녹조의 생물학적 제어

안치용 ( Chi-yong Ahn ),오희목 ( Hee-mock Oh ),고소라 ( So-ra Ko ),르반베 ( Ve Van Le ),강민경 ( Mingyeong Kang ) 한국물환경학회 2023 공동춘계 학술발표회 논문집 Vol.2023 No.0

RFID 시스템 이용한 열차 위치검지용 빔폭 가변형 RFID 리더안테나

안치형(Chi-Hyung Ahn),조봉관(Bong-Kwan Cho),류상환(Sang-Hwan Ryu),오순수(Soon-Soo Oh) 한국철도학회 2015 한국철도학회논문집 Vol.18 No.2

N: P ratio 조절에 의한 미세조류 생장과 경쟁 제어

안치용 ( Chi Yong Ahn ),이재연 ( Jae Yon Lee ),오희목 ( Hee Mock Oh ) 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.2

Microalgae can grow autotrophically with the supply of light, carbon dioxide and inorganic nutrients in water through photosynthesis. Generally, microalgal growth is limited by the concentrations and relative ratio of nitrogen (N) and phosphorus (P) among the nutrients in the aquatic environment. Each microalga has its specific optimum N : P ratio resulting in dominance in a particular water having similar nutrient composition. Algal bloom is an immense growth of certain microalga commonly cyanobacterium and can be sequestrated by reducing the limiting nutrient, generally P in the freshwater. Moreover, dominance of a less toxic blooming strain can be established by manipulating N : P ratio in the water. On the other hand, microalgal biomass of a certain species can be enhanced by increasing limiting nutrient and adjusting the N : P ratio to the target species. The above-mentioned eco-physiological features of microalgae can be more completely interpreted in connection with their genomic informations. Consequently, microalgal growth regulation which can be achieved on the basis of its eco-physiological and further genomic insights would be helpful not only in the control of algal bloom, but also for an increased yield of algal biomass.

인제한에 따른 Scenedesmus quadricauda의 광합성 특성 및 질소, 이 함량 변화

안치용,김희식,윤병대,오희목,Ahn, Chi-Yong,Kim, Hee-Sik,Yoon, Byung-Dae,Oh, Hee-Mock 한국조류학회(藻類) 2002 ALGAE Vol.17 No.2

Photosynthetic parameters of Scendesmus quadricauda, such as the maximum photosynthetic rate ($P_{max}$), photosynthetic efficiency (α) and the initial saturation intensity of irradiance for photosynthesis ($I_K$) were obtained using photosynthesis-irradiance (P-I) curve in a phosphorus-limited chemostat. S. quadricauda exhibitied no photoinhibition until at 200 μmol·$m^{-2}$ . $P_{max}$ (r=0.963, P=0.002) and $I_K$(r=0.904, P=0.013) showed linear relationships with growth rate. Chlorophyll-α concentration and cell dry weight decreased at higher growth rates, ut chlorophyll-α content per cell dry weight increased. The increase in photosynthetic rates at higher growth rates was due to the increase of $P_{max}$ and $I_K$ which was caused mainly by the increase in the absolute amount of chlorophyll-α rather than the increased photosynthetic efficiency of individual chlorphyll-α. The α did not show a significant relationship with growth rate (r=0.714, P=0.111). The cell quota of carbon (r=0.554, P=0.254) was not correlated with growth rate, but cell quota of nitrogen (r=0.818, P=0.047) and phosphorus (r=0.855, P=0.030) exhibited linear correlations with growth rate.

DGGE를 이용한 대청호 수화 발생시기의 세균군집 분석

고소라,박성주,안치용,최애란,이정숙,김희식,윤병대,오희목,Ko So-Ra,Park Seong-Joo,Ahn Chi-Yong,Choi Aeran,Lee Jung-Sook,Kim Hee-Sik,Yoon Byung-Dae,Oh Hee-Mock 한국미생물학회 2004 미생물학회지 Vol.40 No.3

대청호에서 수화 발생시기인 2003년 7월에서 10월까지 분자생태학적 방법의 하나인 DGGE (denaturing gradient gel electrophoresis)를 이용하여 시간에 따른 세균군집구조의 변화를 연구하였다. 조사기간 동안 출현한 식물플랑크톤을 형태학적으로 분류한 결과 cyanobacteria, 규조류 및 녹조류가 발견되었고, 이 중 Microcystis, Chroococcus, Oscillatoria, Phormidium 속이 크게 우점하였다. 16S rDNA의 DGGE pronto 분석에 의하여 Microcystis flos-aquae와 Oscillatoria spp.가 우점하는 것으로 확인되었으며, Aphanizomenon flos-aquae는 8월 중순에 우점하는 것으로 확인되었다. DGGE profile을 토대로 cluster analysis를 적용하여 다양한 미생물 군집의 유사성을 비교한 결과, 9월 2일의 미생물 군집이 다른 시기의 시료와 확연히 다른 그룹으로 구분되었다. 결과적으로 분자 생물학적 방법은 형태적 분석방법과 유사한 결과를 보였으며, 일부 세균의 분포 및 변화, 미생물 군집의 유사성 등에 대한 추가적 정보를 제공하였다. The change of bacterial communities during cyanobacterial bloom was analyzed by DGGE in Daechung Reservoir from July to October in 2003. The traditional morphological analysis showed that the genera of Microcystis, Chroococcus, Oscillatoria, and Phormidium were dominated. The most frequent band in the DGGE profile by 16S rDNA sequence analysis was identified as Microcystis flos-aquae and the cyanobacterial bloom was peaked on September 2. Oscillatoria spp. were also identified and Aphanizomenon flos-aquae dominated in the middle of August. Judging from the analysis of the digitalized DGGE profiles using the cluster analysis technique, the microbial community on September 2 was considerably different from others. Consequently, it seems that the gene fingerprinting method can give not only the similar results to the traditional morphological method but also additional information on the bacterial species and similarity among the examined microbial communities.

cpcBA-Intergenic Spacer Region을 이용한 Cyanobacteria의 다양성 분석

최강국,박용하,안치용,배명숙,오희목,Choi Gang-Guk,Park Yong-Ha,Ahn Chi-Yong,Bae Myoung-Sook,Oh Hee-Mock 한국미생물학회 2005 미생물학회지 Vol.41 No.4

The cyanobacterial diversity was analyzed by restriction fragment length polymorphism (RFLP) of PCR-amplified rpcBA-Intergenic Spacer (IGS) genes and cpcBA-IGS gene sequencing with a sample collected at Chuso-ri in Daechung Reservoir on March 15, 2005, The Shannon-Weiner diversity index was 0.65, indicating that the cyanobacterial community structure was simple. PCR-RFLP profiles obtained were Phormidium spp. (58 clones), Anabaena spp. (14 clones), Microcystis spp. (4 clones), Spirulina sp. (1 clone) and uncultured cyanobacteria (2 clones). The PCR-RFLP of cpcBA-IGS revealed that Phormidium spp. and Anabaena spp. dominated in the invested sample. As a consequence, it seems that the analysis of functional genes such as cpcBA-IGS can be used for the species identification and community analysis of cyanobacteria. 대청호에서 수화 발생이 빈번한 추소리 수역에서 2005년 3월 15일에 채취한 시료를 대상으로 유전자 분식에 의한 cyanobacteria의 다양성을 조사하였다. rpcBA-Intergenic Spacer (IGS)는 cyanobacteria에 특징적 색소인 phycocyanin을 합성하는 유전자와 유전자 사이의 부분으로, 환경시료에서 cyanobacteria의 다양성을 조사하기에 매우 유용한 기능 유전자이다. cpcBA-IGS를 이용하여 restriction fragment length polymorphism (RELP)으로 cyanobacteria의 다양성을 분석한 결과 Phomidium 속은 58 clones, Anabaena 속은 14 clones, Microcyxtis 속은 4 clones, Spirulina 속은 1 clone 그리고 uncultured cyanobacteria 2 clones가 존재하였다. 전반적으로 Phormidium 속이 우점하였으며, 여름철에 수화를 일으키는 Anabaena 속과 Microcystis 속도 많이 분포하였다. 따라서 cyanobacteria는 cpcBA-IGS와 같은 기능 유전자에 의한 종 동정 및 군집분석이 가능함을 보였다.

Screening of Bioactive Materials from Freshwater Microalgae

이완석,최애란,안치용,오현철,안종석,오희목,Lee, Wan-Seok,Choi, Ae-Ran,Ahn, Chi-Yong,Oh, Hyun-Cheol,Ahn, Jong-Seog,Oh, Hee-Mock The Korean Society of Phycology 2004 ALGAE Vol.19 No.3

One hundred and fifty four micro algal strains, newly isolated from nationwide freshwaters in Korea, were screened for their anticancer, ant diabetic, and antibiotic activities. The micro algal strains were cultured with different nutritional conditions that were divided into 4 groups as follows; a normal Allen medium, nitrogen (N)-limited medium, phosphorus (P)-limited medium, and N and P-limited medium. Algal biomass was extracted with a mixture of acetone:H₂O (1:1, v:v) and the extracts were used for the screening of bioactive materials. Anticancer, ant diabetic, and antibiotic materials were screened by the methods of vaccinia Hl-related protein tyrosine phosphates (VHR DS-PTPase) inhibition, protein tyrosine phosphates 1B (PTP1B) inhibition, and paper disk. The inhibition activity of VHR DS-PTPase was observed in 18 strains, having a maximum 79% inhibition from Anabaena affinis and the inhibition activity of PTP1B was observed in 9 strains, having a maximum 97% from Sphaerocystis schroeteri. Microcystis aeruginosa incubated in an N and P-limited medium showed antibiotic activity in 8 species out of 13 pathogenic bacteria. As a whole, it seemed that the stressed condition such as N and/or P limitation increased the production of bioactive materials in micro algae.

소아 흉부 X-ray 검사 시 Exposure field 조정에 따른 유효선량의 평가

안준오(June-Oh Ahn):정지상(Ji-Sang Jung):김성식(Sung-Sik Kim):안치복(Chi-Bok An),박순규(Sun-Kyu Park),임재식(Jae-Sik Lim) 대한영상의학기술학회 2016 대한영상의학기술학회 논문지 Vol.2016 No.1

목 적 : 소아 흉부 X-ray 검사 시, DR system에서의 적절한 Exposure field 조정을 통해 유효 Exposure Index범위 내의 영상의 유효선량과 각 장기에서의 유효선량 및 cancer risk 확률을 비교함으로써, field size 조정에 따른 유효선량 및 장기 유효선량을 평가하기 위함에 있다. 대상 및 방법 : 본원의 디지털 진단용 엑스선 촬영장치인 Discovery XR656(GE Healthcare, Korea)을 사용하여, 소아 흉부팬텀을 이용한 Chest AP검사를 수행하였다. IEC 61257-1에서 권고하는 General Digital Radiography의 선질 RQA3 조건에 맞춰 SID는 110cm, 노출조건은 관전압 50kVp로 고정하고, mAs range는 위의 장비에서 적용할 수 있는 범위 안에서 0.5,0.63,0.8,1.0,1.25,1.6,2.0,2.5,3.2mAs으로 변화를 주며 exposure하였다. 위의 조건에 따라, Exposure field size를 20cmx20cm, 30cmx30cm, 40x40cm, 총 3그룹으로 분류하였다. 각 조건에서 총 243번의 exposure를 한 뒤, 이의 평균값을 구하였다. 또한 획득 된 영상들을 Exposure Index를 기준으로 세 그룹으로 분류하고, 이 중에서 유효 Exposure Index범위 내의 영상들만을 분류하여 DAP를 계산하고 a PC-Based Monte Carlo Program 2.0(PCXMC)를 사용, 유효선량을 도출하였다. 위에서 얻은 유효선량을 기준으로 DR system에서 소아 Chest AP(PED)검사 시, Exposure field size에 따른 유효선량 경향을 분석하였다. 또한 각 조건에서 선량계로 측정한 실측 선량 값을 측정하여 Phantom에서 흡수한 실제 선량 값을 비교하였다. 더불어, PCXMC2.0을 사용하여 각 장기에서의 유효선량과 cancer risk 확률도 계산하였다. 결 과 : 적정 노출 군(DI: -3.0이상, 2.0이하)에 포함된 영상 중에서 동일한 mAs를 기준으로 Field size가 증가할 때의 유효선량은 field size가 늘어남에 따라 유효선량도 증가하는 경향을 보였다. 반면 선량계를 통해 측정한 phantom에서의 실측 선량 값에서는 큰 변화가 없었다. 또한 PCXMC2.0을 이용한 각 장기에서의 유효선량 및 cancer risk 확률은 증가하는 경항을 보였다. 결 론 : 각 조건으로부터 획득된 영상의 Exposure Index 값을 기준으로 분류한 영상들의 결과 값을 통해 소아 Chest AP(PED)검사 시, 의료 방사선 종사자의 적절한 Field size 조정을 통해 소아 환자의 피폭 저감화 및 방사선 방어에 일조할 수 있다고 사료된다. Purpose : Pediatric chest radiography, DR system at the effective dose of the proper image within the range Exposure Index Exposure field through the appropriate adjustments by comparing the effective dose and cancer risk probability in each organ, It is in order to assess the effective dose and organ dose of the active field size adjustment. Materials and Methods : Using the present system of digital diagnostic X-rays taken of Discovery XR656 (GE Healthcare, Korea), Chest AP tests were performed using a pediatric chest phantom. SID is 110cm, exposure conditions and secure it with the tube voltage 50kVp, mAs range gives exposure to changes in 0.5,0.63,0.8,1.0,1.25,1.6,2.0,2.5,3.2mAs was within the range that can be applied to the above equipment. Depending on the conditions above, Exposure field size of 20cmx20cm, 30cmx30cm, 40x40cm, were divided into three groups. After a total of 243 times in each exposure condition, its average value was determined. Classified into three groups of the acquired image relative to the Exposure Index, and classifies the image only in the effective Exposure Index range from this calculate the DAP to use a PC-Based Monte Carlo Program 2.0 (PCXMC), derived the effective dose. Based on the effective dose obtained above test, were analyzed according to the effective dose trend Exposure field size. In addition, by measuring dose actually measured value measured by a dosimeter in each condition the actual dose value was measured by absorption at Phantom. In addition, by using the calculated PCXMC2.0 the effective dose and the probability of cancer risk from each organ. Result : Among the images included in the appropriate exposure group relative to the same mAs effective dose to increase the Field size tended to increase the effective dose is increased. On the other hand, the actually measured value of the dose measured by the dosimeter in phantom unchanged. Effective dose and cancer risk probability in each organ using PCXMC2.0 also showed a tendency to increase. Conclusion : Through the results of the classification based on the DI value of the images obtained from each condition, through the appropriate Field size adjustment of medical radiation workers, it is thought that exposure can contribute to the reduction of Radiation Protection and pediatric patients.

Perchloroethylene과 Trichloroethylene의 혐기적 탈염소화 및 미생물 군집 분석

이재원,김병혁,안치용,김희식,윤병대,오희목,Lee Jae-Won,Kim Byung-Hyuk,Ahn Chi-Yong,Kim Hee-Sik,Yoon Byung-Dae,Oh Hee-Mock 한국미생물학회 2005 미생물학회지 Vol.41 No.4

울산, 여수 등 공단지역의 토양, 하천의 저니, 해양의 준설토 둥을 이용하여 난분해성 염소화합물인 PCE (perchloroethylene)및 TCE (trichloroethylene)의 혐기성 탈염소화에 관련하는 미생물을 탐색하고 이들의 탈염소화 효율을 조사하였다. 혐기성 상호대사에 의한 탈염소화 효율을 조사하기 위해 전자공여체로 acetate를 사용하여 혐기성 회분식 실험을 실시하였으며, 이와 병행하여 분자생물학적인 기법인 16S rDNA의 PCR-Double Gradient DGGE (DG-DGGE)를 이용하여 미생물의 군집을 분석하였다. 그 결과 울산 태화강 및 여수 하남천의 저니를 접종한 경우 PCE는 $70\%,\;65\%$, TCE는 $50\%,\;45\%$의 높은 탈염소화 효율을 나타내었다. 또한 16S rDNA의 PCR을 이용한 DG-DGGE로 미생물 군집을 분석한 결과, 탈염소화 효율이 높은 지역의 저니에는 Desulfovibrio sup.의 미생물이 주로 존재함을 확인하였다. In this study, the anaerobic enrichment cultivation was performed with the sediments and the dredged soils from the cities of Ulsan, Masan, Yeosu, Gwangyang, Ansan and Seongnam. Acetate as an electron donor and PCE (perchloroethylene) or TCE (trichloroethylene) as an electron acceptor were injected into the serum bottle with an anaerobic medium. After the incubation of 12 weeks, the removal efficiency of PCE was highest at $70\%$ in the treatment with the sediment of Ulsan. Also, the bacterial community structure was analyzed by D-DGGE (double denatured gradient gel electrophoresis) through PCR-based 16S rDNA approaches. The dominant species id the anaerobic enrichment were found to belong to the genus of Desulfovibrio.

대청호에서 강우와 식물플랑크톤 군집의 관계

정승현 ( Seung Hyun Joung ),안치용 ( Chi Yong Ahn ),최애란 ( Ae Ran Choi ),장감용 ( Kam Yong Jang ),오희목 ( Hee Mock Oh ) 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.1

2001, 2003년의 7월부터 10월까지 대청호에 위치한 댐 지역에서 식물플랑크톤 군집, 환경요인 및 강우량을 조사하였다. 조사 기간동안의 월평균 강우량은 2001, 2003년 각각 91.3, 265.3 mm로 조사되었다 최대 Chl. α의 농도는 2001, 2003년 각각 45.4, 131.5 μg L^(-1)로 조사되었다. 남조류 세포수는 2003년 최대 49,000ce11s mL^(-1)로 조사되었고, 2001년은 최대 200,000cells mL^(-1) 이상으로 조사되었다. 그리고 식물플랑크톤 군집에서 남조류의 상대 풍부도는 2001년 97%, 2003년 74%였다. 2001년은 Microcystis spp.가 조사기간동안 우점종으로 조사되었고, 2003년은 Oscillatoria spp., Phormidium spp., Chroococcus spp. 그리고 Microcystis spp. 순으로 우점종의 변화를 보여줬다. 결론적으로 강우량은 식물플랑크톤의 종 다양성 및 수화현상을 일으키는 남조류 세포 밀도에 영향을 주었다. The phytoplankton community, environmental factors, and rainfall were investigated from July to October in 2001 and 2003 on Dam site in Daechung Reservoir. The monthly average rainfall in the investigated period were 91.3 and 265.3 mm in 2001 and 2003, respectively. The maximum chlorophyll a concentration was observed higher at 131.5 μg L^(-1) in 2003 than at 45.41 μg L^(-1) in 2001. The cyanobacterial number in 2001 was counted up to over 200,000 cells mL^(-1), which was much higher than the maximum number of 49,000 cells mL^(-1) in 2003. The relative abundance of cyanobacteria in the phytoplankton community was about 97% in 2001 and 74% in 2003. Microcystis spp. were absolutely dominant species in 2001, while a couple of cyanobacteria such as Oscillatoria spp., Phormidium spp. Chroococcus spp. and Microcystis spp. were dominant species in 2003. Consequently, it seemed that rainfall affected the diversity of phytoplankton species and decreased the density of bloom-forming cyanobacteria.