Malonate 에서 배양한 Acinetobacter calcoaceticus Malate Synthase 의 정제 및 그 성질

박재범,채호준,김유삼 ( Jae Bum Park,Ho Zoon Chae,Yu Sam Kim ) 생화학분자생물학회 1999 BMB Reports Vol.19 No.3

Malate synthase (EC 4.1.3.2) was induced in Acinetobacter calcoaceticus grown on malonate as a sole carbon source. The enzyme was purified by the combined methods of DEAE-Sephacel anion exchange chromatography, oxalate Sepharose 4B affinity chromatography and hydroxyapatite chromatography in an electrophoretically homogeneous form. The molecular size of the enzyme was estimated to be 75,000 dalton consisted of a single polypeptide. Optimum pH for the enzyme was about 8.0. The rate of acetyl group incorporation on variable concentraions of the substrate into malate was well fitted to a typical Michaelis-Menten kinetics. From the Lineweaver-Burk plot, K_m and V_(max) for acetyl-CoA were 1.5 × 10^(-5) M and 1 μmol/min/㎎ respectively and those for glyoxylate were 3.5 × 10^(-5) M and 0.8 μmol/min/㎎ respectively. The activity of this enzyme was inhibited by oxalate with K_i, 4 × 10^(-5) M and by glycolate with K_i, 1.4 × 10^(-4) M competitively. Pyruvate, succinate, malonate and tartrate also inhibited the enzyme activity. Antibody prepared against malate synthase from Pseudomonas fluorescens did not cross-react with the enzyme from Acinetobacter, suggesting that the enzyme isolated from two different bacteria are immunologocally different.

Pin-to-plate Type 대기압 PECVD 방법을 이용해 성장된 다중벽 탄소나노튜브의 전계방출 특성연구

박재범,경세진,염근영,Park Jae-Beom,Kyung Se-Jin,Yeom Geun-Young 한국진공학회 2006 Applied Science and Convergence Technology Vol.15 No.4

본 연구에서는 전계 방출소자로 사용하기 위한 탄소나노튜브의 합성 방법으로, pin to plate type의 대기압 플라즈마 소스를 사용한 AP-PECVD(Atmosphere pressure plasma enhanced chemical vapor deposition)를 이용하였으며, 이를 통하여 대기압에서 성장된 탄소나노튜브의 구조적 및 전기적 특성을 연구하였다. 유리 / 크롬 / 니켈을 기판으로 사용하여 $400{\sim}500^{\circ}C$ 변화 영역에서 탄소나노튜브를 성장시킨 결과 다중벽 탄소나노튜브가 얻어짐을 알 수 있었다. $500^{\circ}C$에서 성장시킨 탄소나노튜브의 경우 FT-Raman을 이용한 분석 결과 $I_D / I_G$ ratio 가 0.772 임을 관찰하였으며 TEM으로 분석결과, 내부의 그래파이트층은 15 - 20 층, 내부 직경은 10-15nm, 외부 직경은 30 - 40nm 이고, 각 층간의 간격은 0.3nm 임을 알 수 있었다. 또한 전계 방출 문턱전압은 $2.92V/{\mu}m$ 이고, FED 에서 요구되는 $1mA/cm^2$의 방출전류밀도는 $5.325V /{\mu}m$의 문턱전압 값을 가지는 것을 관찰하였다. In this study, carbon nanotubes (CNTs) were grown on glass substrates coated with Ni/Cr by an atmospheric pressure plasma enhanced chemical vapor deposition(AP-PECVD) and their structural and electrical characteristics were investigated as a possible application to the field emitter of field emission display (FED) devices. The substrate temperature ($400{\sim}500^{\circ}C$) were varied and the grown CNTs were multi wall CNTs (at $500^{\circ}C$, 15 - 20 layers of graphene sheets, distance of each layer : 0.3nm, inner diameter: 10 - 15nm, outer diameter: 30 - 40nm). The ratio of defective carbon peak to graphite carbon peak of the CNTs grown at $500^{\circ}C$ (C measured by fourier transform(FT)-Raman was 0.772 $I_D / I_G$ ratio. When field emission properties were measured, the turn-on field was $2.92V/{\mu}m$ and the emission field at $1mA/cm^2$ was $5.325V /{\mu}m$.

Purification and Characterization of Malate Synthase from Acinetobacter calcoaceticus Grown on Malonate

박재범,채호준,김유삼,Park, Jae-Bum,Chae, Ho-Zoon,Kim, Yu-Sam 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3

Malonate를 유일한 탄소 및 에너지원으로 이용하는 배지에서 Acinetobacter calcoaceticus를 배양했을 때 malate synthase가 유도된다는 사실을 확인하였다. 이 미생물로부터 DEAE-Sephacel ion exchange chromatography, oxalate-Sepharose 4B affinity chromatography, hydroxyapatite chromatography를 이용하여 malate synthase를 분리 정제하였고, disc polyacrylamide gel electrophoresis를 수행한 결과 하나의 단백질 띠로 나타났다. 그 최적 pH는 8.0이였으며, 정제한 malate synthase가 관여하는 반응은 기질인 glyoxylate나 acetyl CoA에 대해 전형적인 Michaelis-Menten kinetics에 부합했다 Lineweaver-Burk plot을 이용하여 $K_m$과 $V_{max}$를 정한 결과 acetyl CoA에 대해서는 $1.5{\times}10^{-5}\;M$과 $1\;{\mu}mol/min/mg$ protein이였고 glyoxylate에 대해서는 $3.5{\times}10^{-5}\;M$과 $0.8\;{\mu}mol/min/mg$ protein 이었다. Oxalate와 glyoxylate는 competitive inhibitor였으며 $K_i$ 값은 각각 $4{\times}10^{-5}\;M$, $1.4{\times}10^{-4}\;M$이었다. Pyruvate, succinate, malonate 그리고 tartrate도 효소활성을 억제하였다. Pseudomonas fluorescens의 malate synthase를 항원으로 하여 만들어진 항체는 Acinetobacter calcoaceticus 의 malate synthase활성을 저해 하지 않았다. 이러한 사실로 미루어 분류상 유사한 두 미생물의 malate synthase는 면역학적으로 많은 차이가 있음을 알았다. Malate synthase (EC 4.1.3.2) was induced in Acinetobacter calcoaceticus grown on malonate as a sole carbon source. The enzyme was purified by the combined methods of DEAE-Sephacel anion exchange chromatography, oxalate Sepharose 4B affinity chromatography and hydroxyapatite chromatography in an electrophoretically homogeneous form. The molecular size of the enzyme was estimated to be 75,000 dalton consisted of a single polypeptide. Optimum pH for the enzyme was about 8.0. The rate of acetyl group incorporation on variable concentraions of the substrate into malate was well fitted to a typical Michaelis-Menten kinetics. From the Lineweaver-Burk plot, $K_m$ and $V_{max}$ for acetyl-CoA were $1.5{\times}10^{-5}\;M$ and $1\;{\mu}mol/min/mg$ respectively and those for glyoxylate were $3.5{\times}10^{-5}\;M$ and $0.8\;{\mu}mol/min/mg$ respectively. The activity of this enzyme was inhibited by oxalate with $K_i$, $4{\times}10^{-5}\;M$ and by glycolate with $K_i$, $1.4{\times}10^{-4}\;M$ competitively. Pyruvate, succinate, malonate and tartrate also inhibited the enzyme activity. Antibody prepared against malate synthase from Pseudomonas fluorescens did not cross-react with the enzyme from Acinetobacter, suggesting that the enzyme isolated from two different bacteria are immunologocally different.

소유역별 첨두유량 기여율을 고려한 홍수지수 산정 및 홍수위험 분석

박재범,강두기,신현석,Park,Jaebeom,Kang,Dugi,Shin,Hyeonseok 한국방재학회 2014 한국방재학회 학술발표대회논문집 Vol.2014 No.1

홍수피해는 일반적으로 주수로 상의 하도를 따라 집중하여 발생한다. 홍수관리 최적 대안을 선정하기 위해서는 피해가 발생하는 하도뿐만이 아니라 하류 말단지점의 첨두유량에 영향을 미치는 개별 소유역의 기여율을 고려해야 한다. 본 연구에서는 하류 말단지점의 첨두유량에 대한 개별 소유역의 기여율 산정을 위한 반복적인 모의 기법을 개발하고 이를 기반으로 하는 홍수지수를 제안하였다. 홍수 지수는 유출해석 모형에서 임의 소유역을 제외하였을 경우 첨두유량에 대한 해당 소유역의 기여율과 단위면적당 기여율을 의미한다. 수영강을 20개 소유역으로 구분하고 Hec-HMS 모형을 구축하였으며 설계빈도에 해당하는 임계지속시간 24시간 100년 빈도 강우를 적용하여 기여율을 기반으로 하는 홍수지수를 산정하였다. 기여율을 기반으로 하는 홍수지수는 유역의 유출특성을 지배하는 유역면적, 유역경사, 하천경사, 불투수율 등에 대한 종합적인 영향을 포함하고 있는 것으로 검토되었다.

50KW 태양광발전의 원격 감시제어시스템에 관한 연구

박재범,김병목,신건,노대석,Park, Jeabum,Kim, Byungmok,Shen, Jian,Rho, Daeseok 한국융합학회 2011 한국융합학회논문지 Vol.2 No.3

본 논문에서는 배전지능화시스템과 정보 연계, 미래 지능형전력망 운용 및 태양광, 풍력, 연료전지등과 같은 신에너지전원의 단지를 효율적으로 유지관리하기 위하여, 유 무선 네트워크 구성요소의 특성을 해석하고, 이를 바탕으로 원거리감시제어 장치를 제작하였다. 이 장치는 50KW급 태양광전원과 유 무선 네트워크의 알고리즘을 기반으로 실시간으로 출력되는 전압, 전류데이터를 무선네트워크로 장거리 전송하여, HMI 프로그램인 Autobase로 실시간 모니터링 하도록 하였다. 따라서 본 논문에서는 제안한 원거리 감시제어 장치를 이용하여 50KW급 태양광 발전 데이터를 무선네트워크 기술로 전송받아 데이터를 측정하고, 이 결과를 분석하여 태양광발전량의 운용특성을 분석하였다. This paper deals with the efficient management for the intelligent distribution system related with the renewable energy sources, using the wire-wireless monitoring and control device. This device is mainly composed of 2 sections. One is monitoring device with the Autobase S/W and the other is control device using PLC. This paper proposes a wire and wireless monitoring and control device which can monitor and control the 50Kw PV system installed remotely (about 1Km) in the campus of the Korea University of Technology and Education. By the analysis of PV output characteristic using the device proposed in this paper, it is confirmed that the device can contribute the maintenance of PV system and also the establishment of Smart Grid.

낙동강 중소하천 홍수예경보시스템 구축을 위한 한계강우량 산정과 적용

박재범,신동수,박무종,강봉권,신현석,Park,Jae Beom,Shin,Dong Soo,Park,Moo Jong,Kang,Bong Gwon,Shin,Hyun Suk 한국방재학회 2013 한국방재학회논문집 Vol.13 No.2

본 연구에서는 중소하천 홍수경보시스템 구축을 위한 소유역기반 한계강우량 산정 방법론을 제안하였다. 소유역 말단 하천단면에서 위험유량에 해당하는 한계강우량을 초과하는 강우가 발생하면 위험상황이 발생하고 하천재해에 대응하고 대처하기 위한 경보를 발령한다. 관측강우량과 유역의 수문학적반응특성을 기반으로 하는 한계강우량은 소유역 말단에서 위험상황을 발생하는 유량에 해당하는 특정 지속시간 동안의 총강우량을 의미한다. 지형분석도구인 ArcGIS 기반 Hec-GeoHMS를 이용하여 낙동강유역을 2,268개 소유역으로 분할 후 Hec-HMS모형을 구축하였으며 이를 이용하여 선행토양함수조건과 초기손실 시나리오에 따른 한계강우량을 산정하였다. An procedure for the definition of rainfall threshold value is illustrated in order to develop optimal flood forecasting and warnings systems for small river basins. Threshold overcoming could produce a danger situation in river sites and trigger the prevention and emergency system alert. Rainfall threshold values is based both on the quantitative rainfall observed and the hydrological response of the small river basin. Threshold values specify the rainfall amount for a given duration that generates a danger discharge in a outlet`s cross section and are estimated by hydrological modeling for several scenarios(e.g, Soil Moisture conditions, Initial Abstraction). Nakdong River basin is separated to 2,268 small river basins by using Hec-GeoHMS, rainfall threshold for small river basins are presented for several scenarios.

생지황(生地黃)이 강제수영부하시험에서 HPA Axis System과 Catecholaminergic System에 미치는 영향

박재범,이태희,Park, Jae-Beom,Lee, Tae-Hee 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Object : The effects of Rehmanniae Radix(RR) on HPA Axis system and Catecholaminergic system was investigated. Methods : we performed the Forced Swimming Test(FST). Also the expression of Corticotropin -Releasing Factor(CRF), c-Fos and Tyrosine Hydorxylase(TH) was measured by immunohistochemical method at Paraventricular Nucleus(PVN), Locus Coeruleus(LC) and Ventral Tegmental Area(VTA). Results : 1. The duration of immobility in the Forced Swimming Test was significantly decreased in the Rehmanniae Radix 400 mg/kg groups in comparison with the control group(p<0.05). 2. Comparing to the control group, CRF expression was significantly decreased in the Rehmanniae Radix 400 mg/kg treated group in comparison with the control group(p<0.01). 3. c-Fos expression was significantly decreased at PVN in the Rehmanniae Radix 100 mg/kg treated group in comparison with the control group(p<0.01). 4. TH expression was significantly increased at LC in the Rehmanniae Radix 100 mg/kg and 400 mg/kg treated group in comparison with the control group(p<0.001). 5. TH expression was significantly decreased at VTA in the Rehmanniae Radix 100 mg/kg and 400 mg/kg treated group in comparison with the control group(p<0.001). Conclusion : According to the results, it can be considered that Rehmanniae Radix has antidepressant effect by showing the reduction of immobility in FST through the decreased expression of CRF, c-Fos in PVN and TH in VTA.

다중이용시설 내부에 분포하는 부유 진균의 입경별 농도 특성

박재범,김기연,장규엽,김치년,이경종,Park, Jae-Beom,Kim, Ki-Youn,Jang, Gyu-Yeob,Kim, Chin-Yon,Lee, Kyung-Jong 한국환경보건학회 2006 한국환경보건학회지 Vol.32 No.1

The aim of this study is to examine size-based concentration and genera of airborne fungi distributed in public facilities such as hospital, kindergarten, day-care center and postpartum nurse center and to provide fundamental data in order to prevent respiratory diseases caused by exposure to airborne fungi. Culturable total and respirable concentrations of airborne fungi averaged to $382\;cfu/m^3\;and\;292\;cfu/m^3$ in hospital, $536\;cfu/m^3\;and\;347\;cfu/m^3$ in kindergarten, $334\;cfu/m^3\;and\;266\;cfu/m^3$ in day-care center, and $371\;cfu/m^3\;and\;289\;cfu/m^3$ in postpartum nurse center, respectively. The ratio of respirable to total concentration of airborne fungi in the investigated public facilities was ranged from $55\%\;to\;70\%$ but there was no significant difference among them (p>0.05). The mean I/O ratio of culturable total and respirable concentrations were 0.56 and 0.64 in hospital, 0.72 and 0.91 in kindergarten, 0.33 and 0.45 in day-care center, and 0.63 and 0.73 in postpartum nurse center, respectively. Indoor concentration of airborne fungi did not correlated significantly with indoor temperature and relative humidity (p>0.05) but had a significant positive correlation with $CO_2$ concentration (p<0.01) and surrounding condition (p<0.05). Penicillium spp., Cladosporium spp., and Aspergillus spp. were estimated to over $95\%$ of total airborne fungi identified in the investigated public facilities.

기업체 화학물질관리 우수사례

박재범,Park, Jae-Beom 환경보전협회 2007 환경정보 Vol.29 No.367

불량한 치조제에서의 임플랜트 시술증례

박재범,안상헌,정수일,조병완,안재진,Park, Jae-Bum,Ahn, Sang-Hun,Cheung, Soo-Il,Jo, Byung-Woan,Ahn, Jae-Jin 대한악안면성형재건외과학회 1997 Maxillofacial Plastic Reconstructive Surgery Vol.19 No.1

The most critical factor in determining which type of implant to be used would be the available bone of the patient. Usually a minimum of 5mm in the bone width and 8mm in the bone height is necessary to ensure primary implant stability and maintain the integrity of bone contact surface. Placement of implant is limited by the several anatomic strutures such as maxillary sinus, floor of the nose, inferior alveolar neurovascular bundle and nasopalatine foramen, etc. When severe resorption of alveolar ridge is encountered, implant placement would be a problematic procedure. A number of techniques to improve the poor anatomic situations have been proposed. This article reports 4 cases of patients using surgical procedures such as blade implant technique, cortical split technique in the anterior maxillary area, sinus lifting and lateral repositioning of inferior alveolar nerve, We treated dental implant candidates with unfavorable alveolar ridge utilizing various surgical techniques, resulted in successful rehabilitation of edentulous ridge.