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조윤상(Yoon Sang Cho),남보우(Bo Woo Nam),홍사영(Sa Young Hong),김진하(Jin Ha Kim),김현조(Hyun Jo Kim) 한국해양공학회 2014 韓國海洋工學會誌 Vol.28 No.6
A numerical method to investigate the non-linear motion characteristics of a TLP is established. A time domain simulation that includes the memory effect using the convolution integral is used to consider the transient effect of TLP motion. The hydrodynamic coefficients and wave force are calculated using a potential flow model based on the HOBEM(higher order boundary element method). The viscous drag force acting on the platform and tendons is also considered by using Morison’s drag. The results of the present numerical method are compared with experimental data. The focus is the nonlinear effect due to the viscous drag force on the TLP motion. The ringing, springing, and drift motion are due to the drag force based on Morison’s formula.
강원도 벼 주산지에서 생태형별 이앙시기가 현미 품질에 미치는 영향
조윤상 ( Youn-sang Cho ),이지우 ( Ji-woo Lee ),윤예지 ( Ye-ji Yoon ),김용복 ( Yong-bok Kim ),정정수 ( Jung-su Jung ) 강원대학교 농업생명과학연구원 2022 강원 농업생명환경연구 Vol.34 No.0
강원도의 벼 주산지인 중부평야지(춘천), 동해안지(강릉), 중북부평야지(철원)에서 시험기간 중 출수 후 40일간 평균기온 기준 최적 등숙적온은 중부평야지 23.7°C, 동해안지 21.8°C, 중북부평야지 22.4°C였다. 중부평야지에서 벼생태형에 따른 품질은 조기보다 만기이앙이 좋았으나, 중북부평야지 및 동해안지의 중만생종 품종은 등숙기 적산온도가 부족하여 현미 품질이 급격히 저하되었다. 동해안지에서 종생종은 5월 20일 이앙시 품질이 가장 높았고, 중만생종은 6월 10일 이앙은 미숙립 등 품질이 낮아 안전재배에 적당하지 않았다. 중북부평야지에서 조생종은 5월 10일에서 5월 30일까지 이앙시 품질의 차이는 크지 않았으나 만기 이앙인 6월 10일 이앙에서 현미 품질이 급격히 저하되었다. 특히 중만생종은 현미 품질이 매우 낮아 재배는 권장할 수 없었다. 최근 기후변화나 이상기후 발생 증가에 따른 벼주산지에서 최적 품종 선발 및 이앙시기 재설정으로 재배안정성과 농가소득 향상에 기여할 수 있을 것으로 판단된다. Recent changes in the climate of Korea show that the average rate of temperature increase is above 1.5°C, which is more than twice the global average temperature increase rate of 0.74°C, indicating that the rate of warming here is faster than that in other regions. These rapid climate changes demand a response strategy that include the development of adaptive varieties and cultivation techniques in agricultural ecosystems. The major rice producing regions of Gangwon-do are diverse and located in the central plains, northern plains, and east coast. To obtain the basic data necessary for resetting the cultivation methods to adapt to climate change, the yield quantity and yield components of rice were analyzed based on different ecological types and their transplanting period. In the central plain, the yield of early and middle maturing varieties increased with the increase in average temperature. The yields were not stable for the high-quality cultivation of mid-late varieties. However, the temperature rise did not affect the yields of the early, middle, and mid-late maturing varieties in the east coast region. In the central and northern plains, cultivation stability was confirmed only for the early varieties. Therefore, cultivation of the middle and mid-late varieties is not recommended for high-quality rice production and maintaining cultivation-stability. In the climate change scenario, quality and stability of cultivation are the priority factors that must be considered in rice cultivation areas.
난황면역제를 이용한 개 주요 소화기 및 호흡기질병 방제에 관한 연구 II. 난황면역제의 실험동물 및 개에 있어서의 질병방제 효과
이희수,김종만,우승룡,정병열,조윤상,유한상,윤용덕,허원,문영식,오진식,Lee, Hee-Soo,Kim, Jong-man,Woo, Seung-ryong,Jeong, Byeong-yeal,Cho, Yun-Sang,Yoo, Han-sang,Yoon, Yong-dhuk,Huh, Won,Mun, Young-sik,Oh, Jin-sik 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3
Immunoprophylatic effect of IgY against B. bronchispetica was proven with 100% preventive rate in mice administrated with IgY with antibody titer 1:640~1:2,560. Intramuscular administration was more efficient than oral administration. This phenomenon was also observed in the therapeutic effects of IgY after challenge with B. bronchseptica in mice. In the field trials with the egg yolk antibodies from hens immunized with combined antigens with B. bronchiseptica and parvovirus, curing rates in dogs with severe clnical signs such as bloody diarrhea were 81.6% and 86.7% by intramuscular or subcutaneous administration of IgY, respectively. Safety of the antibodies in dogs was proven without any side effects such as vomiting, edema, fever, etc. by adminstration of double doses for 7 days. These results indicated that the egg yolk antibodies could be used as effective prevention and treatment of alimentary and respiratory diseases in dogs.
난황면역제를 이용한 개 주요 소화기 및 호흡기질병 방제에 관한 연구 I. 개 보데텔라, 파보바이러스 및 개디스템퍼 항원의 닭에서의 면역반응
이희수,김종만,우승룡,정병열,조윤상,탁동섭,임숙경,유한상,윤용덕,허원,문영식,오진식,Lee, Hee-soo,Kim, Jong-man,Woo, Seng-ryong,Jung, Byeong-yeal,Cho, Yun-Sang,Tark, Dong-seob,Lim, Sook-kyoung,Yoo, Han sang,Yoon, Yong-dhuk,Huh, Woo,Mun, Y 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1
This study was carried out to produce IgY against B. bronchiseptica, parvovirus and distemper virus that are major pathogens in alimentary and/or respiratory diseases of dogs. In the comparison of adjuvants, ISA70 was the best in the rapid induction and maintence of antibody titers. Agglutination antibody titers against B. bronchiseptica were 1:1,280 ~ 1:10,240 in sera and 1:160 ~ 1:1,280 in egg yolk. Hemagglutination inhibition(HI) titers against parvovirus in sera and egg yolk were 1:80 ~ 1:320 and 1:64 ~ 1:256, respectively. Virus neutralization titers against canine distemper was 1:8 ~ 1:64 in sera and egg yolk. These results suggested that egg yolk antibody titers could be variable according to a sort of adjuvant and antigens of the pathogens.
Mycobacterium bovis에 의한 소 및 사슴 결핵의 폐 병리조직학적 소견 비교연구
진영화,노인순,이경현,이경우,조윤상,주이석,Jean, Young Hwa,Roh, In Soon,Lee, Kyung Hyun,Lee, Kyung Woo,Cho, Yoon Sang,Joo, Yi Seok 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.2
Comparative studies of histopathologic lesions from 23 purified protein derivative (PPD) positive cattle, 21 slaughter cattle found with tuberculosis, and 11 tuberculosis-positive elk (Cervus elaphus) were performed. PPD positive cattle did not show specific histopathologic lesions in all 23 heads that were no visible lesion reactor. Slaughter cattle found with tuberculosis revealed microscopically classical granulomatous lesion (tubercle) with central caseous necrosis surrounded by mantle of epithelioid cells and Langhan's giant cells capsuled by connective tissue in lung. Elk was noted with some different lesion patterns with classical granulomatous lesion and suppurative abscesses that was composed of fibrin, degenerated cells without having connective tissue. In addition, many Langhan's giant cells infiltration in alveoli at peripheral lesion were observed in some cases of classical granulomatous lesion and suppurative abscesses.
Development of a multiplex-PCR for the rapid detection of Escherichia coli O157:H7 from raw beef
정석찬,정병열,윤장원,조윤상,김종염,박용호,Jung, Suk-chan,Jung, Byeong-yeal,Yoon, Jang-won,Cho, Yun-sang,Kim, Jong-yeom,Park, Yong-ho The Korean Society of Veterinary Science 1998 大韓獸醫學會誌 Vol.38 No.1
Esherichia coli O157 : H7의 slt I, slt II, uid A 및 eaeA 4종 유전자를 동시에 검출하기 위한 multiplex PCR 기법을 확립하고 쇠고기중 직접 E coli O157 : H7 검출시험을 실시하였다. 4 set의 primers를 이용한 multiplex PCR 기법으로 31종의 장내세균에 대한 특이성을 조사한 결과 E coli O157 : H7 에서 1,087bp (eae A), 584bp (slt II), 348bp (slt I) 또는 252bp (uid A)크기의 DNA를 동시에 특이적으로 검출할 수 있었다. E coli O157 : H7 15주는 모두 uid A 및 eae A 유전자가 동시에 검출되었고, 다른 장내세균에서는 검출되지 않았다. slt I 또는 slt II 유전자를 가지고 있는 E coli 표준균주 24종을 이용하여 multiplex PCR 기법과 Vero cell cytotoxicity assay을 비교검사한 결과 베로톡신 산생능과 PCR법의 결과는 일치하였다. mutiplex PCR 기법의 쇠고기중 검출한계는 modified EC(mEC)에서 증균없이는 E coli O157 : H7균 $10^4cells/g$ 이상에서 검출이 가능하였으나 mEC에 1차 증균후 modified TSB 증균하였을 경우에는 10cells/g이하까지도 검출이 가능하였다. 개발된 multiplex PCR 기법을 쇠고기 40종에 직접 적용한 결과 E coli O157 : H7은 검출되지 않았으나 slt I 및 slt II유전자를 가지고 있는 E coli 4종이 검출되었으며, 이들의 혈청형은 O6, O112, O115 및 O139 였다. 이 연구에서 개발된 multiplex PCR은 쇠고기중 E coli O157 : H7을 신속하고 특이적으로 검출하는데 사용할 수 있을 것으로 사료된다.