Prepartal Decrease in Plasma Total Cholesterol Concentration in Dairy Cows Developed Subclinical Ketosis

노영혜,최우재,김호영,김단일 한국임상수의학회 2017 한국임상수의학회지 Vol.34 No.3

A retrospective study was designed to determine whether there were any differences in prepartal blood biochemical parameters between cows developed subclinical ketosis (SCK) and non-SCK cows. Data on blood biochemistry from 33 Holstein parturient cows (15 SCK and 18 non-SCK cows) in one farm for one year, were compared. Plasma beta-hydroxybutyrate, non-esterified fatty acid and glucose concentrations were utilized in the diagnosis of SCK and showed significant differences between SCK and non-SCK cows after calving. Before calving, however, only plasma total cholesterol concentration in SCK ows was significantly lower than that in non-SCK cows. Consequently, it seems that lower plasma total cholesterol concentration before calving is related o the occurrence of SCK immediately after calving.

Detection of Fever with Subcutaneously Implanted Thermo-Loggers in Cattle Administered with Lipopolysaccharide

노영혜,복진덕,이헌준,강상기,김단일,이윤석 한국임상수의학회 2018 한국임상수의학회지 Vol.35 No.3

The aim of this study is to determine whether subcutaneous temperature (ST) was correlated with rectaltemperature (RT) in cattle with inducing artificial fever. In order to determine the correlation between their temperatures,the experiment was performed as follow: Among nine Holstein steers, lipopolysaccharide (LPS) was intravenouslyadministered at a dose of 0.5 μg/kg of body weight to six Holstein steer, then, 6 ml of saline was administrated tothree steers as a control group. After LPS injection, ST was recorded using subcutaneously implanted thermo-loggersensors at 10-min intervals, and RT was measured using a digital thermometer at 0, 1, 2, 3, 4, 8 and 12 h. In steerswith LPS injection, RT was highest at 3 to 4 h and recovered to a pre-challenge temperature at 8-22 h. A similarfluctuation was shown in ST except for an unexpected decrease at 1 h, and a positive correlation between RT andST was observed in LPS-challenged steers (r = 0.497, P = 0.04). This result suggests that ST could be utilized as anindex for early detection of infectious diseases or physiological events.

다문화가족 방문교육지도사의 역할적응 과정: 근거이론적 접근

노영혜,김원중 한국복지상담교육학회 2019 복지상담교육연구 Vol.8 No.2

본 연구는 다문화가족 방문교육지도사의 역할적응 과정을 심층적이고 포괄적으로 파악하여 이 과정에 관계되는 조건 및 도달하게 되는 결과를 설명할 수 있는 실체이론을 구축하기 위한 목적으로 수행되었으며, 연구의 목적을 달성하기 위해 Strauss와 Corbin(1998)의 근거이론 방법론을 적용하였다. 연구의 참여자는 5년 이상 경력이 있는 다문화가족 방문교육지도사 11명으로 모두 여성이며, 연구 참여자 선정은 근거이론에서 연구자가 이론을 가장 잘 형성하도록 돕기 위해 이론적으로 선택하는 이론적 표본추출(Strauss & Corbin, 1998)을 사용하였다. 자료 수집은 2017년 3월 21일부터 2018년 9월까지 총 18개월에 동안 이루어졌다. 자료 분석은 Strauss와 Corbin(1998)의 근거이론 방법에 따라 개방 코딩, 축 코딩, 패러다임에 의한 범주분석과 과정 분석을 실시하였다. 선택 코딩에서 핵심범주를 도출하고 이야기 개요를 작성한 후 핵심범주를 중심으로 역할적응 과정과 유형화를 하였다. 연구 결과, 개방 코딩에서는 총 118개의 개념과 33개의 하위범주, 13개의 범주가 도출되었다. 연구 결과를 요약하면 다음과 같다. 첫째, 다문화가족 방문교육지도사의 역할적응 과정의 핵심범주는 ‘주체적인 방문교육지도사로 거듭나기’로 나타났다. 둘째, 축 코딩에서 패러다임에 의한 범주분석 결과, 다문화가족 방문교육지도사 역할적응 과정의 인과적 조건으로는 ‘일에 대한 의욕’, ‘다문화가정에 대한 이해 부족’으로 나타났고, 중심현상은 ‘역할에 대한 혼란 및 갈등’으로 나타났다. 맥락적 조건은 ‘심리적 탈진’으로 나타났고, 중재적 조건은 ‘동력요인’, ‘장애요인’으로 나타났다. 작용/상호작용 전략은 ‘자기성찰과 자기돌봄’, ‘자기개발을 통한 맞춤식 서비스’, ‘자원연계 및 정보제공’으로 나타났고, 결과는 ‘역할 정체성 확립 및 심리적 안정’, ‘성장 및 변화’, ‘전문성에 대한 지속적 추구’로 나타났다. 셋째, 다문화가족 방문교육지도사의 역할적응 과정은 ‘혼란과 갈등’, ‘심리적 탈진’, ‘자기관리 및 자기개발’, ‘성장과 변화’, ‘전문성에 대한 지속적 추구’ 5가지 단계로 도출되었다. 넷째, 다문화가족 방문교육지도사의 역할적응 유형은 ‘주도형’과 ‘합의형’ 그리고 ‘맞춤형’ 3가지로 분류되었다. This study was conducted to identify the role adaptation process of multi-cultural family home visiting educators and establish the substance theory that could be used to explain the conditions relevant in this process and the results that could be reached, and to achieve the purpose of study, Strauss and Corbin(1998)’s grounded theory methodology was applied. Participants in this study were 11 women who were multi-cultural family home visiting educators with more than five years of career, and for research participant selection, this study used theoretical sampling (Strauss & Corbin, 1998), which is theoretically selected in grounded theory to help researcher form a theory the best. Data collection was proceeded for 18 months in total from Mar. 21, 2017 to Sept. 2018. Data analysis was conducted with the category analysis that was based on open coding, axial coding, and paradigm and the process analysis, according to Strauss and Corbin (1998)’s grounded theory methodology. Core category was drawn from selective coding and story outline was written and then centering on core category, role adaptation process classification was conducted. As a result of study, a total of 118 concepts and 33 subcategories and 13 categories were drawn from open coding. Study results are summarized as follows: First, core category in role adaptation process of multi-family home visiting educators appeared as ‘reborn as an independent home visiting educator.’ Second, as a result of category analysis with paradigm in axial coding, causal conditions in role adaptation process of multi-cultural family home visiting educators appeared as ‘enthusiasm for work’ and ‘lack of understanding of multi-cultural family’ and central phenomenon appeared as ‘confusion and conflict against role.’ Contextual condition appeared as ‘psychological burnout’ and intervening condition appeared as ‘driving factor’ and ‘obstructive factor.’ Action/interaction strategy appeared as ‘self-examination and self-care’, ‘customized service through self development’, and ‘resource linkage and information provision’ and results appeared as ‘role identity establishment and psychological stability’, ‘growth and change’, and ‘constant pursuit of professionalism.’ Third, the role adaptation process of multi-cultural family home visiting educators was drawn into the following five stages: ‘confusion and conflict’, ‘psychological burnout’, ‘self management and self development’, ‘growth and change’, and ‘constant pursuit of professionalism.’ Fourth, the role adaptation type of multi-cultural family home visiting educators was classified into the following three categories: ‘leading type’, ‘settlement type’ and ‘customizing type.’

Diagnosis of Laryngeal Cyst using Respiratory Endoscopy in Hanwoo Cattle with Chronic Bronchopneumonia

노영혜,최우재,Hoyung Kim,김단일 한국임상수의학회 2018 한국임상수의학회지 Vol.35 No.2

A seven-month-old Hanwoo steer was presented immediately after transport with respiratory symptomsincluding a nasal discharge, depression, and anorexia. Though repeated treatments, bronchopneumonia had not beenimproved and had persisted for 10 months. Then, obstructive breath sound was heard. A cyst adjacent to the epiglottiscould be observed with respiratory endoscopy. Consequently, chronic bronchopneumonia induced laryngeal cystformation, resulting in obstructive dyspnea. And respiratory endoscopy may be useful for differentiating the causesof dyspnea in bovine clinical practice.

접촉각과 관능 평가의 매칭을 통한 지속성 측정 연구

장순희,노영혜,한은실,이지명,이지영,심민경,최영진 한국감성과학회 2011 추계학술대회 Vol.2012 No.-

Hemorrhagic Enteritis in Two One-month-old Dairy Calves Infected with Beta2-toxigenic Clostridium perfringens and Coccidium

최우재,노영혜,김호영,김은경,최은희,김단일 한국임상수의학회 2017 한국임상수의학회지 Vol.34 No.2

Two one-month-old dairy calves which have Eimeria oocysts in their bloody diarrhea died acutely a few days after showing the first clinical signs. At necropsy, hemorrhagic and congestive gastrointestinal organs were observed in both calves, and abomasal ulcerations existed. As a prevalent agent in all of the collected intra-intestinal specimens, Clostridium perfringens was isolated and the strain was identified as type A possessing alpha and beta2-toxins. In these clinical cases, intercurrent infection by C. perfringens type A and Eimeria through contaminated environment may be responsible for acute hemorrhagic enteritis.

젖소 유방염에서 16S rRNA 파이로시퀀싱을 이용한 우유 내 마이크로바이옴의 동정과 난백의 항균효과

김단일,김은경,성원진,노영혜,고대성,김남형,김재홍,권혁준,Kim, Danil,Kim, Eun-Kyung,Seong, Won-Jin,Ro, Younghye,Ko, Dae-Sung,Kim, Nam-Hyung,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2017 大韓獸醫學會誌 Vol.57 No.2

Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.

점증폴리머의 레올로지 특성이 마스카라 물성에 미치는 영향

최선경 ( Sun Kyung Choi ),노영혜 ( Young Hea Roh ),추정한 ( Jeong Han Choo ),최영진 ( Yeong Jin Choi ),강학희 ( Hak Hee Kang ),이옥섭 ( Ock Sub Lee ) 대한화장품학회 2007 대한화장품학회지 Vol.33 No.3

본 연구에서는 O/W 제형 마스카라에서의 레올로지 특성과 함께, 마스카라 내의 수상 폴리머에 따른 발림성과 볼륨과의 상관관계를 살펴보고자 하였다. 마스카라의 레올로지 특성은 최종 마스카라 내용물과 점증 수용액으로 측정하였으며, 수상 점증 폴리머는 hydroxyethylcellulose (HC), carboxymethylcellulose (CMC), hectorite, sodium magnesium silicate (SMS), hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer (HS), polyacrylate 13/polyisobutene/polysorbate 20 (PPP)를 사용하였다. 실험결과 2.0 wt%의 수용액 점도에서 아크릴계 점증 폴리머인 HS와 PPP가 가장 높은 부착력을 가졌으며, 이는 마스카라의 품질 특성 중 볼륨과 밀접한 관계를 가졌다. 또한 HC와 SMS, HS를 0.2 wt% 함유한 마스카라를 oscillation stress가 1.0 ∼ 1,000 s^-1의 범위에서 저장계수를 측정한 결과 높은 진동힘 구간인 100 ∼ 1,000 s^-1 구간에서 SMS의 저장계수가 가장 낮게 측정되었고, 이는 마스카라 특성 중 발림성 항목과 밀접한 관계를 가졌다. 이는 O/W제형의 마스카라에서 수상 점증제의 구조적인 특성이 마스카라 물성에 크게 영향을 주어 최종적으로 볼륨과 발림성 등의 사용감에도 큰 영향을 주고 있음으로 해석할 수 있다. The relationship between texture and rheological properties of mascara (oil-in-water emulsion) was analyzed in this study. The final mascara product and gelling agents (2.0 wt%) used therein, such as hydroxyethylcellulose (HC), carboxymethylcellulose (CMC), hectorite, sodium magnesium silicate (SMS), hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer (HS), and polyacrylate 13/polyisobutene/polysorbate 20 (PPP), were measured for rheological properties. As a result, HS and PPP showed the highest adhesiveness which were related to the volumizing effect of mascara. The viscosities of HC, SMS, and HS were measured at the stress range of 1 ∼ 1,000 s^-1. SMS, with the lowest storage modulus range of 100 ∼ 1,000 s^-1, affected the mascara in terms of smooth texture. The results of this study suggest that the rheology of gelling agents used influences the final texture of the mascara.

rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정

성원진,김단일,김은경,고대성,노영혜,김재홍,권혁준,Seong, Won-Jin,Kim, Danil,Kim, Eun-Kyung,Ko, Dae-Sung,Ro, Younghye,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2018 大韓獸醫學會誌 Vol.58 No.1

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.

소 결핵 진단을 위한 인터페론감마 검사 키트의 성능 비교 평가

홍이곤 ( Leegon Hong ),최우재 ( Woojae Choi ),노영혜 ( Younghye Ro ),안선민 ( Sunmin Ahn ),김은경 ( Eunkyung Kim ),최은희 ( Eunhee Choe ),김단일 ( Danil Kim ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.4

In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon- gamma (IFN-γ) is included in Korea’s diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM ^Ⓡ ) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM ^Ⓡ and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM ^Ⓡ , respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM ^Ⓡ kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TBFeron Plus kit was not inferior to BOVIGAM ^Ⓡ in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.