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Qualitative and Quantitative Analysis for Microbiome Data Matching between Objects
유희상,옥연정,이송희,이소립,이영주,이민호,현성희,You, Hee Sang,Ok, Yeon Jeong,Lee, Song Hee,Lee, So Lip,Lee, Young Ju,Lee, Min Ho,Hyun, Sung Hee Korean Society for Clinical Laboratory Science 2020 대한임상검사과학회지(KJCLS) Vol.52 No.3
미생물 연구에서 대량의 마이크로바이옴 데이터를 효율적으로 얻는 기술이 발전해왔지만, 마이크로바이옴 빅 데이터를 적절하게 분석하는 도구는 여전히 부족하다. 또한 빈약한 데이터베이스를 사용하여 미생물 군집을 분석하면 잘못된 결과를 초래할 수 있다. 따라서 본 연구는 대량의 미생물 데이터베이스 분석을 위한 적절한 방법을 설계하고자 하였다. 박테리아는 개인의 손끝과 개인 소지품(휴대 전화 및 랩탑 키보드)에서 수집되었다. 박테리아로부터 게놈 DNA를 추출하고 16S rRNA 유전자를 표적으로 하여 차세대 시퀀싱을 실시하였다. 손끝과 개인 소지품 간의 박테리아 매칭 비율의 정확성은 공식과 함께 환경 및 인간관련 데이터베이스를 사용하여 확인하였다. 적절한 분석을 설계하기 위해 다음 세가지 범주를 기준으로: 정성적 분석과 정량적 분석 비교, 성별에 관계없이 모든 참여자뿐만 아니라 동일 성별 참여자 내 비교, 환경(eDB) 및 인간 관련 데이터 베이스(hDB)를 이용하여 샘플간 비교하였다. 결과는 정성적 분석과 동일 성별 참가자 내에서의 비교 및 hDB의 사용이 비교적 정확한 결과를 제공하였다. 우리의 연구는 인간 유래 미생물을 사용하여 대량의 미생물학적 데이터를 포함하는 연구를 수행할 때 정확한 결과를 얻을 수 있는 분석 방법을 제공한다. Although technological advances have allowed the efficient collection of large amounts of microbiome data for microbiological studies, proper analysis tools for such big data are still lacking. Additionally, analyses of microbial communities using poor databases can lead to misleading results. Hence, this study aimed to design an appropriate method for the analysis of big microbial databases. Bacteria were collected from the fingertips and personal belongings (mobile phones and laptop keyboards) of individuals. The genomic DNA was extracted from these bacteria and subjected to next-generation sequencing by targeting the 16S rRNA gene. The accuracy of the bacterial matching percentage between the fingertips and personal belongings was verified using a formula and an environment-related and human-related database. To design appropriate analysis, the bacterial matching accuracy was calculated based on the following three categories: comparison between qualitative and quantitative analysis, comparisons within same-gender participants as well as all participants regardless of gender, and comparison between the use of a human-related bacterial database (hDB) and environment-related bacterial database (eDB). The results showed that qualitative analysis, comparisons within same-gender participants, and the use of hDB provided relatively accurate results. This study provides an analytical method to obtain accurate results when conducting studies involving big microbiological data using human-derived microorganisms.
이태원,이동우,최연정,옥강민,박광용 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-
A novel tetrakis(4-(5,5-dimethyl-2-phenyl-1,3-dioxan-2- yl)phenyl)germane (2) was synthesized by the reaction of germanium(IV) tetrachloride with 4-(5,5-dimethyl-2-phenyl-1,3-dioxan-2-yl)phenyllithium. Colorless plate-shaped single crystals obtained from recrystallization in ether were characterized by the single-crystal X-ray diffraction. The organogermanium compound was crystallized in a noncentrosymmetric nonpolar tetragonal space group, I-4, and classified as a porous molecular material owing to the presence of two types of distinct channels as a result of intermolecular hydrogen bonding. Compound 2 exhibits a differential gas adsorption property. The powder second-harmonic generating (SHG) measurements indicate that compound 2 shows a SHG efficiency 10 times greater than those of α-SiO2 and type-1 nonphase-matchable.
오버레이 멀티캐스트 기반의 확장성 있는 VoD 서비스 모델
이형옥 ( Hyung-ok Lee ),손승철 ( Seung-chul Son ),곽용완 ( Yong-wan Kwag ),하연정 ( Youn-jung Ha ),남지승 ( Ji-seung Nam ) 한국정보처리학회 2007 한국정보처리학회 학술대회논문집 Vol.14 No.1
인터넷 상에서 VoD 서비스를 제공하기 위해서는 시스템 자원과 네트워크 대역폭을 효율적으로 사용해야 한다. 이러한 효율성을 높이기 위해서 오버레이 멀티캐스트를 생각 할 수 있지만 실제로 On-Demand 서비스에 적용하기 위해서는 많은 결점의 보완이 필요하다. 본 논문에서는 이러한 결점을 보완하고 기존 시스템의 간단한 확장으로 VoD 서버의 자원과 대역폭을 효율적으로 사용할 수 있는 오버레이 멀티캐스트 모델을 제안 한다. 제안된 모델은 호스트의 공유 버퍼와 패칭 채널 그리고 트리구성 알고리즘을 이용하여 사용자 데이터의 요구시점은 물론 다양한 재생율 지원 등 제한이 없는 On-Demand 서비스 수행이 가능하다.
기본간호학 실습교육방법에 따른 학생들의 간호수행능력의 비교
유문숙,유일영,박연옥,손연정 한국간호과학회 2002 Journal of Korean Academy of Nursing Vol.9 No.2
-Comparison of Student's Clinical Competency in Different Instructional Methods for Fundamentals of Nursing Practicum Purpose: The main purpose of this study was to compare the clinical competency in different instructio nal methods for funda- mentals of nursing practicum, standardized patients methods story as text method ,and traditional lecture/model method. Method: The study was designed as a quasi-experimental, nonequivalent control group post-test design with three separate classes of sophomore students attending fundamentals of nursing classes at one baccaleureate nursing school located in metropoli tan Seoul area. Control group was taught by traditiona lecture/ model method and two experimental groups were taught by standardized patients method and story as text method. Data were collected from September, 1999 to June 2001. There were 36 students in the standardized patient method group, 38 students in story as text group, and 40 students in the control group. Data analysis was done using SPSS WINDOW 9.0. Result: The results showed that the standardized patients method and story as text method groups were significantly better in clinical judgement and communication skills than the traditional lecture/model method group. The standardized patients method group was significantly better in clinical nursing skills performance than two other groups. However, there was no significant difference among the three groups in student satisfaction. Conclusion: The standardized patients method is an effective in teaching clinical cometency for student nurses. It is necessary to explore more efficient way to develop standardized patients cases for wider areas of nursing education. Also, it is recommended to develop more research projects with many nursing programs.