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RISS 인기검색어
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- 저자 : 김희선(Kim, Hee Sun) (검색결과 785 건)
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김희선,설혜원,안희진,오선경,구승엽,김석현,최영민,김정구,문신용,Kim, Hee-Sun,Seol, Hye-Won,Ahn, Hee-Jin,Oh, Sun-Kyung,Ku, Seung-Yup,Kim, Seok-Hyun,Choi, Young-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.4
Objective: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after karyotyping. Method: The hAFC was prepared for feeder layer in the presence of Chang's medium and STO medium (90% DMEM, 10% FBS) at $37^{circ}C$ in a 5% $CO_2$ in air atmosphere. Prior to use as a feeder layer, hAFC was mitotically inactivated by mitomycin C. The hESCs on hAFC were passaged mechanically every seven days with ES culture medium (80% DMEM/F12, 20% SR, bFGF). Results: The hAFC feeder layer support the growth of undifferentiated state of SNUhES2 for at least 59 passages thus far. SNUhES2 colonies on hAFC feeder appeared slightly angular and flatter shape as compared with circular and thicker colonies observed with STO feeder layer and showed higher level with complete undifferentiation in seven days. Like hESC cultured on STO feeders, SNUhES2 grown on hAFC expressed normal karyotype, positive for alkaline phosphatase activity, high telomerase activity, Oct-4, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 and formed embryoid bodies (EBs). Conclusion: The hAFC supports undifferentiated growth of hESC. Therefore, these results may help to provide a clinically practicable method for expansion of hESC for cell therapies.
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동결보존시 생쥐 전핵배아의 시기에 따른 생존율과 발생율의 비교
김희선,류범용,오선경,서창석,김석현,최영민,김정구,문신용,이진용,Kim, H.S.,Ryu, B.Y.,Oh, S.K.,Suh, C.S.,Kim, S.H.,Choi, Y.M.,Kim, J.G.,Moon, S.Y.,Lee, J.Y. 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.1
This study was designed to evaluate the influence of pronuclear age on the survival and post-thawing development after cryopreservation of mouse embryos. Freezing and thawing were performed in the different pronuclear stages of mouse embryos after IVF. Embryos were obtained from $F_1$ hybrid mice and classified into 4 groups according to the pronuclear stage (6hr, 9hr, 12hr and 15hr after insemination). Pronuclear ova were slowly cooled in a biological freezer using 1.5M 1,2-propanediol and 0.1M sucrose as cryoprotectant. Thawing was done at room temperature and 1,2-propanediol was removed by multi-step dilutions. Both frozen-thawed embryos and control fresh embryos were cultured in vitro in Ham's F-10 medium supplemented with 4mg/ml BSA. In control group, the development rate after 48hr was 99.3%, and the complete hatching rate after 144hr was 61.3%. In experimental groups, the survival rate after thawing was 95.4% in 6hr, 88.7% in 9hr, 75.2% in 12hr and 62.4% in 15hr after insemination, the development rate after 48hr was 61.1, 77.0, 67.0 and 79.6%, respectively, and the complete hatching rate after 144hr was 25.7, 43.7, 42.2 and 60.0%, respectively. The survival rate in 15hr was significantly lower (p<0.05) compared with other groups. In vitro development rates after 48hr were similar in all groups, but complement hatching rate was significantly lower (p<0.05) in 6hr group. In conclusion, cryopreservation of mouse pronuclear ova with 2 distinct pronuclei (9hr and 12hr groups) showed better results after thawing compared with early (6hr group) or late pronuclear ova just prior to cleavage (15hr group).
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하리자돈(下痢子豚) 분변(糞便)에서 Rotavirus 분리(分離) 동정(同定)
김희선,최정옥,김두희,유영수,안수환,Kim, Hee-sun,Choi, Chung-ok,Kim, Doo-hee,Lyoo, Young-soo,An, Soo-hwan 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1
Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in chonnam province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranged from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera.
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생쥐 8세포배의 Compaction에 미치는 칼슘 Inhihitor의 영향
김희선,배인하,Kim, Hee-Sun,Bae, In-Ha 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1
In mammalian development, the embryo which is in the process of compaction, involves a progressive flattening of blastomeres against each other with the consequence that the embryo assumes a spherical shape. This stage happens in the first differentiation. The present study was aimed to examine the involvement of other metal ions in compaction by treating with various divalent cations in the absence of $Ca^{++}$. When 8-cell embryos were cultured in $Ca^{++}$-free medium for 24hrs, they developed to 16-cell stage but did not compaction, and degenerated after 48hrs of culture. Embryos were cultured in $Ca^{++}$-free medium for 24hrs and then transferred to the control medium showed the normal compaction afterwards. When 8-cell embryos were cultured in the presence of $Ni^{++}$, known as a $Ca^{++}$ inhibitor, they cleaved to 16-cell stage but did not compact in the absence of $Ca^{++}$. On the other hand, embryos cultured in the media containing both $Ca^{++}$ and $Ni^{++}$ developed normally so that they underwent compaction during culture for 48hrs. However, they failed to hatch during further 24hrs in the same medium, indicationg that $Ni^{++}$ may exert some harmful effects. Embryos grow in the control medium that contained $Ca^{++}$ but not $Ni^{++}$, developed to the hatched blastocysts. The treatment with $Cd^{++}$ $10^{-1}$,$10^{-2}{\mu}M$, $Mn^{++}$ or $Ba^{++}$ 10,100, $1000{\mu}M$ in $Ca^{++}$-free medium, respectively, inhibited compaction and embryonic degeneration began as in $Ca^{++}$-free medium. When 3, 5, 10mM of $Sr^{++}$, known as a substitute for $Ca^{++}$ in cell, was added to $Ca^{++}$-free medium, respectively, compaction was induced unlike the above metal ions. Embryos were cultured in $Sr^{++}$ developed to blastocysts, but failed to hatch after 72hrs and degenrated. On the other hand, when embryos were cultured in 3, 5, 10mM of $Sr^{++}$ but in $Ca^{++}$-free medium for 24hrs respectively and then transferred to the control, they showed the similiar development as that in the control.
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김희선,여인권,Kim, Hee-Sun,Yeo, In-Kwon 한국통계학회 2011 응용통계연구 Vol.24 No.6
주가연계증권(ELS)은 주가와 연동되어 수익률이 결정되는 금융상품으로 현재 증권회사뿐만 아니라 은행에서도 많이 판매되고 있는 간접투자상품이다. 이 논문에서는 수익다이어그램을 통해 대표적인 주가연계증권의 수익구조를 알아보며 특히 현재 가장 많이 판매되고 있는 원금비보장 상품인 스텝다운형에 관심을 가진다. KOSPI 200과 홍콩 HSI를 기초자산으로 설정한 스텝다운형 상품에서 투자시점의 시계열 특성에 따른 조기상환여부와 경험적 분포를 이용한 VaR를 통한 위험률에 대해 고찰해 본다. Equity linked securities are indirect investments where the return of investment depends on the performance of the underlying equities. In this paper, we review the profit structure of typical equity linked securities through a profit diagram and investigate which characteristics of time series at the investment affect the early repayment of the stepdown ELS based on KOSPI 200 and HSI. We also compare VaRs using the empirical distribution function for risk management.
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김희선,강문일,이채용,김성호,Kim, Hee-sun,Kang, Mun-il,Lee, Chai-yong,Kim, Sung-ho 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4
One hundred twelve domestic dog sera were collected from Kyonggi, Kanngwon, Cholla, and Kyongsang province. All the sera were examined by immunofluorescence antibody test(IFAT) against scrub typhus, murine typhus and spotted fever group rickettsia infection. The antibody prevalence was 5.40%(6/112) against scrub typhus. The sero-prevalence rates of scrub typhus were 16.30%(5/31) in Cholla and 5.90%(1/17)in Kyonggi areas. The prevalence of scrub typhus was higher in older dogs that were 6 years or more. The antibody prevalence was 8.04%(9/112) against murine typhus. The sero-positive rates to murine typhus were 5.88%(1/17) in Kyonggi, 8.33%(2/24) in Kangwon, 9.68%(3/31) in Cholla, and 7.50%(3/40) in Kyongsang areas, respectively. The sero-positive dogs to murine typhus were widely distributed throughout all age groups from 2 months to above 9 years old. The antibody prevalence was 16.96%(19/112) for spotted fever group rickettsial infection. The sero-positive rates of spotted fever group rickettsia were 11.76%(2/17) in Kyonggi, 12.90%(4/31) in Cholla, and 32.50%(13/40) in Kyongsang areas. The sero-positive dogs to spotted fever group rickettsia were observed at all age groups of dog. The sero-positive prevalence between sex showed higher in female than in male except for spotted fever group rickettsia infection.
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