대뇌 피질 신경세포 일차 배양에서 리튬의 FeCl2에 의한Free Radical Injury 증강 기전 연구

이승혜,이영애,변한열,곽병주,정영기,노재성 대한정신약물학회 2006 대한정신약물학회지 Vol.17 No.5

Objectives: For the past half century, lithium has been used for the acute and prophylactic treatment of bipolar disorder and recurrent depression. Recently, new pharmacological effects of Li+ have appeared, showing that Li+ can influence neuronal injury. We tested the effects of Li+ on free radical induced neuronal injury in primary murine cortical cell cultures. Methods: Cortical cells were prepared from fetal mice (embryonic day 15) and exposed to 30μM Fe2+ alone or with 5mM Li+ or 5mM Li+ alone for 24 hrs at Days in vitro (DIV) 14. Neuronal death was analyzed by measuring lactate dehydrogenase (LDH) release into media. The fluorescence of 2',7'-dichlorofluorescin (DCF) was measured in as a mean of estimating the formation of reactive oxygen species (ROS). Results: Li+ alone does not produce neuronal injury itself but it potentiates Fe2+-induced neuronal injury through increasing the production of free radical. Conclusion: This study suggests that the effects of Li+ on neuronal survivorship may be injury type dependent and Li+ potentiate the free radical injury. Therefore in practice clinician should be cautious in using the lithium in the treatment of brain injured patients. 목 적 :리튬은 조울증 및 반복적 우울증의 급성기 치료 및 유지기의 재발 방지를 위해 가장 많이 쓰이는 약물이다. 이러한 리튬의 기분 안정에 대한 연구들 뿐만 아니라 신경 세포 보호에 대한 연구들이 늘어나고 있다. 하지만 리튬이 신경 세포사의 형태에 따라 특이적으로 작용하여 세포 자멸사(apoptosis)는 억제하지만 괴사(necrosis)를 더 증강 시킨다는 연구가 보고 되고 있어 본 연구에서는 리튬이 괴사(necrosis)를 더 증강시킨다는 가설을 세웠고 그 기전에 대해 알아보고자 하였다. 방 법 :임신 15일 된 횐 쥐를 사용하여 14일 간 배양된 대뇌 피질 세포에 24시간동안 Fe2+30 μM, Li+5 mM을 각각 단독처리하고 Fe2+30 μM에 Li+5 mM를 처리하였다. 신경 세포사는 죽은 세포로부터 배지로 분비되는 lactate dehydrogenase(LDH)의 활성을 측정하여 분석하였다. 세포내 fre radicals은 reactive oxy gen species(ROS)에 의해 산화된 dichlorodihydrofluorescein diacetate(DCDHF-DA)의 fluorescent signal를 통해 측정하였다. 결 과 :리튬 자체로 fre radical을 형성하여 신경 세포사를 유발하지는 않지만 Fe2+에 의해 유도된 free radical의 생산을 증가시킴으로써 괴사를 더욱 증강시킴을 알 수 있었다.

Unearthing the Past from the Ground: Buddhist Temples during the Goryeo Dynasty Seen in a New Light

이승혜 한국학중앙연구원 한국학중앙연구원 2019 THE REVIEW OF KOREAN STUDIES Vol.22 No.1

도시주변 농지감소지역의 공간 및 사회적 특성- 경상남도 김해시를 중심으로 -

이승혜,이유직 한국농촌계획학회 2018 농촌계획 Vol.24 No.1

This study was conducted for the purpose of deriving implications by observing the changing patterns and characteristics of the farmland reduction area in urban vicinity with Gimhae city, Gyeongsangnam-do as the subject. In order to achieve this goal, we first examined the problems and possibilities of farmland reduction area in urban vicinity through a theoretical review. Additionally, the characteristics of land use and community were examined for Gimhae city, Gyeongsangnam-do. The results of the study are summarized as follows. First, for 35 years from 1981 to 2015, Gimhae decreased 50.52㎢ of farmland, which is about 17.4 times that of Yoido, and about 69.4% of the decreased farmland area. Second, the decrease in agricultural land has been expanding to the whole of Gimhae City from 1990 to 2010, and has been continuing since 2010 around dong-area. Third, in the farmland reduction area in urban vicinity, the number of settlements increases rapidly, but the aging population also increases. Fourth, the composition of the community is getting complicated with the change of the members. Taken together, it is necessary to manage the area efficiently because rapid change is present in the farmland reduction area in urban vicinity. Based on this, the implications are summarized as follows. First, there is a change in land use due to the reduction of farmland not designated as agricultural development region. Therefore, it is necessary to supplement the farmland-conversion standard. Second, despite the fact that land use management has been carried out, there have been problems such as uncontrolled development due to the development pressure beyond institutional management, and therefore it is necessary to improve the structural defects of the pertinent legal system. Fourth, while the traditional farming activities are decreasing with the decrease of agricultural land area, the increase in farms with secondary jobs and the urban-rural interchanges organization’s efforts can lead to increased visits from outsiders that seek rural tourism and experiential learning.

高麗의 吳越板 『寶篋印經』 수용과 의미

이승혜 불교학연구회 2015 불교학연구 Vol.43 No.-

The Chongjisa edition of the Baoqieyin Dhāraṇī Sūtra 寶篋印陀羅尼經 (Kr. Bohyeobin gyeong), published in 1007, has received considerable scholarly attention as the oldest extant woodblock printed scripture with a frontispiece. The textual and material features of the Chongjisa edition of the dhāraṇī sūtra have been the subject of many previous studies. Scholars have also pointed out that the Chongjisa edition of the dhāraṇī sūtra bears close affinities with the three Wuyue editions of the same text, each published in the year 956, 965, and 975 under the patronage of Qian Hongchu. Qian Hongchu 錢弘俶 (r. 947-978) was the last king of Wuyue 吳越 (907-978), a kingdom that prospered in the southeast coastal region of China during the political chaos of the Five Dynasties and Ten Kingdoms period. However, little scholarly attention has been paid to the fact that the frontispiece of the Chongjisa edition of the dhāraṇī sūtra is closest to the first Wuyue edition of the dhāraṇī sūtra in terms of visual composition and iconographical motifs. What do these visual affinities between the Chongjisa and the first Wuyue edition indicate in light of the circulation and reception of the Baoqieyin Dhāraṇī Sūtra in East Asia? The primary objectives of this paper are twofold. First of all, I examine the publication and circulation of the three Wuyue editions of the dhāraṇī sūtra against the political and historical landscape of 10th century China and beyond. Secondly, I delve into the issue of Goryeo court’s reception of Wuyue court’s diplomatic gifts, namely the first Wuyue edition of the dhāraṇī sūtra (d. 956) and the bronze miniature stūpa, by taking the political realities of the two countries' courts into consideration. To this end, I first closely compare the frontispieces of the three Wuyue and Chongjisa edition of the dhāraṇī sūtra, and then proceed to analyze relevant textual and archaeological evidence in their historical context. Through this analysis, I showcase that only the first Wuyue edition of the dhāraṇī sūtra was transmitted to Goryeo, and was served as a model for the patron and carver of the Chongjisa edition. The Chongjisa edition of the dhāraṇī sūtra is, I argue, a historical product of an East Asia of the 10th-11th centuries characterized foremost by multi-state proliferation. 1007년에 개성 총지사에서 간행된 『보협인경(寶篋印經)』은 변상도가 있는 현존 최고의 목판인쇄경전으로 학계의 많은 관심을 받았다. 선행연구를 통해 총지사본의 형태와 서지학적 특징, 인쇄사적 의의가 밝혀졌다. 또한 총지사판 『보협인경』과 오대십국시대 중국의 강남지역에서 번성했던 오월(吳越, 907-978)의 마지막 왕 전홍숙(錢弘俶, 재위 947-978)이 956년, 965년, 975년 세 차례에 걸쳐 간행했던 『보협인경』과의 밀접한 관련성도 익히 지적됐다. 반면 1007년에 간행된 총지사본 변상도의 도상이 3종의 오월본 중 956년에 간행된 판본과 유사하다는 점은 간과됐다. 그렇다면 동아시아에서 『보협인경』의 유통과 수용이란 문제에 있어서 양자 간의 유사성이 의미하는 바는 과연 무엇일까? 이 논문의 주요 목적은 두 가지이다. 첫째로, 오월판 『보협인경』의 간행과 유통을 10세기 동아시아라는 역사적 상황에서 논의하는 것이다. 둘째로, 고려의 오월판 『보협인경』 수용을 경전의 공여자인 오월의 입장과 수용자인 고려 조정의 입장을 고려하여 살펴보는 데 있다. 이를 위해 이 논문에서는 먼저 3종의 오월본과 총지사본 변상도를 종합적으로 비교하겠다. 나아가 현재까지 알려진 문헌사료와 고고학적으로 발견된 유물들을 종합적으로 분석할 것이다. 이를 통해 956년에 간행된 오월본만이 고려에 전래되어 수용됐을 가능성을 논증하고, 그 원인을 오월을 둘러싼 10세기 중국의 역사적 상황과 당시 고려의 내부적 상황에서 찾아보겠다. 마지막으로 총지사판 『보협인경』이 다원적 천하관으로 대표되는 10-11세기의 동아시아 국제질서를 반영한 역사적 산물임을 밝혀 보겠다.

복장(腹藏)과 한국 불교미술사 연구 - 회고와 전망 -

이승혜 성신여자대학교 인문과학연구소 2020 人文科學硏究 Vol.41 No.-

복장(腹藏)은 불상, 불화의 조성을 마친 다음 불전에 봉안하기 전에 예경의 대상으로서 이들을 전화(轉化)하기 위해 거행되는 불교 의례와 의례 도중 납입된 모든 물목을 일컫는다. 복장은 늦어도 12 13세기에는 성립됐으며, 14세기에는 중요한 의례 중 하나로 자리 잡은 것으로 추정된다. 복장은 핵심 용기와 그 내외부의 장엄 요소를 중심으로 구성되나, 발원문을 비롯한 여러 문서, 불교전적과 사경, 다라니와 만다라, 직물과 복식 등을 포괄한다. 이와 같은 복장의 복합적인 성격상 서지학, 미술사, 불교사, 복식사 등 다양한 분야에서 그 개별적인 측면에 대해 부단히 연구해왔다. 그중 미술사학계에서는 크게 두 가지 측면에서 복장 자료를 불교미술사 연구에 활용해왔다. 첫째, 복장기록의 분석을 통해 불상의 제작배경, 제작자와 후원자의 성격, 불교 신앙의 양상을 분석했다. 둘째, 복장 유물과 소의경전의 비교를 통해 복장의 성립과 의례로서의 성격 규정을 시도했다. 특히 최신 연구에서는 복장의 밀교적 성격에 대해 집중적인 논의가 벌어졌다. 한국에는 밀교와 관련된 유물과 문헌이 영성하기 때문에 역사적으로 밀교가 뿌리내리지 못했던 것으로 이해돼왔다. 즉, 복장은 이와 같은 기존 자료의 한계를 보완하는 새로운 자료로서 고려시대 불교미술사 연구의 지평 확대에 이바지했다. 한편 복장의 밀교적 요소에 주목한 최근 미술사학계의 접근은 고려시대 밀교에 대한 역사학계의 기존 성과와 유리되어 진행된 측면이 있다. 학제의 벽을 넘어선 복장 자료의 통섭적인 연구를 통해 고려시대 불교미술사를 재조명할 필요가 제기된다. Bokjang (pokchang) refers to both a consecration ritual of Buddhist images and material remains of the ritual retrieved from the inner recesses of Korean Buddhist images. Bokjang deposit centered on a container functioning as the symbolic heart of the Buddha, but typically included votive texts, Buddhist texts and illuminated sutras, dharanis and mandalas, textiles and used clothing, etc. Due to the inclusive nature of the bokjang, scholars of diverse fields such as art history, bibliography, Buddhist studies and textile studies have examined individual aspects of the bokjang. In particular, art historians have utilized bokjang materials in two major ways. First, they examined issues of patronage, Buddhist beliefs, and historical background of the production of Buddhist images by examining votive texts and inscriptions found among the bokjang materials. Second, they attempted to define the nature of bokjang as a ritual based on comparisons of objects retrieved from the bokjang and esoteric Buddhist scriptures. In recent years, the bokjang materials received special attention for its potential value as primary sources that could supplement the paucity of textual sources on the esoteric Buddhist tradition in Korea. However, these new findings need to be reconsidered in light of the history of esoteric Buddhism during the Goryeo and vice versa.

대학생들의 사회적스타일 유형에 따른 도서관 이용특성 연구

이승혜,한정원 한국실내디자인학회 2019 한국실내디자인학회논문집 Vol.28 No.3

The role of the academic library is changing due to various social factors, and it is necessary to understand the changing needs of library users for efficient use of spaces. This study aims to recognize the necessity of user analysis in the academic library, and to find out the differences in the specific use of spaces by users’ social styles. Social Style Model was developed to study human behaviors using the specific patterns in social activities. With the scale of assertiveness and responsiveness, four kinds of social styles are defined; analytical style, driving style, expressive style, and amiable style. For this research, firstly, literature review was conducted to investigate the changing trend of the academic libraries, user characteristics, and Social Style Model. Secondly, spatial composition of the subject library was studied, and structured questionnaire was formed with the references of previous studies. Thirdly, questionnaire survey was executed on library users, and the data was analyzed by basic statistics, Chi-square test, and one-way ANOVA in SPSS 25. The result shows that there were differences in space use patterns, preferences, and satisfaction according to the different social styles. Analytical style showed a high rate of use in private study area and preferred quiet and open atmosphere to focus on learning. Driving style used group study area frequently and showed relatively high satisfaction rate. Expressive style showed high rate of use and satisfaction in Learning Commons, and preferred open atmosphere. Amiable style used group study area frequently and satisfied with the Learning Commons. This could be used to enhance the efficiency of space planning in academic libraries, and to construct guidelines for applying user characteristics and spatial preferences in library design.

Romiplostim-related Myelofibrosis in Primary Immune Thrombocytopenia: A Case Report

이승혜,박성우,강정훈,이원섭,김현영,송하나 대한내과학회 2018 대한내과학회 추계학술발표논문집 Vol.2018 No.1

또래 상호작용에서 나타나는 쌍둥이 유아의 관계적 특성: 언어적ㆍ비언어적 표현을 중심으로

이승혜,박선희 한국어린이문학교육학회 2018 어린이문학교육연구 Vol.19 No.4

본 연구는 쌍둥이의 교육에 필요한 학문적, 실용적 도움을 제공하는 것을 목적으로 또래 상호작용의 언어적ㆍ비언어적 표현에서 나타나는 쌍둥이의 관계적 특성 및 쌍둥이 출생순서에 따라 나타나는 관계적 특성에 대해 S시에 소재한 병설유치원에서 세 쌍의 쌍둥이 유아들과 학부모 그리고 교사를 연구 참여자로 하여 총 10개월에 걸쳐 연구되었다. 연구결과, 쌍둥이의 언어적ㆍ비언어적 표현에서 나타나는 관계적 특성은 배타적 결속 관계, 서로에 대한 정서적 유대감, 쌍둥이의 경쟁관계 등 세 가지 양상으로 나타났으며, 출생순서에 따라 첫째의 책임감, 양보, 권리의 특성, 둘째의 애교와 사랑스러움의 특성, 쌍둥이 간 형제 호칭에 의한 위계와 동료의식이 혼재된 관계적 특성을 보이는 것으로 나타났다. 본 연구는 쌍둥이가 긍정적 사회관계를 형성하고 쌍둥이라는 특수한 형제관계에 대한 이해를 통해 쌍둥이 양육과 교육에 교사와 부모의 협력적 역할 수행이 필요하다는 점을 시사한다. This study had started to observe twin children through teaching-learning process of twins in the childhood education field and analyzed the relational characteristics of twin children in peer interactions based on their verbal and non-verbal expression. The purpose of this study was to help understanding twin children's general language expression and to offer academic and practical assistance to parents who raise twins as well as to teachers. The study participants were three pairs of twin children who enrolled in a 4-year-old class at the Y public kindergarten located in Seoul, their parents, and 4 teachers who taught them. The study was done with participant observation, interviews, documents and audiovisual material as well as the researcher's journal for ten months from March 7 to December 23, 2016. The results of the study were as follows: first, the aspect of language expression in peer interaction between twin children showed three characteristics. They were the exclusive relational bond, emotional expression to each other, and competitive companion. Second, the twin children showed different aspects of language expression according to their birth order. First born twin in the peer interaction expressed a sense of responsibility, give-up, and right, while second born twin expressed the language of charming and loveliness. Twin children used hierarchical order title and this caused confusion among other peers. In a Korean kindergarten class, twin children called their siblings by his/her title given according to the hierarchical order rather than calling by his/her name like in western countries. The results of this study suggest that the understanding of twin children in peer interactions provides children and teachers to extend their learning environments and use various life situations as a hidden curriculum.

기계학습 기반 강 구조물 지진응답 예측기법

이승혜,이재홍,Lee, Seunghye,Lee, Jaehong 한국공간구조학회 2024 한국공간구조학회지 Vol.24 No.2

In this paper, machine learning models were applied to predict the seismic response of steel frame structures. Both geometric and material nonlinearities were considered in the structural analysis, and nonlinear inelastic dynamic analysis was performed. The ground acceleration response of the El Centro earthquake was applied to obtain the displacement of the top floor, which was used as the dataset for the machine learning methods. Learning was performed using two methods: Decision Tree and Random Forest, and their efficiency was demonstrated through application to 2-story and 6-story 3-D steel frame structure examples.

하이브리드 메타휴리스틱 기법을 사용한 트러스 위상 최적화

이승혜,이재홍,Lee, Seunghye,Lee, Jaehong 한국공간구조학회 2021 한국공간구조학회지 Vol.21 No.2

This paper describes an adaptive hybrid evolutionary firefly algorithm for a topology optimization of truss structures. The truss topology optimization problems begins with a ground structure which is composed of all possible nodes and members. The optimization process aims to find the optimum layout of the truss members. The hybrid metaheuristics are then used to minimize the objective functions subjected to static or dynamic constraints. Several numerical examples are examined for the validity of the present method. The performance results are compared with those of other metaheuristic algorithms.