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초음파 처리 식물체의 배추좀나방 유충에 대한 섭식 저해활성 및 카틴합성 저해활성
방혜선,이완주,황석조,구태원,김선여 한국생명과학회 1999 생명과학회지 Vol.9 No.3
Effects of the ultrasonic treatment on plant represents antifeeding activity and chitin synthesis inhibition activity against diamondback moth (Plutella xylostella L.) by a leaf-dipping method at a concentration of 5,000ppm. Methanol extracts of leaves of the ultrasonic treatment on plant was sequentially partitioned with n-hexane, chloroform, ethlyacetate, butanol and water fractions, and bioassayed. Antifeeding activities were observed in n-hexane, chloroform and ethlyacetate fractions. N-hexane fraction has 94.6% of strongest antifeeding activity against P. xylostella. From the n-hexane sequentially partitioned showed H6 and H8 fractions of antifeeding activities against P. xylostella. And chitin synthesis inhibition activity against P. xylostella was obtained the methaol, n-hexane and water fractions.
상백피 품종에 따른 ${\gamma}$-Aminnobutyric acid 함량 비교
방혜선,이완주 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1
From mulberry root bark of 13 various varieties, ${\gamma}$-Aminnobutyric acid(GABA) contents were examined on HPLC analyzer. GABA contents in mulberry root bark were affected by genotypes. Average GABA content of the 13 mulberry varieties was 2.19 mg/gDW. GABA content of the varieties ranged from 1.70 to 2.62 mg/gDW. Among the three genotypes, GABA content of the Morus Lhou was higher than the other genotype. In GABA content Hongolppong was the highest with 2.62 mg/gDW on the 13 varieties of mulberry root bark.
Development of Copris tripartitus(Coleoptera:Scarabaeidae)in Two Diffenent Rearing Media
방혜선,K.G.WARDHAUGH,황석조,권오석 한국곤충학회 2003 Entomological Research Vol.33 No.3
Like many other sub-social insects, Copris tripartitus Waterhouse requires parental care during the development period in the brood balls. Mass-rearing is achieved by using indoor rearing systems and adding more dung after taking away the brood balls containing eggs. Without parental care, larvae are often affected by desiccation, as well as by fungal or sciarid fly intrusion of the brood balls. This study developed a good substitute for brood ball rearing medium. A significantly greater number of progeny survived to adulthood from broods produced by beetles reared on vermiculite medium (90.69±10.98%) than on peat moss medium (44.82±13.92%). Results of the study suggest that to protect the brood balls from desiccation and to produce healthy adults, 15-20% moisture content vermiculite must be used as brood ball rearing medium for most species reared in the laboratory.
애기뿔소똥구리(Copris tripartitus Waterhouse)의 발육에 미치는 온도의 영향
방혜선,나영은,김명현,노기안,이정택,Bang, Hea-Son,Na, Young-Eun,Kim, Myung-Hyun,Roh, Kee-An,Lee, Jung-Taek 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.3
토양의 비옥도를 높여주는 분식성 곤충으로써 우리나라에 서식하고 있는 애기뿔소똥구리(Copris tripartitus)의 발육생태를 각 온도에서 관찰하였다. 대량증식 및 환경변화 모델 적용을 위해 15, 17.5, 20, 25, 27.5 및 $30^{\circ}C$(16L:8D)에서 발육실험을 한 결과 알에서 우화까지의 발육기간은 $17.5^{\circ}C$에서 118.0일이고, $27.5^{\circ}C$에서는 49.3일이었다. 온도가 높을수록 짧은 발육기간을 보였으며, 알, 유충, 용, 알에서 우화까지의 발육영점온도와 유효적산온도는 각각 12.1, 11.2, 12.1, $9.2^{\circ}C$, 그리고 82.7, 462.0, 225.7, 947.2일도이었다. 난 및 유충의 발육단계에서 사망률은 $25^{\circ}C$에서 가장 낮게 나타나 애기뿔소똥구리의 성장에 적합한 온도는 $25^{\circ}C$이었다. Copris tripartitus Waterhouse is a coprophagus insect which enhances the soil fertility as conveying dung into the soil. We compared preimaginal development, mortality, and size of C. tripartitus at various temperature condition (15, 17.5, 20, 25, 27.5, 30). Total preimaginal periods ranged from 118.0d at $17.5^{\circ}C$ to 49.3d at $27.5^{\circ}C$. Development threshold temperature (DT) and total effective temperature for the development of egg, larva, pupa and for complete development (egg to emergence) were 12.1, 11.2, 12.1 and $9.2^{\circ}C$ and 82.7, 462.0, 225.7 and 947.2 degree days, respectively. The mortality of egg and larva was the lowest at $25^{\circ}C$. The optimum temperature for hatchability and on the development of C. tripartitus was estimated to the $25^{\circ}C$.