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차대룡,김난희,최동섭,백세현,최경묵,김동림,한상엽 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.5
Background: Increased loss of proteoglycan (PG) characterized by an increased loss of anionic charges in the basement membrane has been considered as one of main factors causing urinary loss of albumin. The glycosaminoglycans (GAGs) are linear polymers of repeated disaccharides and the GAG chains are covalently bound to core proteins, forming proteoglycans. It is known that urinary N-acetyl-β -D-glucosaminidase (NAG) excretion is a sensitive marker of renal damage and is increased before other renal functional parameters. The aim of this study was to investigate whether GAG treatment is capable of influencing urinary protein and NAG excretion in Otsuka Long-Evans Tokushima Fatty (OLETF) rats which are known as type 2 diabetic animal model. Methods: Fifteen male OLETF rats and twenty male Long-Evans Tokushima Otsuka (LETO) rats were used for this study. LETO rats are non-diabetic control rats. All OLETF rats were randomly assigned to 2 groups: control group (n=10) given only . tap water and GAG group (n=5) feeding with GAG 10 mg/kg from 7 weeks to 55 weeks of age. Measurement of body weight, blood glucose, serum BUN and creatinine was performed periodically. 24-hour urine collection for measurement of urinary protein and NAG excretion was done at 17, 25, 37, 46, 55 weeks of age. Results: 1) OLETF rats showed higher body weight, blood glucose, 24-hour urinary protein and NAG excretion compared with LETO rats. But serum concentration of BUN and creatinine were not different between OLETF and LETO rats. 2) GAG-treated OLETF rats exhibited lower urinary protein/creatinine excretion (17.48±0.50 vs 22.49±0.11 mg/mg Cr, p $lt; 0.05) and NAG (17.40±5.94 vs 43.73±7.44 nmol/h/mg Cr, p $lt; 0.05) excretion compared with non-treated OLETF rats. But body weights, blood glucose, serum concentration of BUN and creatinine were not different between GAG-treated OLETF rats and non-treated OLETF rats. Conclusion: 1) The urinary excretion of NAG may be a possible early marker of diabetic nephropathy in OLETF rats. 2) Urinary protein and NAG excretion were decreased in the GAG-treated OLETF rats. GAG seems to have a protective effect against development of diabetic nephropathy.
배양된 메산지움세포에서 당수송체-1 발현에 대한 Protein Kinase C 억제제의 효과
차대룡,김신곤,김난희,최동섭,백세현,최경묵,신동현,박이병,김동림 대한당뇨병학회 2001 Diabetes and Metabolism Journal Vol.25 No.3
Background: Recent studies have suggested that increased glucose uptake via GLUT1 may be a major determinant of glucose utilization and extracellular matrix formation in mesangial cells. This study was to evaluate the effect of protein kinase C inhibitor on glucose transporter-1 (GLUTI) expression in cultured rat mesangial cells. Methods: The GLUT1 expression was evaluated in mesangial cells exposed to various glucose concentrations of media (5.5 mM, 15 mM or 30 mM) and incubation times (6 hr, 24 hr or 72 hr) by semiquantitative RT-PCR and western blot analysis. The effect of protein kinase C (PKC) inhibitor, calphostin C and phorbol 12-myristate 13-acetate (PMA) on GLUT1 expression was also evaluated under the same conditions. Results: The GLUTI mRNA expressions were significantly increased in MG (15 mM) and HG (30 mM) than those in NG (5.5 mM) with incubation of 6 hr, 24 hr and 72 hr, respectively. In HG media, the GLUT1 mRNA expression with incubation of 24 hr and 72 hr were significantly increased than that with incubation of 6 hr, respectively. In HG media, the GLUTI mRNA expressions were significantly reduced in calphostin C and PMA treated groups compared with those in untreated groups. In western blot analysis of HG media, GLUT1 proteins were identified in PMA- or calphostin C-untreated group and PMA b hr treated group, but not identified in PMA 24 hr treated group and in calphostin C-treated groups with incubation of 6 hr and 24 hr. Conclusion: PKC inhibitors decrease glucose-induced GLUT1 expression under high glucose concentration in mesanglal cells. These results suggest that PKC pathway may regulate GLUT1 expression under high glucose concentration in cultured rat mesangial cells.
혈액투석 개수 후 단핵구배양 상청액에 의한 사람 근위 세뇨관 상피세포의 Fas 유전자 발현에 대한 연구
차대룡(Dae Ryong Cha),조원용(Won Yong Cho),윤종우(Jong Woo Yoon),조상경(Sang Kyung Jo),김형규(Hyung Kyu Kim),장경현(Kyung Hyun Chang) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.3
N/A Residual renal function rapidly declines after the initiation of hemodialysis and its mechanisms are supposed to be associated with frequent hypotensive episodes during hemodialysis and subsequent ischemic injury to remnant nephron, but blood-membrane interaction might play an important role because of its ability to activate complement system and other various humoral and cellular mechanisms. Blood monocytes are activated by complements, bacterial conta- minants and activated monocytes are known to secrete multiple proinflammatory cytokines such as TNF-α, IL-1β. The expression of TNF-αand IL-1β in mRNA and protein level were examined by RT-PCR and ELISA respectively in patients with ESRD after the initiation of hemodialysis. Author also investigated the mRNA expression of Fas in human proximal tubular cell culture in the presence of TNF-a and PBMC culture supernatant before and after the ini-tiation of hemodialysis. Compared to PBMC separated before the initiation of HD, the amount of cytokine mRNA from PBMC separated after the initiation of HD showed increased tendency from 0.97±0.2 to 1.12±0.28 for TNF-α(p=0.29), from 1.03±0.18 to 1.10±027 for IL-1β (p=0.54). TNF-αand IL-lβ protein level in PBMC culture supernatant also showed increased tendency from 2.25±0.5 to 4.254±3.77 for TNF- α(p=0.10), from 3.5±2.08 to 4.0±4.3 for IL-1β(p=0,25). TNF-a in-creased Fas mRNA expression dose-dependently com-pared to control but it was not stat.istically significant(p=0,37, 0.22). Compared to the the level of Fas expression in HPTC cultured in the presence of pre HD PBMC supernatant, the level of Fas expression increased significantly in the presence of post HD PBMC supernatant(0.64±057 vs 1.05±0.12, p=0.01). As a conclusion, cytokine gene expression and secretion can increase as a result of blood-membrane interaction and these might have some influence on the loss of residual renal function in CRF patients maintained on hemodialysis.
차대룡(Dae-Ryong Cha),성진현(jin-Hyuen Seong) 산업기술교육훈련학회 2009 산업기술연구논문지 (JITR) Vol.14 No.3
Today, the goal-oriented machine tool industry, precision, high-speed, complex function, and are collaborating with a robot. A machine tool itself automatically to replace the cutting tool 5-axis machining center at a time, and ends this operation. Therefore, to improve the structure of the Muti-articulated robot can enhance the strength, productivity is expected to have a big effect. Structural Analysis and Dynamic characteristic was able to improve our weak joints. Awards and to improve the bottom two places about 22% Dynamic characteristic be improved. Therefore, the optimal design of the robot working space and high-rigidity and if you can take advantage of the flexibility in the development of a good industrial robot.