저육(猪肉)과 잘 어울리는 췌장라이페이즈 억제능을 가진 한약재의 비교평가

신미래,김수현,이아름,김경조,김수지,이영철,노성수,Shin, Mi-Rae,Kim, SooHyun,Lee, AhReum,Kim, KyeongJo,Kim, SuJi,Lee, Young Cheol,Roh, Seong-Soo 대한본초학회 2017 大韓本草學會誌 Vol.32 No.5

Objectives : The aim of this study is to search Korean Medicine with the superior inhibitory effect of pancreatic lipase including a possibility as a well-matched sauce material with pork. Methods : We chose 5 samples which have the potent inhibitory effect of pancreatic lipase enzyme among 9 samples. 5 samples were Glycyrrhizae rhizoma, Cinnamomi cortex, Ganoderma lucidum, Syzygium aromaticum, and Schisandra chinensis. Animals were divided into eight groups (n=7). The experimental groups except for normal group were fed 60% high-fat diet. 5 samples were orally administered at a dose of 200 mg/kg body weight and orlistat were orally administrated at a dose of 60 mg/kg body weight for 7 days. Biochemical anaylses of 5 samples were executed based on lipid parameters such as triglyceride (TG) and total cholesterol (TC). Results : Korean Medicines with $IC_{50}$ of below $1mg/m{\ell}$ were Glycyrrhizae rhizoma, Cinnamomi cortex, Ganoderma lucidum, Syzygium aromaticum, and Schisandra chinensis. Body weight change of all drug-treated groups except Glycyrrhizae rhizoma reduced significantly during the experimental period. Orlistat treatment reduced significantly both TG and TC in serum. The significant emission of TG in fece showed in Cinnamomi cortex treatment, whereas Ganoderma lucidum and Syzygium aromaticum showed a tendency to increase without a significance. Besides, Ganoderma lucidum and Schisandra chinensis emitted significantly TC to fece. Conclusions : In conclusion, Cinnamomi cortex may exert anti-obesity effect by directly inhibiting pancreatic lipase, which would prevent the absorption of lipid from the small intestine. Accordingly, Cinnamomi cortex may suggest a high possibility as a well-matched sauce material with pork.

Veronica incana 추출물의 생물학적 활성 평가

신미래 ( Mi-rae Shin ),윤미영 ( Mi Yeong Yoon ),김민주 ( Min Ju Kim ),정일하 ( Il-ha Jeong ),안희연 ( Hui Yeon An ),정지원 ( Ji-won Jung ),노성수 ( Seong-soo Roh ) 대한본초학회 2024 大韓本草學會誌 Vol.39 No.3

Objectives : The aim of this study is to evaluate the potential biological activity of Veronica incana extracts (VIE) through in vitro, ex vivo, and in vivo experiments. Methods : In vitro, we conducted analyses on the total polyphenol (TP) and total flavonoid (TF) levels, alongside DPPH and ABTS radical scavenging activities. Ex vivo evaluations on adipose tissue measured glycerol release as a marker of lipolysis. In LPS-induced RAW 264.7 cells, we quantified nitric oxide (NO) production. Following H2O2 induction in U2OS cells, we performed mitochondrial assays such as MitoSox and MitoTracker. Moreover, Bodipy assays were conducted in 3T3-L1 cells. In vivo, we performed anti-osteoarthritis effect of VIE against monosodium iodoacetate (MIA)-induced osteoarthritis in rats. Results : The results presented encompass a myriad of models, from cell culture to animal experiments as well as ex vivo studies. VIE demonstrated high TP and TF contents, potent DPPH and ABTS scavenging activities, and regulated glycerol release. Moreover, the inhibition of NO production in LPS-induced inflammation was notably confirmed and the reduction of lipid droplets was distinctly shown. Furthermore, in H₂O₂-induced U2OS cells, MitoSox was effectively reduced while MitoTracker noticeably increased. In vivo assays confirmed a significant increase in hindpaw weight distribution (HWD) decreased by MIA after VIE treatment. Additionally, VIE inhibited serum inflammatory cytokines (TNF-α, IL-6, and IL-1β) and MDA levels in joint tissue. Conclusion : In conclusion, Veronica incana exhibited various pharmacological effects including antioxidant, anti-obesity, and anti-inflammatory properties.

H₂O₂ 유도 U2OS 세포에서 Pinus sylvestris L.의 산화적 스트레스 및 골관절염 개선 효과

신미래(Mi-Rae Shin),최정원(Jeong Won Choi),김민주(Min Ju Kim),안희연(Hui Yeon An),유지헌(Ji Heon Yu),정일하(Il Ha Jeong),유왕근(Wang Keun Yoo),Bold Sharavyn,노성수(Seong-Soo Roh) 한국식품영양과학회 2024 한국식품영양과학회지 Vol.53 No.5

This study investigated the pharmacological impact of the Pinus sylvestris L. water extract (PS) on hydrogen peroxide (H2O2)-induced oxidative stress in the human osteosarcoma cell line U2OS and a monosodium iodoacetate (MIA)-induced osteoarthritis model in rats. A MitoSoxTM indicator was used to measure mitochondrial reactive oxygen species (ROS) levels, while mitochondrial integrity was assessed using MitoTrackerTM. Moreover, we assessed oxidative stress-related markers, including sirtuin 1 (Sirt1), metal-responsive transcription factor 1 (MTF1), and 4-hydroxynonenal (4-HNE) using immunohistochemistry. We performed serum analysis and western blotting in vivo. The cell viability of U2OS cells was increased in a concentration-dependent manner, independent of H2O2 treatment. PS dramatically reduced the accumulation of mitochondrial ROS, while simultaneously reversing the decreased MitoTrackerTM levels. Additionally, both Sirt1 and MTF1 intensities were significantly elevated, whereas 4-HNE intensity noticeably decreased. In the animal experiment, the MIA treatment resulted in a reduction of hindpaw weight distribution changes. However, PS pretreatment significantly reversed this reduction. The serum levels of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-1β were significantly decreased compared to the control group. The control group, treated with only MIA, exhibited increased levels of three enzymes: Matrix metalloproteinase 13 (MMP- 13), which degrades collagen or proteoglycan, and MMP-2 and MMP-3, which degrade the non-collagen matrix components of the joints. However, the PS treatment significantly inhibited the levels of the enzymes except MMP-3. In conclusion, these results suggest that PS alleviated oxidative stress and regulated inflammatory cytokines and the expression of MMPs.

닭고기와 잘 어울리는 췌장라이페이즈 억제능을 가진 한약재의 비교평가

신미래 ( Mi-rae Shin ),안효진 ( Hyo-jin An ),이영철 ( Young Cheol Lee ),서부일 ( Bu-il Seo ),노성수 ( Seong-soo Roh ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.4

Objectives:In this study, the inhibitory activities of Korean Medicine against pancreatic lipase in vitro and biochemical analyses in vivo were measured to determine its possibility as a well-matched sauce material with chicken. Methods:The inhibitory activity on pancreatic lipase enzyme of 11 samples were evaluated in vitro and then 5 samples were selected. The activity of pancreatic lipase was investigated using orlistat as a positive control. Animals were divided into eight groups (n =7). The experimental groups except for normal group were fed 60% high-fat diet for 7 days. 5 samples were orally administered at a dose of 200 ㎎/㎏ body weight and orlistat were orally administrated at a dose of 60 ㎎/㎏ body weight for 7 days. Biochemical anaylses of 5 samples were executed based on lipid parameters analysis. Results:Korean Medicines with an IC50 of below 1 ㎎/㎏ were Scutellariae Radix, Gardeniae Fructus, Theae Folium Coptidis Radix, and Mori Cortex Radicis. Body weight change of Mori Cortex Radicis reduced significantly, however fecal triglyceride couldn`t regulate effectively. The most excellent inhibitory effect of pancreatic lipase showed in Scutellariae Radix treatment and also regulated significantly serum triglyceride and total cholesterol. Moreover, the supplementation of Coptidis Radix excreted meaningfully triglyceride to fece. Conclusions:In conclusion, Coptidis Radix may exert anti-obesity effect by directly inhibiting pancreatic lipase, which would prevent the absorption of lipid from the small intestine. Besides, Mori Cortex Radicis may led to the decrease of the body weight via the different pathway.

인삼을 함유한 약선레시피가 운동수행능력 및 항피로에 미치는 영향

김미림 ( Mi-lim Kim ),박순애 ( Soon-ae Park ),김민주 ( Min Ju Kim ),신미래 ( Mi-rae Shin ),노성수 ( Seong-soo Roh ),박해진 ( Hae-jin Park ) 대한본초학회 2024 大韓本草學會誌 Vol.39 No.3

Objective : This study examined the effects of yaksun recipe on the anti-fatigue and endurance enhancement properties in the forced swimming test (FST). Methods : The treatment groups were divided randomly into three groups: water-treated FST (control), 200 mg/kg of red ginseng-treated FST (RG200), 200 mg/kg of water extract of yaksun recipe-treated FST (YS200). After FST, an autopsy was performed, and the tissue and serum were collected. Results : The swimming exhaustion time in the RG200 and YG200 groups were significantly increased compared to the control group. The YG200 group fatigue indicators, D-Lactate, LDH(lactate dehydrogenase), creatine kinase, and ammonia content, significantly decreased compared to the control group. In addition, liver glycogen content significantly increased in the YG200 and tended to increase in RG200. Likewise, the glucose contents were significantly increased compared to the control group. The muscle damage indicators GPT (glutamic pyruvic transaminase) and BUN (blood urea nitrogen), a protein metabolite, in the YG200 group significantly decreased compared to the control group. Furthermore the concentration of liver lipid peroxidation, MDA(malondialdehyde) levels significantly decreased in the RG200 and YG200 compared to control group. Conclusions : These results suggest that YG200 can increase the endurance exercise capacity by decreasing the fatigue indicators, saving glycogen, and elevating the antioxidant defense system.

목과(木瓜)추출물이 급성 간손상 흰쥐에 미치는 효과

이진아,신미래,이지혜,노성수,Lee, Jin A,Shin, Mi-Rae,Lee, Ji Hye,Roh, Seong-Soo 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.1

Objective: This study was conducted to investigate the effect of Chaenomelis Fructus (CF) water extract on thioacetamide (TAA)-treated rats. Methods: Rats were divided into five groups: one normal group (n=8) and four with TAA-induced hepatic injury. These treatment groups were administered distilled water (n=8); silymarin 100 mg/kg (n=8); CF 100 mg/kg (n=8); and CF 200 mg/kg (n=8). In the TAA groups, the acute liver injury was induced via IP injection (200 mg/kg), and the silymarin and CF extract were then orally administered for three days. Subsequently, serum levels of GOT, GPT, and ammonia were confirmed as well as protein expressions using liver tissue. Results: In the liver injury-induced rats, CF administration reduced tissue damage and serum levels of GOT, GPT, and ammonia. In addition, CF increased the anti-oxidant proteins Nrf2, Keap1, HO-1, and catalase and significantly regulated matrix metalloproteinases (MMP-2 and MMP-9) and their tissue inhibitors (TIMP-1 and TIMP-2). Conclusions: In this animal model of liver injury induced by TAA, CF extract is determined to have a hepatoprotective effect by increasing anti-oxidant proteins that relieve damage and by regulating the expression of matrix metalloproteinases.

Cotoneaster mongolicus Pojark. 추출물의 HCl/ethanol로 유발된 위염 mice에 대한 보호효과

최정원,이진아,신미래,박해진,노성수,Choi, Jeong Won,Lee, Jin A,Shin, Mi-Rae,Park, Hae-jin,Roh, Seong-Soo 한국생약학회 2022 생약학회지 Vol.53 No.3

Cotoneaster mongolicus Pojark. (CM), in the family Rosaceae is an endemic plant to the Mongolian region (its name: Moнroл чapraй). In Mongolia, Cotoneaster species as a crude drug is mainly used for inflammatory diseases, diarrhea, and stomach indigestion. In this study, we evaluated the gastro-protective activity underlying mechanism of CM. For in vivo experiments, mice were divided into 5 groups; normal mice (Normal), gastritis mice (Control), gastritis mice treated with sucralfate 10 mg/kg (SC), gastritis mice treated with CM 100 mg/kg (CML), gastritis mice treated with CM 200 mg/kg (CMH). Gastritis was provoked by HCl/ethanol (60% ethanol in 150 mM HCl). After oral administration of each drug, HCl/ethanol was orally administered 90 mins later to induce gastritis. CM alleviated the damage to the gastric mucosa caused. As a result of confirming the expression of protein in gastric tissue through western blot, CM significantly reduced the expression of NF-κB activated due to gastritis. Also, it significantly modulated the Nrf2-Keap1 pathway. These results indicate that CM not only inhibits the nuclear metastasis of NF-𝛋B but also modulates the Nrf2-Keap1 pathway to relieve inflammation of the gastric mucosa.

대황-황금 혼합물이 만성 역류성 식도염에 미치는 효과

이진아,오민혁,신미래,노성수,박해진,Lee, Jin A,Oh, Min Hyuck,Shin, Mi-Rae,Roh, Seong-Soo,Park, Hae-Jin 한국생약학회 2022 생약학회지 Vol.53 No.1

Silymarin과 작약감초탕 병용투여의 C57BL/6 마우스 간조직 지질축적 및 염증 억제효과

최정원,조수정,신미래,박해진,Choi, Jeong Won,Cho, Su-Jung,Shin, Mi-rae,Park, Hae-Jin 대한본초학회 2022 大韓本草學會誌 Vol.37 No.5

Objective : The aim of the present study is to examine hepatic lipid-lowering and anti-inflammatory effects of silymarin combined with Jakyakgamcho-tang on non-alcoholic fatty liver disease in a high fat diet-induced obese mice model. Methods : C57BL/6 mice were divided into four dietary groups: (1) Normal, (2) Control (60% high-fat diet), (3) Control + silymarin 50 mg/kg/day (Silymarin), (4) Control + Silymarin 50 mg/kg/day + Jakyakgamcho-tang 100 mg/kg/day (SPG). After 12 weeks administration, mice were sacrificed and lipids and inflammation-related biomarkers were analyzed liver and plasma. Results : Silymarin and SPG treatments significantly lowered body and liver weights compared to the Control. Serumlipids (triglyceride (TG), total cholesterol) and pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin 1𝛽, and IL-6) concentrations were significantly lowered in the Silymarin and SPG groups than the Control group. Silymarin and SPG treatments suppressed hepatic TG level and hepatic lipid droplets compared to the Control. Theses two treatments significantly increased hepatic kinase B1 and AMP-activated protein kinase protein levels, and significantly decreased hepatic key lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase and stearyl coenzyme A desaturase 1) protein levels than the Control. SPG also significantly increased hepatic fatty acid oxidation-related protein (peroxisome proliferator-activated receptor alpha and uncoupling protein 2) levels than the Control. Conclusions: Silymarin and SPG suppressed hepatic lipid accumulation by regulating hepatic protein expression, and lowered blood pro-inflammatory cytokines concentrations though the synergic effect of silymarin and Jakyakgamchotang was not clear.

소시호탕(小柴胡湯)이 thioacetamide로 유발된 간섬유증 동물 모델에 미치는 영향

이세희,오민혁,신미래,이지혜,노성수,Lee, Se Hui,Oh, Min Hyuck,Shin, Mi-Rae,Lee, Ji Hye,Roh, Seong-Soo 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.6

Objectives: The aim of the current study was to investigate the effect of Sosiho-tang on thioacetamide (TAA)-induced liver fibrosis in mice and to elucidate its underlying mechanisms. Methods: The mice were divided into 4 groups: Normal mice (Normal), TAA-induced control mice (Control), TAA-induced and silymarin-treated (50 mg/kg) mice (Silymarin), and TAA-induced and Sosiho-tang treated (200 mg/kg) mice (SSHT). Liver fibrosis was induced via intraperitoneal injection of TAA three times a week for 8 weeks. Silymarin and Sosiho-tang were concomitantly administered for 8 weeks. Serum and liver tissues were then collected and the anti-oxidant and inflammatory protein levels in the liver tissues were evaluated using western blotting. Results: SSHT administration significantly reduced the levels of AST, ALT, ammonia, and MPO in the serum. SSHT also significantly down-regulated liver NADPH oxidase and regulated the Nrf2/Keap1 signaling pathway. SSHT treatment downregulated the liver NF-κB levels and suppressed inflammatory cytokines. SSHT treatment also decreased bile acid-related factors, such as CYP7A1 and NTCP, and fibrosis-related factors, such as α-SMA and Collagen I. Conclusions: Taken together, these data suggest that SSHT administration suppressed the progression of liver fibrosis by activating the Nrf2/Keap1 pathway and inhibiting NF-κB.