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- 저자 : 문흥규 (검색결과 139 건)
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문흥규,노은운,하유미,심경구,Moon, Heung-Kyu,Noh, Eun-Woon,Ha, Yoo-Mi,Shim, Kyung-Ku 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.2
히어리나무 1년생 및 10년생을 재료로 액아배양을 통한 기내번식을 시험하였다. 줄기의 증식은 zeatin과 BA의 공조처리가 효과적이었으며 MS배지에 zeatin 0.5∼3.0 mg/L, 0.2 mg/L BA 처리시 주효하였다. 1년생이 10년생보다 전반적으로 증식 및 생장이 양호하였으며, 배양 6개월 후에는 10년생에서도 매월 절편 당 3개의 줄기유도가 가능하였다. 기내 줄기의 발근은 1년생은 97%, 10년생은 62%를 나타내었고, 토양이식시 1 년생 유래 배양묘는 67%, 10년생은 48%생존되어 모수령에 따른 차이를 나타냈다. 본 실험결과 히어리나무의 액아배양으로 유시, 성숙목의 대량번식이 가능함을 보여주었으나 선발개체의 효율적인 기내번식을 위해서는 재유령화의 기술개발과 토양순화율을 증진시켜야 하는 것으로 나타났다. We have developed an in vitro micropropagation system via shoot formation from axillary buds using nodal segments of Corylopsis coreana. Explants from both juvenile tree (one-year-old greenhouse stock seedlings) and mature tree (ten-years-old tree in nursery) were compared with regard to propagation efficiency. Combined treatment of both BA and zeatin were effective on shoot proliferation since the best result was obtained on MS medium supplemented with 0.5∼3.0 mg/L zeatin and 0.2 mg/L BA. Generally, juvenile explants were better in both shoot proliferation and growth than mature explants. However, as the duration of in vitro culture was proceed to 6 months, explants from mature tree also produced three shoots per explant. Distinctive differences in rooting and adaptability to soil of shoots obtained from mother trees. Whereas shoots originated from juvenile explants rooted as high as 97%, those from adult explants showed 62% rooting. Similar result was also observed in soil acclimatization. The plantlets derived from juvenile plants survived 67%, while only 48% of those from adult trees survived. The results showed a possibility of the micropropagation of Corylopsis coreana through shoot formation from axillary buds. In addition, the advance of the research still remain to enhance the frequency of acclimatization of plantlets from mature trees for practical application.
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멸종위기 식물 덩굴용담의 기내번식에 미치는 생장조절제 효과
문흥규,김선자,박소영,김용욱,이재선 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.1
Various plant growth regulators were tested for shoot proliferation of Tripterospermum japonicum, a rare and endangered species. Among the six different media tested, MS medium was the best for the shoot growth. Whereas BA, upto 3 mg/L, significantly increased shoot proliferation rate, it suppressed the rate at higher levels. Neither kinetin nor TDZ was so effective in proliferating shoots as BA. As for rooting, TDZ strongly inhibited it even at very low concentration though spontaneous rooting was frequently observed from the proliferated shoots during culture of lower concentration BA or kinetin. In contrast, shoot elongation was significantly promoted by GA3. More than 90% of the proliferated plantlets could be transplanted via cuttings into pots containing artificial soil mixture where they rooted and resumed normal growth. Most of the plants bloomed to bear fruits in the following year. 멸종위기 식물 덩굴용담의 기내증식법 개발을 위하여 기내증식 에 미치는 생장조절제의 효과를 시험하였다. 6가지의 기본배지 시 험에서 MS 배지가 줄기증식에 가장 적합한 것으로 나타났다. 싸이 토키닌 가운데 BA 처리는 3.0 mg/L 농도까지 증식에 유의적인 증 식효과를 보였고, 그 이상 농도에서는 증식이 억제되었다. Kinetin 과 TDZ는 BA 만큼 증식에 효과적이지 못했다. 발근에 있어 TDZ 는 저농도에서도 발근을 현저히 억제하였으나, BA와 kinetin은 저 농도 처리 조건에서 자발적인 발근이 이루어 졌다. 한편 지베렐릭 산 (GA3)의 처리는 줄기신장의 유의적인 효과가 있었다. 증식중인 줄기는 인공상토에 기외삽목하여 90% 이상 발근이 가능하고 정상 적인 생장이 이루어졌다. 대부분의 식물체는 토양이식 다음해에 개화되고 과실을 맺었다.
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두릅나무 15개체의 체세포배 유도 및 식물체 재분화에 미치는 유전자형의 효과
문흥규,홍용표,김용욱,이재순 한국식물생명공학회 2001 식물생명공학회지 Vol.28 No.3
Winter bud explants from 15 individual angelica tree (Aralia elata) were cultured in vitro to find out optimal conditions for somatic embryo induction as well as plant regeneration. Calli are induced and grown on MS medium supplemented with 1.0 mg/L 2,4-D for 4 weeks and subcultured on a half-strength MS medium without phytohormones to induce somatic embryos. Inter-simple sequence repeat (I-SSR) markers were analyzed with total DNAs extracted from the trees. Genotype effects on somatic embryo induction were examined by cluster analysis. Callus induction rate varied from 58.5 to 100% among the genotypes. Somatic embryo induction rate also greatly varied from 0 to 100% among the genotypes. There was a significant difference in somatic embryo induction rate even among the individual trees that showed close genetic relationships each other. This suggested that somatic embryo induction rate in Aralia elata be influenced by a few major specific genes rather than whole genomic similarity among individual trees. Four individuals of Ulneong-7, Cheju-1, Shingu and China, which are recalcitrant to somatic embryo induction, turned out to have a close genetic relationship, suggesting that both physiological and genetic factors affect somatic embryo induction. The results suggest that genotype selection be the most important factor to achieve an efficient propagation, although cultural optimization through medium and explant manipulation may also play crucial roles in somatic embryogensis as well as plant regeneration of these species.
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