쥐의 초기 난포 발달에 관여하는 Cell Size Growth 및 CCN Family 유전자에 관한 연구

김경화,박창은,윤세진,이경아,Kim, Kyeoung-Hwa,Park, Chang-Eun,Yoon, Se-Jin,Lee, Kyung-Ah 대한생식의학회 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.3

Objectives: Previously, we sought to compile a list of genes expressed during early folliculogenesis by using cDNA microarray to investigate follicular gene expression and changes during primordialprimary follicle transition and development of secondary follicles (Yoon et al., 2005). Among those genes, a group of genes related to the cell size growth was characterized during the ovarian development in the present study. Methods: We determined ovarian expression pattern of six genes related to the cell size growth (cyr61, emp1, fhl1, socs2, wig1 and wisp1) and extended into CCN family (${\underline{c}}onnective$ tissue growth factor/${\underline{c}}ysteine$-rich 61/${\underline{n}}ephroblastoma$-overexpressed), ctgf, nov, wisp2, wisp3, including cyr61 and wisp1 genes. Expression of mRNA and protein according to the ovarian developmental stage was evaluated by in situ hybridization, and/or semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), and immunohistochemistry, respectively. Results: Among 6 genes related to the cell size growth, cyr61 and wisp1 mRNA was detected only in oocytes in the postnatal day5 mouse ovaries. cyr61 mRNA expression was limited to the nucleolus of oocytes, while wisp1 was expressed in the cytoplasm and nucleolus of oocytes, except nucleus. cyr61 mRNA expression, however, was found in granulosa cells from secondary follicles. The rest 4 genes in the cell size growth group were detected in oocytes, granulosa and theca cells. Cyr61 and Wisp1 proteins were expressed in the oocyte cytoplasm from primordial follicle stage. Especially, Cyr61 protein was detected in pre-granulosa cells, Wisp1 protein was not. By using RT-PCR, we evaluated and decided that Cyr61 protein is produced by their own mRNA in pre-granulosa cells that was not detected by in situ hybridization. cyr61 and wisp1 genes are happen to be the CCN family members. The other members of CCN family were also studied, but their expression was detected in oocytes, granulose and theca cells. Conclusions: We firstly characterized the ovarian expression of genes related to the cell size growth and CCN family according to the early folliculogenesis. Cyr61 protein expression in the pre-granulosa cells is profound in meaning. Further functional analysis for cyr61 in early folliculogenesis is under investigation.

Adenovirus에 의해서 발현된 BMP-2가 치주인대세포의 분화에 미치는 영향

김경화,박윤정,이상철,김태일,설양조,이용무,구영,한수부,정종평,류인철,Kim, Kyoung-Hwa,Park, Yoon-Jeong,Lee, Sang-Cheul,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Ku, Young,Han, Soo-Boo,Chung, Chong-Pyoung,Rhyu, In-Chul 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.2

The regeneration of lost periodontal tissue is a major goal of therapy. Periodontal ligament cell(PDL) is a specialized connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and preserve tissue homoeostasis. Bone morphogenetic proteins(BMPs) have shown much potential in the reconstruction of the periodontum by stimulate new bone and new cementum formation. Limitiations of BMP administration to periodontal lesions is high dose delivery, BMP transient biological activity, and low bioavailability of factors at the wound site. Gene delivery method can be alternative treatment strategy to deliver BMPs to periodontal tissue. The purpose of this study is to investigate efficiency of BMP-2 gene delivery with cell-based therapy using PDL cells. PDL cell were transduced with adenoviruses encoding either BMP-2 or Lac-Z gene. To evaluate osteogenic activity of expressed BMP-2 on PDL cells, we investigated secreted BMP-2, cellular activity, ALPase, produced mineralized nodules. To evaluate collagen scaffold as carrier for transduced cell delivery, we examined morphology and secreted BMP-2 of transducd PDL cells on it. BMP-2 transducd PDL cells produced higher levels of BMP-2, ALPase, mineralized nodules than non transduced cells. Cellular activity of transduced cells was showed similar activity to non transduced cells. Transduce cells attached on collagen scaffold secreted BMP-2 at 7day and was showed similar morphology to non transduced cells. These results demonstrated that transduced PDL cells produced biologically active BMP-2 and collagen scaffold could be carrier of transducd cells.

GIS사고 예방진단법의 최근 동향

김경화,Kim, Gyeong-Hwa 한국전기산업진흥회 2000 電氣産業 Vol.11 No.8

적분개념의 발달 (리만적분에서 르베그적분으로의 이행을 중심으로)

김경화,Kim, Kyung-Hwa 한국수학사학회 2008 Journal for history of mathematics Vol.21 No.3

In the 19th century Fourier and Dirichlet studied the expansion of "arbitrary" functions into the trigonometric series and this led to the development of the Riemann's definition of the integral. Riemann's integral was considered to be of the highest generality and was discussed intensively. As a result, some weak points were found but, at least at the beginning, these were not considered as the criticism of the Riemann's integral. But after Jordan introduced the theory of content and measure-theoretic approach to the concept of the integral, and after Borel developed the Jordan's theory of content to a theory of measure, Lebesgue joined these two concepts together and obtained a new theory of integral, now known as the "Lebesgue integral". 19세기에 푸리에와 디리클레가 한 개의 식으로 표현되지 않을 수도 있는 "임의의" 함수를 삼각급수로 표현하는 것과 관련하여 연속함수의 적분을 다루었던 코시의 적분보다 더 일반적인 적분의 필요성을 제기하여 리만적분론으로 이끌었다. 한동안 리만적분이 가장 일반적인 적분으로 간주되었고, 이 적분론이 집중적으로 다루어진 결과 리만적분의 약점들이 보였으나, 적어도 초기에는 이것들이 리만적분에 대한 비판으로 보이지 않았다. 그러나 죠르단이 1892년에 용량개념을 소개하며 리만적분론을 측도론적 배경에서 다루었고, 이로부터 몇 년 후에 보렐이 죠르단의 용량론을 측도론으로 발전시킨 후에 르베그가 이 둘의 이론을 합쳐서 지금 "르베그적분"으로 알고 있는 적분의 새 개념을 얻게 되었다.

서보 설비를 위한 순차적 파라미터 자동 튜닝 알고리즘을 사용한 영구자석 동기전동기의 비선형 속도 제어

김경화,Kim, Kyeong-Hwa 한국조명전기설비학회 2005 조명·전기설비학회논문지 Vol.19 No.2

서보 설비를 위한 순차적 파라미터 자동 튜닝 알고리즘을 갖는 영구자석 동기전동기(Permanent Magnet Synchronous Motor: PMSM)의 비선형 속도 제어 기법이 제시된다. 비선형 제어 방식은 시스템 파라미터와 부하상태가 정확하게 일치하지 않는 경우 만족스러운 성능을 줄 수 일다. 최근에 향상된 속도 제어 성능을 위해 적응형 기법, 슬라이딩 모드 제어, 및 관측기에 기반 한 기법들이 제안되었다. 하지만, 이 방법들은 관성의 큰 변화, 속도의 빠른 과도 특성 및 샘플링 주기 증가와 같은 특정한 조건에서 더 이상 만족스러운 성능을 제공하지 않는다. 일반적으로, 속도 동특성에 영향을 주는 주요 파라미터를 동시에 추정하기는 쉽지 않다. 이를 해결하기 위해 설치 및 기동시 전동기 주요 파라미터를 시간 구간 별로 나누어 자동으로 추정하는 순차적 파라미터 튜닝 알고리즘이 제안된다. 제안된 방식이 DSP TMS320C31을 이용하여 구현되고 유용성이 시뮬레이션과 실험을 통해 입증된다. A nonlinear speed control of a PMSM using a sequential parameter auto-tuning algorithm for servo equipments is presented. The nonlinear control scheme gives an undesirable output performance under the mismatch of the system parameters and load conditions. Recently, to improve the performance, an adaptive linearization scheme, a sliding mode control and an observer-based technique have been reported. Although a good performance can be obtained, the performance is not satisfactory any more under specific conditions such as a large inertia variation, a fast speed transient or an increased sampling time. The simultaneous estimation of principal parameters giving a direct influence on speed dynamics is generally not simple. To overcome this problem, a a sequential parameter auto-tuning algorithm at start-up is proposed, where dominant parameters are estimated in a prescribed regular sequence based on the method that one parameter is estimated during each interval. The proposed scheme is implemented on a PMSM using DSP TMS320C31 and the effectiveness is verified through simulations and experiments.

사회복지현장실습, 진정한 실습으로 거듭나야 한다

김경화,Kim, Gyeong-Hwa 한국사회복지사협회 2005 Social workers Vol.11 No.-

$LaGaO_3$:$Eu^{3+}$형광체의 합성 및 발광 특성

김경화,최윤영,손기선,김창해,박희동,최세영,Kim, Kyoung Hwa,Choi, Yoon Young,Sohn, Kee Sun,Kim, Chang Hae,Park, Hee Dong,Choe, Se Young 대한화학회 2000 대한화학회지 Vol.44 No.5

최근 정보 표시 소자로 전계 방출 표시 소자(FED),PDP.LCD 등이 주목받고 있다. 음극선 발광 형광체는 FED뿐만 아니라 형광 표시판(VFD) 등이 중요한 핵심 소자이다. 따라서 본 연구에서는 FED에 응용 가능한 새로운 모체 탐색을 시도하였고 $LaGaO_3$모체에 $Eu^{3+}$를 첨가한 적색 형광체를 합성하여 광 특성을 분석하였다. $Eu^{3+}$의 농도에 따른 발광 스펙트럼, 여기 스펙트럼과 잔광 시간 곡선을 통해 $LaGaO_3$: $Eu^{3+}$의PL거동을 규명하였다. 잘 알려진 $Eu^{3+}$ 캐스캐이딩(cascading)과 다중 음향 양자(multiphonon emisson)에 의한 cross-relaxation은 $LaGaO_3$: $Eu^{3+}$ 형광체에서도 확인되었다. 또한, Inokutti-Hirayama식으로 부터 결정된 $Eu^{3+}$ 사이의 다중극자 상호 작용(multipolar interaction)유형은 이중 극자 상호 작용(dipole-dipole interaction)으로 밝혀졌다. 본 연구를 통해 $^5D_0$ 전이의 직접 소광 기구(direct quenching mechanism)를 새롭게 제안하였다. 소광 유형은 농도에 의존하며, 0.2몰 이하에서는 확산 율속 단계에 의한 소광 현상이 우세하다가 0.3몰 이상 부터는 $^5D_0$에서 전하 이동 띠(Charge Transfer Band,CTB)로 전이되는 직접 소광 유형이 지배적이다. 음극선 거동은 800V의 가속 전압하에서 여기시켜 측정하였고, PL거동과 같이 0.125몰일때 가장 큰 615nm의 발광 휘도를 나타내었다. FED has deserved an intensive attentioD as a new flat panel display. The present investigationaims at undemtanding the photoluminescence and cathodoluminescent properties of hGaO$_3$: $Eu^{3+}$ phosphor bytaking into account the possibility that this phosphor could be applied for FED. In onler to investigate on.sucha detailed behavior; 8everM experimental skil18 Je conducted to the LaGaO$_3$:$Eu^{3+}$ phosphoL The excimtion srectrum artd emission spectmn were rnezsured in the UV range and then decay curve of $^5D_0$+$^7F_j$transitions\vas examined. The decay behavior of $^5D_0$ emission was anMyzed by a newly Iuoposed cross-relaxation mech-ani8In in asswiation with inteFwnter di1ffision (or migration). The cross-mlaxation from $^5D_0$ to CTB (Cha'geTransfer Band) wuld be a quite retsonable by considering the excitation spectrum. It could be also found thatthe quenching type was changed from ditfrsion controlled process to the direct quenching process -s inJeasing $Eu^{3+}$ oncntration.

YOBr:Eu3+ 형광체의 발광특성과 임계거리 연구

김경화,박종규,박희동,한정화,Kim, Gyeong Hwa,Park, Jong Gyu,Park, Hui Dong,Han, Jeong Hwa 대한화학회 2001 대한화학회지 Vol.45 No.6

YOB에 $Eu^{3+}$를 첨가하여 고상 반응으로 합성하였다. YOBr: $Eu^{3+}$ 형광체는 621nm에서 강한 적색 발광을 보이며, $Eu^{3+}$이온 농도가 0.05mol일 때 외대 발광 휘도를 나타냈다. 이때, 적색 발광을 유발하는 전이는 $^5D_0{\to} ^7F_2$ 이며, $Eu^{3+}$이온의 농도에 따른 발광 스펙트럼과 잔광시간 곡선을 통해 YOBr: $Eu^{3+}$형광체의 PL거동을 규명하였다. Inokuti-Hirayama식으로 잔광시간 곡선을 fitting 한 결과, YOBr내의 $Eu^{3+}$간 다중 극자 상호작용 유형(multipolar interaction)은 쌍극자-쌍극자 상효작용(dipole-dipole interaction)으로 밝혀졌다. 또한 본 연구에서는 임계거리를 구하기 위해 임계농도에 의한 계산 외에도 Inokuti-Hirayama식으로부터 fitting한 C/$C_0$값으로 구하는 방법과 $Eu^{3+}$의 여기 및 발광 스펙트럼으로 spectral overlap하여 임계거리를 구하고자 시도 하였다. 각각의 방법으로 구한 임계거리는 17.30, 10.51 및 7.18$\AA$으로 약간의 편차를 보이지만 대략적인 크기를 갖고 있어, 이는 계산이나 측정상의 오차를 감안하면 모두 임계거리를 구하는 유용한 방법으로 판단된다. The europium doped yttrium oxybromide phosphors were synthesized by solid-state reaction method. The YOBr: $Eu^{3+}$ phosphor showed a strong and narrow red emission band at 621 nm and maximum emission intensity obtained when 0.05 mol Eu ions were doped. The red emission of $Eu^{3+}$ originated from $^5D_0$ ${\rightarrow}$ $^7F_2$electric dipole transition. In order to investigate on photoluminescence behavior, several experimental skills and numerical fittings are conducted to the YOBr: $Eu^{3+}$ phosphor. The emission spectrum was measured in the UV range and then decay curve of $^5D_0$ ${\rightarrow}$ $^7F_j$transitions was examined. The energy interaction type of YOBr: $Eu^{3+}$ phosphor was dipole-dipole interaction. In addition to the calculating by critical concentration, the critical distance ($R_0$) was calculated by decay curve fitting parameter from Inokuti-Hirayamas equation, and spectral overlap method. The critical distance was 17.03, 10.51 and 7.18$\AA$ for those methods, respectively. As considering systematic error of measurements, these values are within the same order, so that the above fitting methods are plausible and recommendable.

3상 가변속 구동장치 구성의 대안

김경화,Kim, Gyeong-Hwa 전력전자학회 2007 전력전자학회지 Vol.12 No.2

자율실습에서의 상호동료 교수법 적용경험에 대한 융합적 연구: 핵심기본간호술 투약간호를 중심으로

김경화,임종미,장양민,Kim, Kyung Hwa,Lim, Jong Mi,Jang, Yang Min 한국융합학회 2021 한국융합학회논문지 Vol.12 No.9

본 연구의 목적은 상호동료 교수법을 적용한 핵심기본간호술 자율실습(투약단원을 중심으로)이 간호대학생의 학습에 미치는 영향을 심층적으로 이해하기 위함이다. 연구참여자는 간호학과 4학년 학생 15명이었으며, 심층면담을 통해 자료를 수집하였다. 수집된 자료를 분석한 결과, '학습동기 유발', '자기주도 학습', '성취도 향상', '학습요구 불충족'의 4개의 주제가 도출되었다. 상호동료 교수법을 적용한 핵심기본간호술 자율실습은 참여자들에게 편안한 환경과 자신감 향상으로 학습동기를 유발하고, 주도적인 사전학습 및 학습자의 언어로 학습할 기회를 얻는 긍정적인 경험이 있었다. 하지만, 추가적인 지식확장이 어렵고 충분하지 않은 학습요구로 불안감을 느끼는 경험도 있었다. 이에 상호동료 교수법을 자율실습에 적용할 때, 교수자는 학습자의 학습요구를 충분히 만족시키는 방안을 적용하면 효과적일 것으로 생각된다. The purpose of this study is to understand in-depth the effect of self-directed practice in for core fundamental nursing skills applying reciprocal peer tutoring on the learning of nursing college students. The study participants were 15 students in the 4th grade of the department of nursing, and data were collected through in-depth interviews. The results of analysis resulted in four themes: 'motivation of learning', 'self-directed learning', 'improving achievement', and 'insufficient learning requirements'. The core fundamental nursing skills applying reciprocal peer tutoring has a positive experience of inducing motivation of learning for participants through a comfortable environment and improving self-confidence, and gaining opportunities to lead prior learning and learn in the learner's language. However, there have been experiences where additional knowledge expansion is difficult and feelings of insecurity is felt due to insufficient learning requirements. Therefore, when applying the reciprocal peer tutoring method to self-directed practice, it is considered to be effective if the professor applies a method that can sufficiently satisfy the learner's learning needs.