폴리에틸렌 글리콜의 공유부착에 의한 Adenosine Deaminase 의 성질변화에 관한 연구

김동규,김은진,강신원,김승원 ( Dong Kyoo Kim,eun Jin Kim,Shin Won Kang,Seung Won Kim ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.1

The adenosine deaminase from calf intestinal mucosa was modified with 2,4-bis(o-methoxy-polyethylene glycol)-6-chloro-s-triazine(activated PEG), which was synthesized from monomethoxypolyethylene glycol(MW 5,000) and cyanuric chloride. Approximately 41 out of the total 69 amino groups in the adenosine deaminase molecule were modified. The conjugation of PEG increased affinity for adenosine showed by decreased K_m. ADA and PEG-ADA had temperature optima of 60℃ and 50℃, respectively. Thermal inactivation at temperature above optima was essentially the same for the native and modified enzyme. PEG-ADA and ADA demonstrated an resistance to proteolytic digestion of trypsin and chymotrypsin. Modification of the adenosine deaminase with PEG resulted in greatly decreased electrophoretic mobility of the protein. The modified ADA preparations were less immunogenic and antigenic than native enzyme as determined by immunodiffusion studies.

선형탄성파괴역학을 이용한 5Ni - Cr - Mo - V 계 고인성 고장력강의 응력부식현상연구

김동규,김규영,권혁상,이성근,심인옥,김영우 ( Dong Kyou Kim,Kyoo Young Kim,Hyuk Sang Kwon,Sung Keun Lee,In Ok Sim,Young Woo Kim ) 한국부식방식학회 1995 Corrosion Science and Technology Vol.24 No.1

수정된 반복 주성분 분석 기법에 대한 연구

김동규,김아현,김현중,Kim, Dong-Gyu,Kim, Ah-Hyoun,Kim, Hyun-Joong 한국통계학회 2011 응용통계연구 Vol.24 No.5

다변량 자료를 분석함에 있어 자료의 차원을 축소하는데 활용되는 중요한 툴 중 하나인 PCA 분석(주성분 분석, Principal Component Analysis)을 실시간으로 처리해야 하는 적용 분야가 최근 늘고 있다. PCA 분석에서는 표본 공분산 행렬의 고유값과 고유벡터를 도출하는 것이 관건인데, 자료의 양이 방대하며 고차원인 경우 이를 실시간으로 수행하기에는 어려움이 따른다. 이러한 문제점을 해결하기 위해서 Erdogmus 등 (2004)는 일차 섭동 이론(first order perturbation theory)을 활용하여 공분산 행렬의 고유값과 고유벡터를 추정하는 Recursive PCA 방법을 제안했다. 이 방법은 추가된 자료의 양이 많지 않은 경우는 상당히 정확하지만, 추가된 자료의 양이 많아짐에 따라 오차도 커진다는 한계를 가지고 있다. 본 논문은 공분산 행렬의 고유값과 고유벡터가 가지고 있는 수학적 관계를 이용하여 Erdogmus 등 (2004)가 제안한 Recursive PCA 방법을 수정한 Modi ed Recursive PCA 방법을 제안하다. 또한, 모의 실험을 통해 Recursive PCA 방법과 Modi ed Recursive PCA 방법에서의 고유값과 고유벡터 추정값의 정확도를 비교해 보았으며 그 결과 기존 Recursive PCA 방법 보다 정확한 추정이 가능함을 확인할 수 있었다. PCA(Principal Component Analysis) is a well-studied statistical technique and an important tool for handling multivariate data. Although many algorithms exist for PCA, most of them are unsuitable for real time applications or high dimensional problems. Since it is desirable to avoid extensive matrix operations in such cases, alternative solutions are required to calculate the eigenvalues and eigenvectors of the sample covariance matrix. Erdogmus et al. (2004) proposed Recursive PCA(RPCA), which is a fast adaptive on-line solution for PCA, based on the first order perturbation theory. It facilitates the real-time implementation of PCA by recursively approximating updated eigenvalues and eigenvectors. However, the performance of the RPCA method becomes questionable as the size of newly-added data increases. In this paper, we modified the RPCA method by taking advantage of the mathematical relation of eigenvalues and eigenvectors of sample covariance matrix. We compared the performance of the proposed algorithm with that of RPCA, and found that the accuracy of the proposed method remarkably improved.

건축편상

김동규,Kim, Dong-Gyu 대한건축사협회 1976 建築士 Vol.1976 No.7

역사속 직업이야기-고려시대에도 타이포그라퍼가 있었다

김동규,Kim, Dong-Gyu 대한인쇄문화협회 2008 프린팅코리아 Vol.69 No.-

소련 고등교육의 방법론과 특성-소련의 고등교육(4)

김동규,Kim, Dong-Gyu 한국대학교육협의회 1989 大學敎育 Vol.42 No.-

굴곡측정법을 이용한 극후판 용접부 잔류응력분포 정량분석

김동규,우완측,강윤희,Kim, Dong-Kyu,Woo, Wanchuck,Kang, Youn-Hee 대한용접접합학회 2015 대한용접·접합학회지 Vol.33 No.1

Residual stresses arising from the materials processing such as welding and joining affect significantly the structural integrity depending on the external loading condition. The quantitative measurement of the residual stresses is of great importance in order to characterize the effects of the residual stresses on the structural safety. In this paper, we introduce a newly devised destructive technique, the contour method (CM), which is applied for the measurements of the residual stress distributions through the thickness of a 80 mm thick steel weld. Residual stresses are evaluated from the contour, which is the normal displacement on a cut surface produced by the relaxation of residual stresses, using a finite element model. The CM provides a two-dimensional map of the residual stresses normal to the cut surface. The CM developed in the present study was validated in comparison with the residual stress distribution determined by a well-established neutron-diffraction residual stress instrument (RSI) instrumented in HANARO neutron research reactor.

Design and Performance Analysis of Electronic Seal Protection Systems Based on AES

김동규,이문규,강유성,정상화,Won-Ju Yoon,Jung-Ki Min,Howon Kim 한국전자통신연구원 2007 ETRI Journal Vol.29 No.6

A very promising application of active RFID systems is the electronic seal, an electronic device to guarantee the authenticity and integrity of freight containers. To provide freight containers with a high level of tamper resistance, the security of electronic seals must be ensured. In this paper, we present the design and implementation of an electronic seal protection system. First, we propose the eSeal Protection Protocol (ePP). Next, we implement and evaluate various cryptographic primitives as building blocks for our protocol. Our experimental results show that AES-CBC-MAC achieves the best performance among various schemes for message authentication and session key derivation. Finally, we implement a new electronic seal system equipped with ePP, and evaluate its performance using a real-world platform. Our evaluation shows that ePP guarantees a sufficient performance over an ARM9- based interrogator.

정수 문자집합상의 접미사트리 구축을 위한 새로운 합병 알고리즘

김동규,심정섭,박근수,Kim, Dong-Kyu,Sim, Jeong-Seop,Park, Kun-Soo 한국정보과학회 2002 정보과학회논문지 : 시스템 및 이론 Vol.29 No.2

주어진 스트링 S의 접미사트리 $T_s$를 구축하기 위하여 , 먼저 홀수위치들에 대한 접미사트리 $ T_0$를 제귀적으로 구축하고 짝수위치들에 대한 접비사트리 $T_e$를 $ T_o$/로 부터 구축한 다음 $ T_o$와 $T_e$를 합병하여 $T_s$를 구축하는 새로운 방식이 사용되고 있다. 인덱스자료구조에 관련된 문제들 중 정수 문자집합상의 접미사트리를 선형시간에 구축하는 문제는 오랫동안 미해결문제로 남아 있었다. Farach은 이 방식을 적용하여 처음으로 성형시간이 소요되는 알고리즘을 제시하였다. 이 알고리즘은 중 가장 어려운 곳은 합병하는 부분이다. 본 논문에서는 BFS(breadth-first search)에 기반하는 새로운 합병알고리즘을 제안한다. 제안된 합병알고리즘은 Farach의 DFS(depth-first search) 방식보다 개념적으로 단순하게 동작하므로 다른 응용의로 쉽게 확장될수 있다. A new approach of constructing a suffix tree $T_s$for the given string S is to construct recursively a suffix tree $ T_0$ for odd positions construct a suffix tree $T_e$ for even positions from $ T_o$ and then merge $ T_o$ and $T_e$ into $T_s$ To construct suffix trees for integer alphabets in linear time had been a major open problem on index data structures. Farach used this approach and gave the first linear-time algorithm for integer alphabets The hardest part of Farachs algorithm is the merging step. In this paper we present a new and simpler merging algorithm based on a coupled BFS (breadth-first search) Our merging algorithm is more intuitive than Farachs coupled DFS (depth-first search ) merging and thus it can be easily extended to other applications.

Enhanced Type 2 Immune Reactions by Increased IL-22/IL-22Ra1 Signaling in Chronic Rhinosinusitis With Nasal Polyps

김동규,Ara Jo,Hee-Suk Lim,김진엽,Kyoung Mi Eun,Jayoung Oh,Joon Kon Kim,Seong-Ho Cho,김대우 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.6

Purpose: Recent studies have revealed the pathogenic role of interleukin (IL)-22 in atopic dermatitis and asthma. However, little is known about the role of IL-22 in the pathophysiology of chronic rhinosinusitis with nasal polyps. We aimed to investigate the expression of IL-22 and its pathogenic function in type 2 immune reactions of nasal polyps (NP). Methods: Protein levels of inflammatory mediators were determined by multiplex immunoassay, and principal component analysis (PCA) was performed. Immunofluorescence analysis and mast cell culture were used to determine the cellular sources of IL-22. Normal human bronchial epithelial (NHBE) cells were stimulated using IL-22 in combination with diverse cytokines, and thymic stromal lymphopoietin (TSLP) was measured. Results: IL-22 expression was not up-regulated in NP compared with control tissues, but IL-22-high NP revealed distinct features characterized by type 2 inflammatory cytokines such as chemokine (C-C motif) ligand (CCL)-11, CCL-24, and IL-5 on the PCA. Additionally, IL-22 positively correlated with type 2 immune mediators and the disease severity in NP. For the localization of the cellular sources of IL-22 in eosinophilic NP, it was expressed in cells mostly composed of eosinophil peroxidase-positive cells and partially of tryptase-positive cells. The human mast cell line, LAD2 cells, released IL-22 mediated by immunoglobulin E. Moreover, IL-22 receptor subunit alpha-1 (IL-22Ra1) expression was significantly increased in NP. IL-22Ra1 was up-regulated with poly(I:C) stimulation in NHBE cells. Furthermore, TSLP production was enhanced when stimulated with a combination of IL-13, poly(I:C), and IL-22. Treatment with anti-IL-22Ra1 also inhibited IL-22-induced enhancement of TSLP production. Conclusion: IL-22 was associated with type 2 inflammatory reactions in NP. The IL-22/IL-22Ra1 axis was enhanced and might be involved in type 2 inflammatory reactions via TSLP production in NP.