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4.7T에서 생체내 자기공명분석법을 이용한 신생아 뇌에서의 인 자기 공명분석 소견
서대철,문치웅,이대근,김기수,이윤,임태환,박철민,최혜영,박형섭,황온유,유시준,Suh, Dae-Chul,Moon, Chi-Woong,Lee, Tae-Keun,Kim, Ki-Soo,Yi, Yun,Lim, Tae-Hwan,Park, Cheol-Min,Choi, Hye-Young,Park, Hyoung-Sup,Hwang, On-You,Yoo, Shi-Joon 대한영상의학회 1993 대한영상의학회지 Vol.29 No.1
In vivo 31P NMR spectra were obtained in eight infant brain at 4.7T. Each phosphorus metabolite and its ratio were analyzed to evaluate the brain damage and maturity, and compared with the reported data obtained at the lower field strength, Measurement of T1 relaxation time at 4.7T was done in an infant and a cat brain invivo. PCr/Pi and PCr/$\beta$-ATP ratio were used as a marker of brain damage. PME/PDE revealed higher values than those of the reported data obtained at the lower field strength and the defference was partly attributed to the long T1 relaxation time of PME rather than the brain immaturity. Although the resolution of the spectrum was improved at 4.7T, a long repetition time is recommended to minimize T1 difference of phosphorus metabolites of brain at 4.7T.
살리실산 나트륨이 Guinea Pig 내이 외림프의 Unknown Compoung에 미치는 영향
김상윤,황온유,김혜진,추광철 울산대학교 의과대학 1993 울산의대학술지 Vol.2 No.2
There are some evidences that temporary hearing loss is induced by disturbance of cochlear microcirculation due to the inhibition of prostaglandin synthesis by salicylate, however, the mechanism of salicylate ototoxicity has been still under study. We previously suggested that the increase of unknown compound concentration in perilymph of guinea pig might be important for the mechanism of salicylate ototoxicity. So we tried to compare the time course of unknown compound concentration to salicylate concentration in perilymph. Sodium salicylate(460mg/kg) was injected intraperitoneally to guinea pig. Unknown compound concentration in perilymph was measured before the injection and 1 hour, 3 hours, 5 hours, 7 hours and 24 hours after injection, using HPLC-ECD. The time course of unknown compound concentration reveals maximum level at 5 hours and nearly control level at 24 hours, and this is similar to the change of salicylate concentration in perilymph. These data might be another evidence to our understanding of unknown compound-related salicylate ototoxicity.
양성 및 음성 증상 정신분열병 환자에서 혈장 Dopamine-β-hydroxylase 활성도에 관한 연구
박인호,이철,황온유,한오수,김창윤 大韓神經精神醫學會 1993 신경정신의학 Vol.32 No.1
The authors investigated the relationship between plasma DBH activities and positive/negative symptoms of schizophrenia as proposed by Crow`s two syndrome hypothesis and also the changes in the plasma DBH activities during 6 weeks of antipsychotic treatment. The subjects were DSM-Ⅲ-R schizophrenic or provisional schzophreniform patients(N=25) who were drug free at least for 4 weeks. Plasma DBH activities were measured with clinical symptom assessment repeatedly (N=25) and during antipsychotic treatment(N=15). Clinical symptoms were assessed with Positive and Negative Syndrome Scale (PANSS) and plasma DBH activites were found to be lower significantly in negative symptom schizophrenics compared with normal controls. This result suggests that plasma DBH activity may be a useful biological marker to delineate negative symptom subgroup of schizophrenia. It also supports the hypothesis that negative symptom subgroup of schizophrenia may be associated with decreased noradrenergic neurotransmission. The plasma DBH activities decreased after 6 weeks of antipsychotic treatment in positive symptom subgroup of schizophrenia but not in negative symptom subgroup of schizophrenia. However the changes in plasma DBH activities after the antipsychotic treatment were not significantly different between positive and negative symptom subgroup. And also the changes in clinical symptoms did not correlate with the changes in the plasma DBH activities. Based on these findings, the changes in plasma DBH activities were speculated as not to reflect the changes in clinical symptoms but as the effects of antipsychotic drugs.
Purification and Determination of in vitro and in vivo Activities of Recombinant Human Annexin Ⅰ
Kim,Chong-Kook,Cho,Sung-Woo,Hwang,On-you,Lee,Jae-Dam,Song,Kyu-young,Kim,Kyoung-Mi,Lee,Jong-Wook,Na,Doe-Sun 울산대학교 의과대학 1993 울산의대학술지 Vol.2 No.1
아넥신 Ⅰ은 칼슘의존적으로 인지질막에 결합하는 단백질군의 하나인 아넥신류에 속하며 상피성 성장 인자 수용체 키나제의 기질로 알려져있다. 그러나, 생체내 역할과 그 기전에 대하여는 밝혀지지 않고 있다. 아넥신Ⅰ의 생물학적 기능에 대한 자세한 연구를 하기위해 재조합 아넥신Ⅰ을 수용성 상태로 생산하였고, 대장균에서부터 EGTA추출한 후, DE-52 이온 교환, gel filtration, hydroxylapatite 칼럼을 통과시켜 대량으로 정제하였다. 정제된 아넥신Ⅰ은 시험관내에서 PLA를 억제하였고, 생체내에서 쥐 발바닥 부종에 대한 소염 효과를 나타내었다.
백서 뇌에서 선택적 세로토닌 재흡수 차단제인 Sertraline이 Serotonin Transporter mRNA의 조절에 미치는 영향
김창윤,김성윤,홍진표,이철,황온유,한오수 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5
연구목적 : 항우울제인 sertraline이 전사 수준에서 serotonin transporter의 유전자 발현에 어떤 영향을 주는 지에 대해 상반된 결과가 보고되고 있어 이를 확인하고자 본 연구를 시행하였다. 방 법 : 각 군 당 백서 5마리를 대상으로 처치군에는 sertraline 10mg/kg을, 대조군에는 생리적 식염수를 복강내에 매일 일회 2주간 투여하였다. 마지막 약물 투여 후 24시간 뒤 고정액으로 관류하여 뇌를 적출한 뒤 raphe 부위를 냉동 절삭하여 얻은 절편들에 대해 미리 준비된 ³(???)S-dATP가 부착된 780bp serotonin transporter cDNA probe와 보합결합(in situ hybridization)을 시행하고, 슬라이드에 부착시킨 다음 X-선 필름에 노출시켜 나타난 serotonin transporter mRNA 신호를 image analyzer로 정량 분석하였다. 통계 자료 분석은 대응되는 절편에 대해 paired t-test를 적용하였다. 결 과 : Sertraline 투여 시 serotonin transporter mRNA 농도가 증가하는 경향을 보였다(p<0.05). 결 론 : 이러한 연구 결과는 sertraline 투여에 따라 시냅스 내 serotonin이 증가함에 따라 이를 보상하기 위해 감소시키는 방향으로 serotonin transporter의 유전자 발현이 조절되는 것으로 해석할 수 있다. The knowledge of gene regulation of serotonin transporter mRNA may provide clues to understanding how antidepressants affect their therapeutic actions. Recently, the effects of antidepressants on the serotonin transporter have been investigated but yielded controversial results. To study this further, we performed in situ hybridization for serotonin transporter mRNA in rats(treatment group, n=5)receiving long term(14 days) treatment with a selective serotonin reuptake inhibiting antidepressant, sertraline(10mg/kg, i.p). Following sertraline treatment, a significant(P<0.05)increase in hybridization of serotonin transporter mRNA was observed compared to that observed in vehicle-treated rats(control group, n=5). This result may be interpreted as a compensatory mechanism to reduce synaptic levels of serotonin which were increased by long term sertraline treatment.
사람 내피세포에서 염증 전구사이토카인이 VCAM - 1의 발현에 미치는 영향
조영주,박수길,이재담,황온유,문희범,장윤혜,김승후,홍혜남 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.2
Background: The expression of adhesion molecules contribute to development of systemic diseases. Vascular cell adhesion molecule-l(VCAM-1) is an endothelial cell membrane glycoprotein that has been implicated in leukocyte/endothelial cell interactions in inflammation. Ojective: The aim of this study was to characterize the surface expression and regulation of VCAM-1 on two different endothelial cells. Methods: We examined the effects of the expression of VCAM-1 in two different endothelial cells, isolated from human umbilical cords and human glomerulus. Expression of VCAM-1 was measured by enzyme-linked immunosorbent assay(ELISA) and flow cytometry. Results: In human umbilical cord endothelial cells(HUVECs), both interleukin-lβ(IL-lβ) and tumor necrosis factor-α(TNF-α) increased VCAM-1 expression. VCAM-1 expression increased by TNF-αwas higher than that increased by IL-lβ. In human glomerular endothelial cells(HGECs), IL-lβand TNF-αmarkedly increased VCAM-1 expression. Conclusion. The regulation of VCAM-1 appears to be somewhat different in HGECs compared with HUVECs. These differences between the responsiveness of the two cells may possibly indicate inherent differences in endothelial cell derived from different vascular beds.