Delayed Clinical Signs Associated with Cerebellar Lesion in Holstein Calf Infected with Bovine Viral Diarrhea Virus

김단일 한국임상수의학회 2022 한국임상수의학회지 Vol.39 No.1

A 5-month-old Holstein downer calf was presented, and bovine viral diarrhea virus was detected in the feces and the cerebrospinal fluid. Combined treatment of recombinant bovine somatotropin (rBST) administration and standing rehabilitation had been performed for two months, and the calf could maintain the standing position for a while. However, the symptoms were not improved, and the calf died due to ruminal tympany and aspiration pneumonia. Megaesophagus, abomasal ulcer, and a normal-sized but softened cerebellum were observed in clinical necropsy. Improvement of symptoms was confirmed by rBST, but was not reached the complete recovery of gait and standing position

Surgical Correction of Bilateral Gastrocnemius Muscle Rupture and Its Prognosis in a Korean Native Calf

김단일 한국임상수의학회 2023 한국임상수의학회지 Vol.40 No.3

A 3-month-old Korean native cattle (Hanwoo) calf with difficulty tak- ing normal posture and an inability to rise was referred for a definite diagnosis and active treatment, including surgery. The calf had a history of an accident in which both hind limbs were trapped in a barn structure. After admission, a “rabbit leg” posture was observed, a typical sign of gastrocnemius muscle rupture, and both digits were knuckled downward like they were trying to grip the ground. This was considered to be a result of the superficial digital flexor not rupturing but only the gastrocnemius muscle rupturing. Physical examination revealed lac- eration of the metatarsus and firmness behind both stifle joints which were pre- sumed to be the sites of gastrocnemius muscle rupture. Skeletal abnormalities, including fractures, were ruled out by radiography. Based on these findings, the patient was diagnosed with bilateral gastrocnemius muscle rupture, and surgery was performed to reconnect the head of the ruptured muscle. Because the rup- ture occurred perpendicular to the muscle direction, the locking loop technique, a method of suturing severed tendons, was used to reduce the tension. After surgery, the cast was used to prevent further injuries and promote voluntary rehabilitation. Follow-up was completed, with the calf showing normal posture and gait 112 days after surgery. This is the first case report in the Republic of Ko- rea describing the successful diagnosis and treatment of bilateral gastrocnemius muscle rupture in a calf.

17세기 『大方廣佛華嚴經疏』의 간행과 승려문파 - 삭녕 용복사본과 순천 송광사본을 중심으로 -

김단일 한국서지학회 2020 서지학연구 Vol.83 No.-

이 연구는 17세기에 간행된 삭녕 용복사본과 순천 송광사본 『대방광불화엄경소(大方廣佛華嚴經疏)』의 간행 배경을 살펴본 연구이다. 『대방광불화엄경소』는 정원(淨源, 1011-1088)이 『대방광불화엄경(大方廣佛華嚴經)』과 징관(澄觀, 738-839)의 주석을 회편한 120권 분량의 불서이다. 고려시대 전래된 이래로 17세기까지 여러 차례 간행되었는데 특히 17세기에는 삭녕 용복사와 순천 송광사에서 2년 차이로 간행되었다. 삭녕 용복사본과 순천 송광사본 『대방광불화엄경소』는 형태적으로는 계선의 차이가 있지만, 원문 내용에서는 이전 시기의 『대방광불화엄경소』와 크게 다르지 않았다. 그러나 두 판본의 간행 관련 기록을 살펴보면 간행 참여자들이 각각 삭녕 용복사본은 청허문파, 순천 송광사본은 부휴문파와 관련이 있음을 알 수 있다. 이를 통해서 17세기 『대방광불화엄경소』 간행에는 승려문파라는 요소가 크게 영향을 미쳤음을 알 수 있다. This paper presents an investigation into the background of Daebanggwangbul-hwaeomgyeongso, published at Yongboksa Temple in Saknyeong and Songgwangsa Temple in Suncheon in the 17th century. Daebanggwangbul-hwaeomgyeongso is a collection of 120 Buddhist books based on the collection and editing of Avatamsaka Sutra and the annotations of chéngguān (澄觀, 738-839) by jìngyuán (淨源, 1011-1088). Since its introduction during Goryeo, it was published many times into the 17th century. Especially in 17th century, It was published at Yongboksa Temple in Saknyeong and Songgwangsa Temple in Suncheon with a two-year interval between them. The editions of Daebanggwangbul-hwaeomgyeongso published at Yongboksa Temple in Saknyeong and Songgwangsa Temple in Suncheon are different forms as each line has an end mark. However, their content is not that different from the original content of the Daebanggwangbul-hwaeomgyeongso editions published before. A closer look at the records about the publication of the two editions shows that the participants in publishing the edition at Yongboksa Temple in Saknyeong and Songgwangsa Temple in Suncheon were associated with the Cheongheo and Buhyu Clan, respectively. These findings indicate that the monk clans had huge impacts on the publication of Daebanggwangbul-hwaeomgyeongso in the 17th century.

젖소 유방염에서 16S rRNA 파이로시퀀싱을 이용한 우유 내 마이크로바이옴의 동정과 난백의 항균효과

김단일,김은경,성원진,노영혜,고대성,김남형,김재홍,권혁준,Kim, Danil,Kim, Eun-Kyung,Seong, Won-Jin,Ro, Younghye,Ko, Dae-Sung,Kim, Nam-Hyung,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2017 大韓獸醫學會誌 Vol.57 No.2

Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.

설명 문장 생성을 통한 해석 가능한 시각적 질의응답 모델 분석

김단일(Danil Kim),한보형(Bohyung Han) 한국컴퓨터정보학회 2020 한국컴퓨터정보학회 학술발표논문집 Vol.28 No.2

본 연구에서는 설명 문장 생성을 통한 해석 가능한 시각적 질의응답 모델을 설계하고 학습 방법을 제시한다. 설명 문장은 시각적 질의응답 모델이 응답을 예측하는 데에 필요한 이미지 및 질문 정보와 적절한 논리적인 정보의 조합 및 정답 추론 과정이 함의되어 있을 것으로 기대한다. 설명 문장 생성 과정이 포함된 시각적 질의응답의 기본적인 모델을 기반으로 여러 가지 학습방법을 통해 설명 문장 생성 과정과 응답 예측 과정간의 상호관계를 분석한다. 이러한 상호작용을 적극적으로 활용할 수 있는 보다 개선 시각적 질의응답 모델을 제안한다. 또한 학습한 결과를 바탕으로 설명 문장의 특성을 활용하여 시각적 질의응답 추론 과정을 개선함으로써 시각적 질의응답 모델의 발전 방향을 논의한다. 본 실험을 통해서 응답 예측에 적절한 설명 문장을 제시하는 해석 가능한 시각적 질의응답 모델을 제공한다.

rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정

성원진,김단일,김은경,고대성,노영혜,김재홍,권혁준,Seong, Won-Jin,Kim, Danil,Kim, Eun-Kyung,Ko, Dae-Sung,Ro, Younghye,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2018 大韓獸醫學會誌 Vol.58 No.1

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.

젖소 목장의 유두침지소독 방법에 따른 집합유와 시판 우유 내 요오드 농도

정지혁,김단일,Doo Kim 한국임상수의학회 2016 한국임상수의학회지 Vol.33 No.5

To determine the concentrations of commercial milks and the effect of different teat dipping methods oniodine concentrations of bulk tank milk, iodine concentration was analyzed by inductively coupled plasma massspectrometry (ICP-MS). The 59 bulk tank milk samples were collected. The 8 brands of commercial whole milk and5 brands of commercial organic milk were purchased. The iodine concentrations of bulk tank milks of 59 dairy farmswere in the range from 42.7 to 562.5 μg/L. The iodine levels of 4 different teat dipping methods with pre-dipping,post-dipping, pre & post-dipping, and non-dipping were 138.9 ± 41.1, 200.6 ± 106.9, 205.1 ± 93.2, and 110.9 ± 70.4 μg/L, respectively. No significant difference (P > 0.05) was observed among 4 different teat dipping methods. Iodineconcentrations of commercial whole milks were in the range from 149.1 to 210.4 (178.8 ± 22.0) μg/L, and iodine concentrationsof commercial organic milks were in the range from 85.0 to 356.9 (214.2 ± 123.3) μg/L. Iodine concentrationsof commercial milk in Korea are relatively low comparing with those of other countries

Introduction of the Laboratory Animal Center at DGMIF

최우성,한대용,김단일,김동규,박준석,김성곤,김충용 한국실험동물학회 2012 한국실험동물학회 학술발표대회 논문집 Vol.2012 No.8

Diagnosis of Laryngeal Cyst using Respiratory Endoscopy in Hanwoo Cattle with Chronic Bronchopneumonia

노영혜,최우재,Hoyung Kim,김단일 한국임상수의학회 2018 한국임상수의학회지 Vol.35 No.2

A seven-month-old Hanwoo steer was presented immediately after transport with respiratory symptomsincluding a nasal discharge, depression, and anorexia. Though repeated treatments, bronchopneumonia had not beenimproved and had persisted for 10 months. Then, obstructive breath sound was heard. A cyst adjacent to the epiglottiscould be observed with respiratory endoscopy. Consequently, chronic bronchopneumonia induced laryngeal cystformation, resulting in obstructive dyspnea. And respiratory endoscopy may be useful for differentiating the causesof dyspnea in bovine clinical practice.

Prepartal Decrease in Plasma Total Cholesterol Concentration in Dairy Cows Developed Subclinical Ketosis

노영혜,최우재,김호영,김단일 한국임상수의학회 2017 한국임상수의학회지 Vol.34 No.3

A retrospective study was designed to determine whether there were any differences in prepartal blood biochemical parameters between cows developed subclinical ketosis (SCK) and non-SCK cows. Data on blood biochemistry from 33 Holstein parturient cows (15 SCK and 18 non-SCK cows) in one farm for one year, were compared. Plasma beta-hydroxybutyrate, non-esterified fatty acid and glucose concentrations were utilized in the diagnosis of SCK and showed significant differences between SCK and non-SCK cows after calving. Before calving, however, only plasma total cholesterol concentration in SCK ows was significantly lower than that in non-SCK cows. Consequently, it seems that lower plasma total cholesterol concentration before calving is related o the occurrence of SCK immediately after calving.