Expression of the Protective Antigen for PEDV in Transgenic Duckweed, Lemna minor

고석민,선현진,오명진,송인자,김민재,신현숙,고창효,김용우,임평옥,이효연,김석원 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.5

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

내측연수경색으로 인한 반대측 중추성 안면마비

고석민 대한뇌졸중학회 2006 Journal of stroke Vol.8 No.1

The 'classic' triad of medial medullary infarction (MMI) consists of contralateral hemiplegia, loss of posterior column sensation on the contralateral side and involvement of the tongue on the ipsilateral side. Facial muscle is usually spared in MMI. A 51-year-old patient with MMI came to our department. His major clinical manifestations were contralateral hemiparesis, and contralateral central type facial palsy. He had neither sensory change nor tongue deviation. This report support the hypothesis that part of the corticobulbar fibers supplying the lower facial muscles descend ipsilaterally in the ventromedial part of the medulla and then, after decussation, ascend rostrally to the facial nucleus in the dorsolateral part of the medulla.

Implant 보철물 access hole의 깊이에 관한 연구

고석민,변태희,이재봉,Ko Sok-Min,Byun Tae-Hee,Lee Jai-Bong 대한치과보철학회 2002 대한치과보철학회지 Vol.40 No.4

A total of 605 implant fixture prosthesis delivered by 3 clinics and 2 laboratories were examined in this study, The object of this study was to determine the proper length of screw head. The depth of access hole were measured and compared to the type of fixture, abutment, gold screw and prosthesis. The results were as follows : 1 The average number of fixtures per patient were 2.97. 2. The number of fixture installed in the upper posterior area are 327(55.56 %), the upper posterior area 171 (28.25%). 3. The depth of access hole is 4.23 mm in shallow area, and 5.46 mm in deep area and the differences were 1.23 mm. 4. The average depth of the aceess hole of the UCLA abutment were 5.02 mm. 5. The number of 4-5 mm access hole depth were 60(22.39%) in abutment screw level and the number of 4-5 mm depth in fixture level were 101 (29.19%). 6. In the shape of screw head, hexed type were 576(95.21%), slotted type were 29(4.79%).

일일초에서의 기능획득 돌연변이 모상근의 대량생산

고석민,정화지,이호연 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.5

This study describes conditions for the mass production of mutant hairy root lines by co-cultivation with A. rhizogenes harboring the activation tagging vector pHC7. Various sources of explants were subjected to genetic transformation with A. rhizogenes to determine optimum conditions and cultivar for the highest frequency of hairy root formation on explants. Hairy root formation also were investigated in transformed hairy roots grown in various culture media. Finally, a total of approximately 2,500 lines of hairy root mutants were produced in this study. A managing system for metabolomics in hairy root lines also were established. These hairy root lines will be useful to determine functions of genes relating biosynthesis pathway of secondary metabolites. 본 연구는 아그로박테리움을 이용한 기능획득 일일초 모상근의 대량생산을 위한 조건 확립에 대한 것이다. 본 연구에서는, 효율적인 형질전환 일일초 모상근 생산에 있어서의 최적의 일일초 품종의 선발과 최적의 일일초 조직을 결정하였으며, 또한 다양한 배지에 있어서의 모상근 유도를 조사하였다. 최종적으로 약 2,500개의 독립적인 형질전환 일일초 모상근 line을 생산하였으며, 또한 이들을 이용하여, 대사체 연구를 위한 효율적 관리 시스템을 구축하였다. 이들 모상근 line은 일일초 인돌알칼로이드 생합성 관련 유전자의 발굴 및 기능해석에 유용하게 쓰일 것이다.

골밀도 평가방법과 임플란트 안정성 변화의 관찰

고석민,박성재,김인수,송승일,이인경,Ko, Sok-Min,Park, Sung-Jae,Kim, In-Soo,Song, Seung-Il,Lee, In-Kyung 대한치주과학회 2009 Journal of Periodontal & Implant Science Vol.39 No.2

Purpose: The aim of this study was to examine the correlation of the subjective and the objective evaluation of edentulous ridge bone quality, and to evaluate the change of the dental implant stability in each bone density group for early healing period after implant installation. Methods: Sixty-seven implants(Osstem implant$^{(R)}$, Seoul, Korea) were included in this study. We evaluated the bone density by 2 methods. The one was the subjective method which was determined by practitioner s tactile sense, the other was the objective bone type was based on Hounsfield units. The implant stability in each bone type group was assessed by resonance frequency analyzer(Osstell mentor$^{(R)}$). Data were analyzed for the change of the implant stability, and they were compared to verify the difference of groups at the time of installation, 2, 6, 10, 14 weeks postoperatively. Spearman's correlation was used to demonstrate the correlation between the subjective and the objective evaluation of the bone density, and analysis of variance(ANOVA) was used to analyze the differences of implant stability at each time point. Results: There was no close relation between the subjective and the objective evaluation of the bone density(r=0.57). In the subjective groups, there was statistically significant difference between the type 1 and 3 at 10 weeks and between the type 2 and 3 at 14 weeks. In the objective groups, there was no statistically significant difference between the D 1, 2, 3, 4, and 5 group with regard to RFA from baseline to 14 weeks(P>0.1). Conclusions: The implant stability increased over time during the study, and it was improved with bone density proportionally after 2weeks postoperatively. It is recommended that the decision of bone density is base on Hounsfield unit for implant loading time.

고령자 삼킴기능저하의 평가와 중재

고석민,이지나 대한노년치의학회 2022 대한노년치의학회지 Vol.18 No.2

Korea is on the verge of becoming a super-aged society, and the structure of the disease is changing accordingly. One of the measures to lower the medical and social burden for the elderly is early detection and prevention of elderly frailty. Oral frailty in elderly is reported to be manifested prior to whole body frailty. So, if oral frailty is diagnosed and treated early, independency in mobility and healthy aging of elderly can be effectively promoted. The Korean Academy of Geriatric Dentistry launched establishing a clinical guideline of oral frailty for Korean elderly with diagnostic criteria and treatment modality. If two out of the six criteria, chewing function, occlusal force, tongue force, salivary gland function, swallowing function, and oral hygiene, are checked as not ‘normal’ but ‘at risk’, the patient is diagnosed as having oral frailty. In this paper, evaluation methods and exercise therapies for swallowing function (out of the six criteria for oral frailty) is described in detail.

Opcode 빈도수 기반 악성코드 이미지를 활용한 CNN 기반 악성코드 탐지 기법

고석민,양재혁,최원준,김태근 한국정보보호학회 2022 정보보호학회논문지 Vol.32 No.5

As the Internet develops and the utilization rate of computers increases, the threats posed by malware keep increasing. This leads to the demand for a system to automatically analyzes a large amount of malware. In this paper, an automatic malware analysis technique using a deep learning algorithm is introduced. Our proposed method uses CNN (Convolutional Neural Network) to analyze the malicious features represented as images. To reflect semantic information of malware for detection, our method uses the opcode frequency data of binary for image generation, rather than using bytes of binary. As a result of the experiments using the datasets consisting of 20,000 samples, it was found that the proposed method can detect malicious codes with 91% accuracy. 인터넷이 발달하고 컴퓨터 이용률이 높아짐에 따라 악성코드로 인한 위협 또한 함께 증가하고 있다. 매년 발견되는 악성코드의 수는 급격히 증가하여 자동으로 대량의 악성코드를 분석하기 위한 시스템이 필요한 상황이다. 본 논문에서는 딥러닝 알고리즘을 활용한 악성코드 자동 분석 기법을 소개한다. CNN(Convolutional Neural Network)라는 이미지 분류에 활용도가 높은 알고리즘을 이용하여 악성코드의 특징을 이미지화한 데이터를 분석한다. 제안하는 방법은 악성코드의 Semantic한 정보를 탐지에 활용하기 위하여 단순 바이너리 바이트를 기반으로 생성한 이미지가 아닌, 바이너리의 명령어 빈도수를 기반으로 생성한 이미지를 CNN으로 분석한다. 악성코드 10,000개 정상코드 10,000개로 구성된 대량의 데이터 셋을 활용하여 탐지 성능을 확인한 결과, 제안하는 방법은 91%의 정확도로 악성코드를 탐지할 수 있음이 확인되었다.

Cellular Mesoblastic Nephroma with Liver Metastasis in a Neonate: Prenatal and Postnatal Diffusion-Weighted MR Imaging

고석민,김명준,임영재,박국인,이미정 대한영상의학회 2013 Korean Journal of Radiology Vol.14 No.2

Congenital mesoblastic nephroma (CMN) is the most common renal tumor in the first year of life. Here, we present unique findings of cellular variant CMN seen on prenatal and postnatal MRI with diffusion-weighted imaging (DWI).The mass was well-visualized on prenatal MR DWI with diffusion restriction in the solid portions. After excision of the mass, follow-up whole body MRI with DWI helped identify local tumor recurrence with suspicious liver metastasis. This hepatic lesion also showed diffusion restriction.

상악 총의치 장착 환자 언어의 음향학적 특성 연구

고석민,황병남 한국음성과학회 2001 음성과학 Vol.8 No.4

Speech intelligibility in patients with complete dentures is an important clinical problem depending on the material used. The objective of this study was to investigate the speech of two edentulous subjects fitted with a complete maxillary prosthesis made of two different palatal materials: chrome-cobalt alloy and acrylic resin. Three patients with complete dentures in the experiment group and ten people in the controls groups participated in the experiment. CSL, Visi-Pitch were used to measure speech characteristics. The test words consisted of a simple vowel /e/, meaningless three syllabic words containing fricative, affricated and stops sounds, and sustained fricative sounds /s/ and /??/. The analysis speech parameters were vowel and lateral formants, VOT, sound durations, sound pressure level and fricative frequency. Data analysis was conducted by a series of paired T-test. The findings like the following: (1)Vowel formant one of patients with complete denture is higher than that of the control group (p<0.05), while lateral formant three of patients with complete denture is lower than that of the control group (p<0.01). (2) Patients with complete denture produced lower speech intelligibility with low fricative frequency (/??/) than control group (p<0.0). The speech intelligibility of patients with metal prosthesis was higher than that of those with resin prosthesis (p<0.05). (3) Fricative, lateral and stop sound durations of patients with complete denture were longer than those of the control group (p<0.01 and p<0.05), respectively. Total sound durations of patients with metal prosthesis were similar to that of the control group (p<0.05), while those with resin prosthesis had a shorter duration (p<0.01). This implied that those with metal prosthesis had higher speech intelligibility than those with resin prosthesis. (4) Patients with complete denture had higher sound pressure levels /t/ and /c/ than the control group (p<0.01). However, sound pressure levels for /c/ of patients with metal prosthesis or resin prosthesis was similar to the control group (p<0.05). (5) Patients with complete denture had higher fundamental frequency than the control group (p<0.01).

Photonic diode behavior in energy-graded GaN/InGaN core-shell quantum well rod grown by metal-organic chemical vapor deposition

고석민,공수현,조용훈 한국진공학회 2014 한국진공학회 학술발표회초록집 Vol.2014 No.8