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      • SCIESCOPUSKCI등재

        폴리에틸렌 글리콜의 공유부착에 의한 Adenosine Deaminase 의 성질변화에 관한 연구

        김동규,김은진,강신원,김승원 ( Dong Kyoo Kim,eun Jin Kim,Shin Won Kang,Seung Won Kim ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.1

        The adenosine deaminase from calf intestinal mucosa was modified with 2,4-bis(o-methoxy-polyethylene glycol)-6-chloro-s-triazine(activated PEG), which was synthesized from monomethoxypolyethylene glycol(MW 5,000) and cyanuric chloride. Approximately 41 out of the total 69 amino groups in the adenosine deaminase molecule were modified. The conjugation of PEG increased affinity for adenosine showed by decreased K_m. ADA and PEG-ADA had temperature optima of 60℃ and 50℃, respectively. Thermal inactivation at temperature above optima was essentially the same for the native and modified enzyme. PEG-ADA and ADA demonstrated an resistance to proteolytic digestion of trypsin and chymotrypsin. Modification of the adenosine deaminase with PEG resulted in greatly decreased electrophoretic mobility of the protein. The modified ADA preparations were less immunogenic and antigenic than native enzyme as determined by immunodiffusion studies.

      • KCI등재

        수정된 반복 주성분 분석 기법에 대한 연구

        김동규,김아현,김현중,Kim, Dong-Gyu,Kim, Ah-Hyoun,Kim, Hyun-Joong 한국통계학회 2011 응용통계연구 Vol.24 No.5

        다변량 자료를 분석함에 있어 자료의 차원을 축소하는데 활용되는 중요한 툴 중 하나인 PCA 분석(주성분 분석, Principal Component Analysis)을 실시간으로 처리해야 하는 적용 분야가 최근 늘고 있다. PCA 분석에서는 표본 공분산 행렬의 고유값과 고유벡터를 도출하는 것이 관건인데, 자료의 양이 방대하며 고차원인 경우 이를 실시간으로 수행하기에는 어려움이 따른다. 이러한 문제점을 해결하기 위해서 Erdogmus 등 (2004)는 일차 섭동 이론(first order perturbation theory)을 활용하여 공분산 행렬의 고유값과 고유벡터를 추정하는 Recursive PCA 방법을 제안했다. 이 방법은 추가된 자료의 양이 많지 않은 경우는 상당히 정확하지만, 추가된 자료의 양이 많아짐에 따라 오차도 커진다는 한계를 가지고 있다. 본 논문은 공분산 행렬의 고유값과 고유벡터가 가지고 있는 수학적 관계를 이용하여 Erdogmus 등 (2004)가 제안한 Recursive PCA 방법을 수정한 Modi ed Recursive PCA 방법을 제안하다. 또한, 모의 실험을 통해 Recursive PCA 방법과 Modi ed Recursive PCA 방법에서의 고유값과 고유벡터 추정값의 정확도를 비교해 보았으며 그 결과 기존 Recursive PCA 방법 보다 정확한 추정이 가능함을 확인할 수 있었다. PCA(Principal Component Analysis) is a well-studied statistical technique and an important tool for handling multivariate data. Although many algorithms exist for PCA, most of them are unsuitable for real time applications or high dimensional problems. Since it is desirable to avoid extensive matrix operations in such cases, alternative solutions are required to calculate the eigenvalues and eigenvectors of the sample covariance matrix. Erdogmus et al. (2004) proposed Recursive PCA(RPCA), which is a fast adaptive on-line solution for PCA, based on the first order perturbation theory. It facilitates the real-time implementation of PCA by recursively approximating updated eigenvalues and eigenvectors. However, the performance of the RPCA method becomes questionable as the size of newly-added data increases. In this paper, we modified the RPCA method by taking advantage of the mathematical relation of eigenvalues and eigenvectors of sample covariance matrix. We compared the performance of the proposed algorithm with that of RPCA, and found that the accuracy of the proposed method remarkably improved.

      • KCI등재
      • KCI등재후보

        극기 훈련 후 발생한 비정형 발살바 망막병증

        김동규,이우진,조문식,김준순.Dong-Kyu Kim. M.D.. Woo-Jin Lee. M.D.. Moon-Sik Cho. M.D.. Joon-Soon Kim. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.5

        Purpose: To report a case of recurrent bilateral preretinal hemorrhages in a healthy male with no previous history of systemic or ocular diseases and to review the relevant literature. Methods: History taking, measurement of visual acuity and intraocular pressure, slit-lamp examination, fundoscopy, and fluorescein angiography were performed in a 15-year-old male who had experienced a sudden decrease in visual acuity in both eyes after a couple of strenuous exercises in school. Results: Best-corrected visual acuity (BCVA) was 0.9 in the right eye and 0.2 in the left, and bilateral premacular hemorrhages were observed. Most of the preretinal hemorrhages were completely resolved by the sixth week and visual acuity in the left eye improved to 0.6. Nine months after the initial event, preretinal hemorrhages recurred bilaterally, worse in the left eye, and BCVA decreased to 0.3 in the right eye and 0.02 in the left eye. We performed Nd:YAG laser disruption of the internal limiting membrane in the left eye. Four weeks after the procedure, most of the preretinal hemorrhages were resolved and visual acuity in the left eye improved to 0.2. Conclusions: Clinical improvement was achieved after Nd:YAG laser disruption of the internal limiting membrane in a case of recurrent bilateral preretinal hemorrhages.

      • KCI등재

        운영중인 지하구조물 누수처리를 위한 유도배수공법 개발 및 성능 검증

        김동규,임민진,Kim, Dong-Gyou,Yim, Min-Jin 한국터널지하공간학회 2017 한국터널지하공간학회논문집 Vol.19 No.3

        본 연구에서는 지하 콘크리트 구조물 내로 누수된 지하수를 유도하여 배수하는 유도배수공법을 제시하였다. 유도배수공법은 유도배수판, 철망, 고정핀, 광물질 혼입 모르타르로 구성된다. 시공성 향상을 위하여 유도배수판 및 철망은 공압타카와 고정핀를 사용하여 콘크리트 표면에 부착하였다. 고로슬래그미분말을 30% 혼입한 모르타르는 뿜어 붙임 모르타르 장비를 사용하여 유도배수판 및 철망 표면에 타설하였다. 유도배수공법의 현장 시공성 및 성능 검증을 위하여 재래식 콘크리트라이닝 터널과 콘크리트 옹벽에서 시험시공을 수행하였다. 시험시공 완료 3년 후 유도배수공법은 성능저하의 문제점이 없는 것으로 판단되었다. 재령 14일과 3년에 뿜어 붙임 모르타르의 부착력 실험을 수행하였다. 유도배수판 표면에 철망을 적용한 경우 뿜어 붙인 모르타르의 부착력은 재령 14일에 1.04 MPa, 재령 3년에 1.46 MPa로 측정되었다. 철망을 적용하지 않은 경우의 부착력은 재령 14일에 1.13 MPa로 측정되었지만, 재령 3년에는 0.89 MPa로 기준 부착력 1 MPa보다 낮게 측정되었다. In this study, drainage systems were proposed to drain the leakage of groundwater in the existing underground concrete structures. The system consists of drainage board, wire mesh, fixed nail, and mortar with mineral. In order to increase constructability, the drainage board and wire mesh were attached on the surface of cement concrete using the air nailer and fixed nail. The mortar with 30% of blast furnace slag was sprayed on the drainage board and wire mesh using the spray mortar equipment. The field test construction was carried out in a conventional concrete lining tunnel and concrete retaining wall for performance verification of the drainage system in the field. There was no problem with performance degradation in the drainage system for three years after construction. The bond strength tests were performed on the sprayed mortar at 14 days and about 3 years after field test construction. In case of attaching the wire mesh on the drainage board, the bond strengths of the sprayed mortar were 1.04 MPa at 14 days and 1.46 MPa about 3 years. In case of the drainage board without the wire mesh, the bond strengths of the sprayed mortar were 1.13 MPa at 14 days and 0.89 MPa, less than 1 MPa of bond strength criteria, about 3 years.

      • KCI등재

        Design and Performance Analysis of Electronic Seal Protection Systems Based on AES

        김동규,이문규,강유성,정상화,Won-Ju Yoon,Jung-Ki Min,Howon Kim 한국전자통신연구원 2007 ETRI Journal Vol.29 No.6

        A very promising application of active RFID systems is the electronic seal, an electronic device to guarantee the authenticity and integrity of freight containers. To provide freight containers with a high level of tamper resistance, the security of electronic seals must be ensured. In this paper, we present the design and implementation of an electronic seal protection system. First, we propose the eSeal Protection Protocol (ePP). Next, we implement and evaluate various cryptographic primitives as building blocks for our protocol. Our experimental results show that AES-CBC-MAC achieves the best performance among various schemes for message authentication and session key derivation. Finally, we implement a new electronic seal system equipped with ePP, and evaluate its performance using a real-world platform. Our evaluation shows that ePP guarantees a sufficient performance over an ARM9- based interrogator.

      • KCI등재

        부분구조합성법을 이용한 컴프레서 쉘의 동특성 규명 및 개선에 관한 연구

        김동규,김종배,고상철,한광희,오재응,Kim, Dong-Kyu,Kim, Jong-Bae,Go, Sang-Chul,Han, Kwang-Hee,Oh, Jae-Eung 한국음향학회 1996 韓國音響學會誌 Vol.15 No.5

        컴프레서의 소음은 냉장고로부터 발생하는 전체 소음레벨중 높은 기여를 나타낸다. 그리고, 컴프레서로부터 방사되는 소음의 상당부분이 컴프레서 쉘의 진동에 의해 방사되는 소음이다. 본 연구에서는 컴프레서 쉘의 동특성을 규명하기 위하여 범용 구조해석 패키지인 MSC/NASTRAN을 이용하여 컴프레서 쉘을 여러 분계로 분리하여 해석한 뒤, 부분구조 합성법을 이용하여 전계의 동특성을 규명하였다. 그리고 각 분계의 변형 에너지와 운동에너지를 산정하여 각 분계가 전계에 기여하는 정도를 파악하였다. 컴프레서 소음에 가장 높은 영향을 미치는 1차모드를 고주파로 이동시키기 위하여, 1차 모드에 기여가 높은 분계의 질량과 강성을 변경하여 컴프레서 쉘의 동특성 개선 방향을 제시하였다. The noise of a compressor is a major contributor to overall noise radiated from the refrigerator. The major source of the noise is radiated by the vibration of the compressor shell. In this study, to identify the dynamic characteristics of compressor shell, a compressor shell is divided into several components and these are analyzed with a commercial FEM(Finite Element Method) package such as MSC/NASTRAN. Using substructure synthesis method, the dynamic characteristics of the total system is identified. The coherence of each component to the total system is computed by using strain and kinetic energy. To increase the frequency of the first resonance mode which is most effective mode to the noise of the compressor shell, the improving strategy of dynamic characteristics is suggested by changing mass and stiffness of the coherence component to the first mode.

      • KCI등재후보

        동적 자동시야계를 이용한 양안 복시시야검사의 유용성

        김동규,최경식,박성희,Dong-Kyu Kim,Kyung-Seek Choi,Song-Hee Park 대한안과학회 2005 대한안과학회지 Vol.46 No.7

        Purpose: To compare the binocular double vision field for diplopia with Goldmann perimetry and Humphrey automated kinetic perimeter. Methods: Fifty of 70 healthy volunteers with no ocular problem wore the glasses of 14 prism diopters(PD)(7 prism lenses on both eyes, base-in), and 20 of the 70 wore the glasses of 20 PD(10 prism lenses on both eyes, base-out) and of 5 PD(only on right eye, base-up), and then esotropia, exotropia and hypertropia were induced in each group, respectively. Humphrey automated kinetic perimetry and Goldmann perimetry were performed simultaneously and their results were analyzed using mean degrees of meridians and binocular single vision(BSV) score. Results: Diplopia fields of Humphrey automated kinetic perimetry and Goldmann perimetry showed a similar overlap. Mean degree of meridians of Goldmann perimetry and Humphrey automated kinetic perimetry were 34.49/30.26, 32.08/28.31 and 31.93/27.09 degrees at esotropia, exotropia and hypertropia, respectively. BSV scores were 80.43/73.70, 74.34/67.13 and 67.16/62.13%, respectively. Diplopia field areas of Humphrey automated kinetic perimetry were significant larger than those of Goldmann (p<0.05). Conclusions: This study shows that if results of Humphrey automated kinetic perimetry are corrected by means of multiplying by 1.14, diplopia tests using Humphrey automated kinetic perimetry may be substituted for Goldmann perimetry.

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