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안찬훈,김용욱,문흥규,이재선 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.1
We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.
In Vitro Propagation of Commonly Used Medicinal Trees in Korea
안찬훈 강원대학교 산림과학연구소 2019 Journal of Forest Science Vol.35 No.4
Forest medicinal resources, which constitute one of the non-timber forest products, have been regarded as healthy and highly valued products. To meet the increasing demand of the medicinal resources, it is necessary to improve the propagation methods of medicinal plants. In vitro propagation not only allows an opportunity for propagating plants in large numbers but also allows for enhancing the quality and quantity of the desired functional component of a plant by altering the growth factors, such as medium, carbon source, and plant growth regulators influence plant. There have been several studies of in vitro propagation methods, such as axillary bud culture, shooting, and embryogenesis, on Kalopanax septemlobus, Eleutherococcus sessiliflorus, Hovenia dulcis, and Schisandra chinensis in Korea between from 2000 through 2010. Furthermore, there have been attempts to proliferate callus and plantlets for producing useful natural compounds by using bioreactors. Here, we provide an account of the in vitro propagation methods of medicinal trees in Korea based on a review of several micropropagation studies.
In Vitro Propagation of Medicinal Herbs in Korea
안찬훈,송정호 강원대학교 산림과학연구소 2018 Journal of Forest Science Vol.34 No.1
Mass production of forest medicinal plants is related to quality control of raw medicinal materials. Plant tissue culture is an important technology to produce high-quality plant materials. Numerous factors are reported to influence the success of in vitro regeneration of medicinal plants. Embryogenesis is known to be the most effective techniques and it has developed in some medicinal plant species. Various in vitro cultural condition for direct and/or indirect somatic embryogenesis systems have developed in Epimedium koreaum, Bupleurum falcatum, Paeonia lactiflora, Chrysanthemum zawadskii, Houttuynia cordata etc. In this study, we provide the present statue and information of in vitro propagation techniques that is able to apply as an efficient system for rootstock propagation system of forest medicinal plants.
안찬훈,이현석,인지양,Jae-Seon Yi 강원대학교 산림과학연구소 2012 Journal of Forest Science Vol.28 No.4
The variation in random amplified polymorphic DNA (RAPD) markers were examined for Xanthoceras sorbifolium Bunge seeds from three plantations (Inner Mongolia [IM], Liaoning [LN], and Shandong [SD] province) in China. Mean genetic variation was measured by polymorphism percentage (42.10%) and expected heterozygosity (He=1.27). Among three populations, Shandong showed the highest values both in polymorphism percentage and heterozygosity (p=57.89; Ho=1.58; and He=1.37). Total genetic diversity value, based on the total loci, was estimated as total genetic diversity of the species (Ht)=0.27 and mean within-population genetic diversity (Hs)=0.16. UPGMA cluster analysis showed the genetic closeness between Inner Mongolia and Liaoning population, but that Shandong seems to be the separate population.
안찬훈,이현석 경상국립대학교 농업생명과학연구원 2023 농업생명과학연구 Vol.57 No.1
This study was carried out to develop an in vitro regeneration protocol for Xanthoceras sorbifolium, which has high utilization valueas a medicinal or biodiesel raw material owing to the high oil content of its seed. The induction rate of adventitious shoots from cotyledonsof immature zygotic embryos was 68.6% in MS medium with 0.5 mg l-1 benzylaminopurine (BA). In contrast, adventitious shoots wereinduced from mature seeds at a low rate (2.86%). The induction rate of adventitious shoots was more than 70% with thidiazuron (TDZ)and BA treatment. For adventitous shoots multiplication, the shoot induction rate was increased up to 94.29% on 1/2 MS medium supplementedwith combinations of BA and TDZ. The multiplicated shoots were successfully regenerated and showed normal growth and developmentthrough the in vitro root induction stage. These results will be helpful for the development of an efficient regeneration protocol for X. sorbifolium. 본 연구는 종자의 높은 유지 함량으로 인해 식・약용 및 바이오에너지 원료 소재로 활용 가치가 높은 문관과(Xanthoceras sorbifolium)의 부정아유도를 통한 기내 재분화 기술을 개발하기 위해 수행되었다. 미성숙 종자 절편을 0.5 mg l-1 6-benzylaminopurine (BA)이 포함된 MS 배지에치상하였을 때 부정아 유도율은 68.6%로 나타났으나, 성숙 종자 절편을 이용하였을 때 상대적으로 매우 낮은 비율(2.86%)로 유도되었다. 부정아유도를 위해 싸이토키닌류의 종류를 달리하여 치상하였을 때 thidazuron (TDZ)과 BA가 포함된 배지에서 70% 이상이 유도되었다. 유도된 부정아의증식을 위해서 절간을 BA와 TDZ이 혼용 처리된 배지에 치상하였을 때 최대 94.29%까지 신초 유도율이 증가되는 것을 확인하였다. 이 후 유도된신초는 정상적인 발근과정을 거쳐 식물체로 재분화되었다. 본 연구에서 제시된 문관과의 부정아 유도 방법을 이용하여 안정적인 종묘확보에 활용될수 있을 것이다.