만성 알코올 유발 마우스 간손상 및 지방 축적에 대한 제주조릿대잎 에틸 아세테이트 분획물의 간 보호 효과

김아름,이영주,김효진,양지원,김주성,지영흔,Kim, Areum,Lee, Youngju,Herath, Kalahe Hewage Iresha Nadeeka Madushani,Kim, Hyo Jin,Yang, Jiwon,Kim, Ju-Sung,Jee, Youngheun 대한수의학회 2020 大韓獸醫學會誌 Vol.60 No.4

Sasa (S.) quelpaertensis Nakai (Korean name, Jeju-Joritdae), which has anti-oxidative and anti-inflammatory activities, is a type of bamboo grass distributed widely in Jeju Island, Korea. S. quelpaertensis leaves are used for therapeutic purposes in traditional Korean medicine. This study examined the hepatoprotective effects of the S. quelpaertensis ethyl acetate fraction (SQEA) in a mouse model to mimic alcoholic liver damage. The mice were administered orally with 30% alcohol (5 g/kg) once per day with or without SQEA treatments (100 and 200 mg/kg) for 14 days consecutively. Alcohol consumption increased the serum alcohol content and histopathological changes but reduced the liver weight. Moreover, the livers of the alcohol group exhibited the accumulation of malondialdehyde and cytochrome P450 2E1 (CYP2E1), and lipid droplet coating protein perilipin-2. On the other hand, SQEA dose-dependently attenuated the alcohol-induced serum ethanol content and liver histopathological changes but increased the liver weight. Moreover, SQEA attenuated the level of CYP2E1 and inhibited alcohol-induced lipogenesis in the liver via decreased perilipin-2 expression. These results suggest that SQEA can provide a potent way to reduce the liver damage caused by alcohol consumption.

방사선을 조사한 마우스에서 비장세포에 대한 톳의 보호 작용

김아름,빙소진,조진희,안긴내,이지혁,전유진,이병걸,지영흔,Kim, Areum,Bing, So Jin,Cho, Jinhee,Ahn, Ginnae,Lee, Ji-Hyeok,Jeon, You-Jin,Lee, Byung-Gul,Jee, Youngheun 대한수의학회 2015 大韓獸醫學會誌 Vol.55 No.1

The immune system is specifically sensitive to oxidative stress induced by ionizing radiation because of its rapid proliferative activity. For this reason, an instructive immune system is one of the best ways to minimize side effects, such immunodeficiency, of gamma radiation. Over the past few decades, several natural plants with antioxidant and immunomodulatory properties have been identified as adjuncts for nontoxic and successful radiotherapy. Hizikia fusiforme extract (HFE) containing plentiful dietary fiber and fucoidan is known for its instructive antioxidant capacity, immunomodulation abilities, and immune activation. In this study, we determined whether HFE protects radiosensitive immune cells from gamma radiation-induced damage. C57BL/6 mice were irradiated with gamma-ray. The effect of HFE on the ionizing radiation damage of immune cells was then evaluated with an MTT assay, 3H-thymidine incorporation assay, and PI staining. We found that HFE stimulated the proliferation of gamma-ray irradiated immune cells without cytotoxic effects. We also observed that HFE not only decreased DNA damage but also reduced gamma radiation-induced apoptosis of the immune cells. Our results suggest that HFE can protect immune cells from gamma-ray damage and may serve as an effective, non-toxic radioprotective agent.

PVA 중합도가 고상-액상 혼합 방식에 의한 YAG:Ce³⁺ 형광체 분말 합성에 미치는 영향

김아름,이상진,Kim, A-Reum,Lee, Sang-Jin 한국세라믹학회 2014 한국세라믹학회지 Vol.51 No.5

YAG:$Ce^{3+}$ phosphor powders were synthesized using $Al(OH)_3$ seeds by means of a PVA-polymer-solution route. Various types of PVA with different molecular weights (different polymerization) were used. All dried precursor gels were calcined at $500^{\circ}C$ and then heated at $1500^{\circ}C$ in a mix of nitrogen and hydrogen gases. The final powders were characterized via XRD, SEM, PSA, PL, and PKG analyses. The phosphor properties and morphologies of the synthesized powders were dependent on the PVA type. As the molecular weight of the PVA was increased, the particle size gradually decreased with agglomeration, and the luminous intensity of the phosphor increased. However, the phosphor powder prepared from the PVA exhibiting very high molecular weight, showed a 531 nm (blue) shift from the 541 nm (yellow) wavelength of the YAG:$Ce^{3+}$ phosphor. Finally, the synthesized YAG:$Ce^{3+}$ phosphor powder prepared from the PVA with 89,000 - 98,000 molecular weight showed phosphor properties similar to those of a commercial phosphor powder, but without a post-treatment process.

방사선으로 인한 산화적 손상에서 phloroglucinol의 모낭 보호 효과

김아름,빙소진,조진희,전유진,이병걸,박재우,지영흔,Kim, Areum,Bing, So Jin,Cho, Jinhee,Herath, KHINM,Jeon, You-Jin,Lee, Byung-Gul,Park, Jae-Woo,Jee, Youngheun 대한수의학회 2016 大韓獸醫學會誌 Vol.56 No.1

When exposed to gamma-rays, hair follicular cells immediately go through apoptosis, which hampers their rapid differentiation essential for the regeneration of hair. Phloroglucinol (PG) is a phenolic compound of Ecklonia cava, brown algae abundant in Jeju island, Korea. Containing plentiful polyphenols, PG is known for its instructive effects by inhibiting apoptosis, scavenging oxygen radicals, and protecting cells against oxidative stress. In this study, we demonstrate that PG rescues radiosensitive hair follicular cells from gamma radiation-induced apoptosis and DNA damage. To identify protective capacity of PG on hair follicles, we irradiated with 8.5 Gy (1.5 Gy/min) of gamma-rays to the whole body of C57BL/6 mice at day 6 after depilation with or without PG. In mice exposed to radiation, the expression of proapoptotic molecule p53 was downregulated in the skin of PG treated group. On immunohistochemical observation of the skin, PG inhibited the immunoreactivity of p53 and cleaved caspase-3. PG treatment protected hair follicular cells from cell death due to gamma-radiation. Our results suggest that PG presents radioprotective effects by inhibiting apoptosis of radiosensitive hair follicular cells and can protect hair follicular cells from gamma-ray induced damage.

교통소외지역 자율주행 모빌리티 서비스를 위한 도로 환경 분석 : 대구광역시와 세종특별자치시를 중심으로

김아름,우보람,탁세현,임서현,Kim, Ahreum,Woo, Boram,Tak, Sehyun,Lim, Seohyun 한국ITS학회 2022 한국ITS학회논문지 Vol.21 No.5

The introduction of Connected and Automated(CAD) technology has a growing interests especially in areas with low demand for transport, where often the local transport services do not connect to peripheral areas for more efficient and convenient mobility services. However, there are few researches on CAD-based mobility services in areas with low demand for transport. Because current researches are mainly focuses on introduction of CAD-based mobility services in area with high demand for transport such as urban and highway. These two areas have different road environments and these differences require different driving technology especially related to Operational Design Domain(ODD). ODD is important factors for introduction of CAD-based mobility services. Therefore, we compare the road environments of areas with low demand for transport and areas with high demand for transport in terms of ODD. In addition, this paper proposes suggest guidance for the introduction of CAD-based mobility services in areas with low demand for transport such as rural and suburban areas.

뉴타운사업 내 재개발구역의 주체별 개발이익 비교 분석 - 길음 뉴타운 2, 4구역을 중심으로 -

김아름,구자훈,김현진,Kim, Ah-Leum,Koo, Ja-Hoon,Kim, Hyun-Jin 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.5

This New-Town Project has been proposed to improve the environments of deprived residential areas. This paper investigates the distribution levels of the participating parties' monetary surpluses from New-Town Projects through a case study and tentatively suggests political issues and measures. The methodology followed includes a thorough survey of the precedent theories, including a literature review concerning issues and tasks that have been raised since the development was initiated. A general assessment of the complete project is also provided with a broad perspective of the background settings. Secondly, the case study of Gil-Eum will be the focus for identifying the mechanism for the project's profit distribution levels, then for constructing a set of measuring procedures according to the stages of the project and the participating parties. This set of procedures that has been drawn out from recognizing the system, is applied to the target project and measures the amount of profit gained from each stage of the development. This also aids in collating information about the procedural arguments concerning the financial gains of the operations that have been provided. This project falls under the 'Urban and Residential Environment Improvement Act', and based on the results of each stage, the measurement of the profits of the participating parties is analyzed.

흰쥐의 뇨 중 3,5,6-Trichloro-2-Pyridinol 분석을 통한 Chloropyrifos 노출량 예측

김아름누리(Areumnuri Kim),백민경(Min-Kyoung Paik),이선영(Sun-Young Lee),홍수명(Su-Myeong Hong),정미혜(Mihye Jeong),오진아(Jin-A Oh) 한국농약과학회 2019 농약과학회지 Vol.23 No.4

Chlorpyrifos (CPF) is one of the organophosphates (OPs) pesticides, and has been widely studied for pesticide exposure by agricultural operator due to its high chemical penetration rate. However, studies to predict the exposure of acute CPF through the analysis of its metabolites using the biomonitoring for agricultural operators are insufficient. In this study, we examined the urinary excretion of 3,5,6-Trichloro2-Pyridinol (TCP) following the repeated exposure of CPF through the skin of rats and confirmed the correlation between CPF exposure and urinary TCP excretion, and finally predicted the CPF exposure by urinary TCP excretion in rats. TCP excretion was analyzed in urine samples of rats exposed through the skin for three times at low (1.01 ㎎/㎏), medium (10.05 ㎎/㎏) and high concentrations (100.50 ㎎/㎏) every 7 days. As the number of exposures increased, urinary TCP excretion decreased significantly, and the decrease in TCP excretion in urine increased with increasing exposure concentration. The correlation between dermal exposure of CPF and their excretion of TCP in urine samples were high, ranging from 0.67 to 0.86. In addition, the absorbed CPF ratio was 4.20~18.81% in rats of low-CPF exposed levels, which was selected as the realistic pesticide exposure level for the agricultural field in Korea. It would be considered to be a basic data for predicting acute pesticide exposure of agricultural operators through TCP analysis using biological samples for farmers in agricultural field.

Deltamethrin의 경피반복노출에 따른 흰쥐의 3-PBA 배설과의 상관관계

김아름누리(Areumnuri Kim),전경미(Kyongmi Chon),박병준(Byung-Jun Park),문병철(Byeong-Chul Moon),김병석(Byung-Seok Kim),백민경(Min-Kyoung Paik) 한국농약과학회 2018 농약과학회지 Vol.22 No.4

Deltamethrin, one of the Pyrethroids (PYRs) pesticides, has been widely used in agriculture and industry. The study on human metabolism and excretion of deltamethrin is needed since low level of pesticide is exposed repeatedly in agricultural fields. This study is aimed to analyse urinary 3-Phenoxybenzoic acid (3-PBA) in male rats following three times of dermal exposure to deltamethrin with interval of 7 days; low (0.0705 mg/kg bw), medium (0.7050 mg/kg bw) and high (7.0500 mg/kg) level of exposure, and then investigate the time courses of urinary 3-PBA and dermal deltamethrin exposure. As a result, the urinary 3-PBA excretion in both concentration and amount were eliminated with increasing dermal exposures in all levels of deltamethrin exposure groups. The low level of deltamethrin groups showed especially significant decrease in urinary 3-PBA. Likely, the more exposure level of deltamethrin, the more urinary 3-PBA excretion showed high correlation between the repeated dermal exposure to deltamethrin and urinary 3-PBA excretion. This result is similar with the results when the number of repetition increases. Base on the results, accumulation of deltamethrin in agricultural workers who generally exposure to low levels of chronic pesticide may be negligible in body. We suggested that urinary 3-PBA excretion may be acute biomarker available to evaluate dermal exposure to deltamethrin.

The Correlation Between Deltamethrin Exposure and Urinary 3-PBA Concentrations in Rats

김아름누리 ( Areumnuri Kim ),박지현 ( Ji Hyun Park ),전경미 ( Kyongmi Chon ),박경훈 ( Kyung-hun Park ),문병철 ( Byeong-chul Moon ),백민경 ( Min Kyoung Paik ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

Pyrethroids (PYRs) are a widely used insecticide in agriculture and household area.PYRs caninduce neurotoxic effects in mammalsby disrupting voltage-sensitive sodium channelswhen exposed to overdose.In mammals, PYRs such as deltamethrinis metabolized to 3-phenoxybenzoic acid (3-PBA) in liver that is mainly excreted in urine.Urine is highly useful as a testing matrix because it is easily accessible and can be collected noninvasively. Little studies have reported on the PYRs-dosedependent effects on urinary 3-PBA.This study is designed to identify the correlation of deltamethrinamount exposured orally and dermally to rats and its metabolite(3-PBA) excreted in urine.This study is designed to exposure deltamethrin to rats in a dose-dependent manner and identify the correlation between deltamethrin exposure and its metabolite(3-PBA) in urine. Exposure levels consisted of three concentration of deltamethrin; control (0mg/kgbw), low (0.0705 mg/kgbw), medium (0.705 mg/kgbw) and high(7.05 mg/kgbw) dose. Low concentration of deltamethrin was calculated on the basis of Korea predictive operator exposure model (KoPOEM), indicating the field-realistic exposure to pesticides for farmer when mixing and applying pesticides. Single exposure of deltamethrin to Spraque-Dawley rats were conducted through oral and dermal pathway. Dermal exposure persisted for 6 h, and urine specimens were collected for 24h after exposure of both two pathway. The following procedures including hydrolysis, extraction and derivatization were conducted prior to the analysis of urinary 3-PBA. After acidic hydrolysis for the cleavage of conjugates, the analytes3-PBAwas extracted from urine matrix using n-hexane. Then, 3-PBA was derivatized to volatile estersusing N-tert.-butyldimethylsilyl-N-methyltrifluoroacetamid (MTBSTFA). Separation and detection was carried out using GC/MS. 2-Phenoxybenzoic acid served as internal standard for the quantification of 3-PBA. The limit of quantificationfor all analyteswas 0.98ng/ml urine.In the low-, medium- and high-dose oral deltamethrin groups, the concentrations of urinary 3-PBA were 44±2, 104±37, 943±65 ng/ml urine, respectively, showing good correlation between oral deltamethrin exposure and urinary 3-PBA in rats. This result was similar with those for dermal administration of deltamethrin. Based on these results, therefore, 3-PBA that is a main metabolite of PYRs may be suitable for urinary biomarker of exposure to PYRs.This study can be used as an index that can be predictedto exposuredosage of deltamethrinin the human from detection of urinary 3-PBA excretion.

복원된 양재천에서 복원 효과 평가 및 평가 결과에 기초한 개선방안

김아름,김동욱,임봉순,설재원,이창석,Kim, A Reum,Kim, Dong Uk,Lim, Bong Soon,Seol, Jae Won,Lee, Chang Seok 한국하천호수학회 2020 생태와 환경 Vol.53 No.4

This study was carried out to evaluate the restoration effect in the restored Yangjae stream and to draw up an adaptive management plan based on the results. As the result of evaluation on the restoration effect, the restored Yangjae stream was evaluated with low naturalness in both terms of the morphology of the stream and the composition and spatial distribution of vegetation. The diverse functional groups were introduced in the vegetation restoration, but the flooding regime, which is significant in the spatial distribution of riparian vegetation, were not correctly reflected. Exotic species or species that were not ecologically suitable for the location were introduced on the embankment and thus a measure to improve those problems is required. As the ecological principle was not reflected in the restoration plan, the stream was constructed as the double terrace structure. Therefore, the width of the waterway was narrowed further, and the waterfront was not designed to accommodate changes from flooding disturbance, making the micro-topography of the stream simpler and the naturalness lower. The adaptive management plan was prepared to improve those problems, and a plan for creating an ecological network was recommended to enhance the restoration effect.