximal tubule in the rat kidney. These results suggest that E-cadherin is a major adhesion molecule in the collecting duct and papillary surface epithelium, and that E-cadherin may play a critical role in maintaining the epithelial polarity of these nephron segments.

토양잔류 농약의 식물흡수에 대한 모델예측 및 관리전략

황정인 ( Jeong-in Hwang ),김장억 ( Jang-eok Kim ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

재배환경 중 작물에 살포된 농약은 직접 또는 간접적으로 토양에 떨어져 잔류할 수 있고, 잔류된 농약의 일부는 작물체로 흡수되어 잔류문제를 야기할 수 있다. 하지만 토양 중 잔류농약의 기준설정과 관리방안에 대한 연구는 여전히 미미한 실정이다. 본 연구에서는 토양잔류 농약의 식물흡수예측 모델식을 개발하고 예측된 결과를 이용하여 토양 중 잔류농약에 대한 관리기준을 제시하고자 하였다. 개발된 모델식의 적용성은 유기염소계 살충제 엔도설판의 α 및 β 이성질체를 대상으로 상추에 대한 흡수이행 실험을 실시하여 얻은 실험값을 모델식을 이용한 예측값과 통계적으로 비교함으로써 평가되었다. 그 결과, 개발된 모델식에 대한 적용성은 β 이성질체에 대해 39.2%의 편차를 보이며 양호하였으나 α 이성질체에 대해서는 176.3%의 상당한 편차를 보이며 낮았다. 또 한 예측된 결과로부터 제시된 상추재배 토양 중 엔도설판의 관리기준은 1.6 mg kg-1이었으나, 토양 중 생성되는 주요 대사체인 엔도설판 sulfate를 고려하여 더 낮은 농도에서 기준이 설정되어야 했다. 한편, 토양 중 잔류하는 엔도설판의 근원적인 제거를 위해 zero-valent iron (ZVI)과 ascorbic acid (AA)를 이용한 화학적인 토양복원기술도 개발하였다. 수중 분해시험을 통해 가장 효과적인 엔도설판 분해시스템(AA/ZVI/H2O2 시스템)을 조사하고 이 시스템을 토양에 적용한 결과, 엔도설판의 분해율은 α 이성질체에 대해 64.0%, β 이성질체에 대해 66.2%로 나타났으며 분해 반감기 또 한 2.7~2.8일으로 상당히 빨랐다. 전체적으로 이 연구에서 개발된 식물흡수예측 모델식과 화학적인 토양복원기술은 토양잔류 농약의 식물흡수에 대한 사전관리 방안으로 고려될 수 있었다. Most of pesticides deposited on soils either after direct or foliar spraying could be persisted in the soil, and a portion of their residues may result in the plant uptake via root. Although the plant uptake of pesticides from soil can contribute to safety problems for final agricultural products, there are no assessment tools and standards for managing the pesticide residues in soils. In this study, we developed a mathematical model for predicting theoretically the plant uptake of pesticide residues from soil and suggested a management standard for pesticide residue in soil based on the modeled results. An accuracy of the developed model was assessed by statistically comparing the modeled values with the values measured in the uptake experiments of α- and β-isomers of endosulfan (ED) by lettuce grown on ED-treated soils. As a result, the developed model was available for β-ED with the deviation of 39.2%, but not for α-ED with high deviation of 176.3%. Based on the modeled values, the management standard for ED in lettuce-cultivating soil was proposed at concentration of 1.6 mg kg-1. However, this standard concentration should be established lower than 1.6 mg kg-1 due to the consideration of toxic metabolite ED-sulfate produced with the degradation of isomers. On the other hand, we developed a chemical remediation technology to remove fundamentally the ED isomers from soil. Through aqueous degradation experiment, we found that the use of combined reagents of zero-valent iron (ZVI), ascorbic acid (AA), and H2O2 was the most effective system for the degradation of ED isomers in water, and this system was applied to remove the isomers in soil. By the use of AA/ZVI/H2O2 system, the degradation rates of α- and β-ED in soil were 64.0 and 66.2%, respectively, and their degradation half-lives were short at 2.7~2.8 d. In conclusion, overall results in this study suggest that technologies such as theoretical model prediction and chemical soil remediation could be used as advanced management strategies to prevent previously the undesirable plant uptake of pesticide residues from soil.

무용교육과정에서의 무용테크놀로지 및 매체 수용에 관한 연구

황인주 ( In Joo Hwang ) 대한무용학회 2009 대한무용학회논문집 Vol.61 No.-

In recent years, new dance technology and medium have been introduced in various dance areas. Considering that dance may expand its boundary and move into the another development stage by utilizing new dance technology and medium, it is important to accept new dance technology and medium in dance education, and let dance students try various dance experiments with these digital technologies and medium in order to get rich dance experiences. For these technologies and medium to be accepted and utilized effectively and efficiently in dance curriculum, it is necessary to develop an acceptance model to new dance technology and medium, and empirically test which critical factors influence acceptance decisions at the student level. Then, based on the the technology acceptance model, the purpose of this study is to find out not only determinants affecting user acceptance of new dance technology and medium in dance education, but also test the relationship among factors in the acceptance process. The main results of this study are as follows. First, based on previous studies on digital technology acceptance in the educational environment, the acceptance model of new dance technology and medium include social influence, shared trust, innovation, interest, self-efficacy as external variables. Secondly, while social influence, shared trust and innovation affect perceived usefulness, interest and self-efficacy affect perceived ease of use respectively. This implies that each external variable has different influence on user`s perception. Thirdly, while perceived usefulness and acceptance attitude positively influence dance students to accept new technology and medium, perceived ease of use does not directly influence the intention of acceptance. This suggest that perceived usefulness may play a key role in the acceptance model. The outcome of this study may be used as a reference to design several methods to increase students` acceptance of new dance technology and medium, and activate these technologies in the dance curriculum. However, considering that research on the acceptance model in the dance field is very limited but the subject is important, it is hoped that more research in this area be done in the near future.